Corpus Luteum Development and Function after Supplementation of Long‐Acting Progesterone During the Early Luteal Phase in Beef Cattle     

G Pugliesi  ML Oliveria  SC Scolari  E Lopes  FV Pinaffi  BT Miagawa  YN Paiva  JRG Maio  GP Nogueira  M Binelli 《Reproduction in domestic animals》2014,49(1):85-91

Strategic supplementation of P4 may be used to increase conception rates in cattle, but timing of supplementation in relation to ovulation, mass of supplementary P4 and formulation of the P4‐containing supplement has not been determined for beef cattle. Effects of supplementation of long‐acting progesterone (P4) on Days 2 or 3 post‐ovulation on development, function and regression of corpus luteum (CL) were studied in beef cattle. Cows were synchronized with an oestradiol/P4‐based protocol and treated with 150 or 300 mg of long‐acting P4 on Day 2 or 3 post‐ovulation (6–7 cows/group). Colour‐doppler ultrasound scanning and blood sample collection were performed from Day 2–21.5. Plasma P4 concentrations were greater (p < 0.05) from Day 2.5–5.5 in the Day 2‐treated groups and from Day 3.5–5.5 in the Day 3‐treated cows than in the control group. CL area and blood flow during Day 2–8.5 did not differ (p > 0.05) among groups, suggesting no effect of P4 treatment on luteal development. The frequency of cows that began luteolysis before Day 15 was greater (p < 0.04) in cows treated with 300 mg than in the controls, but there were no differences between non‐treated and 150 mg‐treated cows. The interval from pre‐treatment ovulation to functional and structural luteolysis was shorter (p < 0.01) in the combined P4‐treated groups than in the control cows. In conclusion, was showed for the first time that long‐acting P4 supplementation on Day 2 or 3 post‐ovulation increases P4 concentrations for ≥3 day, has no effect on luteal development, but anticipates the beginning of luteolysis in beef cattle.  相似文献   

Sorption-desorption isotherms and biodegradation of glyphosate in two tropical soils aged with eucalyptus biochar     

Leonardo Vilela Junqueira  Rodrigo Nogueira de Sousa  Cassio de Souza Almeida  Felipe Gimenes Alonso  Valdemar Luiz Tornisielo 《Archives of Agronomy and Soil Science》2020,66(12):1651-1667

ABSTRACT

The objective of this study was to evaluate the sorption-desorption process and biodegradation of glyphosate in two tropical soils aged with biochar derived from eucalyptus. The biochar aging period was 30 d. There was little difference between the amounts of sorbed glyphosate in Ultisol (96.8, 96.8 and 96.4%) and Alfisol (97.1, 97.5 and 97.4%) soils that were unamended or amended with biochar aged for 0 or 30 d, respectively. Similar amounts of desorbed herbicide occurred in Ultisol (3.3, 3.3 and 3.4%) and Alfisol (4.1, 4.2 and 3.9%) soils, respectively. The degradation time half-life (DT50) of glyphosate in Ultisol unamended and initial amended were higher (38 and 36 d, respectively) than DT50 in the amended soil with 30 d of biochar aging (27 d); and in the Alfisol DT50 was higher in unamended soil (38 d), and similar in soil unamended at 0 and 30 d of biochar aging (21 and 26 d, respectively). The addition of biochar to two tropical soils or its aging did not have any effect on the sorption and desorption of glyphosate and its biodegradation in relation to the unamended soils, and it can did not affect the transport and persistence of this herbicide in soil.  相似文献   

Microwave-assisted Acid decomposition of animal- and plant-derived samples for element analysis     

Carrilho EN  Gonzalez MH  Nogueira AR  Cruz GM  Nóbrega JA 《Journal of agricultural and food chemistry》2002,50(15):4164-4168

A single microwave-assisted acid-decomposition procedure is proposed for sample preparation of plant- and bovine-derived materials prior to multielement determination by inductively coupled plasma-optical emission spectrometry (ICP-OES). The procedure involved sample grinding in a cryogenic mill to reduce particle sizes and increase the surface area for acid attack, followed by decomposition under high pressure and temperature. A single heating program was employed and efficiently destroyed most of the organic matrix by using small volumes of nitric acid and hydrogen peroxide. Accuracy of the elemental analysis was indicated by the good agreement between certified and found values of elements in plant and animal samples at 95% confidence level. The sample preparation procedure was fast and reproducible, and proved to be efficient for elemental analysis in agricultural and food-related samples by a sensitive multielement technique such as ICP-OES.  相似文献   

Shading effect on physiological parameters and in vitro embryo production of tropical adapted Nellore heifers in integrated crop-livestock-forest systems     

Leite da Silva  Wilian Aparecido  Poehland  Ralf  Carvalho de Oliveira  Caroline  Ribeiro Ferreira  Mariane Gabriela Cesar  Garcia de Almeida  Ricardo  Cáceres  Mirela Brochado Souza  Macedo  Gustavo Guerino  da Costa e Silva  Eliane Vianna  Alves  Fabiana Villa  Nogueira  Eriklis  de Andrade Melo-Sterza  Fabiana 《Tropical animal health and production》2020,52(5):2273-2281

Tropical Animal Health and Production - The aim of this study was to evaluate the impact of increased shadow supply in integrated crop-livestock-forest systems on in vitro embryonic development and...  相似文献   

Analytical characterization of madeira wine     

Nogueira JM  Nascimento AM 《Journal of agricultural and food chemistry》1999,47(2):566-575

For a correct characterization of Madeira wine commercially available in the market, 52 samples having different types and ages, representative of the delimited region of Madeira (liquor wine of quality produced in delimited region), were analyzed in relation to physicochemical and sensorial parameters. Standard methodology for spirits and alcoholic beverages as well as a council of tasters were adopted respectively to quantify such parameters, according to European Union regulations. The main physicochemical parameters analyzed demonstrated that Madeira wine represent a high quality beverage without toxicological risks, as all constituents have in general contents clearly below the maximum concentration admissible by the national and/or international rules. A reasonable differentiation of properties could be achieved between samples having different types and ages, when principal component, discriminant and cluster analyses were applied to the analytical data, especially for physicochemical parameters.  相似文献   

Necrotic enteritis in collared (Pecari tajacu) and white-lipped (Tayassu pecari) peccaries     

de Carvalho MA  Santos PS  Nogueira SS  Lessa F  Almeida Vdos A  Le?a-Júnior NF  Salvarani FM  Lobato FC  Nogueira-Filho SL 《Journal of zoo and wildlife medicine》2011,42(4):732-734

An outbreak of necrotic enteritis caused by Clostridium perfringens type C was diagnosed in captive collared (Pecari tajacu) and white-lipped (Tayassu pecari) peccaries housed in the Laboratory of Applied Ethology of Universidade Estadual de Santa Cruz located in Ilhéus, State of Bahia, Brazil. Four collared peccaries and three white-lipped peccaries, all juveniles (25-105 days old), were affected. For all affected animals, lethargy and inappetance were followed by sudden death within 24 hours. Histopathology of intestinal wall, culture of C. perfringens type C, and the identification of beta-toxin from intestinal content confirmed the diagnosis.  相似文献   

Bovine abortion caused by Listeria ivanovii     

PA GILL  JG BOULTON  GC FRASER  AE STEVENSON  LA REDDACLIFF 《Australian veterinary journal》1997,75(3):214-214

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Uterine Responses to Exogenous Oxytocin Before and After Pre-partum Luteolysis in the Cow     

MAM Taverne  NCM de Schwartz  M Kankofer  MM Bevers  HA van Oord  D Schams  St Gutjahr  & GC van der Weijden 《Reproduction in domestic animals》2001,36(5):267-272

The aim of this study was to test the functional status of uterine oxytocin receptors in cows in vivo around parturition. The animals received consecutive, intra‐arterial injections of 800, 1600 and 3200 mU of oxytocin at three different stages: during late gestation (days 260–274), at 12 h and at 24 h after intramuscular injection of a prostaglandin F_2α analogue at day 275 to induce parturition. Cows (n=6) had been provided with myometrial electrodes and a catheter had been installed in the aorta and in a branch of the uterine vein (UV). Regular blood samples were obtained from the UV from 5 min before until 45 min after each oxytocin injection to measure plasma levels of prostaglandin F_2α (PGF_2α) and oxytocin. Uterine electromyographic (EMG) activity was registered continuously during each experiment. The increase of oxytocin levels in UV plasma after intra‐arterial injections was dose dependent (p < 0.02). Pre‐ and post treatment oxytocin levels at 24 h after induction of parturition were significantly increased (p=0.0313). Both during late pregnancy and at 12 h after induction of parturition, oxytocin caused a significant increase in EMG activity (p=0.022). After the 3200 mU dose the increase was significantly higher than with the other 2 doses (p=0.004). After each dose, EMG activity returned to baseline levels within some 15 min. At 24 h after induction of parturition, the pre‐treatment level of EMG activity had increased. Doses of 800 mU and 1600 mU of oxytocin produced a significant (p=0.022) increment of EMG activity, which was of the same magnitude as during the preceding stages; after 3200 mU of oxytocin the response was significantly higher than before (p=0.008). No significant increases of PGF_2α levels in UV plasma could be measured after oxytocin injections at any of the three stages. It is concluded that the myometrium of the pregnant cow responds in vivo to physiological doses of oxytocin. At 24 h after induction of parturition, when luteolysis has occurred and a parturient pattern of parturient myometrial activity has already started to develop, the response is enhanced. Physiological doses of oxytocin did not evoke a spurt release of PGF_2α in uterine venous blood during the peripartal period.  相似文献   

Effects of bovine leukemia virus infection on milk neutrophil function and the milk lymphocyte profile     

Alice Maria Melville Paiva Della Libera  Fernando Nogueira de Souza  Camila Freitas Batista  Bruna Parapinski Santos  Luis Fernando Fernandes de Azevedo  Eduardo Milton Ramos Sanchez  Soraia Araújo Diniz  Marcos Xavier Silva  Jo?o Paulo Haddad  Maiara Garcia Blagitz 《Veterinary research》2015,46(1)

The effects of bovine leukemia virus (BLV) on the immune response have been extensively investigated; however, its effects on mammary gland immunity are only speculative. Although BLV has a tropism for B cells, it can affect both adaptive and innate immunities because these systems share many effector mechanisms. This scenario is the basis of this investigation of the effects of BLV on mammary gland immunity, which is largely dependent upon neutrophilic functions. Thus, the present study sought to examine neutrophilic functions and the lymphocyte profile in the milk of naturally BLV-infected cows. The viability of the milk neutrophils and the percentage of milk neutrophils that produced reactive oxygen species (ROS) or phagocytosed Staphylococcus aureus were similar between BLV-infected and BLV-uninfected dairy cows. Furthermore, the expression of CD62L and CD11b by the milk neutrophils and the percentage of milk neutrophils (CH138⁺ cells) that were obtained from the udder quarters of the BLV-infected cows were not altered. Conversely, the median fluorescence intensity (MFI) representing intracellular ROS production and the phagocytosis of S. aureus, the expression of CD44 by the milk neutrophils and the percentage of apoptotic B cells were lower in the milk cells from BLV-infected dairy cows, particularly those from animals with persistent lymphocytosis (PL). The lymphocyte subsets were not different among the groups, with the exception of the percentage of CD5⁻/CD11b⁻ B cells, which was higher in the milk cells from BLV-infected cows, particularly those with PL. Thus, the present study provides novel insight into the implications of BLV infection for mammary gland immunity.  相似文献   

What is the value of eucalyptus monocultures for the biodiversity of the Atlantic forest? A multitaxa study in southern Bahia,Brazil     

Pedro Luís Bernardo da Rocha  Blandina Felipe Viana  Márcio Zikán Cardoso  Amada Mariana Costa de Melo  Misonete Gueidneli Cavalcanti Costa  Rodrigo Nogueira de Vasconcelos  Tatiana Bichara Dantas 《林业研究》2013,24(2):263-272

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