Binding of mammalian and avian ferritins with biotinylated hemin: demonstration of preferential binding of the H subunit to heme     

Nakai M  Murata N  Yoda T  Yoshikawa Y  Watanabe K  Orino K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(3):313-318

The binding of ferritin to heme has been well studied using commercial horse spleen apoferritin, which is almost entirely composed of the L subunit, suggesting that mammalian ferritins bind heme. The present study revealed that both mammalian holoferritins (commercial horse spleen ferritin and purified horse spleen, bovine spleen and canine liver ferritins with L/H subunit ratios of 4.0, 1.1, and 2.3, respectively) and their apoferritins bound biotinylated hemin; apoferritins had higher binding activity than holoferritins, except for canine holo- and apoferritins, which showed the same binding. Bovine ferritin H subunit homopolymers expressed by a baculovirus expression system showed heme binding and had higher binding activity to biotinylated hemin than the L subunit homopolymer expressed by the same system. These bindings were inhibited by heme but not by iron-free or Zn-protoporphyrin IX (Zn-PPIX). Purified chicken liver holoferritin was found to be composed of only H subunits and showed the highest binding activity with biotinylated hemin compared with mammalian holoferritins. The binding of chicken liver holoferritin to biotinylated hemin was also inhibited by heme but not by PPIX or Zn-PPIX. These results indicate that mammalian and avian ferritins bind heme and that the H subunit preferentially recognizes heme.  相似文献   

Knockout of targeted gene in porcine somatic cells using zinc‐finger nuclease          下载免费PDF全文

Shin Hisamatsu  Motoharu Sakaue  Akiko Takizawa  Tsubasa Kato  Maki Kamoshita  Junya Ito  Naomi Kashiwazaki 《Animal Science Journal》2015,86(2):132-137

Targeted genome editing is a widely applicable approach for efficiently modifying any sequence of interest in animals. It is very difficult to generate knock‐out and knock‐in animals except for mice up to now. Very recently, a method of genome editing using zinc‐finger nucleases (ZFNs) has been developed to produce knockout rats. Since only injection of ZFNs into the pronuclear (PN) embryo is required, it seems to be useful for generating gene‐targeted animals, including domestic species. However, no one has reported the successful production of knockout pigs by direct injection of ZFNs into PN embryos. We examined whether ZFN works on editing the genome of porcine growth hormone receptor in two kinds of cell lines (ST and PT‐K75) derived from the pig as a preliminary study. Our data showed that pZFN1/2 vectors were efficiently transfected into both ST and PT‐K75 cells. In both cell lines, results from Cel‐I assay showed that modification of the targeted gene was confirmed. We injected ZFN1/2 mRNAs into the nucleus of PN stage embryos and then they were transferred to the recipients. However, pups were not delivered. Taken together, ZFN can be an available technology of genome editing even in the pig but further improvement will be required for generating genome‐modified pigs.  相似文献   

Ecology. New count of old whales adds up to big debate     

Lubick N 《Science (New York, N.Y.)》2003,301(5632):451

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澳大利亚的杜鹃及其寄生适应性(英文)     

Naomi E.LANGMORE 《中国鸟类》2013,4(1):86-92

长期以来,人们一直对寄生性繁殖的杜鹃所采用的种种计谋惊叹不已。本文对澳大利亚的寄生杜鹃如何增加寄生巢的可获得性、如何寻找和接近巢以及如何让宿主接受它们的寄生卵与雏鸟进行了综述。这些杜鹃所采用的对策,有一些是世界上其他杜鹃种类也采用的,但有一些对策,如产隐蔽的卵和模拟的雏鸟,却似乎仅见于澳大利亚的杜鹃。泛性寄生的杜鹃因为要应对较多种类的宿主,难度自然远大于专一性寄生的杜鹃种类,这同时也解释了为什么不同种类的杜鹃进化出不同的寄生策略,以避免不同宿主的识别。  相似文献   

Cardiovascular Effects of Dobutamine and Phenylephrine Infusion in Sevoflurane-anesthetized Thoroughbred Horses     

Minoru OHTA  Shinjiro KURIMOTO  Yuhiro ISHIKAWA  Hirotaka TOKUSHIGE  Naomi MAE  Shun-ichi NAGATA  Masayuki MAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(11):1443-1448

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Vitrification procedure decreases inositol 1,4,5‐trisphophate receptor expression,resulting in low fertility of pig oocytes     

Masahiko Hirose  Maki Kamoshita  Katsuyoshi Fujiwara  Tsubasa Kato  Ayaka Nakamura  Richard J. H. Wojcikiewicz  Jan B. Parys  Junya Ito  Naomi Kashiwazaki 《Animal Science Journal》2013,84(10):693-701

Although cryopreservation of mammalian oocytes is an important technology, it is well known that unfertilized oocytes, especially in pigs, are highly sensitive to low temperature and that cryopreserved oocytes show low fertility and developmental ability. The aim of the present study was to clarify why porcine in vitro matured (IVM) oocytes at the metaphase II (MII) stage showed low fertility and developmental ability after vitrification. In vitro matured cumulus oocyte complexes (COCs) were vitrified with Cryotop and then evaluated for fertility through in vitro fertilization (IVF). Although sperm‐penetrated oocytes were observed to some extent (30–40%), the rate of pronuclear formation was low (9%) and none of them progressed to the two‐cell stage. The results suggest that activation ability of cryopreserved oocytes was decreased by vitrification. We examined the localization and expression level of the type 1 inositol 1,4,5 trisphosphate receptor (IP₃R1), the channel responsible for Ca²⁺ release during IVF in porcine oocytes. Localization of IP₃R1 close to the plasma membrane and total expression level of IP₃R1 protein were both decreased by vitrification. In conclusion, our present study indicates that vitrified‐warmed porcine COCs showed a high survival rate but low fertility after IVF. This low fertility seems to be due to the decrease in IP₃R1 by the vitrification procedure.  相似文献   

Post harvest treatments to control eggplant deterioration during storage     

Naomi Temkin-Gorodeiski  B. Shapiro  Shoshana Grinberg  Ida Rosenberger  E. Fallik 《The Journal of Horticultural Science and Biotechnology》2013,88(5):689-693

Eggplant (Solatium melongena L.) fruits deteriorate rapidly during prolonged storage, mainly due to accelerated senescence of the calyx. Dipping the calyx in a solution containing 200 ppm NAA and 900 ppm prochloraz (active ingredient) retarded calyx senescence and controlled decay. At the end of the growing season in Israel (March-May), dipping the whole fruit in the above solution gave better results than dipping the calyx only. After 14 d of storage at 12°C and 3 d at 20°C, the calyx appeared fresh and green and the fruit was firm. Decay incidence did not exceed 5%. The residual level of prochloraz in fruits that were wholly dipped was less than 0.34 mg 1“'.  相似文献   

The Morphophysiological Responses of Free-Floating Aquatic Macrophytes to a Supra-optimal Supply of Manganese     

Claudineia Lizieri  Kacilda Naomi Kuki  Rosane Aguiar 《Water, air, and soil pollution》2012,223(5):2807-2820

Among the many anthropogenic abiotic stresses, manganese (Mn) toxicity has been recognized for its impact on aquatic ecosystems as well as on the biological components of these ecosystems, including aquatic plants. The objective of this study was to determine the Mn accumulation ability of aquatic macrophytes (Azolla caroliniana, Salvinia minima and Spirodela polyrhiza) and evaluate the morphophysiological responses of the species that gather the highest amount of Mn when exposed to a supra-optimal supply of manganese. The experiments were conducted in the laboratory, and the effects of Mn were evaluated based on plant growth; the concentration of total chlorophyll, carotenoids, and anthocyanins; the enzymatic activity of catalase and peroxidase; and leaf anatomy. All of the studied species accumulated Mn in their tissues. Moreover, it was observed that this accumulation was dependent on the concentration of the metal in solution. S. polyrhiza showed higher concentrations of Mn in its tissues (17.062?mg?g^?1 dry weight (DW)), followed by S. minima (4.283?mg?g^?1 DW) and A. caroliniana (1.341?mg?g^?1 DW). Despite the Mn accumulation in all species, S. polyrhiza was the only one selected for further analyses because of its greater ability to accumulate Mn. The high Mn concentration found in tissues of S. polyrhiza suggests that this species has the potential to sequester and accumulate this metal. However, a sensitive response in the plants exposed to higher Mn concentrations (0.4?mM) was observed. The phytotoxicity effects of this accumulation were responsible for a decrease in the plant growth, a reduction in the pigment content (total chlorophyll, carotenoids, and anthocyanins), a low activity of catalase, and the disarrangement of the leaf aerenchyma.  相似文献   

Evaluation of a semi‐controlled test as a selection tool for frost tolerance in durum wheat (Triticum durum)     

Alisa‐Naomi Sieber  Tobias Würschum  Carl Friedrich Horst Longin 《Plant Breeding》2014,133(4):465-469

Most durum wheat (Triticum durum) varieties possess only low winter hardiness due to their frost susceptibility. In North America and Central Europe, durum wheat is therefore typically sown in spring to circumvent the local winter conditions. However, the yield potential of durum in these regions could be much better exploited if durum varieties with increased frost tolerance were available, which could be sown in autumn. A factor limiting breeding for increased frost tolerance is the variation in the occurrence of frost stress across years. The ‘Weihenstephaner Auswinterungsanlage’ is a semi‐controlled test that exposes the plants to all weather conditions. Snow coverage of the plants, serving as frost protection, is prevented by the movable glass lid of the semi‐controlled test. In this study, different scorings for frost tolerance based on this semi‐controlled test were evaluated and compared with frost tolerance data in the field. Our results illustrate the potential of the ‘Weihenstephaner Auswinterungsanlage’ as an indirect selection tool for frost tolerance in durum breeding programmes, especially when regular frost tolerance data from the field are not available.  相似文献   

Successful blastocyst production by intracytoplasmic injection of sperm after in vitro maturation of follicular oocytes obtained from immature female squirrel monkeys (Saimiri boliviensis)     

Shinichi WATANABE  Megumi MIURA  Hiromi MORITA  Moeka NISHI  Shin-ichi YOKOTA  Shosaku HATTORI  Hiromichi MATSUMOTO  Emiko FUKUI  Ken Takeshi KUSAKABE  Masanori OCHI  Naomi NAKAGATA  Yasuo KISO  Chieko KAI  Midori YOSHIZAWA 《The Journal of reproduction and development》2021,67(4):265

Advanced reproductive technologies are being applied for the propagation of squirrel monkeys, to ensure their preservation as a genetic resource and the effective use of their gametes in the future. In the present study, oocytes and spermatozoa were collected from live squirrel monkeys, following which piezo intracytoplasmic sperm injection (ICSI) was performed using these gametes. Follicular development was induced by administering equine chorionic gonadotropin (eCG) containing inhibin antiserum to an immature squirrel monkey female. The unilateral ovary was excised after the administration of human chorionic gonadotropin (hCG), to induce ovulation, following which the larger developed follicular oocytes were collected. Follicular oocytes were prepared for ICSI using sperm from the epididymal tail of a unilateral testis extracted from a mature male. The embryos were continuously incubated in CMRL 1066 medium supplemented with 10% (v/v) fetal bovine serum. Embryo culture was performed with cumulus cells. Two experiments of ICSI carried out with three females resulted in 14 mature oocytes from the 49 cumulus-oocyte complexes collected and five embryos, three of which developed into blastocysts. These blastocysts were vitrified, thawed, and transferred to recipient monkeys, but no pregnancies resulted. In conclusion, the present study is the first to successfully produce ICSI-derived blastocysts from MII oocytes obtained by means of hormone administration (a combination of eCG+inhibin antiserum and hCG) and in vitro maturation in immature squirrel monkeys.  相似文献