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91.
Rollover protective structures (ROPS) are an effective engineering control known to prevent tractor overturn deaths, the leading cause of occupational fatalities for farmers and farm workers in the U.S. However, the use of ROPS is known to vary greatly from farm to farm. A national sample of 11,458 farm operators from the 2004 Occupational Injury Surveillance of Production Agriculture (OISPA) survey was used to assess the association between the prevalence of ROPS and ten farm operator and farm demographic variables using logistic regression. The variable were: operator's age, operator's sex, operator's education, farm sales, full- or part-time farming, acreage, type of operation, number of hired workers, number of injuries, and region. All ten variables were found to have significant associations with the prevalence of non-ROPS tractors on farms in the univariate logistic regressions. For the multivariate model, all variables except for the sex of the farm operator remained significant. Farms with less than three adult injuries, no hired workers, less than 300 acres in size, a Midwest location, and a primary farm type of tobacco, fruit and nuts, dairy, or poultry and eggs all had adjusted odds ratios of 2 or greater. Increasing the prevalence of ROPS-equipped tractors is essential for reducing the leading cause of death on farms, tractor overturns. Economic factors play a major role in the prevalence and distribution of non-ROPS tractors on farms. The identified associations can be used to effectively target areas of the U.S. for ROPS promotion activities.  相似文献   
92.
Objective-To develop in genetically engineered mice an alternative screening method for evaluation of P-glycoprotein substrate toxicosis in ivermectin-sensitive Collies. Animals-14 wild-type C57BL/6J mice (controls) and 21 genetically engineered mice in which the abcb1a and abcb1b genes were disrupted and the mutated canine ABCB1 gene was inserted. Procedures-Mice were allocated to receive 10 mg of ivermectin/kg via SC injection (n = 30) or a vehicle-only formulation of propylene glycol and glycerol formal (5). Each was observed for clinical signs of toxic effects from 0 to 7 hours following drug administration. Results-After ivermectin administration, considerable differences were observed in drug sensitivity between the 2 types of mice. The genetically engineered mice with the mutated canine ABCB1 gene had signs of severe sensitivity to ivermectin, characterized by progressive lethargy, ataxia, and tremors, whereas the wild-type control mice developed no remarkable effects related to the ivermectin. Conclusions and Clinical Relevance-The ivermectin sensitivity modeled in the transgenic mice closely resembled the lethargy, stupor, disorientation, and loss of coordination observed in ivermectin-sensitive Collies with the ABCB1-1Δ mutation. As such, the model has the potential to facilitate toxicity assessments of certain drugs for dogs that are P-glycoprotein substrates, and it may serve to reduce the use of dogs in avermectin derivative safety studies that are part of the new animal drug approval process.  相似文献   
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94.
Ammonification of soil organic N and nitrification of ammonium-N was studied in Tindall clay loam over a range of temperatures from 20–60 C. Nitrification rates at each temperature were constant throughout the 28 day incubation, whereas most of the ammonification occurred in the first 7 days. The optimum for nitrification was close to 35 C. exhibiting a sharp peak at this temperature at which the potential rate was 4.8 μg N/g day?1, compared with 0.5 μg N/g day?1 at 20°C and 0.25 μg N/g day?1 at 60°C. The optimum temperature for ammonification was approximately 50°C at which the rate was 2.8 μg N/g day?1 in the first 7 days but only 0.5 μg N/g day?1 between 14 and 28 days.The temperature responses could be described mathematically with functions of the type logoN = k × 1/T.The results are discussed in relation to daily patterns of N mineralization in the field where temperatures show diurnal fluctuation.  相似文献   
95.
For simulating hierarchically structured raster maps of landscapes that consist of multiple land cover types, we extend the concept of neutral landscape models to provide a general Markovian model. A stochastic transition matrix provides the probability rules that govern landscape fragmentation processes by assigning finer resolution land cover categories, given coarser resolution categories. This matrix can either be changed or remain the same at different resolutions. The probability rules may be defined for simulating properties of an actual landscape or they may be specified in a truly neutral manner to evaluate the effects of particular transition probability rules.For illustration, model parameters are defined heuristically to simulate properites of actual watershed-delineated landscapes in Pennsylvania. Three landscapes were chosen; one is mostly forested, one is in a transitional state between mostly forested and a mixture of agriculture, urban and suburban land, while the third is fully developed with only remnant forest patches that are small and disconnected. For each landscape type, a small sample of raster maps are simulated in a Monte Carlo fashion to illustrate how an empirical distribution of landscape measurements can be obtained.  相似文献   
96.
OBJECTIVES: To determine whether there is a relationship between species-specific mitochondrial DNA (mtDNA), especially canine and feline mtDNA, and detectable amounts of pentobarbital in previously analyzed dog food samples. SAMPLE POPULATION: 31 dog food samples previously analyzed for pentobarbital (limit of detection, 1 microg/kg). PROCEDURE: Polymerase chain reaction (PCR) analysis was performed on dog food samples by use of PCR primers specific for either canine, feline, equine, bovine, porcine, ovine, or poultry mtDNA. RESULTS: PCR amplicons specific for feline or canine mtDNA at a 0.007% (70 microg/g [wt/wt basis]) or 0.0007% (7 microg/g) level, respectively, were not found in the 31 dog food samples. Most of the 31 dog food samples had a PCR amplicon on PCR analysis when a PCR primer set capable of simultaneously detecting mtDNA of cows, pigs, sheep, goats, deer, elk, and horses was used. Results of PCR analysis by use of primers specific for bovine, swine, sheep and goat, or horse mtDNA revealed amplicons specific for bovine or swine mtDNA only in 27 of the 31 samples. Analysis of the remaining 4 samples failed to yield amplicons for any mammalian mtDNA. Pentobarbital was detected in 2 of these 4 samples. Results of PCR analysis correlated with the stated ingredient list for most, but not all samples. CONCLUSIONS AND CLINICAL RELEVANCE: Because canine and feline mtDNA were not found in a set of retail dog food samples, these results indicate that the source of pentobarbital in dog food is something other than proteins from rendered pet remains.  相似文献   
97.
Objective To measure the plasma cortisol response in calves dehorned by four different methods (scoop, guillotine shears, saw, embryotomy wire) for 9 h after dehorning.
Design A physiological study with controls.
Procedure Horn amputation was carried out on calves restrained manually in a race.
Results The four methods of dehorning provoked similarly increased cortisol responses which lasted for 6 h. During the first hour after dehorning the plasma cortisol concentrations were similar to those following ACTH injection. The overall cortisol response to control handling was about 30% of the responses to dehorning.
Conclusions The similarity of the cortisol responses produced by the four methods of dehorning suggests that the distress experienced by calves following dehorning by amputation is similar regardless of method used.  相似文献   
98.
99.
Bacteria of the family Chlamydiaceae are obligate intracellular pathogens of human and animals. Chlamydophila pecorum is associated with different pathological conditions in ruminants, swine and koala. To characterize a coding tandem repeat (CTR) identified at the 3' end of incA gene of C. pecorum, 51 strains of different chlamydial species were examined. The CTR were observed in 18 of 18 tested C. pecorum isolates including symptomatic and asymptomatic animals from diverse geographical origins. The CTR were also found in two strains of C. abortus respectively isolated from faeces from a healthy ewe and from a goat belonging to asymptomatic herds, but were absent in C. abortus strains isolated from clinical disease specimens, and in tested strains of C. psittaci, C. caviae, C. felis and C. trachomatis. The number of CTR repeats is variable and encode several motifs that are rich in alanine and proline. The CTR-derived variable structure of incA, which encode the Chlamydiaceae-specific type III secreted inclusion membrane protein, IncA, may be involved in the adaptation of C. pecorum to its environment by allowing it to persist in the host cell.  相似文献   
100.
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