Identification of an Avirulence Gene in the Fungus Magnaporthe grisea Corresponding to a Resistance Gene at the Pik Locus     

Yasuda N  Noguchi MT  Fujita Y 《Phytopathology》2005,95(7):768-772

ABSTRACT A rice isolate of Magnaporthe grisea collected from China was avirulent on rice cvs. Hattan 3 and 13 other Japanese rice cultivars. The rice cv. Hattan 3 is susceptible to almost all Japanese blast fungus isolates from rice. The genetic basis of avirulence in the Chinese isolate on Japanese rice cultivars was studied using a cross between the Chinese isolate and a laboratory isolate. The segregation of avirulence or virulence was studied in 185 progeny from the cross, and monogenic control was demonstrated for avirulence to the 14 rice cultivars. The resistance gene that corresponds to the avirulence gene (Avr-Hattan 3) is thought to be located at the Pik locus. Resistance and susceptibility in response to the Chinese isolate in F(3) lines of a cross of resistant and susceptible rice cultivars were very similar to the Pik tester isolate, Ken54-20. Random amplified polymorphic DNA markers and restriction fragment length polymorphism markers from genetic maps of the fungus were used to construct a partial genetic map of Avr-Hattan 3. We obtained several flanking markers and one co-segregated marker of Avr-Hattan 3 in the 144 mapping population.  相似文献   

Plasma concentrations of inhibin A in cattle with follicular cysts: relationships with turnover of follicular waves and plasma levels of gonadotropins and steroid hormones     

Todoroki J  Noguchi J  Kikuchi K  Ohnuma K  Ozawa M  Kaneko H 《Domestic animal endocrinology》2004,27(4):333-344

We investigated the profiles of circulating levels of inhibin A and total inhibin in beef cows with follicular cysts in relation to the patterns of follicular development and circulating gonadotropins and steroid hormones. Turnover of follicular waves was monitored in five cows every 2 days for 70 days from 10 days after detection of estrus without ovulation. The mean interwave intervals were 19.6 ± 1.0 days (n = 18 waves with cysts from the five cows). Circulating levels of inhibin A were approximately 170 pg/ml before emergence of follicular waves with cysts and increased (P < 0.05) concomitantly with follicle emergence. High concentrations of inhibin A (greater than 300 pg/ml) were noted for 7 days during the growth phase of cystic follicles, but inhibin A levels decreased gradually when development of the cysts reached a plateau. This profile of inhibin A was similar to those of total inhibin and estradiol, but was inversely related to the changes in plasma FSH concentrations. LH pulse frequency and mean concentrations of LH in cows with cysts were higher than those observed in the luteal phase of normal cyclic cows. These results indicate that the capacity to secrete inhibin, as well as estradiol, is maintained in cystic follicles, the growth of which is extended by LH secretion at levels greater than those seen in the normal luteal phase. Inhibin A plays an important role in the extension of interwave intervals by suppressing recruitment of a new cohort of follicles.  相似文献   

Transcatheter closure of congenital ventricular septal defects in 3 dogs with a detachable coil   总被引：3，自引：0，他引：3  

Fujii Y  Fukuda T  Machida N  Yamane T  Wakao Y 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(6):911-914

The purpose of this study was to evaluate the feasibility, safety, and efficacy of transcatheter closure in dogs with a congenital perimembranous ventricular septal defect (VSD) by using a detachable coil. No dogs showed any symptoms, and results of chest X-rays and ECGs were normal. The diameters of VSD ranged from 2 to 4 mm on echocardiogram. The defect was 2-2.5 mm from the aortic valve. A detachable coil (size 5 mm with 5 loops) designed for patent ductus arteriosus was delivered via the transarterial route. The device was successfully employed in all dogs. A minimal residual shunt was observed in all dogs even though Qp/Qs decreased. Hemolysis and a rate-dependent right-bundle branch block were observed in 1 dog, but there was no clinical significance. No major complication was noted. Pathologic examination after 1 year revealed that the coils were covered with tissue without significant damage to the His-Purkinje conduction system. In conclusion, transcatheter closure of a small perimembranous VSD with a detachable coil can be achieved without major complications or significant pathologic damage at the lesion site.  相似文献   

Molecular characterization of Theileria orientalis piroplasm protein encoded by an open reading frame (To ORF2) in a genomic fragment     

Kim JY  Yokoyama N  Kumar S  Inoue N  Fujisaki K  Sugimoto C 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(8):957-963

In the present study, a novel antigenic protein expressed in the piroplasm stage of Theileria orientalis was characterized. A 4,707 bp genomic fragment amplified by PCR contained two open reading frames (ORFs). The deduced amino acid sequence of the first ORF showed significantly high similarlity to the ubiquitin carboxy terminal hydrolases/proteases while the second ORF (To ORF2) showed homology to several surface antigens of plasmodia. To ORF2 was expressed to determine whether the protein product is expressed by the parasite. In western blot analysis, bovine antiserum from a T. orientalis-infected calf recognized the recombinant protein containing a C-terminal part of the ORF expressed by baculovirus system. Western blot analysis with the anti-To ORF2 mouse serum recognized a 48 kDa protein in T. orientalis piroplasm lysates. Indirect immunofluorescence antibody test by confocal scanning laser microscopic analysis showed that antisera against the recombinant protein recognized T. orientalis piroplasm in the infected erythrocyte. The results from this study indicate that To ORF2 protein is expressed at the piroplasm stage and is immunogenic. This novel antigenic To ORF2 protein could be exploited for vaccine development against bovine piroplasmosis.  相似文献   

Retrospective analysis of the efficacy of controlled internal drug release in follicular cysts in an embryo donor beef herd     

Todoroki J  Noguchi J  Kikuchi K  Kaneko H 《The Journal of reproduction and development》2004,50(3):369-373

We investigated the efficacy of a controlled internal drug release (CIDR) device in the reproductive management of an embryo donor beef herd. Superovulation of embryo donors was routinely induced by a combination of FSH and prostaglandin F(2)alpha analogue (PGF(2)alpha) at intervals of a few months, and after embryo recovery estrus of the donors was synchronized with PGF2(2)alpha. Between 1996 and 1998, approximately 20% of donors were diagnosed as having follicular cysts every year. Twenty-eight cases of follicular cysts recorded between 1997 and 1998 were treated with a CIDR device for 14 days to evaluate the efficacy of CIDR in resolving follicular cysts in donor herds. Initial recovery was defined as the occurrence of ovulation with estrous behavior and subsequent formation of a corpus luteum after removal of the CIDR. Initial recovery was recognized in all cases (n=28). Follicular cysts did not recur after repeated embryo recovery in 64% of the initially recovered donors, but in 36% of affected donors cysts recurred after the next embryo recovery. Subsequently, with a CIDR device instead of PGF(2)alpha, we synchronized estrus after embryo recovery in the same herd from 1999 to 2000, to investigate the ability of CIDR to prevent the initiation of follicular cysts. Of the donors used between 1999 and 2000, approximately 30% had a history of follicular cysts. Use of CIDR for estrous synchronization after embryo recovery lowered the incidence of follicular cysts to 3% in 1999 and 0% in 2000. Treatment with CIDR proved effective at resolving follicular cysts in the embryo donor beef herd and enabled re-use of donors affected with follicular cysts. CIDR is also likely to be efficacious in lowering the occurrence of follicular cysts in donor herds when it is used for estrous synchronization after embryo recovery.  相似文献   

Reduced Efficacy of Ivermectin Treatments in Gastrointestinal Nematode Infections of Grazing Cattle in Japan     

Noboru KUDO  Toshiro YOSHIOKA  Yasushi WATANABE  Yuki TERAZONO  Shino TAKENOUCHI  Takuto DONOMOTO  Kensuke NAKAJIMA  Kaori HITOSUGI  Ryusuke TSUKADA  Hiromi IKADAI  Takashi OYAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(11):1487-1491

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Molecular basis of the shish-kebab morphology in polymer crystallization     

Kimata S  Sakurai T  Nozue Y  Kasahara T  Yamaguchi N  Karino T  Shibayama M  Kornfield JA 《Science (New York, N.Y.)》2007,316(5827):1014-1017

In the rich and long-standing literature on the flow-induced formation of oriented precursors to polymer crystallization, it is often asserted that the longest, most extended chains are the dominant molecular species in the "shish" of the "shish-kebab" formation. We performed a critical examination of this widely held view, using deuterium labeling to distinguish different chain lengths within an overall distribution. Small-angle neutron-scattering patterns of the differently labeled materials showed that long chains are not overrepresented in the shish relative to their concentration in the material as a whole. We observed that the longest chains play a catalytic role, recruiting other chains adjacent to them into formation of the shish.  相似文献   

Itokawa dust particles: a direct link between S-type asteroids and ordinary chondrites     

Nakamura T  Noguchi T  Tanaka M  Zolensky ME  Kimura M  Tsuchiyama A  Nakato A  Ogami T  Ishida H  Uesugi M  Yada T  Shirai K  Fujimura A  Okazaki R  Sandford SA  Ishibashi Y  Abe M  Okada T  Ueno M  Mukai T  Yoshikawa M  Kawaguchi J 《Science (New York, N.Y.)》2011,333(6046):1113-1116

The Hayabusa spacecraft successfully recovered dust particles from the surface of near-Earth asteroid 25143 Itokawa. Synchrotron-radiation x-ray diffraction and transmission and scanning electron microscope analyses indicate that the mineralogy and mineral chemistry of the Itokawa dust particles are identical to those of thermally metamorphosed LL chondrites, consistent with spectroscopic observations made from Earth and by the Hayabusa spacecraft. Our results directly demonstrate that ordinary chondrites, the most abundant meteorites found on Earth, come from S-type asteroids. Mineral chemistry indicates that the majority of regolith surface particles suffered long-term thermal annealing and subsequent impact shock, suggesting that Itokawa is an asteroid made of reassembled pieces of the interior portions of a once larger asteroid.  相似文献   

Urinary free metanephrines measurement in dogs with adrenal gland diseases using a new simple liquid chromatography tandem mass spectrometry method     

Noboru SASAKI  Yoshinori IKENAKA  Yumiko INOUE  Takahiro ICHISE  Noriyuki NAGATA  Mayumi ISHIZUKA  Syouta MM NAKAYAMA  Kensuke NAKAMURA  Mitsuyoshi TAKIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):648

Measurement of urinary metanephrines in spot samples is used for the diagnosis of canine pheochromocytoma (PC). We describe a simple analytical method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for measuring free metanephrine (MN) and normetanephrine (NMN) in spot urine samples. Using the developed method, we evaluated the stability of urinary free-MN and free-NMN at various storing conditions. In addition, we assessed the feasibility of urinary free-MN and -NMN measurement for diagnosing PC. Urine samples were mixed with stable isotope internal standards and thereafter purified by ultrafiltration. The purified samples were analyzed by LC-MS/MS in the multiple reaction monitoring mode after separation on a multimode octa decyl silyl column. The coefficient of variation of free-MN and -NMN measurement was 7.6% and 5.5%, respectively. The linearity range was 0.5–10 µg/l for both analytes. Degradation was less than 10% for both analytes under any of the storage conditions. The median free-NMN ratio to creatinine of 9 PC dogs (595, range 144–47,961) was significantly higher (P<0.05) than that of 13 dogs with hypercortisolism (125, range 52–224) or 15 healthy dogs (85, range 50–117). The developed method is simple and may not require acidification of spot urine. The results of this preliminary retrospective study suggest that the measurement of urinary free metanephrines is a promising tool for diagnosing canine PC.  相似文献   

Potential for Using Plant Xylem Sap to Evaluate Inorganic Nutrient Availability in Soil     

Akira Noguchi  Masakatsu Kageyama  Fumie Shinmachi  Urs Schmidhalter  Isao Hasegawa 《Soil Science and Plant Nutrition》2005,51(3):343-350

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