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51.
Gesunde Pflanzen - Alternate wetting and drying irrigation (AWDI) is a water-conserving strategy in rice fields. An experiment, conducted as a split-split plot design and based on...  相似文献   
52.
Gesunde Pflanzen - Pistachio (Pistacia vera L.) is an economically important nut crop that is prone to drought stress since it is mainly produced in arid and semi-arid regions. Considering...  相似文献   
53.
Gesunde Pflanzen - Humic acid comprises the major part of humic substances and the most important component of soil organic matter (humus) that increases crop yield and quality. This research aimed...  相似文献   
54.
The primary goal of this project was to establish a protocol to freeze the sperm of the Caspian miniature horse in an attempt to start an intensive artificial insemination program to effectively increase the population of this breed, which has been listed as “Critical Rare Breed” by the American Livestock Breed Conservancy and is in danger of extinction. Commercially available equine freezing medium (EquiPRO CyoGuard Complete egg-yolk extender) was used for the initial setup of two different freeze protocols: slow and fast. The fast-freeze protocol had slightly better postthaw results and was used for a fertility demonstration. Five mares of proven fertility, aged 3 to 12 years, were used in the fertility trials, two of which resulted in pregnancy. This is the first report of pregnancy in the Caspian miniature horse using frozen semen, and the results seem to be a promising start to an extensive program to help this endangered breed, although further research on freezing protocols and conditions for this process are necessary to further improve the survival of semen and pregnancy rate.  相似文献   
55.
Persian sturgeon eggs were fertilized with different levels of salinities (0.5, 2, 4, 6, 8, 10, 12?ppt), and then each group was incubated in the same salinity until hatch. The fertility (%), hatching rate as well as larvae cumulative mortality rate and abnormality (%) were measured. Our Results revealed that Persian surgeon eggs could be fertilized in the different salinity concentrations but not more than 4?ppt. Moreover, hatching rate decreased with increase in salinities more than 2 and 4?ppt, respectively, and no larvae hatched in 6?ppt salinity. According to these results, the salinity tolerance threshold for Persian sturgeon larvae hatching in brackish water is less than 4?ppt.  相似文献   
56.
Selenium (Se) is an essential element for human and livestock with antioxidant and anticancer characteristics. Although Se is not an essential element for plants, it has been reported that it can improve plant growth. This experiment was conducted at the Isfahan University of Technology in winter 2010. The experiment was factorial based on a completely randomized design (CRD) with four replications. Se was added to nutrient solution in four concentrations 2, 4, and 6 mg/L sodium selenite (Na2SeO3). Root volume, fresh and dry weights of shoots and roots, number and weight of fruits, chlorophyll content, and photosynthesis traits [photosynthesis rate, stomata internal carbon dioxide (CO2) concentration, stomata conductance] were measured. Results showed that Se increased root dry weight. Fresh and dry weights of shoot increased in the 2 mg/L Se treatment and decreased at the higher level of Se. Chlorophyll content and photosynthesis rate were not affected by Se. Stomata internal CO2 concentration and stomata conductance decreased by Se addition. Overall, Se at 2 mg/L application rate was effective in some physiological characteristics of cucumber.  相似文献   
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58.
The present research aimed at evaluating the effects of sodium selenite and selenium nanoparticles (Se NPs) on iron homeostasis and the expression of transferrin and its receptor-binding protein genes. Twenty one Lori-Bakhtiary sheep were randomly allocated into 3 groups. Groups 1 and 2 orally received Se NPs and sodium selenite (1 mg kg(-1)) for 10 consecutive days, respectively. Group 3 served as the control. Blood and sternal bone marrow samples were collected at different supplementation intervals. Various factors such as serum iron concentration, total iron binding capacity (TIBC), and transferrin saturation percent were determined. The expression of transferrin and transferrin binding receptor genes was also studied. Results showed a decreasing trend in serum iron concentration particularly during the early and middle stages of supplementation (0-20 days) with Se NPs or selenium ions. Conversely, the TIBC level increased in sera especially during these periods (0-20 days) in animals that received selenium NPs or selenium ions. Our results also showed that expression of transferrin and its receptor genes was considerably increased during supplementation of the animals by both selenium compounds for 10 or 20 days. After this period, the expression of the mentioned genes significantly decreased, especially in animals that received selenium ions.  相似文献   
59.
In the current study we aimed to use PCR to investigate the presence of fetal DNA in the bovine (Bos taurus) cervical secretions and maternal serum, and to assess the effectiveness of this method in fetal gender determination. Pregnant uteri and pre-slaughter maternal blood samples were collected from 21 Holstein Frisian cows in a local abattoir. Overall, 13 male and 8 female fetuses were included in the study. Cervical mucus was sampled at the laboratory. After DNA extraction, the PCR amplified a 280?bp fragment from the X-chromosome and a 217?bp fragment from the Y-chromosome based on a sex-related polymorphism in the amelogenin locus. The presence of fetal Y-chromosome was confirmed in seven out of 13 cervical mucus samples collected from cows with male fetuses. Overall test sensitivity for correct sex determination based on PCR assay on cervical samples was equal to 71.4?±?2?%. In contrast, no fetal Y-chromosome DNA was detected in maternal serum samples from cows with male fetuses. This is the first report on validating the presence of fetal DNA material in the bovine cervical mucus and its potential usefulness for fetal sexing. Further investigations are needed to maximize the accuracy and evaluate the practicality of this approach.  相似文献   
60.
Two diploid sugar beet genotypes of agronomical importance were transformed using Agrobactrium tumefaciens harboring pBI35Scry containing a synthetic cry1Ab gene. Leaf blade with attached shoot bases, a highly regenerative tissue, were used as explant substratum for transformation. PCR screening with cry1Ab-specific primers showed the presence of transgene in more than 50% of the regenerated kanamycin-resistant plants after treatment with the antibiotic. A transformation rate of 8.8–12.2% (depending on genotype) was achieved as revealed by genomic DNA dot blotting. The intact integration of transgene cassette into the genome was furthermore confirmed by Southern blot analysis. The expression of the cry1Ab gene encoding a truncated endotoxin (67 kDa) at about 0.1% of total soluble protein was achieved in the leaves of transgenic plants as shown by Western blot analysis. Bioassays under in vitro conditions with Spodoptera littoralis, one of the most important pests in sugar beet fields, demonstrated enhanced resistance against this pest. The inheritance of the inserted transgene was confirmed in F1 plants obtained through crossing of T0 plants with a cytoplasmic male sterile line. Transgenic plants are currently grown in a greenhouse and will be subjected to further bioassay analyses against other lepidopteran pests of sugar beet.  相似文献   
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