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Kobori M Nakayama H Fukushima K Ohnishi-Kameyama M Ono H Fukushima T Akimoto Y Masumoto S Yukizaki C Hoshi Y Deguchi T Yoshida M 《Journal of agricultural and food chemistry》2008,56(11):4004-4011
Bitter gourd ( Momordica charantia L.) is a popular tropical vegetable in Asian countries. Previously it was shown that bitter gourd placenta extract suppressed lipopolysaccharide (LPS)-induced TNFalpha production in RAW 264.7 macrophage-like cells. Here it is shown that the butanol-soluble fraction of bitter gourd placenta extract strongly suppresses LPS-induced TNFalpha production in RAW 264.7 cells. Gene expression analysis using a fibrous DNA microarray showed that the bitter gourd butanol fraction suppressed expression of various LPS-induced inflammatory genes, such as those for TNF, IL1alpha, IL1beta, G1p2, and Ccl5. The butanol fraction significantly suppressed NFkappaB DNA binding activity and phosphorylation of p38, JNK, and ERK MAPKs. Components in the active fraction from bitter gourd were identified as 1-alpha-linolenoyl-lysophosphatidylcholine (LPC), 2-alpha-linolenoyl-LPC, 1-lynoleoyl-LPC, and 2-linoleoyl-LPC. Purified 1-alpha-linolenoyl-LPC and 1-linoleoyl-LPC suppressed the LPS-induced TNFalpha production of RAW 264.7 cells at a concentration of 10 microg/mL. 相似文献
123.
Growth of Salmonella typhimurium and Salmonella enteritidis in egg yolks from highly immunized hens.
K Takase T Nakayama T Kawai H Fujikawa 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(8):959-960
This experiment was conducted to ascertain whether the growth of Salmonella Enteritidis (SE) or Salmonella Typhimurium (ST) would be suppressed in the presence of antibodies contained in egg yolks. Specific pathogen-free chickens (102 days of age) were subcutaneously immunized with oil-adjuvanted bacterin of SE or ST, twice within a four-week interval. During 160 to 170 days of age, eggs were collected, the yolks were removed and mixed with an equal volume of physiological buffered saline, inoculated with ten colony forming units (CFU) of SE or ST, and incubated at 37 degrees C or 20 degrees C for 23 hr. The growth of organisms in each yolk solution was examined. The egg yolk derived from non-immunized hens was examined in the same manner as the controls. There was no difference in the growth titer between the antibody-positive yolk and the negative yolk. The result suggests that the antibodies in the yolk do not influence the growth of each organism, even if the hens are highly immunized. 相似文献
124.
Tomohiro Nakayama Hitomi Nakayama Mutsumi Miyamoto Robert L. Hamlin 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1999,13(5):485-490
We investigated the effects of graded dosages of magnesium given i.v. to anesthetized dogs on blood pressure, cardiac output, and electrophysiology. Magnesium was infused at 0.12 mEq/kg/minute until ventricular fibrillation occurred naturally or was provoked by programmed electrical stimulation or until arrest of the sinuatrial node in 8 dogs. Plasma total magnesium concentrations doubled in 1 minute of that infusion rate, and a hemodynamically safe plasma concentration of 12.2 mEq/L was achieved after 16 minutes of infusion. Heart rate, inotropy, lusitropy, and cardiac output increased up to a cumulative infusion dosage of magnesium of 1.0-2.0 mEq/kg, which produced plasma magnesium concentrations of 8.5-12.2 mEq/L (n = 5). Above the cumulative infusion dosage, inotropy decreased and lusitropy increased until death occurred between cumulative infusion dosages of 5.9 mEq/kg and 10.9 mEq/kg. Arterial pressure and vascular resistance decreased, and PQ interval and QRS complex increased, in a dose-dependent fashion. The relationship between ionized and total magnesium was y = 0.624x - 0.542 (r2 = .986), where y is ionized and x is total magnesium in mEq/L in 3 dogs. In conclusion, a cumulative infusion dosage of 0.1-0.2 mEq/kg of magnesium may be given without changing hemodynamic parameters, but with higher cumulative infusion doses heart rate accelerates. Hemodynamic parameters except those related to blood pressure continued to increase to a cumulative infusion dosage of 2.0 mEq/kg. At higher cumulative infusion dosages dogs became hypotensive and the PQ interval was prolonged. However, dangerous arrhythmias were not provoked until a total dosage of 3.9 mEq/kg. 相似文献
125.
Takuhito Nozoe Akira Uchino Shigenori Okawa Shuichi Yoshida Yukihide Kanda Yukinori Nakayama 《Soil Science and Plant Nutrition》2013,59(2):200-205
Laboratory experiments were conducted to investigate (1) the effects of the addition of rice (Oryza sativa. L.) bran to paddy soil on the germination of Monochoria vaginalis (Burm. f.) Kunth, and (2) the relationship between the electric conductivity (EC) of the soil solution and germination. Soil samples were collected at 4 sites in Japan. After flooded soils with rice bran had been incubated for 7?d at 30°C, the soil solution was collected using a porous cup and the EC of the soil solution was measured. The amounts of rice bran added to the soil were 0%, 0.3%, 0.6% and 0.9% (weight(w)/w). In the soil solution obtained, seeds of M. vaginalis were incubated for 3?d at 30°C, and the germination percentage was then analyzed. The addition of rice bran suppressed germination, and the degree of suppression increased with increasing content of rice bran. Although the same amount of rice bran was applied to each soil, the degree of growth suppression by rice bran as well as the EC of the soil solution differed among the soils. In each soil, there was a positive correlation between the amount of rice bran and EC, and the degree of growth suppression significantly increased with an increase in EC. When EC was higher than 150?mS?m?1, seeds of M. vaginalis hardly germinated. There was no significant correlation between the oxidation-reduction potential (Eh) of soil and seed germination, suggesting that EC is a more reliable and convenient indicator than Eh for evaluating the relationship between the addition of organic material and seed germination. In conclusion, the addition of rice bran to soil increases the EC of the soil solution, and EC is one of the factors that suppress the germination of M. vaginalis. The suppressive effect of rice bran on germination is different among soils. This fact is attributed to the difference in EC due to the addition of rice bran. Thus, it is expected that EC can be used as an indicator for determining how much rice bran to add. 相似文献
126.
Luanna do Carmo Neves Filipe Cipriano Joo Paulo Silva Lorenzini Kauana Santos de Lima Cipriano Lucas Pedro Gonalves Junior Cintia L. Nakayama Ronald Kennedy Luz Kleber Campos Miranda Filho 《Aquaculture Research》2019,50(10):2932-2937
Poecilia velifera is a popular fish in freshwater aquarism that naturally inhabits aquatic habitats with a wide range of salinities. Nonetheless, the effects of different salinities on the reproductive success and sex ratio of the species remain unknown. Male sex characters, sex ratio and reproductive success of P. velifera were evaluated by maintaining 160 juveniles (0.078 ± 0.011 g) in four different salinities (0; 12; 24; 36 g/L), with four replicates. The only modification observed in males was the formation of a copulatory organ from the anal fin, which was used to distinguish them from females. Timing of the formation of the male copulatory organ, and the weight and total length of males when it occurred, were recorded. Twenty‐eight fish from each treatment were euthanized after 150 day to examine the gonads and confirm sex. Eight females and four males from each treatment were then kept in their respective salinity treatments for 65 days to examine the effects of salinity on reproduction. Males were kept with females in the different treatments for 15 days, at which point they were removed. Salinity was negatively correlated with male weight and length. Salinity also affected the sex ratio, with the percentages of males and females being 32.5 and 67.5%, and 27.5 and 72.5% in the salinities of 24 and 36 g of salt/L, respectively. Fifty percent of the females kept in freshwater reproduced, while there was no reproduction in the other treatments. The maintenance of P. velifera in freshwater promoted greater reproductive success and precocity, as well as larger males. 相似文献
127.
128.
129.
Pathological studies on cerebral amyloid angiopathy, senile plaques and amyloid deposition in visceral organs in aged dogs. 总被引:1,自引:0,他引:1
K Uchida H Nakayama N Goto 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(6):1037-1042
The relationship between cerebral lesions such as amyloid angiopathy or senile plaques and amyloid deposition in the visceral organs were studied in 90 autopsy cases of dogs, 0 to 19-year-old. Cerebral amyloid angiopathy was detected in 28 aged dogs (mean age: 13.7-year-old) and was found mostly in or around the wall of cerebral meningeal arterioles and capillaries of the neocortex. That condition was often accompanied by cerebral hemorrhage in dogs more than 9 years of age. Senile plaques were detected in the neocortex of the brain of 12 dogs (mean age: 13.2-year-old) and classified into 3 subtypes, i.e., "diffuse plaque", "primitive plaque" and "classical plaque". Among those 3 subtypes of senile plaques, amyloid containing plaques were small in number. In the visceral organs of dogs with cerebral amyloid angiopathy, amyloid deposition was found in the vascular walls or connective tissues of small intestines at a high frequency and sometimes in the vascular walls of the heart, lung, liver and thyroid gland as well as in atrioventricular valves. Amyloid in both cerebral and visceral organs was congophilic and showed green birefringence under poralized light even after potassium permanganate oxidation. 相似文献
130.
Comparison of Some Pharmacokinetic Parameters of 5 Angiotensin-Converting Enzyme Inhibitors in Normal Beagles 总被引:1,自引:0,他引:1
Robert L. Hamlin Tomohiro Nakayama 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(2):93-95
This study was designed to determine the degree of inhibition of the angiotensin-converting enzyme (ACE) in 5 normal dogs given single doses of conventionally used ACE inhibitors (ACEis). In addition the time required for that inhibition to return to 50% of the difference between maximum and zero (control values) was measured as an estimate of duration of action. The 5 ACEis (with dosages given in parentheses) were bena/april (0.5 mg/kg), captopril (2.0 mg/kg), enalapril (0.5 mg/kg), lisinopril (0.5 mg/kg), and ramipril (0.25 mg/kg). Blood samples for ACE activities were obtained before dosing and at 1.5, 3.0, 6.0, 12.0, and 24.0 hours after dosing. All ACEis except captopril decreased ACE activities to approximately 25% of control by the 1.5-to 3.0-hour sample, and ACE activities returned to 50% of the difference by the 12-hour sample. The value of ACE activity returned to normal by 24 hours for benazapril, whereas values for ACE activity remained below normal for enalapril, lisinopril, and ramipril at 24 hours. For captopril, however, ACE levels decreased to approximately 80% of control by the 1.5-hour recording, and returned to levels not different from control by the 3-hour recording. Based upon this study performed on normal dogs given a single dose, no pharmacokinctic advantage or disadvantage is apparent for any ACEi except captopril, which, at the dosage used, decreased ACE levels to a much lesser degree and shorter time. 相似文献