Random amplification of polymorphic DNA and phenotypic typing of Zimbabwean isolates of Pasteurella multocida     

Dziva F  Christensen H  Olsen JE  Mohan K 《Veterinary microbiology》2001,82(4):361-372

Eighty-one isolates presumptively identified as Pasteurella multocida from a variety of diseases in animals in Zimbabwe were subjected to biochemical characterization, capsular typing and RAPD analysis. The majority of isolates (over 80%) were assigned into named taxa and were predominantly P. multocida subsp. multocida and P. multocida subsp. septica, whilst the remainder were unassigned. Serogroup A was predominant among the three capsular types (A, B and D) of P. multocida detected. Three main RAPD clusters and three subclusters were observed among the majority of isolates (93.8%), whilst the remainder was found to be weakly related. Nine different groups of strains with similar RAPD profiles (100% similarity) were also observed. The reference strain of capsular serogroup F clustered with the reference strain of P. multocida subsp. septica, whilst all other serogroups clustered with reference strains of subsp. multocida and gallicida. Notably, serogroups A and D were observed to be closely related to the reference strain of subsp. multocida. The relationship between biotype, capsular type, host origin and disease manifestation was not clear-cut. However, most pig isolates of subsp. multocida clustered together as did most cattle isolates of subsp. multocida. RAPD tended to separate subsp. multocida from septica.  相似文献   

In vitro clonal propagation of Gardenia latifolia Ait.: a toy making woody tree     

Y. Mohan Reddy  K. V. Saritha 《Agroforestry Systems》2013,87(3):591-598

An efficient, in vitro clonal propagation protocol has been established for Gardenia latifolia Ait. using mature nodal explants on Murashige and Skoog (MS) medium fortified with cytokinins (BA/Kn/2-iP) (1.0–5.0 mg l^?1) in combination with auxin IAA (0.5 mg l^?1). Maximum bud break (87 %) with shoot number (7.2 ± 0.26) observed on MS medium supplemented with BA (4.0 mg l^?1) and IAA (0.5 mg l^?1). Maximum number of shoots (30 ± 0.46) with shoot length of (0.9 ± 0.03 cm) observed on MS medium supplemented with BA (2.0 mg l^?1), Kn (2.0 mg l^?1) and IAA (0.5 mg l^?1). Further elongation of shoots (3.5 ± 0.06 cm) was achieved on MS medium supplemented with BA (1.0 mg l^?1) and IAA (0.1 mg l^?1). About 70 % of root induction occurred in half-strength MS medium supplemented with IBA (4.0 mg l^?1) in 4–6 weeks. Further elongation of roots with average length (9.0 cm) was achieved in culture bottles containing vermiculite and ¼ strength MS salts. After their partial hardening in these bottles for 30 days they were transferred to pots containing a mixture of soil and vermicompost (1:1) for acclimatization. The acclimatized plantlets were established in the field successfully with 85 % survival rate.  相似文献   

Detection and differentiation of pigeon paramyxovirus serotype-1 (PPMV-1) isolates by RT-PCR and restriction enzyme analysis     

Kuttanda A. Naveen  Shambhu Dayal Singh  Jag Mohan Kataria  Rajamani Barathidasan  Kuldeep Dhama 《Tropical animal health and production》2013,45(5):1231-1236

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Surgical Correction of Uterine Torsion and Mare–Foal Survival in Advance Pregnant Equine Patients     

Narinder S. Saini  Jitender MohindrooShashi K. Mahajan  PhD  Mulinti RaghunathAshwani Kumar  PhD  Vandana SangwanTarunbir Singh  PhD  Navdeep SinghSimrat S. Singh  DVM  PhD  Arun AnandKiranjeet Singh  PhD 《Journal of Equine Veterinary Science》2013

This article describes the surgical management of uterine torsion by midline celiotomy and cesarean section on 12 mares presented with signs of colic to a teaching veterinary hospital. The mares were either in full term of gestation (n = 7) or in advanced stage of pregnancy (n = 5). Six mares were in first parity. Uterine torsion was diagnosed by per rectal and per vaginal examinations. For surgical intervention, mares were anesthetized using a combination of xylazine (1.1 mg/kg) and ketamine (2.2 mg/kg), intravenously. After intubation, the animals were maintained on halothane (n = 4) or isoflurane (n = 8) inhalation anesthesia. Midline celiotomy was performed, and foals were delivered by cesarean section. In 11 mares, before closing the abdominal wound, the uterus was detorted manually and confirmed for its normal position. Both anesthetic protocols using halothane and isoflurane were found satisfactory for surgical correction of uterine torsion. After long-term follow-up, the study reported 75.0% (9/12) survival rate for mares. One mare was euthanized because of devitalized, necrosed, and adhered uterus to the abdominal wall. Of the nine surviving mares, seven were successfully bred. Three foals were born alive, and only one could survive on long-term basis. Of the nine dead foals, two had umbilical cord torsion.  相似文献   

A Comprehensive Pathological Survey of Duodenal Biopsies from Dogs with Diet‐Responsive Chronic Enteropathy     

Y‐M. Chang  C. Downes  S. Miller  K. Stevens  K. Verheyen  A.D. Phillips  S. Miah  M. Turmaine  A. Hibbert  J.M. Steiner  J.S. Suchodolski  K. Mohan  J. Eastwood  K. Allenspach  K. Smith  O.A. Garden 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2013,27(4):862-874

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Expression profile of toll like receptors in a range of water buffalo tissues (Bubalus bubalis)     

Vahanan BM  Raj GD  Pawar RM  Gopinath VP  Raja A  Thangavelu A 《Veterinary immunology and immunopathology》2008,126(1-2):149-155

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Optical coherence tomography and molecular analysis of sudden acquired retinal degeneration syndrome (SARDS) eyes suggests the immune‐mediated nature of retinal damage     

Sinisa D. Grozdanic  Tatjana Lazic  Helga Kecova  Kabhilan Mohan  Markus H. Kuehn 《Veterinary ophthalmology》2019,22(3):305-327

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Morpho-Physiological Responses of Grape Rootstock ‘Dogridge’ to Arbuscular Mycorrhizal Fungi Inoculation Under Salinity Stress     

Kaushal Kishore Upreti  Ravindra Mohan Bhatt  Periyasamy Panneerselvam  Lakki Reddy Varalakshmi 《International Journal of Fruit Science》2016,16(2):191-209

Effects of arbuscular mycorrhizal fungi (AMF) alone or in combination with bacterial consortium (AMF+BC) inoculation prior to induced salinity (NaCl @ 150 or 250 mM) were studied on root growth; plant biomass; leaf area; Na⁺ and K⁺ contents; leaf water potential (Ψ_w); osmotic potential (Ψ_π); photosynthesis rate (P_n); and contents of chlorophyll, phytohormones, and polyamines in the grape rootstock ‘Dogridge’, popular among Indian vine growers. AMF inoculation in the NaCl untreated rootstocks plants increased root growth, root and shoot biomass, and leaf area and improved leaf Ψ_w, Ψ_π, P_n, and chlorophyll content, and also countered the stress-induced decline in the NaCl treated plants. The abscisic acid (ABA), cytokinins, and polyamine-spermidine and spermine contents in the leaves of NaCl untreated or treated were significantly increased by the AMF inoculation. Among the treatments, AMF with BC was relatively more effective than AMF alone with respect to changes in above morpho-physiological characters. The results depicted that AMF (AMF alone or AMF+BC) inoculation significantly improved salinity tolerance of grape rootstock and tolerance is induced by improvements in plant water balance, K⁺:Na⁺ ratio, and P_n, besides distinct accumulations in ABA and polyamines-spermine and spermidine. The above findings have potential in suggesting the AMF usefulness in improving the efficacy of ‘Dogridge’ rootstock in grape cultivation under salt affected soils.  相似文献   

Enhancing Growth of Common Carp, Rohu and Mozambique Tilapia through Plant Substrate: The Role of Bacterial Biofilm   总被引：1，自引：0，他引：1  

N.R. Umesh  K.M. Shankar  C.V. Mohan 《Aquaculture International》1999,7(4):251-260

Experiments were conducted to enhance the growth of common carp (Cyprinus carpio), rohu (Labeo rohita) and Mozambique tilapia (Oreochromis mossambicus) through use of sugarcane bagasse as substrate. Bagasse was suspended in water with or without supplementation with fertilizers. Bagasse supplemented with cattle dung and urea favoured higher zooplankton production and significantly (p < 0.05) increased fish growth by over 50% compared to bagasse or fertilizers on their own. This higher production of fish is attributed to bacterial biofilm promoted on the substrate which, apart from forming food for zooplankton and fish, contributed to improved water quality by lowering ammonia.  相似文献   

Characterization of Spray-Dried Hydrolyzed Proteins from Pink Perch Meat Added with Maltodextrin and Gum Arabic     

Lakshmi Narasimha Murthy  Girija Gajanan Phadke  Chitradurga Obaiah Mohan  Madhamuthanalli Venkataramappa Chandra  Jeyakumari Annamalai  Sivam Visnuvinayagam 《Journal Of Aquatic Food Product Technology》2017,26(8):913-928

In the present study, spray-dried pink perch meat protein hydrolysate (PPH) and hydrolysate with maltodextrin and gum arabic (PPHMG) were characterized in relation to their physical, rheological, functional, antioxidant, thermal, and sensory properties. The Herschel–Bulkley model was suitable to explain the flow behavior of PPH and PPHMG emulsions. Both the samples exhibited functional and antioxidant properties. Antioxidant activities were significantly higher for PPH compared with PPHMG (p<0.05). PPH and PPHMG alone did not exhibit antimicrobial activity but enhanced the activity of chitosan against pathogens. Sensory acceptability of the samples in soup revealed that PPHMG of up to 4% was highly scored without any traces of bitterness, whereas for PPH, a concentration of up to 3% was acceptable.  相似文献