Anthropogenic Heavy Metal Pollution in the Surficial Sediments of the Keratsini Harbor, Saronikos Gulf, Greece   总被引：1，自引：0，他引：1  

Stavroula Galanopoulou  Andreas Vgenopoulos  Nikolaos Conispoliatis 《Water, air, and soil pollution》2009,202(1-4):121-130

The contents of ten elements [Cd, Pb, W, Zn, Mn, As, Se, Cr, Cu, and organic carbon (C_org)] have been determined in the surficial sediments of Keratsini harbor, Saronikos Gulf, Greece. The contamination of the sediments was assessed on the basis of geoaccumulation index and to corresponding sediment quality guidelines (SQGs) effects range low/effects range median. The results revealed highly elevated Cd, Pb, W, Zn, As, Se, Cr, Cu, and C_org values (Cd, 190–1,763 mg kg^?1; Pb, 521–1,263 mg kg^?1; W, 38–100 mg kg^?1; Zn, 409–6,725 mg kg^?1; Mn, 95–1,101 mg kg^?1; As, not detectable–1,813 mg kg^?1; Se, not detectable–58 mg kg^?1; Cr, 264–860 mg kg^?1; Cu, 195–518 mg kg^?1; and C_org, 0.69–4.41%). The enrichment of metals in the sediments results from the contribution of the central Athens sewage outfall through which the waste of the Attica basin ends up in Keratsini harbor as well as from industrial and ship contaminants.  相似文献   

Teat disorders predispose ewes to clinical mastitis after challenge with Mannheimia haemolytica     

Mavrogianni VS  Cripps PJ  Papaioannou N  Taitzoglou I  Fthenakis GC 《Veterinary research》2006,37(1):89-105

In order to study the effects of sheep teat disorders on the protection of the mammary gland, we used a Mannheimia haemolytica isolate, which did not cause clinical mastitis when deposited into intact teats. In the first experiment, this was deposited into the duct of teats with orf (Group A, n=5) or papilloma (Group B, n=3). In the second, teats were chapped and then, the organism was deposited into the duct (Group C, n=7) or on the skin (Group D, n=4). Ewes with healthy teats were controls (Group E, deposition into duct, n=5; Group F, deposition on skin, n=2). The ewes in Groups A, B or C developed clinical mastitis 5 h later, whilst the ewes in Group D developed it 2 d later; no control ewe developed clinical mastitis. In ewes with teat lesions, the organism was isolated from secretion samples and the California Mastitis Test became positive 5 h after challenge; neutrophils and lymphocytes were seen in Giemsa-stained secretion films from Group A or B ewes, whilst macrophages, neutrophils and lymphocytes in films from Group C or D ewes; neutrophils were predominating in films from Group E or F ewes. Inside the teats of Group A, B, C or D ewes, folds, hyperaemia and mucosal thickness were seen; histologically, subepithelial leucocytic infiltration was seen. In Group A or B ewes, no evidence of lymphoid tissue at the teat duct-cistern border was found. In Group C or D ewes, intense erosion and ulceration of the teat skin and conspicuous lymphoid tissue at the teat duct-cistern border, were evident; lesions characteristic of haemorrhagic mastitis were in the mammary parenchyma. In control ewes, subepithelial leucocytic infiltration in the teat duct and lymphoid tissue as above, were evident. We postulate that teat lesions can be predisposing factor to mastitis, by adversely affecting defences and speeding the process of infection and making it more severe.  相似文献   

Effects of rearing density and tank colour on juvenile sharpsnout seabream (Diplodus puntazzo) growth performance     

Nafsika Karakatsouli  Nikolaos Kassianos  Sofronios E. Papoutsoglou 《Aquaculture International》2015,23(4):943-953

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Rapid method for simultaneous quantitative determination of four major essential oil components from oregano (Oreganumsp.) and thyme (Thymus sp.) using FT-Raman spectroscopy     

Siatis NG  Kimbaris AC  Pappas CS  Tarantilis PA  Daferera DJ  Polissiou MG 《Journal of agricultural and food chemistry》2005,53(2):202-206

A simple and rapid method for the quantitative determination of four major components found in oregano and thyme essential oils is presented. The method correlates the Raman peak intensity in the spectral region from 1800 to 600 cm(-1) and the concentration percentage of each particular constituent in the sample. To achieve accurate quantification results and avoid the risk of overlapping peaks of unknown Raman-active substances in natural essential oils, the peaks must be analyzed. For this purpose, PEAKSOLVE software (Ver. 1.0.5) was used. Unknown samples were measured with the FT-Raman method, and the results were compared to those of the gas chromatographic (GC) analysis. The comparison was made at a confidence level of 99%, and the two methods scored equally in terms of repeatability and accuracy even at the edge of the method specifications. The new method can provide accurate results in very short times once the setup is complete and could be utilized in areas where vast amounts of samples must be analyzed.  相似文献   

Estimation of scavenging activity of phenolic compounds using the ABTS(*+) assay     

Nenadis N  Wang LF  Tsimidou M  Zhang HY 《Journal of agricultural and food chemistry》2004,52(15):4669-4674

Observations on the applicability of the ABTS(*+) assay to define structure-activity relationships (SARs) among phenols (AH) were based on experimental data and theoretical calculations. All AH examined (hydroxycinnamic derivatives, simple polyphenols, polyhydroxybenzoates, and flavonoids) were found to be active toward ABTS(*+). Moreover, known weak radical scavengers (i.e., coumaric and isoferulic acids) were found to be efficient or comparatively active to caffeic or rosmarinic acids in contradiction to the AH classification based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) data or the bond dissociation enthalpy values. This behavior was observed both in ethanol and in buffered (pH 7.4) environment. Resorcinol and phloroglucin were found to be more active than catechol and hydroquinone, whereas, among polyhydroxybenzoates, 2,4-dihydroxybenzoic acid was the least active, in line with the DPPH and theoretical data. Therefore, it can be argued that the ABTS(*+) assay may give an indication for the presence of antioxidants in a certain system but SARs cannot be readily inferred.  相似文献   

The Effects of Seasonality in Estimating the C‐Factor of Soil Erosion Studies     

Thomas K. Alexandridis  Anastasia M. Sotiropoulou  George Bilas  Nikolaos Karapetsas  Nikolaos G. Silleos 《Land Degradation \u0026amp; Development》2015,26(6):596-603

Monitoring soil erosion risk is an important part of soil conservation practices. It is usually estimated with the Universal Soil Loss Equation, and the C‐factor (vegetation cover) is derived from optical satellite images. However, because of lack of data and resources, or in rapid assessments, C‐factor is estimated using one or a few satellite observations, despite being temporally variable according to plants' phenology. The aim of this work was to study the effect of seasonality in estimating C‐factor. This was achieved by demonstrating first that there is a difference when estimating soil erosion with Universal Soil Loss Equation at variable time steps in a year, namely once, seasonally and monthly. Using Moderate Resolution Imaging Spectroradiometer normalized difference vegetation index images and statistical analysis at subcatchment scale, it was shown that there is a significant difference when estimating mean annual soil loss with the aforementioned temporal options. The highest differences were observed between monthly and annual time steps. The second objective was to identify which is the optimum time to estimate C‐factor in a year. The results show that November, October and March are the optimum months for single image estimation of annual soil erosion. Statistical analysis with a random point dataset suggested that the spatial variability of the results was influenced by the land cover type, especially in areas with variable leaf cover where a single date estimation of C‐factor was not representative of the whole year, such as annual crops and deciduous trees. Copyright © 2013 John Wiley & Sons, Ltd.  相似文献   

Influence of wine pH on changes in color and polyphenol composition induced by micro-oxygenation     

Kontoudakis N  González E  Gil M  Esteruelas M  Fort F  Canals JM  Zamora F 《Journal of agricultural and food chemistry》2011,59(5):1974-1984

The presence of oxygen in red wine leads to the transformation of ethanol into ethanal, which after capturing a proton will react with flavanols to start the process of forming ethyl bridges between flavanols and between flavanols and anthocyanins. Wine pH also conditions the equilibrium between the different anthocyanin structures and may thus affect anthocyanin reactivity. Consequently, the aim of this paper was to study how the pH can affect the changes induced by micro-oxygenation in two wines with different phenolic composition. The differences between micro-oxygenated wines and their controls were, in general, greater when the pH was more acidic. Specifically, the differences between micro-oxygenated wines and their corresponding controls in terms of color intensity, anthocyanin concentration, PVPP index, ethyl-linked pigments, B-type vitisins, polymeric pigments, and ethylidene-bridged flavanols were greater at lower pH. In contrast, the effects of micro-oxygenation when the pH was less acidic were much less evident and sometimes practically nonexistent. These results demonstrate for the first time that the pH of the wine has a great influence on oxygen-induced changes of color and phenolic compounds.  相似文献   

To what extent are threatened European plant species conserved in seed banks?     

Sandrine Godefroid  Stéphane Rivière  Steve Waldren  Nikolaos Boretos  Ruth Eastwood  Thierry Vanderborght 《Biological conservation》2011,(5):1494-1498

The number of ex situ conservation facilities has grown dramatically in recent years, and they have become increasingly integrated under national and regional conservation initiatives. However, little information is available about the extent to which plant species are appropriately represented in ex situ collections. This paper assesses whether seed/spore collections stored in European seed banks cover or not, and to what extent, the species currently threatened with extinction in the wild. Although a substantial amount of the European flora (ca. 70%) is currently stored in seed banks, we highlight the relatively poor representation of threatened species: only 27% of the taxa listed on the European threatened plant list and 44% of the taxa listed in Annex II of the EU Habitat Directive are stored in European seed banks. Some taxonomical groups most at risk, e.g. Pteridophytes and Orchidaceae, are also under-represented in European seed banks. By examining the number of accessions per species and the number of seeds per accession, this study also gave some insight on how well species are conserved, considering that these two variables are surrogates for genetic diversity. We have highlighted that at least two thirds of the threatened species stored in European seed banks likely suffer from too low genetic diversity in the collections. These analyses were essential to identify those collections and additionally the standards needed to maximize the usefulness of future collections.  相似文献   

Treatment with DAV for advanced-stage hemangiosarcoma in dogs     

Dervisis NG  Dominguez PA  Newman RG  Cadile CD  Kitchell BE 《Journal of the American Animal Hospital Association》2011,47(3):170-178

Hemangiosarcoma (HSA) is an aggressive disease that is fairly common in the dog. The authors evaluated a doxorubicin, dacarbazine, and vincristine (DAV) combination protocol in dogs with nonresectable stage II and stage III HSA. Twenty-four dogs were enrolled in this prospective, phase 2 study. Doxorubicin and dacarbazine were administered on day 1 while vincristine was administered on days 8 and 15. The protocol was repeated every 21 days for a maximum of six cycles or until disease progression. Toxicity and efficacy were assessed by clinical and laboratory evaluation and by questionnaires completed by the owners. Of the 24 included dogs, 19 were evaluable for response. The response rate (including five complete responses and four partial responses) was 47.4%. Median time to tumor progression was 101 days and median overall survival was 125 days. Significant toxicities were noted, including 41 high-grade hematologic and 12 high-grade gastrointestinal toxic events. Five dogs discontinued treatment due to chemotherapy-related toxicities, but no treatment-related deaths occurred. Multivariate analysis identified patient age (relative risk [RR], 2.3, P=0.049) to be negatively associated with time to progression whereas dacarbazine dose reductions (RR, 0.06, P=0.031) were positively associated with time to progression. Dacarbazine dose reduction was the sole factor positively associated with overall survival (RR, 0.28, P=0.015). In conclusion, the DAV combination appears to offer clinical responses and may prolong survival in dogs with advanced-stage HSA.  相似文献   

Association of bulk tank milk urea nitrogen concentration with elevated individual cow values and investigation of sampling frequency for accurate assessment     

Siachos  Nektarios  Panousis  Nikolaos  Valergakis  Georgios E. 《Tropical animal health and production》2019,51(8):2431-2436

Tropical Animal Health and Production - Individual milk urea nitrogen (MUN) levels ≥?19.63 mg/dL have been recently reported to significantly affect fertility. The objectives...  相似文献