Structure of the Cell-Wall-Associated Polysaccharides from the Deep-Sea Marine Bacterium Devosia submarina KMM 9415T     

Maxim S. Kokoulin  Lyudmila A. Romanenko  Aleksandra S. Kuzmich  Oleg Chernikov 《Marine drugs》2021,19(12)

Two cell-wall-associated polysaccharides were isolated and purified from the deep-sea marine bacterium Devosia submarina KMM 9415^T, purified by ultracentrifugation and enzymatic treatment, separated by chromatographic techniques, and studied by sugar analyses and NMR spectroscopy. The first polysaccharide with a molecular weight of about 20.7 kDa was found to contain d-arabinose, and the following structure of its disaccharide repeating unit was established: →2)-α-d-Araf-(1→5)-α-d-Araf-(1→. The second polysaccharide was shown to consist of d-galactose and a rare component of bacterial glycans-d-xylulose: →3)-α-d-Galp-(1→3)-β-d-Xluf-(1→.  相似文献   

Changes in Phosphate Fractions Extracted From Different Organs of Phosphorus Starved Nitrogen Fixing Pea Plants     

Gergina Emilova Tsvetkova  Georgi Ivanov Georgiev 《Journal of plant nutrition》2013,36(12):2129-2140

The present work investigates the impact of phosphorus (P) starvation on plant growth, symbiotic nitrogen fixation, and internal P status (determined as extracted P fractions) of leaves, roots, and nodules of 27-days–old pea (Pisum sativum L) plants inoculated with Rhizobium leguminosarum bv viciae strain D293. The procedure of separation of organic and inorganic P compounds in 10% perchloric acid (HCLO₄) and the absorption of nucleotides in active charcoal gave several fractions, containing different phosphorus compounds, which were extracted and determined as inorganic phosphate after combustion. These are acid soluble and insoluble P, sugar P, nucleotide P, and inorganic P. The P starvation of plants inhibited significantly plant dry mass accumulation, nodulation rate and specific nitrogenase activity of nodules. These results were accompanied with lower quantities of total P per plant, acid soluble and acid non-soluble P fractions in all plant organs. The inhibited accumulation of P in the acid soluble P fraction was associated with decrease of sugar, nucleotide and inorganic P in all plant organs. The most negatively affected were all P fractions extracted from nodules and leaves. The low content of inorganic P in the stressed plant tissues was regarded as primary reason for induced alterations in the content of analyzed P fractions.  相似文献   

Ripening attributes of new passion fruit line featuring seasonal non-climacteric behavior     

Goldenberg L  Feygenberg O  Samach A  Pesis E 《Journal of agricultural and food chemistry》2012,60(7):1810-1821

The passion fruit hybrid cultivar 'Passion Dream' (PD) produces two cycles of fruiting per year, in the summer and winter. Self-hybridization of PD created various lines, including 'Ripens during Summer' (RS), which lacks the ability to abscise during winter, suggesting a seasonal nonclimacteric behavior. The two lines were characterized by several quality traits: PD produced high ethylene levels in both seasons; RS produced significantly less ethylene during summer and almost none during winter. The ratio of total soluble solids to titratable acidity (TSS/TA), and aroma volatiles production, as determined by solid-phase microextraction/gas chromatograph-mass spectrometer (SPME/GC-MS) techniques, and taste indices were highest in PD summer fruits and lowest in RS winter fruits. Peel color in PD was affected by environmental and storage temperatures, whereas RS fruits always showed a strong purple color. The present findings suggest that ethylene production levels in passion fruit greatly influence various ripening processes, including acid degradation, increased TSS/TA ratio, accumulation of aroma volatiles, and tastiness.  相似文献   

Utilization of diflubenzuron to controlgelechia senticetella (Stgr.) (Lepidoptera: Geiechiidae), a dangerous pest ofjuniperus excelsa M. B. (Cupressaceae) in Bulgaria     

G. Georgiev  G. Tsankov  P. Mirchev 《Anzeiger für Sch?dlingskunde》2000,73(4):107-109

In 1992, experiments were carried out in Bulgaria to controlGelechia senticetella (Stgr.) (Lepidoptera: Geiechiidae), a mining pest on the needles and young shoots ofJuniperus excelsa M. B. by commercial products Dimilin ODC-45 and Dimilin WP-25 based on the insect growth regulator diflubenzuron. Two Ultra Low Volume (ULV) treatments were performed - on March 12^th and April 16^th, during the emerging of the pest larvae out of the infested shoots. Dimilin ODC-45 was applied in a dose of 60-120 ml/ha (27-54 g a.i./ha), dissolved in 5l of diesel oil, and Dimilin WP-25 - in a dose of 200 g/ha (50 g a.i./ha) dissolved in 5l of water. The effect of Dimilin ODC-45 was very high - 92.5-97.6 %. The effect of Dimilin WP-25 was unsatisfactory - 63.4-70.0 %, which was most probably due to the fact that the oil emulsions adhere to the treated plants better than the water solutions, thus ensuring a longer period of action.  相似文献   

Species composition, structure and impact of larval parasitoids of poplar twig borer,Gypsonoma aceriana (Dup.) (Lepidoptera, Tortricidae), on poplar ornamental trees in Sofia     

G. Georgiev  S. Samuelian 《Journal of pest science》1999,72(1):1-4

Studies on the species composition and structure of parasitoid complex of poplar twig borer,Gypsonoma aceriana (Dup.), on poplar ornamental trees in Sofia and their role in reducing the number of this pest have been conducted during the period 1990–1997. Six larval parasitoids—Bracon variator Nees,Orgilus obscurator (Nees),Microdus tumidulus Nees,Apanteles erevanicus Tob. (Braconidae),Pristomerus rufiabdominalis Ucida andPristomerus vulnerator (Panz.) (Ichneumonidae) were identified. Most numerous wereM. tumidulus (41.0%),A. erevanicus (30.6%) andP. rufiabdominalis (19.1%).A. erevanicus andM. tumidulus were dominating during the different years. The parasitoids ofG. aceriana were solitary, asB. variator is an ektoparasite, and the rest—endoparasites. They attack young host larvae and emerge at the end of the pupal stage. The life cycle of parasitoids, that have double generation, are well synchronised with the pest development. This was expressed in the high levels of parasitism, which in some cases reached 61.5%, and as a whole for the different generations they were 5.9–44.4%.A. erevanicus andM. tumidulus were the most important in reducing the number of the pest.  相似文献   

Herbicidal potential of stagonolide, a new phytotoxic nonenolide from Stagonospora cirsii     

Yuzikhin O  Mitina G  Berestetskiy A 《Journal of agricultural and food chemistry》2007,55(19):7707-7711

Stagonospora cirsii is a pathogen of Cirsium arvense, causing necrotic lesions on leaves of this noxious weed. The fungus produced toxic metabolites when grown in liquid culture. A new phytotoxin, named stagonolide, was isolated and characterized as (8R,9R)-8-hydroxy-7-oxo-9-propyl-5-nonen-9-olide by spectroscopic methods. Stagonolide was shown to be a nonhost-specific but selective phytotoxin. Leaves of C. arvense were most sensitive and leaves of tomato and pepper (both Solanaceae) were less sensitive to stagonolide, which was assayed at 5 x 10(-3) M, than other plants. Stagonolide assayed at 5 x 10(-6) M was demonstrated to be a strong inhibitor of root growth in seedlings of C. arvense and some other Asteraceae species. Seedlings growth in wheat and radish was much less affected by the toxin, and seedlings of cucumber were insensitive to it.  相似文献   

Life cycle of the tick Amblyomma parvum Aragão, 1908 (Acari: Ixodidae) and suitability of domestic hosts under laboratory conditions     

Olegário MM  Gerardi M  Tsuruta SA  Szabó MP 《Veterinary parasitology》2011,179(1-3):203-208

Amblyomma parvum is a widespread Neotropical tick found on several domestic animals and is known to harbor a Rickettsia species of yet unknown pathogenicity. However its life cycle on, and suitability of, several of these hosts has not been described. In this work the biology of A. parvum is presented when fed on seven domestic hosts (chicken, dog, rabbit, horse, guinea pig, cattle and goat). The complete life cycle of the tick varied from 96.8 to 102 days. Highest engorgement weight of larvae was from ticks fed on horses and that of nymphs from guinea pigs. Highest larval yield was from guinea pigs and that of nymphs from horses. Engorged female and egg mass weights, yield and conversion of female weight to eggs rates were the highest in dog ticks and the lowest in goat ticks. The highest egg hatching rate was seen in ticks from dogs and the lowest in ticks from cattle. Overall it was seen that dogs were the best host for adult A. parvum ticks, and guinea pigs for immatures. Horses were also shown to be a good host for all tick stages. It can thus be affirmed that A. parvum is a host generalist tick, and its distribution is probably determined by environmental requirements rather than by hosts.  相似文献   

Hydrodefluorination of perfluoroalkyl groups using silylium-carborane catalysts     

Douvris C  Ozerov OV 《Science (New York, N.Y.)》2008,321(5893):1188-1190

Carbon-fluorine bonds are among the most unreactive functionalities in chemistry. Interest in their activation arises in part from the high global warming potentials of anthropogenic polyfluoroorganic compounds. Conversion to carbon-hydrogen bonds (hydrodefluorination) is the simplest modification of carbon-fluorine bonds, but efficient catalytic hydrodefluorination of perfluoroalkyl groups has been an unmet challenge. We report a class of carborane-supported, highly electrophilic silylium compounds that act as long-lived catalysts for hydrodefluorination of trifluoromethyl and nonafluorobutyl groups by widely accessible silanes under mild conditions. The reactions are completely selective for aliphatic carbon-fluorine bonds in preference to aromatic carbon-fluorine bonds.  相似文献   

New parasitoids of Paraphytomyza populi (Kltb.) (Diptera, Agromyzidae) in Bulgaria     

G.Ts. Georgiev  P. Boyadzhiev 《Journal of pest science》2002,75(3):69-71

The parasitoid complex of Paraphytomyza populi (Kltb.) (Diptera: Agromyzidae) was studied in Sofia, Bulgaria in 1997. Poplar leaves with P. populi larvae and pupae were collected from poplar stump shoots and examined in a laboratory. As a result, eleven parasitoid species were established: Chorebus albipes (Haliday) (Hymenoptera, Braconidae), Pediobius metallicus (Nees), Danuviella subplana Erdos, Chrysocharis nephereus (Walker), Chrysocharis pubicornis (Zetterstedt), Diglyphus crassinervis Erdos, Diglyphus isaea (Walker), Diglyphus albiscapus Erdos, Cirrospilus diallus Walker, Cirrospilus pictus (Nees) (Hymenoptera, Eulophidae) and Elasmus sp. (Hymenoptera, Elasmidae). Six species (D. crassinervis, D. isaea, D. albiscapus, C. diallus, C. pictus and Elasmus sp.) have been recorded as new parasitoids of P. populi. The genus Danuviella was found for the first time in Bulgaria. Five species (C. albipes, P. metallicus, D. subplana, D. crassinervis and D. albiscapus) are new records for the fauna of the country. The total mortality of P. populi caused by parasitoids was 19.1?% and 49.0?% for the overwintering and the summer generations, respectively. P. metallicus was the most important in reducing the pest number.  相似文献   

Impact of moisture content on dynamic mechanical properties and transition temperatures of wood     

Oleg V. Startsev  Alexey Makhonkov  Vladimir Erofeev  Sergei Gudojnikov 《Wood material science & engineering》2017,12(1):55-62

The dynamic shear modulus and the loss modulus of Betula alba, Ulmus parvifolia, Quercus robur, Acer platanoides, Tilia cordata, Fraxinus excelsior and Pinus sylvestris wood were measured using an inverted torsion pendulum within a wide temperature range. The glass transition temperature of the lignin–carbohydrate complex and the decomposition temperature of the wood cellulose were estimated. The temperature band from 170°C to 240°С shows the transition of the lignin–cellulose complex from the glassy to the rubbery state. Mechanical properties of different types of wood are affected by moisture and anatomical differences, but glass transition and decomposition temperatures are the same. More than 5% of moisture in the wood stored at normal conditions were found. After drying, the increase of dynamic shear modulus of wood over the entire region of the glassy state was observed. The intensity of maximum peak of dynamic loss modulus is also increased due to activation of the segmental motion of macromolecules of the ligno-carbon complex. The decomposition temperature of the cellulose crystallites was unchanged for specimens containing moisture and for dried specimens.  相似文献