Detection of <Emphasis Type="Italic">Trypanosoma vivax</Emphasis> DNA in semen from experimentally infected goats     

Nicholas Morais Bezerra  Gabriela Hémylin Ferreira Moura  Hélio Noberto de AraújoJr.  Francisco Silvestre Brilhante Bezerra  Kaliane Alessandra Rodrigues de Paiva  Kizzy Millenn de Freitas Mendonça Costa  Wirton Peixoto Costa  Dayse Ariane Soares Medeiros  Jael Soares Batista 《Veterinary research communications》2018,42(2):131-135

The present work aimed to investigate the presence of T. vivax DNA in the semen of experimentally infected goats. Twelve male goats native to the Brazilian Northeast, adults, were randomly assigned to two experimental groups: the infected group consisting of six goats infected intravenously with 0.5 mL of blood containing approximately 1.25?×?10⁵ trypomastigotes of T. vivax, and a control group composed of six uninfected goats. After the infection, clinical examinations aiming to evaluate rectal temperature, parasitemia and hematocrit were performed. Semen samples were collected from goats by electroejaculation on the 7th, 14th and 21st days post-infection (dpi). The recombinant DNA-encoding gene encoding the L-like-specific gene for T. vivax. The infection was characterized by increased rectal temperature, high parasitemia and significant reduction of hematocrit values. Results for T. vivax DNA detection using TviCatL-PCR were positive in all semen samples from the infected group collected on 7th, 14th and 21st dpi. The presence of T. vivax DNA in 7th dpi suggests the early invasion of the parasite in the reproductive organs. Also, the finding of T. vivax DNA in all periods analyzed may suggest the continued elimination of the parasite in the semen, which may increase the chances of sexual transmission. Thus, T. vivax DNA is recorded for the first time in the semen of infected goats. Thus, these data are of great importance, since the detection of the T. vivax genetic material in the semen may point to the possibility that the parasite may be transmitted through the sexual pathway.  相似文献   

Does the level of forage neutral detergent fiber affect the ruminal fermentation,digestibility and feeding behavior of goats fed cactus pear?          下载免费PDF全文

Ricardo Martins Araujo Pinho  Edson Mauro Santos  Juliana Silva de Oliveira  Gleidson Giordano Pinto de Carvalho  Thiago Carvalho da Silva  Alberto Jefferson da Silva Macêdo  Yohana Rosaly Corrêa  Anderson de Moura Zanine 《Animal Science Journal》2018,89(10):1424-1431

This study aimed to evaluate the effects of forage neutral detergent fiber (fNDF) levels on the voluntary feed intake, digestibility, ruminal fermentation and feeding behavior of goats fed diets with cactus pear. Five non‐lactating ruminally cannulated goats fed ad libitum were randomly assigned to a 5 × 5 Latin square design. Treatments consisted of levels of fNDF at 0, 109, 222, 339 and 463 g/kg of dry matter (DM) in cactus pear‐based diets. The intakes of DM and NDF were quadratically affected (p ≦ .045) by fNDF levels. Voluntary water intake (VWI) increased linearly as the fNDF levels increased in the diet. The digestibility coefficients of organic matter, NDF and ether extract and total digestible nutrients concentration were quadratically affected (p ≦ .048) by fNDF levels. The ruminal pH linearly increased (p = .001) with fNDF levels, ranging from 5.44 to 5.81 for diets containing 0 and 463 g fNDF/kg DM, respectively. The fNDF levels promoted a linear increase (p = .006) in chewing time, linearly decreased (p = .007) resting time and quadratically affected (p = .033) rumination time. The inclusion of fNDF in the diets provided favorable conditions for ruminal function, digestibility and feeding behavior in goats fed diets containing cactus pear.  相似文献   

Virulence factors in Escherichia coli strains isolated from urinary tract infection and pyometra cases and from feces of healthy dogs     

Siqueira AK  Ribeiro MG  Leite Dda S  Tiba MR  Moura Cd  Lopes MD  Prestes NC  Salerno T  Silva AV 《Research in veterinary science》2009,86(2):206-210

The aim of this study was to compare the prevalence of virulence genes in 158 Escherichia coli strains isolated from 51 clinical cases of UTIs, 52 of pyometra and from 55 fecal samples from healthy dogs by PCR. papC was found in 12 (23.5%) strains isolated from UTIs, 19 (36.5%) from pyometra and 10 (18.2%) from feces. papGII was observed in 3 (5.8%) strains from pyometra, and papGIII in 10 (19.6%) from UTIs, 15 (28.8%) from pyometra and 9 (16.4%) from feces. sfaS was detected in 22 (43.1%) strains from UTIs, 24 (46.1%) from pyometra and 19 (34.5%) from feces. hlyA was observed in 17 (33.3%) strains from UTIs, 18 (34.6%) from pyometra and 7 (12.7%) from feces, while cnf-1 was detected in 11 (21.6%) from UTIs, 21 (40.4%) from pyometra and 9 (16.4%) from feces. iucD was observed in 12 (23.5%) strains from UTIs, 9 (17.3%) from pyometra and 1 (1.8%) from feces. usp was found 17 (33.3%) isolates from UTIs and 36 (69.9%) from pyometra.  相似文献   

Parasitic encephalitis caused by Stephanurus dentatus in a pig in Brazil     

Robert G. S. Prado  Chris H. Gardiner  Mrcio A. O. Moura  Gerson B. E. Gonzalez  Marcos D. Duarte  Tiago F. S. Santos  Pedro S. Bezerra  Valíria D. Cerqueira  Alessandra S. Amaral  Gabriela Riet-Correa 《Journal of veterinary diagnostic investigation》2021,33(5):949

A pig was in left lateral recumbency with limb spasticity, accentuated prostration, and strabismus, and was euthanized. During autopsy, yellowing of the leptomeninges at the ventral pons to medulla oblongata was noted. In the cerebellar peduncles, there was a focally extensive black-to-yellow area at the level of the vestibular nuclei. Histologic examination revealed a cross-section of a nematode larva, consistent with Stephanurus dentatus, bordered by edema and marked infiltration of mononuclear cells, plasma cells, and a few eosinophils. Vacuolation of the neuropil, with rare gitter cells and axonal spheroids, was also observed. We diagnosed parasitic encephalitis caused by S. dentatus migration based on the pathology findings and characterization of the parasite.  相似文献   

Long Chain Fatty Acids of Diet as Factors Influencing Reproduction in Cattle   总被引：1，自引：0，他引：1  

JEP Santos  TR Bilby  WW Thatcher  CR Staples  FT Silvestre 《Reproduction in domestic animals》2008,43(S2):23-30

Cattle are fed moderate amounts of long chain fatty acids (FA) with the objective to enhance lactation and growth; however, recent interest on lipid feeding to cows has focused on reproduction, immunity and health. Increasing the caloric density of the ration by fat feeding has generally improved measures of cow reproduction, but when milk yield and body weight losses were increased by fat supplementation, positive effects on reproduction were not always observed. Feeding fat has influenced reproduction by altering the size of the dominant follicle, hastening the interval to first postpartum ovulation in beef cows, increasing progesterone concentrations during the luteal phase of the oestrous cycle, modulating uterine prostaglandin (PG) synthesis, and improving oocyte and embryo quality and developmental competence. Some of these effects were altered by the type of FA fed. The polyunsaturated FA of the n-6 and n-3 families seem to have the most remarkable effects on reproductive responses of cattle, but it is not completely clear whether these effects are mediated only by them or by other potential intermediates produced during rumen biohydrogenation. Generally, feeding fat sources rich in n-6 FA during late gestation and early lactation enhanced follicle growth, uterine PG secretion, embryo quality and pregnancy in cows. Similarly, feeding n-3 FA during lactation suppressed uterine PG release, and improved embryo quality and maintenance of pregnancy. Future research ought to focus on methods to improve the delivery of specific FA and adequately powered studies should be designed to critically evaluate their effects on establishment and maintenance of pregnancy in cattle.  相似文献   

Genetic variability of avian Escherichia coli strains evaluated by enterobacterial repetitive intergenic consensus and repetitive extragenic palindromic polymerase chain reaction   总被引：2，自引：0，他引：2  

Carvalho de Moura AC  Irino K  Vidotto MC 《Avian diseases》2001,45(1):173-181

In this study, we tested the capability of enterobacterial repetitive intergenic consensus (ERIC) and repetitive extragenic palindromic (REP) polymerase chain reaction (PCR) to detect genetic diversity among Escherichia coli strains isolated from chickens bearing clinical signs of colibacillosis and compared the genotypes so obtained with the O:H serotypes and virulence of those strains. The DNAs from 50 avian E. coli strains and from E. coli ATCC 25922 were used to amplify ERIC and REP sequences. DNA from avian strains produced from 8 to 17 bands by ERIC-PCR and from 6 to 20 bands by REP-PCR; E. coli ATCC produced 11 bands by both methods. ERIC and REP-PCR showed good discriminating power, and the dendograms based on the different patterns revealed extensive genetic diversity among the avian strains. Those strains were allocated into four major clonal clusters, each one with 60% of similarity by ERIC and REP-PCR, and those clusters corresponded to strains with different degrees of pathogenicity. However, 56% of the pathogenic strains (28/50) belonged to two out of three major clonal clusters, and 86% of the nonpathogenic strains tended to group in one cluster and one subgroup. The 32 serotypes detected were distributed in all clusters, and within a serogroup, different DNA fingerprints were observed; however, strains with same serotypes tended to form clusters with similarity coefficients greater than 80%. These results suggest that no specific serotype and genotype is responsible for colibacillosis and that REP and ERIC-PCR are reproducible techniques that can improve the studies needed to clarify the pathways to the pathogenesis of colibacillosis.  相似文献   

Parameters of the Reproductive Tract,Spermatogenesis, Daily Sperm Production and Major Seminal Plasma Proteins of Tropically Adapted Morada Nova Rams     

FML Sousa  CH Lobo  ESB Menezes  JPA Rego  RV Oliveira  AC Lima‐Souza  M Fioramonte  FC Gozzo  RCFF Pompeu  MJD Cândido  JT Oliveira  AA Moura 《Reproduction in domestic animals》2014,49(3):409-419

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The end of a Chilean institute     

Barbeito L  Chun J  Binder LI  Neto VM  Perry G  Scazzochio C  Violini G 《Science (New York, N.Y.)》2005,308(5723):792-793

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Proteomic profile of pre-implantational ovine embryos produced in vivo     

Deisy J. D. Sanchez  Fabio R. Vasconcelos  Antônio C. A. Teles-Filho  Arabela G. A. Viana  Aline M. A. Martins  Marcelo V. Sousa  Mariana S. Castro  Carlos A. Ricart  Wagner Fontes  Marcelo Bertolini  Ivan C. Bustamante-Filho  Arlindo A. Moura 《Reproduction in domestic animals》2021,56(4):586-603

The present study was conducted to decipher the proteome of in vivo-produced pre-implantation ovine embryos. Ten locally adapted Morana Nova ewes received hormonal treatment and were inseminated 12 hr after ovulation. Six days later, 54 embryos (morula and blastocyst developmental state) were recovered from eight ewes and pooled to obtain sufficient protein for proteomic analysis. Extracted embryo proteins were analysed by LC-MS/MS, followed by identification based on four database searches (PEAKS, Proteome Discoverer software, SearchGUI software, PepExplorer). Identified proteins were analysed for gene ontology terms, protein clusters and interactions. Genes associated with the ovine embryo proteome were screened for miRNA targets using data sets of TargetScan ( http://www.targetscan.org ) and mIRBase ( http://www.mirbase.org ) servers. There were 667 proteins identified in the ovine embryos. Biological processes of such proteins were mainly related to cellular process and regulation, and molecular functions, to binding and catalytic activity. Analysis of the embryo proteins revealed 49 enriched functional clusters, linked to energy metabolism (TCA cycle, pyruvate and glycolysis metabolism), zona pellucida (ZP), MAPK signalling pathway, tight junction, binding of sperm to ZP, translation, proteasome, cell cycle and calcium/phospholipid binding. Sixteen miRNAs were related to 25 pre-implantation ovine embryo genes, all conserved in human, bovine and ovine species. The interaction network generated by miRNet showed four key miRNAs (hsa-mir-106b-5p; hsa-mir-30-5p; hsa-mir-103a-5p and hsa-mir-106a-5p) with potential interactions with embryo-expressed genes. Functional analysis of the network indicated that miRNAs modulate genes related to cell cycle, regulation of stem cell and embryonic cell differentiation, among others. Retrieved miRNAs also modulate the expression of genes involved in cell signalling pathways, such as MAPK, Wnt, TGF-beta, p53 and Toll-like receptor. The current study describes the first major proteomic profile of 6-day-old ovine embryos produced in vivo, setting a comprehensive foundation for our understanding of embryo physiology in the ovine species.  相似文献   

Plant regeneration from protoplasts isolated from primary calli using mature embryos of two Brazilian rice cultivars     

Daniel S. Moura  Francisco J. Zapata-Arias  Akihiko Ando  Augusto Tulmann Neto 《Euphytica》1997,94(1):01-05

A plant regeneration system from rice protoplasts using calli derived from mature embryos was established for the two Brazilian modern rice cultivars IAC-201 and IAC-165. After 30 to 40 days of in vitro culture it was possible to obtain on average 6 million protoplasts per gram of callus. Microscopic selection of embryogenic calli was a key step for protoplast isolation. The production of embryogenic calli increased when L-proline and casein hydrolysate were used in the callus induction medium. The Oc or IR52 nurse cell lines were essential for protoplast division. Different regeneration media were studied and 139 plants were regenerated which set seed. Some of the regenerated plants showed morphological variation such as the presence of awns in spite of the short time of the in vitro culture. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献