Two cases of feline malignant craniopharyngioma   总被引：1，自引：0，他引：1  

Nagata T  Nakayama H  Uchida K  Uetsuka K  Yasoshima A  Yasunaga S  Masuda K  Tsujimoto H  Kuwajima E  Nishimura R  Sasaki N  Doi K 《Veterinary pathology》2005,42(5):663-665

Tumors at the cranial base in 2 cats (a 9 1/2-year-old, castrated male Chinchilla and a 7-year-old, castrated male American Shorthair) were diagnosed as malignant craniopharyngioma. The tumor lesion was histopathologically divided into four parts: 1) a small acinus part, in which relatively large cells with a pale cytoplasm composed small acini; 2) a duct part, in which small cuboidal cells composed ducts; 3) a cyst part, in which there were large cysts lined with flat cells; and 4) a pavement part, in which large multi-angular-shaped cells proliferated in a pavement pattern. The epithelial cells of some parts were positive for keratin by immunohistochemistry. Histopathologic findings of the present feline cases were identical to those of malignant craniopharyngioma in other animal species.  相似文献   

Secondary erythrocytosis associated with schwannoma in a dog     

Yamauchi A  Ohta T  Okada T  Mochizuki M  Nishimura R  Matsunaga S  Nakayama H  Doi K  Sasaki N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1605-1608

An 11-year-old, spayed female mixed-breed dog showed clinical signs of right forelimb lameness and pain by palpation around the neck. Radiography and computed tomography (CT) revealed an extradural mass at the 6th and 7th cervical vertebrae, which compressed the spinal cord. The mass was surgically removed and histopathologically diagnosed as schwannoma. The dog recovered her normal gait after hemilaminectomy and removal of the mass. Ten months after the surgery, the tumor recurred with absolute erythrocytosis and was surgically removed again. This removal temporarily resolved the erythrocytosis with a decrease in plasma erythropoietin (EPO) concentration. EPO protein was detected immunohistochemically in the tumor cells. Erythrocytosis in this dog may be caused by ectopic EPO produced in the schwannoma tissues.  相似文献   

Prevalence of autoantibody in cerebrospinal fluids from dogs with various CNS diseases     

Matsuki N  Fujiwara K  Tamahara S  Uchida K  Matsunaga S  Nakayama H  Doi K  Ogawa H  Ono K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(3):295-297

To examine the prevalence of autoantibody in canine cerebrospinal fluids (CSFs), CSFs were collected from 14 healthy controls and 88 clinical cases with various diseases in the central nervous system (CNS), and were analyzed by an indirect fluorescence antibody test on frozen sections of the cerebrum from normal Beagle dogs. An anti-astrocyte autoantibody was detected in 31 clinical cases with titers ranging from 1:1 to >/=1:100. All tested cases with necrotizing meningoencephalitis (NME: n=22) and granulomatous meningoencephalitis (GME: n=3) possessed the anti-astrocyte autoantibody, while the autoantibody was negative in most cases with other inflammatory CNS diseases. The autoantibody was also detected in 4 of 12 cases with brain tumors. Hence, examination of the autoantibody in the canine CFS would be significant for diagnosing NME and/or GME, as well as for understanding peritumoral events in cases with brain tumors.  相似文献   

Trickle irrigation water quality and preventive maintenance     

D.A. Bucks  F.S. Nakayama  R.G. Gilbert 《Agricultural Water Management》1979,2(2):149-162

The most serious problem in trickle irrigation is clogging of emitters or applicators. Recommendations and guidelines are presented for preventive maintenance which include water filtration, chemical treatment, pipeline flushing, and field inspection. A suitable type, size, and capacity of a filtration unit is required. Chemical treatment should be considered in terms of theory and field research on the reclamation and prevention of emitter clogging. Proper procedures for the flushing and field inspection of trickle irrigation systems are also essential. Because water quality is of primary importance in the design and operation of this maintenance program, a tentative water-classification system was developed to establish criteria by evaluating the clogging potential of a trickle irrigation water source.  相似文献   

Experimental infection of enterotoxemic Escherichia coli associated with porcine edema disease and its pathologic characteristics in the intestine     

Tsukahara T  Nakanishi N  Nakayama K  Matsubara N  Ushida K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(11):1167-1171

Edema disease (ED) has become frequent in Japan, but no effective method for experimental infection has been developed. We report here the use of a capsule that resistant against gastric digestion to induce the ED in piglets. Four 21-day-old piglets were used. Shiga toxin 2e-producing Escherichia coli (STEC) cell pellet was encapsulated and administered orally. Two pigs received 1.0x10(10) CFU for two days, and the others received 3.9x10(8) CFU for three days. The high-dose group caused the typical clinical ED signs (palpebral edema or neurologic impairment). Eosinophil infiltration, swollen lymphoid follicles, and edema were observed in the ileum. The kidney had the thrombus in the glomerulus.  相似文献   

Evaluation of the six-minute walk test in dogs     

Boddy KN  Roche BM  Schwartz DS  Nakayama T  Hamlin RL 《American journal of veterinary research》2004,65(3):311-313

OBJECTIVE: To determine the feasibility for use of a 6-minute walk test (6-MWT) in dogs with congestive heart failure (CHF) and document that the distance walked in 6 minutes decreases when a dog has CHF. ANIMALS: 16 young mature male hound-crossbred dogs weighing between 25 and 37 kg. PROCEDURE: An unobstructed path (22.73 m) was measured in a hallway. Each dog was walked on a leash for 6 minutes; each dog was allowed to set its own pace. At the end of 6 minutes (as measured by use of a stopwatch), the total distance walked was measured. Heart rate (HR) obtained by auscultation and mean systemic arterial pressure (MAP) obtained by oscillometry were recorded before and after the 6-MWT. Heart failure was induced by use of rapid ventricular pacing. Mean of the distance walked, HR, and MAP before and after the 6-MWT were compared between the control period and after dogs developed induced CHF. RESULTS: Dogs with CHF had a significant increase in resting HR, significant decrease in MAP, and a significant decrease in the distance walked in 6 minutes. The MAP increased slightly after exercise during the control period but decreased slightly after exercise during the CHF period. Fractional shortening decreased significantly when dogs had CHF. CONCLUSIONS AND CLINICAL RELEVANCE: Analysis of these results indicated that the distance walked in 6 minutes decreased significantly when a dog had CHF. The 6-MWT requires little time, space, or equipment and may replace the treadmill exercise test.  相似文献   

Intracranial meningioma with polygonal granular cell appearance in a Chihuahua     

Takeuchi Y  Ohnishi Y  Matsunaga S  Nakayama H  Uetsuka K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(5):529-532

A menigioma with polygonal granular cell proliferation in an 11-year and 8-month-old male Chihuahua is described. The tumor was observed under the dura matter of the right cerebrum. Microscopically, the tumor consisted of solid growth foci of small- or large- sized polygonal cells, with pale-stained nuclei, prominent nucleoli, and fine granular to foamy eosinophilic cytoplasm. Some of the proliferating cells contained variable amounts of cytoplasmic PAS-positive granules. Immunohistochemical analysis revealed that neoplastic cells were positive for vimentin and S-100 protein. Ultrastructurally, the neoplastic cells contained vesicular structures with a few small round-shaped bodies in the cytoplasm. We diagnosed the case as canine meningioma with granular cell appearance.  相似文献   

Characterization of equine cytochrome P450: role of CYP3A in the metabolism of diazepam          下载免费PDF全文

S. M. M. Nakayama  Y. Ikenaka  A. Hayami  H. Mizukawa  W. S. Darwish  K. P. Watanabe  Y. K. Kawai  M. Ishizuka 《Journal of veterinary pharmacology and therapeutics》2016,39(5):478-487

Research on drug metabolism and pharmacokinetics in large animal species including the horse is scarce because of the challenges in conducting in vivo studies. The metabolic reactions catalyzed by cytochrome P450s (CYPs) are central to drug pharmacokinetics. This study elucidated the characteristics of equine CYPs using diazepam (DZP) as a model compound as this drug is widely used as an anesthetic and sedative in horses, and is principally metabolized by CYPs. Diazepam metabolic activities were measured in vitro using horse and rat liver microsomes to clarify the species differences in enzyme kinetic parameters of each metabolite (temazepam [TMZ], nordiazepam [NDZ], p‐hydroxydiazepam [p‐OH‐DZP], and oxazepam [OXZ]). In both species microsomes, TMZ was the major metabolite, but the formation rate of p‐OH‐DZP was significantly less in the horse. Inhibition assays with a CYP‐specific inhibitors and antibody suggested that CYP3A was the main enzyme responsible for DZP metabolism in horse. Four recombinant equine CYP3A isoforms expressed in Cos‐7 cells showed that CYP3A96, CYP3A94, and CYP3A89 were important for TMZ formation, whereas CYP3A97 exhibited more limited activity. Phylogenetic analysis suggested diversification of CYP3As in each mammalian order. Further study is needed to elucidate functional characteristics of each equine CYP3A isoform for effective use of diazepam in horses.  相似文献   

Chemical constituents and physical properties of guayule wood and bark     

Poo Chow  Francis S. Nakayama  Brian Blahnik  John A. Youngquist  Terry A. Coffelt   《Industrial Crops and Products》2008,28(3):303-308

About 15 Parthenium species grow in the North American continent with Parthenium argentatum (guayule) as the only species containing harvestable amounts of the rubber latex. The predicted commercialization of the guayule plant for its hypoallergenic latex will result in a significant amount of waste fiber or bagasse biomass that can also be put to use for making wood, paper, and other chemical products, as well as in energy production. Thus, the guayule wood and bark fibers can be considered a new source of plant biomass that may be used as a direct substitute for forest-based wood fiber. However, little information is available on the chemical composition of the wood and bark tissues of guayule (P. argentatum). The objectives of this study were to determine the chemical and fiber composition of guayule and to compare it with other wood fiber sources.Three germplasm lines of mature guayule (Cal-6, AZ-101, and G7-15) and another species of Parthenium (P. tomentosum, PT), juvenile soft maple (Acer rubrum), a deciduous tree, and milkweed (Asclepias syriaca L.) that has long fibers were the plant sources. Separate wood and bark tissues were analyzed for hot water, 1% sodium hydroxide, and alcohol–toluene extracts. In addition, the lignin, holocellulose, alpha-cellulose, and pentosan contents were determined.All the chemical components in the wood fibers for the Partheniums were equal to or greater than the juvenile maple tree. Milkweed had higher alpha-cellulose and lower alcohol–toluene extract contents than both the guayule and soft maple. The guayule bark fibers had more chemical extracts than the wood fibers. The specific gravity of guayule wood was greater than the deciduous wood species. However, the fiber lengths of soft maple wood, guayule wood, and milkweed are similar. Based on the chemical composition, P. argentatum and P. tomentosum could serve as raw materials for the paper and chemical industries as well as for energy production.  相似文献   

Simple and sensitive BIO-PCR detection of potato blackleg pathogens from stem,tuber, and soil samples     

Aono  Yoshiyuki  Nakayama  Takato  Ozawa  Takahiro  Ushio  Yutaka  Yasuoka  Shinji  Fujimoto  Taketo  Ohki  Takehiro  Oka  Norikuni  Maoka  Tetsuo 《Journal of General Plant Pathology》2021,87(4):209-218

Journal of General Plant Pathology - A BIO-PCR was developed for the detection of potato blackleg pathogens, including Pectobacterium wasabiae (Pw), P. atrosepticum (Pa), P. carotovorum subsp....  相似文献