Molecular and biological characterization of <Emphasis Type="Italic">Chrysanthemum stem necrosis virus</Emphasis> isolates from distinct regions in Japan     

Minoru?TakeshitaEmail author  Naoko?Nagai  Mitsuru?Okuda  Shohei?Matsuura  Shiori?Okuda  Naruto?Furuya  Kenichi?Tsuchiya 《European journal of plant pathology / European Foundation for Plant Pathology》2011,131(1):9-14

Three isolates of Chrysanthemum stem necrosis virus (CSNV) were obtained from chrysanthemum plants in distinct regions of Japan in 2006 and 2007. All the original host plants showed severe necrotic symptoms on the leaves and stems. Amino acid sequence data of the nucleocapsid protein genes of the three isolates (CbCh07A, TcCh07A, and GnCh07S) showed high identities with those of two other CSNV isolates, HiCh06A L1 from Japan and Chry1 from Brazil. Furthermore, for the first time the complete nucleotide sequence of the S RNA was determined for CSNV (isolate HiCh06A). In phylogenetic analysis based on the non-structural protein genes from the genus Tospovirus, HiCh06A L1 was placed in the same genetic group as Tomato spotted wilt virus (TSWV) and Impatiens necrotic spot virus. Host range examination for isolates HiCh06A L1 and CbCh07A showed that green pepper (cv. ‘Kyoyutaka’, ‘Saitamawase’, ‘Tosakatsura’, ‘L3 sarara’ and ‘L3 miogi’) and tomato (cv. ‘Sekaiichitomato’) were systemically susceptible hosts, whereas TSWV-resistant Solanaceae species, Capsicum chinense, Lycopersicon peruvianum and a TSWV-resistant cultivar of green pepper (cv. TSR miogi), were resistant.  相似文献   

Detection and quantification of protein residues in food grade amino acids and nucleic acids using a dot-blot fluorescent staining method     

Yamada N  Ozawa S  Kageyama N  Miyano H 《Journal of agricultural and food chemistry》2004,52(17):5329-5333

Food allergies represent an important health problem in industrialized countries, such that detection and quantitative analysis of the protein considered to be the main allergen is crucial. A dot-blot fluorescent staining method for the detection and quantitative analysis of protein residues in food grade amino acids and nucleic acids is presented. This method combines fluorescence staining with dot-blotting onto PVDF membrane. Several standard proteins, such as bovine serum albumin (66 kDa), lysozyme (14 kDa), ubiquitin (8.6 kDa), bovine insulin (5.7 kDa), and oxidized insulin B chain (3.5 kDa), were detectable at 0.1 ppm using SYPRO Ruby blot stain. Twenty-five different amino acids and two different nucleic acids of food grade were analyzed using this method combined with an internal standard addition method using BSA as an internal standard. All amino acids and nucleic acids were dissolved in 3.6% aqueous HCl and dot-blotted onto PVDF membrane before a large amount of amino acids and nucleic acid were removed. Protein residues and the internal standard protein immobilized on the membrane were stained using SYPRO ruby blot stain. The internal standard in all samples was detectable at 0.1 ppm. Samples were dissolved at 120 or 70 mg/mL, according to their solubility under acidic conditions. The detection limit of protein residues per weight was 0.8-1.4 ppm in amino acids and nucleic acids; residual protein was not detected in any sample.  相似文献   

Measurement of Fertilizer Leaching from the Root Zone Using an Automated Infiltration Soil Water Sampler in an Unsaturated Sandy Field     

Naoko Higashi  Yasushi Mori  Mitsuhiro Inoue 《Soil Science and Plant Nutrition》2005,51(7):1023-1033

In sandy fields with vegetable cultivation, fertilizer leaching may occur and it should be well-controlled. The development of a direct soil water sampler is necessary to examine solute transport and fertilizer leaching in the vadose zone, since soil water reflects timely monitoring of data more accurately than groundwater. We developed a Suction-Controlled Flux Sampler to collect infiltration soil water in a sandy soil. In the present study, we monitored fertilizer leaching in an unsaturated sandy field during the rainy season, while evaluating the sampling performance of SCFS for the sampling of infiltration water. SCFS directly collected the infiltration water effectively over a period of several months in the sandy field and recorded the Water-Collecting Efficiency from 92 to 115% under various infiltration conditions during a period of 50 d. WCE was affected by the rainfall intensity as well as by previous rainfall, which enhanced WCE. The results obtained from the use of SCFS and several sensors demonstrated that the amount of leached water remained low as long as irrigation was applied according to the cultivation manual. However, an unexpected heavy rainfall event led to fertilizer leaching. The fertilizer leaching trend was effectively monitored by several sensors inserted into the soils, while detailed analysis of the components was performed after collection by using SCFS. Direct access to infiltration water enabled to examine the infiltration process and detailed variations in the amounts of discharged anions. The sensor-equipped monitoring system together with SCFS is suitable for precise management of fertilizer and irrigation application.  相似文献   

Changes in expression and localization of connexin 43 mRNA and protein in porcine ovary granulosa cells during follicular atresia   总被引：2，自引：0，他引：2  

Cheng Y  Inoue N  Matsuda-Minehata F  Goto Y  Maeda A  Manabe N 《The Journal of reproduction and development》2005,51(5):627-637

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Aberrant development of mammary glands, but precocious expression of beta-casein in transgenic females ubiquitously expressing whey acidic protein transgene     

Iwamori T  Oosawa M  Nukumi N  Kano K  Sudo K  Naito K  Tojo H 《The Journal of reproduction and development》2005,51(5):579-592

It has been suggested that whey acidic protein (WAP) may function as a protease inhibitor. However, the actual function of WAP remains obscure. We investigated the histological development of the mammary glands of transgenic mice ubiquitously expressing WAP and CAG/WAP transgene. Ubiquitous expression of WAP induced aberrant development of the lobular alveoli of the mammary glands: mammary alveoli that were either aberrantly large or small in size increased in number in the developing mammary glands of these transgenic females during pregnancy and lactation. The expression of beta-casein was precociously induced in the mammary glands of the transgenic females during early pregnancy and accompanying this was a histological observation that abnormally developed lobular alveoli filled with milk proteins appeared in the mammary glands of transgenic females during early pregnancy. However, during lactation, the development of mammary glands was impaired in transgenic females. To investigate the possible paracrine action of WAP associated with mammary gland aberration, we transplanted the mammary tissue of CAG/EGFP transgenic females into the fat pad of virgin CAG/WAP transgenic females and initiated pregnancy by mating. The development of mammary tissue transplanted to the recipient was histologically examined on day 3 of lactation. The results revealed that the development of grafted mammary tissues was impaired in a manner similar to that of the mammary glands of CAG/WAP transgenic females, indicating that the inhibitory effect of WAP acts via a paracrine mechanism. In vitro experiments using HC11 cells with forced expression of exogenous WAP demonstrated the inhibitory function of WAP on proliferation of mammary epithelial cells.  相似文献   

Porcine (Sus scrofa) cellular FLICE-like inhibitory protein (cFLIP): molecular cloning and comparison with the human and murine cFLIP     

Goto Y  Matsuda-Minehata F  Inoue N  Matsui T  Maeda A  Manabe N 《The Journal of reproduction and development》2004,50(5):549-555

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The regulation of ovarian granulosa cell death by pro- and anti-apoptotic molecules   总被引：1，自引：0，他引：1  

Matsuda-Minehata F  Inoue N  Goto Y  Manabe N 《The Journal of reproduction and development》2006,52(6):695-705

In the mammalian ovary, follicular development and atresia are closely regulated by cell death and survival-promoting factors, including hormones (gonadotropins) and intraovarian regulators (gonadal steroids, cytokines, and intracellular proteins). Several hundred thousand primordial follicles are present in the mammalian ovary; however, only a limited number of primordial follicles develop to the preovulatory stage and ovulate. The others, more than 99% of follicles, will be eliminated via a degenerative process known as "atresia". The endocrinological regulatory mechanisms involved in follicular development and atresia have been characterized to a large extent, but the precise temporal and molecular mechanisms involved in the regulation of these events have remained largely unknown. Recent studies suggest that the apoptosis of ovarian granulosa cells plays a major role in follicular atresia. In this review, we provide an overview of development and atresia of follicles, and apoptosis of granulosa cells in mammals.  相似文献   

Expression and localization of Fas ligand and Fas during atresia in porcine ovarian follicles     

Inoue N  Maeda A  Matsuda-Minehata F  Fukuta K  Manabe N 《The Journal of reproduction and development》2006,52(6):723-730

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The influence of age and body mass index on relative accuracy of energy intake among Japanese adults     

Okubo H  Sasaki S  Hirota N  Notsu A  Todoriki H  Miura A  Fukui M  Date C 《Public health nutrition》2006,9(5):651-657

OBJECTIVE: To examine relationships between the ratio of energy intake to basal metabolic rate (EI/BMR) and age and body mass index (BMI) among Japanese adults. DESIGN: Energy intake was assessed by 4-day semi-weighed diet records in each of four seasons (16 days in total). The EI/BMR ratio was calculated from reported energy intake and estimated basal metabolic rate as an indicator of reporting accuracy. SETTING: Residents in three areas in Japan, namely Osaka (urban), Nagano (rural inland) and Tottori (rural coastal). SUBJECTS: One hundred and eighty-three healthy Japanese men and women aged > or =30 years. RESULTS: The oldest age group (> or =60 years) had higher EI/BMR values than the youngest age group (30-39 years) in both sexes (1.74 vs. 1.37 for men; 1.65 vs. 1.43 for women). In multiple regression analyses, age correlated positively (partial correlation coefficient, beta = 0.012, P < 0.001 for men; beta = 0.011, P < 0.001 for women) and BMI correlated negatively (beta = -0.031, P < 0.001 for men; beta = -0.025, P < 0.01 for women) with EI/BMR. CONCLUSION: Age and BMI may influence the relative accuracy of energy intake among Japanese adults.  相似文献   

Innate lymphoid cells promote anatomical containment of lymphoid-resident commensal bacteria     

Sonnenberg GF  Monticelli LA  Alenghat T  Fung TC  Hutnick NA  Kunisawa J  Shibata N  Grunberg S  Sinha R  Zahm AM  Tardif MR  Sathaliyawala T  Kubota M  Farber DL  Collman RG  Shaked A  Fouser LA  Weiner DB  Tessier PA  Friedman JR  Kiyono H  Bushman FD  Chang KM  Artis D 《Science (New York, N.Y.)》2012,336(6086):1321-1325

The mammalian intestinal tract is colonized by trillions of beneficial commensal bacteria that are anatomically restricted to specific niches. However, the mechanisms that regulate anatomical containment remain unclear. Here, we show that interleukin-22 (IL-22)-producing innate lymphoid cells (ILCs) are present in intestinal tissues of healthy mammals. Depletion of ILCs resulted in peripheral dissemination of commensal bacteria and systemic inflammation, which was prevented by administration of IL-22. Disseminating bacteria were identified as Alcaligenes species originating from host lymphoid tissues. Alcaligenes was sufficient to promote systemic inflammation after ILC depletion in mice, and Alcaligenes-specific systemic immune responses were associated with Crohn's disease and progressive hepatitis C virus infection in patients. Collectively, these data indicate that ILCs regulate selective containment of lymphoid-resident bacteria to prevent systemic inflammation associated with chronic diseases.  相似文献