Towards developing resistance to chickpea chlorotic dwarf virus through CRISPR/Cas9-mediated gene editing using multiplexed gRNAs     

Malik  Muniba Abid Munir  Haider  Muhammad Saleem  Zhai  Ying  Khan  Muhammad Azmat Ullah  Pappu  Hanu R. 《植物病害和植物保护杂志》2023,130(1):23-33

Journal of Plant Diseases and Protection - Mastreviruses are an emerging group of viruses transmitted by leafhoppers and infect both monocot and dicot plants. Chickpea chlorotic dwarf virus...  相似文献   

Hydraulic model investigation of downstream erosion of large regulators: a case study     

Muhammad F. Bari 《Irrigation and Drainage Systems》1993,7(2):131-150

Flow conditions, which could not be explained, occurred in the stilling basin and outfall channel of the Feni Regulator sited at the western end of the Feni River closure dam. This regulator controls outflows from the upstream reservoir which supplies irrigation water to Muhuri Project in Bangladesh. Analysis of flood discharge data revealed that the design discharge for the structure was not exceeded; yet abnormal scour occurred in the outfall channel and the brick block rip-rap placed thereon was damaged. A model study was conducted to understand the causes of such unusual local erosion downstream of the stilling basin and to provide answers to two main questions:

-Is potential scour serious in terms of the stability of the structure?

-What protection measures could be taken to stabilize the scour at a safe level?

Using a 1:30 scale model, the probable maximum scour was simulated, and the performance of alternative rip-rap designs including that of the existing one were examined. The results of this study supplemented by field scour data collected during subsequent flood seasons indicated that even if the flow rate through the regulator approaches the design flood discharge, the downstream scour is not likely to extend up to an elevation of ? 10.7 m, a scour level observed in the previous year at a lower discharge. It also showed that the existing rip-rap blocks were marginally undersized and consequently the rip-rap was prone to failure if flow conditions departed from uniform. A suitable method of scour protection downstream of the stilling basin at the regulator exit also evolved from the study.  相似文献   

Effects of silvopastoral areas on milk production at dual-purpose cattle farms at the semi-humid old agricultural frontier in central Nicaragua     

Wataru Yamamoto  Ioan Ap Dewi  Muhammad Ibrahim 《Agricultural Systems》2007

In extensive cattle production systems, the composition of grazing areas may significantly influence productivity. In dual-purpose cattle production systems in the lowland tropics, pasture lands with trees, so-called silvopastoral areas, are considered as being important, particularly to facilitate the management of crossbred European native cattle. The aim of the study was to quantify the effects of silvopastoral areas on production at dual-purpose cattle farms in the semi-humid lowlands of central Nicaragua. The relationships between seasonal milk production and herd data, and the proportions of land use types were examined for 74 farms by stepwise regression analysis.  相似文献   

Impacts of Institutional Reforms on Irrigated Agriculture in Pakistan     

Muhammad Latif  Muhammad Saleem Saleem Pomee 《Irrigation and Drainage Systems》2003,17(3):195-212

Poor functioning of theirrigation system in Pakistan has been asource of concern for the last few decades,during which time it has been the subject ofconsiderable external assistance andinternal policy reforms. Consequently, thegovernment of Pakistan introducedinstitutional restructuring in irrigationand drainage subsectors to impartnecessary improvements. Under thesereforms, management at secondary canallevel has recently been handed over to theFarmers Organizations (FOs) of selecteddistributaries in pilot areas. This studywas designed to evaluate a farmer-manageddistributary in southern Punjab. Fieldmeasurements revealed that hydraulicaspects of the irrigation service deliveredby the FO management has been significantlyimproved and as a result highlyproportionate and equitable waterdistribution was observed, particularly atthe tail reaches of the distributary. Dueto effective FO management farmers'interventions to increase the outletdischarge by illegal means has been almosteradicated. As a result of theseimprovements imparted by the FO in systemmanagement, the extent of irrigated area hasincreased on average by 6 to 7%, evenunder severe drought-like conditionsprevailing in the country during recentyears. Further cost recoveries haveincreased by an amount of 14% for summerand 23% for winter growing seasons,respectively, from the irrigated area of thedistributary. Thus experience from Hakra4-R distributary has shown that even underundesirable natural circumstancesIrrigation Management Transfer (IMT) wasquite effective in achieving the keytargets of the institutional reformslaunched at pilot scale in the country.  相似文献   

A study of water distribution from a branch to distributary canals: A case study of Gugera Branch,Punjab, Pakistan     

Muhammad Nawaz Bhutta  Muhammad Latif  Jacob W. Kijne 《Irrigation and Drainage Systems》1991,5(3):229-247

This study was conducted on Gugera Branch of Lower Chenab Canal, Punjab, Pakistan. Sample distributaries off taking from Gugera Branch were selected for the study. The existing conditions of water distribution among the distributaries were studied. Field data were collected during the whole of 1988. Field observations suggested that the variability at the head of distributaries is much greater than the variability in the Gugera Branch under existing operational practices. The distribution of water among the distributaries is rarely in accordance with design criteria. Some channels get priority over other channels. The annual closure period varied from 17 to 41 days for different channels. The discharge at the head of distributaries remained lower than the standard operational range for 69 to 183 days in a year. The data suggested that a regulating gate at the head of the distributary can reduce discharge variation up to 2.4 times compared with a Karrees System (wooden stop logs used for water regulation). The data indicated that the adjustments in the head gate of a distributary on daily basis can substantially improve discharge conditions at the head of distributary. Rotational schedules are not being followed as per design and need to be improved. Most of the existing head discharge relationships of discharge measuring structures are not reliable. A frequent calibration of these structures is recommended.  相似文献   

Studies on Specific Inhibitors and Substrates of Cholinesterases of Fasciola gigantica from Cattle and Buffaloes     

M. S. Durrani  Muhammad Nawaz  N. I. Chaudhary 《Zoonoses and public health》1982,29(8):636-641

  相似文献   

Evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls     

Leila Feiz  Muhammad Irshad  Rafael F Pont-Lezica  Hervé Canut  Elisabeth Jamet 《Plant methods》2006,2(1):10-13

Background  

The ultimate goal of proteomic analysis of a cell compartment should be the exhaustive identification of resident proteins; excluding proteins from other cell compartments. Reaching such a goal closely depends on the reliability of the isolation procedure for the cell compartment of interest. Plant cell walls possess specific difficulties: (i) the lack of a surrounding membrane may result in the loss of cell wall proteins (CWP) during the isolation procedure, (ii) polysaccharide networks of cellulose, hemicelluloses and pectins form potential traps for contaminants such as intracellular proteins. Several reported procedures to isolate cell walls for proteomic analyses led to the isolation of a high proportion (more than 50%) of predicted intracellular proteins. Since isolated cell walls should hold secreted proteins, one can imagine alternative procedures to prepare cell walls containing a lower proportion of contaminant proteins.  相似文献   

Foliar Application of Glycinebetaine and Salicylic Acid Improves Growth,Yield and Water Productivity of Hybrid Sunflower Planted by Different Sowing Methods     

M. Hussain  M. Farooq  K. Jabran  A. Wahid 《Journal of Agronomy and Crop Science》2010,196(2):136-145

Improved planting methods and foliar application of glycinebetaine (GB) and salicylic acid (SA) can improve the water productivity in field crops under limited water supply conditions. A 2‐year field study was conducted to evaluate the possible role of different planting methods and foliar applications of GB and SA in improving the yield, quality and water productivity of hybrid sunflower (Helianthus annuus L.). The crop was planted by flat sowing (75 cm spaced rows) and ridge sowing (75 cm spaced ridges), with GB and SA applied exogenously at 100 and 0.724 mm , respectively, at both budding and flowering stages, while control plots received distilled water. Ridge sowing, rather than flat sowing, improved the biological yield, oil yield, leaf area index (LAI), crop growth rate (CGR), plant height, water‐use efficiency and final achene yield during both the years. Foliar applications of GB and SA at both the stages improved the achene yield, although foliar application of GB at flowering was the most effective. Neither the planting methods nor the foliar application of GB and SA altered the achene oil contents during both the years. Foliar application of GB and SA increased the free proline content of the leaf and GB contents at flowering but reduced the achene protein contents, whereas planting method had no effect on these attributes across the years. Of the foliar applied chemicals, GB was more effective in improving sunflower growth and yield and water productivity than SA. To conclude, ridge sowing coupled with foliar application of GB at flowering stage could be beneficial for achieving maximum yields of hybrid sunflower under relatively water limited conditions in the field.  相似文献   

Glycinebetaine Improves Chilling Tolerance in Hybrid Maize     

M. Farooq  T. Aziz  M. Hussain  H. Rehman  K. Jabran  & M. B. Khan 《Journal of Agronomy and Crop Science》2008,194(2):152-160

Plant growth and development is hampered by various environmental stresses including chilling. We investigated the possibility of improving chilling tolerance in hybrid maize by glycinebetaine (GB) seed treatments. Maize hybrid (Hycorn 8288) seeds were soaked in 50, 100 and 150 mg l^?1 (p.p.m.) aerated solution of GB for 24 h and were dried back. Treated and untreated seeds were sown at 27 °C (optimal temperature) and at 15 °C (chilling stress) under controlled conditions. Germination and seedling growth was significantly hindered under chilling stress. Moreover, chilling stress significantly reduced the starch metabolism and relative water contents (RWC), and increased the membrane electrolyte leakage. However, activities of antioxidants (catalase, superoxide dismutase and ascorbate peroxidase) were increased under stress conditions. Seed treatments with GB improved the germination rate, root and shoot length, seedling fresh and dry weights, leaf and root scores, RWC, soluble sugars, α‐amylase activity and antioxidants significantly compared with untreated seeds under optimal and stress conditions. Induction of chilling tolerance was attributed to maintenance of high tissue water contents, reduced membrane electrolyte leakage, and higher antioxidant activities and carbohydrate metabolism. Seed treatment with 100 mg l^?1 GB was the best treatment for improving the performance of hybrid maize under normal and stress conditions compared with control and other levels used.  相似文献   

Wheat Seed Presoaking for Improved Germination     

Shahbaz Ahmad  Muhammad Anwar  Hameed Ullah 《Journal of Agronomy and Crop Science》1998,181(2):125-127

Seed presoaking improves wheat germination under marginal moisture conditions. The duration of seed presoaking was studied at The University of Arid Agriculture, Rawalpindi, using 10 wheat varieties. Seed presoaking beyond 12 h does not improve germination further, and beyond 21 h, germination rate is drastically reduced.  相似文献