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Antibodies to Asp-Asp-Glu-Asp can inhibit transport of nuclear proteins into the nucleus 总被引：18，自引：0，他引：18
Y Yoneda N Imamoto-Sonobe Y Matsuoka R Iwamoto Y Kiho T Uchida 《Science (New York, N.Y.)》1988,242(4876):275-278
The signal sequence of simian virus 40 (SV40) large T-antigen for translocation into the nucleus is composed of positively charged amino acids Lys-Lys-Lys-Arg-Lys. Rabbit antibodies to a synthetic peptide containing the negatively charged amino acid sequence Asp-Asp-Asp-Glu-Asp were obtained. Indirect immunofluorescence of the antigens recognized by the antibody was punctate at the nuclear rim or the nuclear surface, depending on the plane of focus. The antibody blocked transport of nuclear proteins into the nucleus. The antigens recognized by the antibody were predominantly localized to the nuclear pores. 相似文献
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Humitake Seki Nobuharu Aoshima F. A. Whitney C. S. Wong 《Water, air, and soil pollution》1981,16(3):331-337
Influences of energy and particulate matter were experimentally reduced by eliminating the upper part of the euphotic zone, using a large sediment-seawater enclosure (SSE)in situ in Saanich Inlet, B.C., Canada. The standing stock of heterotrophic microorganisms, as determined by ATP concentration, in the experimental dysphotic zone was not appreciably affected, although the decreased supply of organic material did reduce their rate of nutrient uptake from the environment. In the mesotrophic level of the natural dysphotic zone, the turnover rate of glutamic acid remained near the initial rate by readjusting into a new balance. 相似文献
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Tomohito Arao Tomoyuki Makino Akira Kawasaki Ikuko Akahane Nobuharu Kiho 《Soil Science and Plant Nutrition》2018,64(3):433-437
ABSTRACTHigh air temperatures during the grain-filling stage of rice (Oryza sativa L.) decrease grain yield and quality. Temperatures above the optimal growth temperature impair dry matter production due to reduced grain size. Since the arsenic (As) concentration in grain is determined by the As accumulation and the grain weight, we focused on the effect of air temperature before and after rice heading on the As concentration in grain. Rice plants have been planted in the same field every year since 1974 with a similar set-up and water management. We analyzed the As concentrations in stored rice grains produced in 1995–2014 and evaluated the possible relationships with the meteorological data for the associated production year. The average daily mean air temperatures (DMTs) from 2 weeks after the heading day to 4 weeks after the heading day were significantly correlated with the inorganic As concentrations in the grains, whereas there was no significant correlation between the grain As concentration and the average DMT during 1 week after the heading day or 3 weeks before the heading day. These findings indicate that high air temperature is more effective at increasing As in grain in the late ripening stage of rice than before the ripening stage or in the early ripening stage. Further experiments are necessary to clarify why As concentrations in grain increase when high air temperatures occur in the late ripening stage of rice. 相似文献
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In the present study, a plate and a doweled fastener made of compressed wood (CW) were newly introduced into a moment resisting
column-beam joint system for a small portal frame structure. A mechanical model that contains not only an axial spring, but
also a rotational spring, considered resistant factors to verify how each element resists rotation. Theoretical performance
was compared with experimental data. Consequently, the mechanical model was shown to be suitable and the combination of resisting
factors was found to be very effective; i.e., the rotational spring provides more influence on the stiffness and moment compared
with the axial spring. Large moment and ductility can be achieved by virtue of the high embedding performance of the CW plate
in the rotational spring, accompanied with the high shearing performance of the CW dowel in the axial spring. 相似文献
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