Effect of temperature and/or pressure on tomato pectinesterase activity     

Van Den Broeck I  Ludikhuyze LR  Van Loey AM  Hendrickx ME 《Journal of agricultural and food chemistry》2000,48(2):551-558

The activity of tomato pectinesterase (PE) was studied as a function of pressure (0.1-900 MPa) and temperature (20-75 degrees C). Tomato PE was rather heat labile at atmospheric pressure (inactivation in the temperature domain 57-65 degrees C), but it was very pressure resistant. Even at 900 MPa and 60 degrees C the inactivation was slower as compared to the same treatment at atmospheric pressure. At atmospheric pressure, optimal catalytic activity of PE was found at neutral pH and a temperature of 55 degrees C. Increasing pressure up to 300 MPa increased the enzyme activity as compared to atmospheric pressure. A maximal enzyme activity was found at 100-200 MPa combined with a temperature of 60-65 degrees C. The presence of Ca(2+) ions (60 mM) decreased the enzyme activity at atmospheric pressure in the temperature range 45-60 degrees C but increased enzyme activity at elevated pressure (up to 300 MPa). Maximal enzyme activity in the presence of Ca(2+) ions was noted at 200-300 MPa in combination with a temperature of 65-70 degrees C.  相似文献   

Implications of beta-mercaptoethanol in relation to folate stability and to determination of folate degradation kinetics during processing: a case study on [6S]-5-methyltetrahydrofolic acid     

Indrawati I  Verlinde P  Ottoy F  Van Loey A  Hendrickx M 《Journal of agricultural and food chemistry》2004,52(26):8247-8254

The effect of beta-mercaptoethanol (0-2%) addition to thermally and/or pressure-treated samples on [6S]-5-methyltetrahydrofolate was studied. Degradation of [6S]-5-methyltetrahydrofolate during both thermal and pressure treatments was mainly caused by oxidation, and the oxidized folates could be partly/completely reduced by beta-mercaptoethanol. The addition of beta-mercaptoethanol (2%) to the thermally and pressure-treated samples overestimated the "actual" stability of [6S]-5-methyltetrahydrofolate and misled the obtained kinetic information.  相似文献   

Model studies on the stability of folic acid and 5-methyltetrahydrofolic acid degradation during thermal treatment in combination with high hydrostatic pressure     

Nguyen MT  Indrawati  Hendrickx M 《Journal of agricultural and food chemistry》2003,51(11):3352-3357

Stability of folic acid and 5-methyltetrahydrofolic acid in phosphate buffer (0.2 M; pH 7) toward thermal (above 65 degrees C) and combined high pressure (up to 800 MPa)/thermal (20 up to 65 degrees C) treatments was studied on a kinetic basis. Residual folate concentration after thermal and high pressure/thermal treatments was measured using reverse phase liquid chromatography. The degradation of both folates followed first-order reaction kinetics. At ambient pressure, the estimated Arrhenius activation energy (E(a)) values of folic acid and 5-methyltetrahydrofolic acid thermal degradation were 51.66 and 79.98 kJ mol(-1), respectively. It was noticed that the stability of folic acid toward thermal and combined high pressure thermal treatments was much higher than 5-methyltetrahydrofolic acid. High-pressure treatments at room temperature or higher (up to 60 degrees C) had no or little effect on folic acid. In the whole P/T area studied, the rate constant of 5-methyltetrahydrofolic acid degradation was enhanced by increasing pressure, and a remarkable synergistic effect of pressure and temperature on 5-methyltetrahydrofolic acid degradation occurred at temperatures above 40 degrees C. A model to describe the combined pressure and temperature effect on the 5-methyltetrahydrofolic acid degradation rate constant is presented.  相似文献   

Kinetics of Zn and Cd accumulation in the isopod Porcellio scaber exposed to contaminated soil and/or food     

Martina G. Vijver  Jos P.M. Vink  Nico M. van Straalen  Cornelis A.M. van Gestel 《Soil biology & biochemistry》2006,38(7):1554-1563

To derive accumulation kinetics from different exposure matrices and account for the contribution of different exposure routes, the isopod Porcellio scaber was exposed to Cd and Zn, either in soil or in food, and a combination of both. Accurate uptake and elimination kinetics of Zn and Cd were determined using radioisotopes, allowing non-destructive time measurements. To describe the data, a simple kinetic model was used accounting for the internal distribution of the metals. A strong influence of the elimination kinetics, together with the metal fraction that is compartmentalized in a storage fraction, was found explaining the Cd steady-state level reached in the isopods. Zn turnover kinetics were relatively fast within the first days of exposure and elimination, followed by slow Zn kinetics from the storage compartment. The storage fraction appeared to be inert for elimination of both Zn and Cd within the experimental duration of 32 days and the fraction of metal taken up that was stored in this fraction was influenced by the uptake source. The quantity of metals taken up from soil or food depended on the concentration. For both Zn and Cd, the uptake rate constant from soil equalled the uptake rate constant from food, moreover, the uptake from both routes was shown to be additive. Therefore, it is concluded that the relative contribution of both routes (via soil or food) is explained by the partitioning of metals between soil and food.  相似文献   

Microbial links and element flows in nested detrital food-webs     

Andrei D. Pokarzhevskii  Nico M. van Straalen  Dmitrii P. Zaboev  Andrei S. Zaitsev 《Pedobiologia》2003,47(3):213-224

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Prediction of wetting front stability in dry field soils using soil and precipitation data   总被引：1，自引：0，他引：1  

Jan M. H. Hendrickx  Tzung-mow Yao 《Geoderma》1996,70(2-4):265-280

A need exists for information regarding the stability of wetting fronts in field soils because they increase the vulnerability for groundwater contamination. In this study, we develop a simple approach for the evaluation of wetting front stability in dry soils. We show that the stability of wetting fronts in the top layer of a soil depends both on the type of soil and the intensity of the precipitation. Our approach distinguishes stability criteria for wetting events that are different for a high, intermediate, and low infiltration rate. At high infiltration rates, wetting fronts are stable if the infiltration rate exceeds or equals the saturated hydraulic conductivity of the soil. The stability criterion for low infiltration rates (less than approximately 0.2 cm/h for sand soils) is based on two characteristics times[ a gravitational time and an infiltration time. The gravitational time, t_grav, indicates when gravity and capillarity each contribute equally to the process of infiltration. The infiltration time, t_infil, is the duration of the infiltration event. Experimental and literature data show that in well-sorted laboratory sands, wetting fronts are stable when t_infil < 0.002 t_grav. This expression can also be expressed as Wi < 0.002 S² with W the total amount of precipitation, i its intensity, and S the sorptivity at a slightly positive soil-water pressure. For intermediate infiltration rates, wetting fronts remain stable as long as W is smaller than the amount of water needed to wet a distribution layer near the surface. The application of the stability criteria is demonstrated with a case study from the Sevilleta dunes near Socorro, NM.  相似文献   

Effects of cryostabilizers, low temperature, and freezing on the kinetics of the pectin methylesterase-catalyzed de-esterification of pectin     

Terefe NS  Delele MA  Van Loey A  Hendrickx M 《Journal of agricultural and food chemistry》2005,53(6):2282-2288

The kinetics of the pectin methylesterase (PME)-catalyzed de-esterification of pectin was studied at 25 degrees C in the presence of sucrose, fructose, maltodextrin (DE = 16.5-19.5), and carboxymethylcellulose at different concentrations and in the presence of maltodextrin and sucrose at different concentrations in a temperature range between +25 and -4 degrees C in subcooled and frozen states. The objective was to determine whether the reaction is diffusion-controlled, to gain insight about the factors determining the diffusion of the reactants, and to determine the effect of the carbohydrates, low temperature, and freezing on the structural conformation of the enzyme. The results indicate that the PME-catalyzed de-esterification of pectin is diffusion-controlled. Nevertheless, the diffusion is not controlled by the macroviscosity of the reaction medium, but rather by the microviscosity experienced by the diffusants. Low temperature in the temperature range studied does not affect the structural conformation of the enzyme, while freezing seems to have some effect.  相似文献   

Metal concentrations in soil and invertebrates in the vicinity of a metallurgical factory near Tula (Russia)     

Nico M. van Straalen  Ruslan O. Butovsky  Andrei D. Pokarzhevskii  Andrei S. Zaitsev  S. Cornelis Verhoef 《Pedobiologia》2001,45(5):451

To investigate the effects of emissions from a large metal works near Tula in the Russian Federation, we measured concentrations of iron, manganese, zinc, copper, nickel, lead and cadmium in soil, litter and invertebrates at four sampling sites at different distances from the factory. The sites were located in woodlands in the bed of the Voronka river, near the town of Kosaya Gora in the district of Tula. Additional soil properties (organic matter content, clay content, water holding capacity, Ca, Mg, N, P, and pH) were measured that could explain differences in the bioavailability of the metal burdens. It appeared that the factory is a source of Fe, Mn, Zn, Cu, Ni and Pb. One of the sampling sites had a high nitrogen content in the litter due to emissions from a fertilizer plant in the area. Most of the metal contamination was limited to the immediate surroundings and did not extend beyond a distance of 5 km. Only the site close to the factory can be considered as polluted, however, background concentrations of metals in the Tula area seem to be significantly lower than in present Western European soils and a reference system still has to be developed. Exchangeable metal concentrations (0.01 M CaCl₂ extracts from soil) were very low and were not correlated with the total concentrations, indicating low bioavailability of the pollution. At the most polluted site, concentrations of all metals were positively correlated with each other; correlations decreased with increasing distance. Metal concentrations in soil were often negatively correlated with organic matter content, especially so for nickel. Metal concentrations in invertebrates showed considerable variation between individual species, however, some general patterns were obvious. Concentrations were high in earthworms, oribatid mites and carabid beetles, and low in springtails, centipedes and spiders. There was no relationship between the trophic position of a species and its metal accumulating ability. Iron concentrations in invertebrates at the polluted site were a factor of 2 to 4 higher than at the most remote (reference) site; for zinc and copper the internal concentrations were also elevated, but to a lesser extent than the soil concentrations. The data illustrate the extremely complicated relationship between metal residues in invertebrates and metal concentrations in soil. For most of the saprophageous and predatory arthropods studied total concentrations nor exchangeable concentrations in soil are good predictors; species-specific feeding mechanisms and metal physiologies seem to be the main determinants.  相似文献   

Pressure and temperature stability of 5-methyltetrahydrofolic acid: a kinetic study     

Indrawati I  Van Loey A  Hendrickx M 《Journal of agricultural and food chemistry》2005,53(8):3081-3087

Detailed kinetic studies of [6S] and [6RS] 5-methyltetrahydrofolic acid (5-CH3-H4folate) degradation during thermal (from 60 to 90 degrees C) and high pressure/thermal (from 30 to 45 degrees C; from 200 to 700 MPa) treatments were carried out. The results confirmed that the temperature and pressure induced degradation kinetics of [6S] 5-CH3-H4folate were identical (within 95% confidence interval) with those of [6RS] 5-CH3-H4folate. Under equal processing conditions, the estimated degradation rate constants (k), activation energy (E(a)), and activation volume (V(a)) values of [6S] and [6RS] 5-CH3-H4folate were the same (95% confidence interval). The modified thermodynamic model proposed by Nguyen and co-workers (J. Agric. Food Chem. 2003, 51, 3352-3357) to describe the pressure and temperature dependence of the rate constant for folate degradation was reevaluated.  相似文献   

Species diversity and metal accumulation in oribatid mites (Acari, Oribatida) of forests affected by a metallurgical plant     

Andrei S. Zaitsev  Nico M. van Straalen 《Pedobiologia》2001,45(5)

A study was made of oribatid mite communities and their responses to metal contamination derived from the Kosogorsky metallurgical plant located in the Tula District, Russia. Mite communities were sampled in three different seasons in four sites at different distances from the smelter. Concentrations of zinc, copper, cadmium, lead and iron were measured in ten mite species. The data show that the mite community as a whole is quite tolerant to the metal contamination caused by the Kosogorsky plant. At the polluted sites some species typical for nutrient-rich soils appeared, but community structure and species diversity were not seriously affected by metal contamination. In general, oribatid mites accumulated metals to very high internal concentrations. Average Cu, Cd, Pb and Fe concentrations did not differ significantly between species, but Zn did, and its level was associated with the mode of feeding of the species. Microphytophagous species, feeding exclusively on fungi, accumulated zinc in higher concentrations than other mites. This study illustrates the potential for bioindication by the little studied but species-rich group of soil oribatids.  相似文献