土拉霉素在猪体内残留消除规律的研究     

倪姮佳  黄显会  贺利民  方炳虎  赵永达 《畜牧兽医学报》2011,42(4)

本研究旨在分析土拉霉素在猪体内的残留消除规律并为制定休药期提供依据。采用猪颈部一次性肌内注射土拉霉素注射液,注射剂量为2.5mg.kg-1体质量,分别在给药后第0.5,5,12,18,25,36,48天,各宰杀5头取样。样品经乙腈提取,正己烷脱脂,C18固相萃取柱(SPE)净化后用高效液相色谱—串联质谱仪分析。结果显示,给药后第0.5天注射部位药物浓度最高,第36天所有组织的药物浓度均低于最高残留限量(MRL)。用WinNonlin软件分析各组织中的消除动力学参数,消除快慢依次为注射部位,肝脏,皮脂,肌肉,肺脏和肾脏,其消除半衰期(t1/2β)分别为117.06,193.14,197.60,207.64,228.99和232.61h。肺脏的药时曲线下面积(AUC)为1 220.59μg.h.g-1仅次于注射部位及肾脏,显著高于肌肉,肝脏及皮脂。由于肾脏为代谢器官,因此可确定肺脏为土拉霉素作用的靶部位。根据欧美等国家对土拉霉素制定的最高残留限量,计算得注射部位的休药期最长,为33d。结果提示土拉霉素注射液吸收迅速,体内分布广,作用时间长。建议休药期为33d。  相似文献   

淹没线上剩余耕地质量评价及其对移民生产安置任务的影响     

刘冰  倪九派 《中国农村水利水电》2011,(9)

移民生产安置任务的确定与大型水电站的建设及移民的生产生活密切相关。以大岗山水电站淹没线上剩余的77块耕地为例,对线上剩余耕地质量评价及其对移民生产安置任务的影响进行了探讨。研究结果表明,线上剩余耕地质量主要受海拔、耕地类型、坡度和土壤类型的影响,线上剩余耕地质量评价前后移民生产安置任务分别为2 569人和3 187人。进行线上剩余耕地质量评价后,大岗山水电站淹没影响区的移民安置任务增加了24.06%。通过线上剩余耕地质量评价,能更科学合理地确定移民生产安置任务,对移民安置工作的顺利进行有重要的意义。  相似文献   

从土壤中分离获得的五氯硝基苯降解菌株降解五氯硝基苯特性研究     

LI Rong  ZHENG Jin-Wei  NI Bin  CHEN Kai  YANG Xiu-Juan  LI Shun-Peng  JIANG Jian-Dong 《土壤圈》2011,21(1):31-36

A bacterial strain,pcnb-21,capable of degrading pentachloronitrobenzene(PCNB) under aerobic and anoxic conditions,was isolated from a long-term PCNB-polluted soil by an enrichment culture technique and identified as Labrys portucalensis based upon its morphological,physiological and biochemical properties,as well as 16S rRNA gene sequence analysis.Effects of different factors,such as temperature and pH,on PCNB biodegradation were studied.Strain pcnb-21 efficiently degraded PCNB at temperatures from 20 to 30 ℃ and initial pH values from 4 to 7,which might be the first time that a Labrys strain was found capable of efficiently degrading PCNB.The degradation of PCNB was affected by oxygen,and the degradation decreased with increasing aeration.Exogenous electron donors such as glucose,lactic acid and succinic acid promoted the biodegradation of PCNB,while electron acceptors such as sodium nitrite,sodium sulfate,sodium nitrate and sodium sulfate inhibited PCNB biodegradation.The degradation of PCNB in sterile and non-sterile soils by a green fluorescent protein(GFP)-labeled strain,pcnb-21-gfp,was also studied.Cells of pcnb-21-gfp efficiently degraded 100 mg kg -1 PCNB in sterile and non-sterile soils and could not be detected after 42 days.Strain pcnb-21 might be useful in bioremediating PCNB-polluted soils and environment.  相似文献   

植被混凝土在采石场生态恢复中的应用   总被引：1，自引：1，他引：0  

唐欣  向佐湘  倪海满 《草业科学》2011,28(1):74-76

针对目前我国众多采石场面临的生态问题,以广州岑村东升采石场的生态治理为例,通过具体工程项目的实施,详细介绍了植被混凝土在采石场生态恢复治理中的施工工艺流程及应用前景,并提出了在我国南方等亚热带地区进行采石场生态恢复、岩质边坡绿化工程施工的治理思路和植物应用搭配的基本原则,从而为同类型采石场的生态恢复、混凝土或岩质边坡绿...  相似文献   

紫锥菊、黄芪对法氏囊病雏鸡的肝损伤及脾脏NK细胞活性的影响     

倪耀娣  乔健  徐建忠  刘荣欣 《中国兽医学报》2011,31(4)

给14日龄雏鸡灌服不同浓度紫锥菊、黄芪合剂,连续灌服7d,21日龄时雏鸡接种传染性法氏囊病病毒(IB-DV),来观察雏鸡血液转氨酶活性及脾脏NK细胞活性的变化。结果显示,攻毒后4d和攻毒后11d时用药组与攻毒对照组相比,谷草转氨酶(Ast)、谷丙转氨酶(Alt)均有所降低,差异显著(P<0.05);攻毒后11d时Ast/Alt比值极显著低于攻毒对照组(P<0.01)。脾脏NK细胞活性在感染后14d,高剂量组活性极显著高于攻毒对照组和空白对照组(P<0.01),感染21d后各组差异不显著。结果表明,紫锥菊、黄芪合剂在雏鸡感染IBDV后12d前后一段时间可显著降低传染性法氏囊病病毒对肝脏造成的损伤,促进脾脏NK细胞的活性,增强机体的防御功能,有利于该疾病的防治。  相似文献   

Cofilin在癌细胞迁移中的作用与调控     

原雪莹  倪华 《黑龙江动物繁殖》2011,19(3):1-5

Cofilin(丝切蛋白)是肌动蛋白解聚因子家族的重要成员,其磷酸化状态直接决定对丝状肌动蛋白的切割活性,从而在膜突的形成及细胞迁移中起到重要的作用,并且与癌症的转移密切相关.在多种癌症细胞中,Cofilin受到复杂的调控,尽管尚不能直接应用于癌症的治疗,但一些相关机制已经形成了体系,这些理论将在本文中详细阐述.  相似文献   

奶牛乳房炎金黄色葡萄球菌基因多态性分型试验     

邓海平  倪春霞  蒲万霞 《中国兽医学报》2011,31(6)

利用随机引物AP-7,建立引物随机多态性扩增(RAPD)体系对71株引起内蒙古和贵州地区奶牛乳房炎的金黄色葡萄球菌分离株进行基因分型研究。结果表明,71株金黄色葡萄球菌均得到清晰的RAPD指纹图谱,扩增产物为2～9条带,产物大小为240～4 500bp。菌株共分为6个基因型,其中Ⅰ型17株(占23.9%)、Ⅱ型3株(占4.2%)、Ⅲ型33株(占46.5%)、Ⅳ型15株(占21.1%)、Ⅴ型2株(占2.8%)、Ⅵ型2株(占2.8%)。Ⅰ型为内蒙古地区的流行优势菌群,Ⅲ型为贵州地区的流行优势菌群。两地区各基因型菌株比例有明显差异,这可能与奶牛养殖业水平和环境差异有关。  相似文献   

“经络”的物质及组织学基础研究概况     

陈军  蒙德慈  巩江  李娜  赵鹏辉  张新刚  黄龙  刘洋  倪士峰 《畜牧与饲料科学》2011,(6)

在广泛文献检索基础上,对中医系统中的重要概念——经络的物质及组织学基础研究进行了资料概述,旨在为深入研究经络实质以及中医现代化提供参考。  相似文献   

Autophagy is activated during glutamic acid-induced cortical neuron injury     

CHEN Zhao-yao  CHEN Mao-gang  LU Ting-ting  NI Guan-zhong  LIU Xin-feng  XU Ge-lin 《园艺学报》2011,27(1):62-66

AIM: To investigate whether autophagy is activated during glutamic acid-induced neuron injury and the possible neuroprotective effect of 3-methyl adenine(3-MA) (an autophagy inhibitor).METHODS: Glutamic acid or 3-MA was added to the medium of cultured cortical neurons. Cell viability was measured by MTT assay. The formation of autophagosome was observed under transmission electron microscope. The marker protein light chain 3(LC3) for autophagy was detected by immunofluorescence assay and visualized under laser confocal microscope.RESULTS: The cell viability declined during glutamic acid treatment and the autophagosomes were increased. LC3, the marker protein of autophagy, also significantly increased. The autophagy level was lowered by 3-MA, and cell viability was increased.CONCLUSION: The results suggest that autophagy is activated during glutamic acid treatment and inhibition of autophagy may have neuroprotective effect. The autophagy inhibitor 3-MA may be a potential neuroprotective agent.  相似文献   

NKX3.1 down-regulates bcl-2 gene expression in prostate cancer PC-3 cells     

CHEN Zhao-bo  LIU Chun-yan  NI Na-na  YU Yang  ZHANG Peng-ju  CHEN Wei-wen  CUI Fu-ai  JIANG An-li 《园艺学报》2011,27(10):1902-1906

AIM: To investigate the effect of NKX3.1 on the gene expression of bcl-2 and apoptosis in prostate cancer PC-3 cells. METHODS: The eukaryotic expression plasmid pcDNA3.1- NKX3.1 was transiently transfected into PC-3 cells. RT-PCR and Western blotting were used to detect the effects of NKX3.1 on the expression of bcl-2 gene. Down-regulatory effect of NKX3.1 on bcl-2 gene was detected by EMSA. Flow cytometry and apoptotic body staining were carried out to study the effects of NKX3.1 on apoptosis of PC-3 cells. RESULTS: The mRNA and protein expression of bcl-2 in PC-3 cells was down-regulated by over-expression of NKX3.1. The EMSA result showed that NKX3.1 interacted with the NKX3.1 binding elements in upstream regulatory region of bcl-2 gene. The results of flow cytometry showed that the number of apoptotic PC-3 cells increased by 1.41-fold after NKX3.1 transfection to PC-3 cells. NKX3.1 increased the apoptotic bodies stained by Hoechst 33258 significantly. CONCLUSION: NKX3.1 down-regulates the expression of anti-apoptotic gene bcl-2 and induces the apoptosis of prostate cancer PC-3 cells.  相似文献