Increased collagen III in culled chicken meat after feeding dietary wood charcoal and vinegar contributes to palatability and tenderness     

Kohsyo Yamauchi  Noboru Manabe  Yoshiki Matsumoto  Shin‐ichi Takenoyama  Koh‐en Yamauchi 《Animal Science Journal》2014,85(4):468-480

We comprehensively evaluated meat quality in chickens fed a diet consisting of wood charcoal and vinegar (WCV) using food scientific and histological approaches. In culled hens, lipid and fatty acid in Musculus semimembranosus, cooking loss and sensory tests of whole thigh meat, and meat texture of breast meat were observed. In male broilers, cross section of M. semimembranosus was used for observations on muscle area, perimysium, non‐collagen total protein and total collagen content, and anti‐collagen I and III reactions. In frozen male broilers, conventional morphology of M. semimembranosus as well as chicken anti‐collagen III reaction to selected muscles of thigh meat and breast meat were compared between the control and WCV‐fed birds. Increased lipid and fatty acids, decreased cooking loss, high score in total evaluation for sensory test of thigh meat, and decreased meat texture values were observed for culled hens fed WCV. The higher values of muscle area, total collagen and collagen III were observed for broilers fed WCV. No perimysium collapse for M. semitendinosus or increased collagen III reactions of M. tensor fasciae latae, the flexor muscle group and M. pectoralis superficialis were observed for frozen muscles in the WCV group. These total results suggest that WCV produces palatable and tender meat by increasing collagen III.  相似文献   

Phosphorylation and growth inhibitory activity of all ketohexose analogs     

Hisashi Kato‐Noguchi  Kozue Okada 《Weed Biology and Management》2013,13(3):114-118

Sugars are not only important energy sources and structural components, but they also act as signaling molecules that are involved in specific signal sensing. Among all ketohexose analogs (d ‐fructose, d ‐psicose, d ‐tagatose, d ‐sorbose, l ‐fructose, l ‐psicose, l ‐tagatose and l ‐sorbose), only d ‐psicose inhibited the growth of Arabidopsis roots. Phosphorylation by fructokinase occurred in d ‐fructose and d ‐psicose. d ‐Psicose‐induced inhibition was relieved by adding d ‐fructose. Thus, the inhibition could not be attributed to the toxicity of phosphorylated d ‐psicose. The phosphorylation process requires ATP. After phosphorylation, d ‐fructose is metabolized in glycolysis and becomes energy sources and structural components, whereas d ‐psicose cannot contribute to the energy sources and structural components because it does not get metabolized further. However, d ‐psicose did not affect ATP level in the Arabidopsis roots, suggesting that the d ‐psicose‐induced growth inhibition may not be related to the starvation of ATP. The phosphorylation of ketohexoses by fructokinase is known to a trigger signal‐sensing resulting in growth inhibition. Therefore, d ‐psicose can be phosphorylated by fructokinase and this process may play a possible role in signal‐sensing. This is probably one of the useful model systems for the study of the hexokinase‐independent sugar‐sensing function and for developing new types of weed‐control agents.  相似文献   

Saving Genetic Resources of Native Pigs in Occidental and Oriental Countries — Practical Examples of the Characterization and Utilization of Native Pigs in Hungary and Laos     

Jozsef RáTKY  Istvan EGERSZEGI  Peter TOTH  Soukanh KEONUCHAN  Takashi NAGAI  Kazuhiro KIKUCHI  Noboru MANABE  Klaus-Peter BRüSSOW 《The Journal of reproduction and development》2013,59(5):437-441

Worldwide, only a few “fatty” pig breeds exist with different and/or regional utilization. Using the Hungarian Mangalica, which almost went extinct in Europe and the Lao Moo Lat pig, which still has a large population in South-East Asia as exemples, we wanted to demonstrate that indigenous (fatty) pig breeds may represent both national value and tremendous economic potential. Since these less prolific and less productive breeds cannot contribute to mass production, new market roles and methods should be established for them in the premium segment of pork trading. Thus their preservation and propagation needs the comprehensive collaboration of commercial, governmental actors and researchers. Briefly summarizing the history, we report the current results of reproductive physiology research. The commercial renaissance of Mangalica pigs is indebted to the enthusiastic efforts of basic scientists, pig breeding experts and dedicated Mangalica producers. Scientific achievements were applied to practical breeding and production of delicious pork and processed products, which ultimately made the economic success in the Mangalica sector possible. Both, research on and utilization of endangered (pig) breeds maintain not only breed diversities, but also may improve the livelihood of farmers worldwide.  相似文献   

PCR Detection and Genetic Diversity of Bovine Hemoprotozoan Parasites in Vietnam     

Thillaiampalam SIVAKUMAR  Dinh Thi Bich LAN  Phung Thang LONG  Takeshi YOSHINARI  Muncharee TATTIYAPONG  Azirwan GUSWANTO  Kazuhiro OKUBO  Ikuo IGARASHI  Noboru INOUE  Xuenan XUAN  Naoaki YOKOYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(11):1455-1462

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In vitro antiseptic susceptibilities for Staphylococcus pseudintermedius isolated from canine superficial pyoderma in Japan     

Nobuo Murayama  Masahiko Nagata  Yuri Terada  Mio Okuaki  Noriyuki Takemura  Hidemasa Nakaminami  Norihisa Noguchi 《Veterinary dermatology》2013,24(1):126-e29

Background –  Topical therapy, particularly with chlorhexidine, is becoming increasingly common as a treatment option for canine pyoderma; however, there are limited studies on the susceptibility of Staphylococcus pseudintermedius to chlorhexidine compounds. Objectives –  To determine the in vitro susceptibility of both meticillin‐resistant and meticillin‐susceptible S. pseudintermedius isolates to chlorhexidine and other antiseptic agents and the presence of multidrug efflux pump genes. Samples –  One hundred S. pseudintermedius isolates from 23 initial and 77 recurrent cases of canine pyoderma. Methods –  After bacterial identification and mecA testing, minimal inhibitory concentrations (MICs) of antiseptic agents were determined. Multidrug efflux pump genes, including qacA, qacB and smr, were identified. Results –  Of the 100 isolates, 57 were identified as meticillin‐resistant S. pseudintermedius. The MIC₉₀ of chlorhexidine acetate, chlorhexidine gluconate, acriflavine, ethidium bromide and benzalkonium chloride were 1, 1, 2, 0.5 and 2 μg/mL, respectively. Multidrug efflux pump genes qacA, qacB and smr were not detected in any of the isolates. Conclusions and clinical importance –  The MICs for chlorhexidine and other antiseptics remain low, and multidrug efflux pump genes were not found in the tested isolates.  相似文献   

家兔细胞核移植技术研究     

常万存  韩建永  马世援  张红卫  陈忠科  Noboru Fujihar  王庆国  刘文浩 《西北农业学报》2002,11(1):17-19

以家兔16－32细胞期胚胎卵裂为核供体，显微注射到去核卵母细胞周隙中，共制作完成229枚重组胚，2次电融合后率达到76．8％。重组胚与兔胚成胎成纤维细胞共同培养，卵裂率为65．7％（23／35），桑囊率达到22．9％（8／35），均显著高于（P＜0．01）微滴直接培养结果（43．3％、13／30；16．7％，5／30）。将111枚重组胚手术移植到9只同期发情的受体母兔输卵管中，2只妊娠，其中1只完成足月胚胎发育产出克隆仔兔2只。  相似文献   

Autophagy-dependent viral recognition by plasmacytoid dendritic cells     

Lee HK  Lund JM  Ramanathan B  Mizushima N  Iwasaki A 《Science (New York, N.Y.)》2007,315(5817):1398-1401

Plasmacytoid dendritic cells (pDCs) detect viruses in the acidified endosomes by means of Toll-like receptors (TLRs). Yet, pDC responses to certain single-stranded RNA (ssRNA) viruses occur only after live viral infection. We present evidence here that the recognition of such viruses by TLR7 requires transport of cytosolic viral replication intermediates into the lysosome by the process of autophagy. In addition, autophagy was found to be required for the production of interferon-alpha by pDCs. These results support a key role for autophagy in mediating ssRNA virus detection and interferon-alpha secretion by pDCs and suggest that cytosolic replication intermediates of viruses serve as pathogen signatures recognized by TLR7.  相似文献   

THE DIFFERENTIATION OF HERPETOMONADS AND LEISHMANIAS BY BIOLOGICAL TESTS     

Noguchi H 《Science (New York, N.Y.)》1926,63(1637):503-504

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Chondrulelike objects in short-period comet 81P/Wild 2   总被引：1，自引：0，他引：1  

Nakamura T  Noguchi T  Tsuchiyama A  Ushikubo T  Kita NT  Valley JW  Zolensky ME  Kakazu Y  Sakamoto K  Mashio E  Uesugi K  Nakano T 《Science (New York, N.Y.)》2008,321(5896):1664-1667

The Stardust spacecraft returned cometary samples that contain crystalline material, but the origin of the material is not yet well understood. We found four crystalline particles from comet 81P/Wild 2 that were apparently formed by flash-melting at a high temperature and are texturally, mineralogically, and compositionally similar to chondrules. Chondrules are submillimeter particles that dominate chondrites and are believed to have formed in the inner solar nebula. The comet particles show oxygen isotope compositions similar to chondrules in carbonaceous chondrites that compose the middle-to-outer asteroid belt. The presence of the chondrulelike objects in the comet suggests that chondrules have been transported out to the cold outer solar nebula and spread widely over the early solar system.  相似文献   

Investigation of serum concentrations and immunohistochemical localization of α1-acid glycoprotein in tumor dogs     

Yuki M  Machida N  Sawano T  Itoh H 《Veterinary research communications》2011,35(1):1-11

The purpose of this study was to measure the dynamics of serum α1-acid glycoprotein (AGP) concentration in dogs with various tumors, and to investigate the localization of AGP in some tissues using immunohistochemical staining. Sera were obtained from 171 dogs bearing tumors of various types. Serum AGP concentration was measured by single radial immunodiffusion. Tumors occurring in the liver and spleen were also investigated immunohistochemically using anti-canine AGP antibody. Mean serum AGP levels were 749 ± 602 mg/L in dogs with carcinoma (n = 39), 1,014 ± 971 mg/L with sarcoma (n = 18), and 887 ± 935 mg/L with round cell tumors (n = 46), all significantly higher than serum AGP level in healthy dogs (n = 137, 364 ± 106 mg/L). Mean serum AGP levels were significantly higher than in healthy dogs in complex mammary gland carcinoma (n = 5, 876 ± 721 mg/L), malignant melanoma (n = 7, 1,010 ± 821 mg/L), and hepatocellular carcinoma (n = 5, 936 ± 741 mg/L) among carcinomas, hemangiosarcoma (n = 5, 1,740 ± 1,323 mg/L) among sarcomas, and lymphoma (n = 19, 1,072 ± 965 mg/L) and histiocytic tumor (n = 6, 1,800 ± 1,387 mg/L) among round cell tumors. In an immunohistochemical investigation of AGP localization, both weak and strong staining for anti-AGP antibody were seen in hepatic tissue in dogs with primary non-tumorous lesions originating in the spleen (hematoma) and elevated serum AGP, but all tumor tissue in the spleen was negative. Among dogs with primary tumor lesions of the spleen (hemangiosarcoma) and elevated serum AGP levels, both weak, moderate and strong staining for anti-AGP antibody were seen in hepatic tissue, while strong positive staining was apparent in all tumorous tissue from the spleen. In primary tumor lesions in the liver (hepatocellular carcinoma), both moderate and strong staining for anti-AGP antibody were seen in normal hepatic tissue, and both weak, moderate and strong staining were seen in tumor tissues of the liver. AGP levels thus appear to be elevated in dogs with carcinomas, sarcomas, and round cell tumors. With some of these malignant tumors, localization of AGP in tumor tissue was seen.  相似文献