翠竹及菲白竹LEA3同源基因的克隆及序列分析     

黄小云  王奇  牛伟超  林福永  鄢波  黄美娟  黄海泉 《分子植物育种》2020,(4):1109-1115

竹类植物在中国有着非常悠久的栽培历史,并被广泛应用于园林、造林、庭院以及食品等行业。本研究以翠竹和菲白竹为材料提取其叶片总DNA,采用PCR方法获得翠竹SpLEA3基因与菲白竹SfLEA3-1、SfLEA3-2基因;其中SpLEA3全长804 bp,编码195个氨基酸,GC含量为68.4%;SfLEA3-1全长803 bp,编码195个氨基酸,GC含量为68.2%;SfLEA3-2全长557 bp,编码144个氨基酸,GC含量为67.8%。通过ProtParamy等生物软件分析,SpLEA3、SfLEA3-1和SfLEA3-2与贵州悬竹LEA3基因同源性高达61%以上,且3个基因编码蛋白均属于亲水性蛋白。源自2个竹种LEA3基因均包含一个完整的开放阅读框,其编码的氨基酸含有4~6个由11个氨基酸组成的保守基元序列。本研究不仅为深入了解竹类植物抗旱的分子机理研究提供了基础数据,也为竹类植物的抗旱育种后续研究提供了科学依据。  相似文献   

爆裂玉米3个膨爆特性的非条件和条件QTL分析   总被引：2，自引：0，他引：2  

李玉玲  董永彬  牛素贞 《分子植物育种》2006,4(3):372-380

膨化倍数(PF)、膨化体积(PV)和爆花率(PR)是爆裂玉米的主要品质指标。本研究以普通玉米自交系丹232和爆裂玉米自交系N04杂交构建的259个F2:3家系为定位群体,采用完全随机区组设计在郑州夏播条件下测定了3个膨爆特性指标。利用覆盖玉米10条染色体的183对多态性分子标记构建连锁图,采用复合区间作图(CIM)方法对各性状进行非条件QTL定位分析,采用条件遗传统计软件对PF进行条件QTL分析。3个膨爆特性指标非条件QTL定位共检测出31个QTL,单个QTL的贡献率为3.09%￣12.46%,累计贡献率为51.90%、77.37%和61.45%。在8个标记区间(占42.11%)同时检测到控制2￣3个性状的QTL。加性和部分显性在膨爆特性的遗传中起主要作用,同时存在显性和超显性基因效应。条件QTL定位结果表明,PV和PR以不同方式显著影响PF的QTL表达,PV比PR更重要。  相似文献   

加强大学生利他行为培养的思考   总被引：4，自引：0，他引：4  

牛巨龙  田爱民 《高等农业教育》2006,(5):87-89

随着现代化进程的深入,社会结构的转型,大学生利他行为状况却与理想预期相距甚远。本文在分析大学生利他行为弱化原因的基础上,从制度建设层面阐述了培养大学生利他行为的方法和途径。  相似文献   

盘形振动上料器的一种系统模型和模拟   总被引：1，自引：0，他引：1  

许光驰  吕伟新  钮艳华 《东北林业大学学报》2007,35(6):96-98,101

推导出一种盘形振动上料器的数学模型,在此基础上分析了固有频率、振型等动态性能,进行了系统参数计算。并利用动态分析软件ADAMS进行了建模仿真,可直观地研究零件的一致化排列过程和输送的速度。实践证明,利用这种模型进行盘形振动上料器的研制工作十分有效。  相似文献   

四种菊酯类杀虫剂对枸杞蚜虫线粒体膜流动性的影响     

李海平  张丛  牛婧玮  常静  刁青云 《植物保护学报》2019,46(1):224-229

为明确菊酯类杀虫剂对昆虫线粒体膜流动性的影响,以1,6-二苯基-1,3,5-己三烯（1,6-diphenyl-1,3,5-hexatriene,DPH）为荧光探剂,采用荧光偏振法研究了4种菊酯类杀虫剂对枸杞蚜虫Aphis sp.线粒体膜流动性的影响。结果表明,4种菊酯类杀虫剂对枸杞蚜虫线粒体膜流动性均有显著影响,其荧光偏振度显著增大,线粒体膜流动性显著减小。当药剂浓度为100 μmol/L时,对照组枸杞蚜虫线粒体膜的荧光偏振度为0.239,高效氯氰菊酯的影响最大,线粒体膜的荧光偏振度为0.315,联苯菊酯的影响最小,线粒体膜的荧光偏振度为0.287。4种菊酯类杀虫剂对线粒体膜流动性的影响都存在一定的浓度效应,当药剂浓度在0~100 μmol/L范围内,浓度越大影响越大。4种菊酯类杀虫剂在不同温度下对枸杞蚜虫线粒体膜流动性的影响不同,高效氯氰菊酯、联苯菊酯和甲氰菊酯对线粒体膜流动性的影响基本不受温度影响,而氯菊酯在高温条件下产生的影响大,在低温条件下产生的影响小,表现为明显的正温度系数。表明DPH可以作为荧光探剂研究枸杞蚜虫线粒体膜的流动性。  相似文献   

Effect of N-acetyl cysteine and glycine supplementation on growth performance,glutathione synthesis,and antioxidative ability of grass carp, <Emphasis Type="Italic">Ctenopharyngodon idella</Emphasis>     

Shiwei Xie  Lixia Tian  Jin Niu  Guiying Liang  Yongjian Liu 《Fish physiology and biochemistry》2017,43(4):1011-1020

An 8-week feeding trial was conducted to evaluate the effect of N-acetyl cysteine (NAC) and glycine supplementation on growth performance, glutathione (GSH) synthesis, and antioxidative ability of grass carp, Ctenopharyngodon idella. Four practical diets were formulated: control, control + 0.2% NAC, control + 0.5% glycine, and control + 0.2% NAC + 0.5% glycine. Each diet was randomly assigned to quadruplicate groups of 30 fish (approximately 8.8 g). Weight gain and specific growth rate were significantly increased with the supplementation of NAC. Supplementation of NAC plus glycine significantly increased the feed efficiency. Glutathione peroxidase (GPx) and γ-glutamine cysteine synthase (γ-GCS) in plasma were significantly increased with the supplementation of NAC plus glycine. GSH in plasma increased and malondialdehyde (MDA) decreased in fish fed diets supplemented with NAC. Respiratory burst, superoxide dismutase (SOD), and catalase (CAT) activity were not affected by NAC or glycine. These results clearly indicated that NAC improved the growth performance and restored GSH of grass carp, supplemented NAC together with glycine enhanced GSH synthesis, and improved the antioxidative ability of grass carp.  相似文献   

Effect of l‐ascorbyl‐2‐polyphosphate supplementation on growth performance,body composition,antioxidative capacity and salinity stress tolerance of juvenile Pacific white shrimp,Litopenaeus vannamei          下载免费PDF全文

Shi‐Jun Chen  Yu‐Jie Gao  Shi‐Wei Xie  Jin Niu  Fan Yang  Wei‐Ping Fang  Li‐Xia Tian  Yong‐Jian Liu 《Aquaculture Research》2017,48(9):4608-4622

An 8‐week feeding trial was conducted to evaluate the effects of ascorbic acid (AsA), in the form of l ‐ascorbyl‐2‐polyphosphate (LAPP) on growth performance, body composition, antioxidative capacity and salinity stress tolerance of juvenile Pacific white shrimp, Litopenaeus vannamei. Five practical diets (46% crude protein and 7.6% lipid) supplemented with graded levels of AsA (14.64, 48.55, 84.98, 308.36 and 639.27 mg kg^?1 diet) were fed to five replicate groups of L. vannamei (mean initial wet weight 0.57 g). No significant differences were found on growth performance among all treatments. However, whole body lipid content significantly decreased with dietary AsA levels increasing. Activities of total antioxidant capacity, glutathione reductase and glutathione peroxidase were significantly affected by dietary AsA levels. Shrimp fed LAPP‐free diet had higher malondialdehyde content than those fed the diets supplemented with LAPP. Dietary AsA levels higher than 308.36 mg kg^?1 diet increased the survival of shrimps after 1, 2 and 3 h of acute salinity change. Broken‐line regression analysis on survival after 3 h of salinity stress and second‐degree polynomial regression analysis on glutathione reductase data indicated that the optimal dietary AsA requirement of L. vannamei was estimated to be 306.39, 319.75 mg kg^?1 diet respectively.  相似文献   

Occurrence of multiple antibiotic resistance and genotypic characterization in Edwardsiella tarda isolated from cage‐cultured hybrid red tilapia (Oreochromis sp.) in the Ping River,Northern Thailand     

Guoyi Niu  Dilok Wongsathein  Sukolrat Boonyayatra  Rutch Khattiya 《Aquaculture Research》2019,50(12):3643-3652

Edwardsiella tarda is a pathogen that causes edwardsiellosis in aquatic animals. The emergence of multiple antibiotic‐resistant strains makes antibiotic treatment difficult. This study aimed to investigate the antibiotic susceptibility patterns and the genotypic characterization of E. tarda isolated from cage‐cultured red tilapia in Thailand. A total of 30 isolates were identified as E. tarda using biochemical and molecular analysis. The disc diffusion method for testing antibiotic susceptibility showed all the isolates were resistant to colistin sulphate and oxolinic acid. High levels of resistance to amoxicillin, ampicillin, ceftazidime, oxytetracycline and sulphamethoxazole/trimethoprim were observed as well. The multiple antibiotic resistance index ranged from 0.25 to 0.92, indicating that these isolates had been exposed to high risk sources of contamination where antibiotics were commonly used. All the isolates carried the bla_TEM gene based on polymerase chain reaction (PCR). The tetA and sul3 genes were detected in 90% (27/30) and 26.7% (8/30) of the isolates respectively. Nine different genetic groups of isolates were obtained using enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC‐PCR). A correlation between genetic types and multiple antibiotic‐resistant patterns was found. These results highlight the potential risks of multiple antibiotic‐resistant isolates for humans and the environment.  相似文献   

海洋重金属污染的研究进展   总被引：5，自引：0，他引：5  

田金  李超  宛立  杜牛  赵海勃 《水产科学》2009,28(7)

随着社会文明的发展,海洋污染已成为全球关注的问题.由于沿海城市的发展与工业化进程,工农业和生活污水的不断排入,造成近岸海域严重污染,导致了近岸海域的环境质量下降[1-2].  相似文献   

农机新燃料生物柴油的特性及对环境的影响   总被引：1，自引：0，他引：1  

刘蕾  牛俊玲  姜灵彦 《农机化研究》2009,31(4)

生物燃料是以生物质为载体的能源,直接或间接来源于植物的光合作用,是可替代石化柴油的一种可再生能源,现已开始应用于农业机械领域.为此,阐述了生物柴油的特性,重点分析了生物柴油在生产及农机使用过程中产生的污染成分及对环境的影响,通过与传统的石化柴油作对比,指出了生物柴油作为农机新燃料的优势,同时也提出了许多亟待解决的问题.  相似文献