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91.
Inoculation of Lupinus luteus with White Root Rot Fungus, Rosellinia necatrix, to Estimate Virulence
Yukari UETAKE Hitoshi NAKAMURA Masao ARAKAWA Ikuko OKABE Naoyuki MATSUMOTO 《Journal of General Plant Pathology》2001,67(4):285-287
An inoculation method using Lupinus luteus was developed for estimating virulence of isolates of the white root rot fungus, Rosellinia necatrix Prillieux. Fungal cultures grown on pieces of mulberry twigs were placed in contact with the hypocotyl of 3-week-old seedlings
growing in pots of soil. Disease development was uniform and reproducible in repeated experiments. Of 24 isolates with double-stranded
RNA, eight were weakly virulent. This method is useful throughout the year for estimating virulence of many isolates of the
fungus and for screening for hypovirulent isolates.
Received 2 March 2001/ Accepted in revised form 25 May 2001 相似文献
92.
Structural differences between myofibrillar protein,paratropomyosin, and tropomyosin as revealed by high‐performance liquid chromatography 下载免费PDF全文
Yuriko Nishio Yuichi Ushimura Shuji Ueda Naoyuki Maeda Akihito Hattori Minoru Yamanoue 《Animal Science Journal》2018,89(8):1161-1168
Paratropomyosin (PTM) composes myofibril functions to weaken the rigor linkages formed between actin and myosin during postmortem aging of muscles. PTM has the similar physico‐chemical properties as tropomyosin (TM) that is a regulatory protein of myofibrils. So far, it is unclear whether PTM is definitely different from TM, because the primary structure of PTM has not been determined yet. The aim of this study was to clarify structural difference of PTM from TM. PTM was prepared by column chromatography immediately after slaughter from broiler breast muscle, and purified by high‐performance liquid chromatography (HPLC). Purified PTM was successfully separated from TM, and the recovered PTM molecule was reduced with dithiothreitol to separate again by HPLC. Two subunits were obtained and peptides from each digested subunit by V8 protease were recovered by HPLC, and then amino acid sequences of the peptides were analyzed by protein sequencing. As a result, some amino acid residues were replaced from that of TMα1 isoform which is the major isoform of TM, and also was different between the two subunits. Therefore, it is concluded that PTM clearly differs from TM and it is suggested that functional difference in PTM from TM is attributed to amino acid replacements in subunits composing PTM. 相似文献
93.
Fujita M Takaishi Y Nagae H Watanabe N Hasegawa D Taniguchi A Orima H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):687-690
Magnetic resonance (MR) and computed tomography (CT) were performed in an 8-year-old, spayed female cat with chronic effort respiration at the inspiration phase and stertor. Increased bone opacity in the areas of the head, neck and thorax were observed on radiography. MR images showed no signal intensity on both transverse T1WI and T2WI of the nasal cavity. CT revealed increased bone density and hypertrophy of the nasal turbinate and a narrowed nasal passage. From these results, we concluded this case had osteopetrosis-like disease, and that the respiratory distress was caused by hypertrophy of the nasal turbinate. 相似文献
94.
Seishiro CHIKAZAWA Yasutomo HORI Fumio HOSHI Kazutaka KANAI Naoyuki ITO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):843-846
In the present study, we examined the relationship between serum ferritin concentration
before treatment and survival time in dogs with multicentric lymphoma. Eighteen dogs with
multicentric lymphoma were enrolled in the study. When the dogs were classified into high
and low ferritin groups on the basis of their serum ferritin concentration (3,000
ng/ml cut-off value), the median survival time of dogs
with high concentrations (≥3,000 ng/ml, n=7) was 40
days, whereas it was 360 days among dogs with low concentrations (<3,000
ng/ml, n=11). This difference was statistically
significant (P=0.001). This finding suggests that the initial high level
of serum ferritin indicates short survival time in dogs with multicentric lymphoma.
Large-scale research is necessary to confirm this finding. 相似文献
95.
Go SUGAHARA Ichiro NAITO Yuichi MIYAGAWA Takaaki KOMIYAMA Naoyuki TAKEMURA Ryosuke KOBAYASHI Takayuki MINESHIGE Junichi KAMIIE Kinji SHIROTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1175-1178
The renal biopsy tissue from a 9-month-old, male Pyrenean Mountain dog with
renal disorder and severe proteinuria was examined. Ultrastructural examination revealed
multilaminar splitting and fragmentation of the glomerular basement membrane (GBM) and
diffuse podocyte foot process effacement. Immunofluorescent staining for α(IV) chains
revealed presence of α5(IV) and complete absence of α3(IV) and α4(IV) chains in the GBM.
Immunohistochemistry also revealed decreased and altered expression of nephrin and podocin
in the glomeruli compared with normal canine glomeruli. These results suggested that the
glomerular disease of the present case might be consistent with canine hereditary
nephropathy resembling human Alport syndrome caused by genetic defect of type IV collagen,
and indicated possible contribution of podocyte injury to severe proteinuria in this
case. 相似文献
96.
Abdul Razaq IRSHAD Taihei SASAKI Tomoaki KUBO Naoyuki ODASHIMA Keiji KATANO Takeshi OSAWA Toru TAKAHASHI Yoshiaki IZAIKE 《The Journal of reproduction and development》2015,61(5):485-488
The objectives of the present study were to develop a programmable piggyback syringe pump for bovine superovulation and to evaluate the effects of a four-times-a-day injection regimen using the pump. Non-lactating Holstein cows were treated with a total of 30 armour units of porcine FSH by injection four times a day with the pump (study, n = 9) or injection twice a day manually (control, n = 9) for four consecutive days from D10 of the estrous cycle. The pump-driven program successfully induced superovulation in all cows tested. The numbers of small (3– < 5 mm in diameter) and large (≥ 10 mm in diameter) follicles were greater in the study group on D11-13 and D14, respectively. There were fewer unovulated follicles detected on D21 (7 days after estrus) in the study group than in the control group (1.2 ± 0.4 and 3.2 ± 0.6, respectively). 相似文献
97.
Shoma MIKAWA Yuichi MIYAGAWA Noriko TODA Yoshinori TOMINAGA Naoyuki TAKEMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):7-13
Pulmonary hypertension (PH) often occurs due to a left heart disease, such as
myxomatous mitral valve disease (MMVD), in dogs and is diagnosed using Doppler
echocardiography and estimated pulmonary arterial pressure. Diagnosis of PH in dogs
requires expertise in echocardiography: however, the examination for PH is difficult to
perform in a clinical setting. Thus, simple and reliable methods are required for the
diagnosis of PH in dogs. The purpose of this study was to develop models using multiple
logistic regression analysis to detect PH due to left heart disease in dogs with MMVD
without echocardiography. The medical records of dogs with MMVD were retrospectively
reviewed, and 81 dogs were included in this study and classified into PH and non-PH
groups. Bivariate analysis was performed to compare all parameters between the groups, and
variables with P values of <0.25 in bivariate analysis were included
in multiple logistic regression analysis to develop models for the detection of PH. In
multiple logistic regression analysis, the model included a vertebral heart scale short
axis of >5.2 v, and a length of sternal contact of >3.3 v was considered suitable
for the detection of PH. The predictive accuracy of this model (85.9%) was judged
statistically adequate, and therefore, this model may be useful to screen for PH due to
left heart disease in dogs with MMVD without echocardiography. 相似文献
98.
Katashi Kubo Naoyuki Kawada Takashi Nakajima Kazuyuki Hirayae Masaya Fujita 《Euphytica》2014,200(1):81-93
Fusarium head blight (FHB) remains a serious problem that causes yield and grain quality losses, and mycotoxin accumulation in wheat production in western Japan. A 3-year field trial with artificial FHB inoculation was conducted to evaluate varietal characteristics of FHB resistance among 31 wheat cultivars/lines cultivated in western Japan, including one standard line. Severity of FHB, frequency of Fusarium-damaged kernels (FDK), deoxynivalenol concentration (DON), nivalenol concentration (NIV), and grain yield showed significant differences among years and among cultivars/lines. Interaction between years and cultivars/lines was also significant in these traits, but F values were larger for cultivars/lines than for the interaction. Correlation analysis showed that cultivars/lines with lower FHB severities tended to have lower FDK, DON and NIV, and a higher yield. Resistance to kernel infection (RKI), residuals calculated by regressing FDK against FHB severity, and resistance to mycotoxin accumulation (RTA), residuals calculated by regressing DON + NIV against FDK, also differed significantly among cultivars/lines. These results indicated that varietal differences in response to FHB symptom development, RKI and RTA exist among wheat cultivars/lines in western Japan. Such information is important to aid producers in controlling the disease and for breeders to improve FHB resistance and reduce mycotoxin accumulation in commercial wheat cultivars. 相似文献
99.
Hiroaki Maeda Takuya Yamaguchi Motoyasu Omoteno Takeshi Takarada Kenji Fujita Kazumasa Murata Yukihide Iyama Yoichiro Kojima Makiko Morikawa Hidenobu Ozaki Naoyuki Mukaino Yoshinori Kidani Takeshi Ebitani 《Breeding Science》2014,64(2):134-141
Rice (Oryza sativa L.) can produce black grains as well as white. In black rice, the pericarp of the grain accumulates anthocyanin, which has antioxidant activity and is beneficial to human health. We developed a black rice introgression line in the genetic background of Oryza sativa L. ‘Koshihikari’, which is a leading variety in Japan. We used Oryza sativa L. ‘Hong Xie Nuo’ as the donor parent and backcrossed with ‘Koshihikari’ four times, resulting in a near isogenic line (NIL) for black grains. A whole genome survey of the introgression line using DNA markers suggested that three regions, on chromosomes 1, 3 and 4 are associated with black pigmentation. The locus on chromosome 3 has not been identified previously. A mapping analysis with 546 F2 plants derived from a cross between the black rice NIL and ‘Koshihikari’ was evaluated. The results indicated that all three loci are essential for black pigmentation. We named these loci Kala1, Kala3 and Kala4. The black rice NIL was evaluated for eating quality and general agronomic traits. The eating quality was greatly superior to that of ‘Okunomurasaki’, an existing black rice variety. The isogenicity of the black rice NIL to ‘Koshihikari’ was very high. 相似文献
100.
Acacia plantation establishment might cause soil acidification in strongly weathered soils in the wet tropics because the base cations in the soil are translocated rapidly to plant biomass during Acacia growth. We examined whether soils under an Acacia plantation were acidified, as well as the factors causing soil acidification. We compared soils from 10 stands of 8-year-old Acacia mangium plantations with soils from 10 secondary forests and eight Imperata cylindrica grasslands, which were transformed into Acacia plantations. Soil samples were collected every 5–30 cm in depth, and pH and related soil properties were analyzed. Soil pH was significantly lower in Acacia plantations and secondary forests than in Imperata grasslands at every soil depth. The difference was about 1.0 pH unit at 0–5 cm and 0.5 pH unit at 25–30 cm. A significant positive correlation between pH and base saturation at 0–20 cm depth indicated that the low pH under forest vegetation was associated with exchangeable cation status. Using analysis of covariance (ANCOVA), with clay content as the covariate, exchangeable Ca (Ex-Ca) and Mg (Ex-Mg) stocks were significantly lower in forested areas than in Imperata grasslands at any clay content which was strongly related to exchangeable cation stock. The adjusted average Ex-Ca stock calculated by ANCOVA was 249 kg ha−1 in Acacia plantations, 200 kg ha−1 in secondary forests, and 756 kg ha−1 in Imperata grasslands at 0–30 cm. Based on a comparison of estimated nutrient stocks in biomass and soil among the vegetation types, the translocation of base cations from soil to plant biomass might cause a decrease in exchangeable cations and soil acidification in Acacia plantations. 相似文献