Fitness costs of Vip3A resistance in Spodoptera frugiperda on different hosts     

Xuan Chen  Graham P Head  Paula Price  David L Kerns  Marlin E Rice  Fangneng Huang  Ryan T Gilreath  Fei Yang 《Pest management science》2019,75(4):1074-1080

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秤锤树叶片内生真菌的分离鉴定及其对植株生长的影响     

李霞  曹昆  丛伟 《广西农业生物科学》2010,(1):75-81

本研究从秤锤树叶片中分离内生菌进行分子鉴定,并接种到无菌组培苗中研究其对植株生长的影响。研究结果显示,从秤锤树叶片中共分离获得7株真菌。扩增出的6个菌株经ITS测序,在NCBI网站上进行基因序列比对,鉴定出其中5株菌株均属于球毛壳菌（Chaetomium globosum）,1株属于子囊菌（As-comycete）。进一步实验表明：分离的菌株多数对组培苗的株高生长影响不大,可初步鉴定其为内生菌,其中的属于球毛壳菌的F和H菌株对无菌苗的株高有明显的促进作用。  相似文献   

日本晴与5个优良恢复系的多态性标记筛选及遗传差异分析   总被引：3，自引：0，他引：3       下载免费PDF全文

赵芳明  郭超  魏霞  杨正林  凌英华  桑贤春  王楠  张长伟  李云峰  何光华   《西南大学学报(自然科学版)》2016,38(11):1-7

遗传基因的分离需要供、受体亲本间较大的遗传差异.该文利用均匀分布于水稻12条染色体的429个SSR标记,分析了日本晴与5个自育优良恢复系之间的多态性标记及分布密度,进而评价它们间的遗传差异性.结果显示,日本晴与西恢18号、缙恢35、R225、R232和R250之间的多态性标记数分别为263,253,253,255和240个;多态率分别为61.31%,58.97%,58.97%,59.44%和55.94%;每个标记间的平均物理距离分别为1.59,1.61,1.62,1.62和1.72 Mb.每个SSR位点可检测到的等位基因数目为2~5个,平均每对SSR引物检测到2.35个等位基因.各亲本之间的遗传距离在0.121 7~0.896 7之间,日本晴与5个恢复系间的平均遗传距离为0.857 6.聚类分析也将日本晴与5个恢复系分为两大亚类,表明它们之间存在极大的遗传差异.这些材料和结果对水稻遗传基因的分离具有重要意义.  相似文献   

Insecticidal properties of monoterpenoid derivatives to the house fly (diptera: Muscidae) and red flour beetle (coleoptera: Tenebrionidae)     

Pamela J. Rice  Joel R. Coats 《Pest management science》1994,41(3):195-202

Monoterpenoid derivatives were synthesized and their insecticidal activities were evaluated against red flour beetles, Tribolium castaneum (Herbst), in fumigant bioassays and against house flies Musca domestica (L.) in topical, fumigant, and ovicidal bioassays. Acetate derivatives and haloacetate derivatives were compared with each other, and with the parent monoterpenoid to determine structure-activity relationships. Acetate derivatives were more active than the propionate derivatives of cyclic monoterpenoids in the topical, fumigant, and ovicidal bioassays. Pivalates were topically more insecticidal than acetates to adult house flies, while the acetates had the greater ovicidal activity. Acetates and pivalates were more effective than haloacetates in the topical, red flour beetle fumigation and ovicidal bioassays. Fluoroacetates of cyclic monoterpenoids were the most effective house fly fumigants, followed by acetates, and trichloroacetates. Several derivatives were produced that displayed enhanced activity relative to the parent alcohols or phenols.  相似文献   

Clinical pathology of myodegeneration (pancreas disease) in Atlantic salmon (Salmo salar)     

H W Ferguson  D A Rice  J K Lynas 《The Veterinary record》1986,119(12):297-299

The clinical pathology and histopathology of two groups of Atlantic salmon with severe degenerative myopathy (pancreas disease) is described and compared with a third healthy group. One affected group was anorexic and had low plasma protein and albumin levels while the other was feeding and had normal levels. Both diseased groups had plasma and tissue vitamin E and selenium levels lower than the healthy group. Similarly, creatine kinase values were raised in affected groups. If representative of the syndrome as a whole, the results suggest that the myopathy of pancreas disease has a basis in a vitamin E-selenium deficiency, but whether primary or induced is not clear. The results also demonstrate that the myopathy and pancreatic atrophy do not inevitably lead to anorexia or any other clinically obvious sign of disease, despite both cardiac and oesophageal involvement.  相似文献   

Factors affecting dystocia in beef heifers   总被引：3，自引：0，他引：3  

L E Rice  J N Wiltbank 《Journal of the American Veterinary Medical Association》1972,161(11):1348-1358

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Serological Studies of Parasitized Cattle: II. Electrophoretic Properties of Serial Serum Samples          下载免费PDF全文

Christine E. Rice  H. J. Smith    D. Cochrane 《Canadian journal of veterinary research》1967,31(2):37-42

Electrophoretic examinations using cellulose acetate strips were made on 17 serum samples collected at two-week intervals from each of three calves before and for about four months after they were placed on parasite-infected pastures in June, 1964. Four additional serum samples taken during the winter months when the animals were stabled and two obtained after they were again exposed to infected pastures in June 1965, were also examined. Two control animals of the same age, that were placed on clean pasture in 1964 and not exposed to infected pasture until June 1965, were bled on the same dates and their sera examined in parallel. All five calves showed an increase in the proportion and amount of γ-globulin in their serum as they increased in age but the gain was greatest in the two exposed calves that developed the highest complement-fixing titres with nematode antigens. Complement-fixing titres rose slowly in the other exposed calf and its serum γ-globulin level remained relatively comparable to that of the two controls.  相似文献   

Complement Titres of Naturally and Artificially Raised Piglets II. Comparison with the Results of Electrophoretic Examinations          下载免费PDF全文

Delma Cochrane  C. L'Ecuyer    Christine E. Rice 《Canadian journal of veterinary research》1964,28(2):38-42

Serial serum samples from piglets in one naturally-raised and three artificially-raised litters, 19 animals, were examined by cellulose acetate electrophoresis. The electropherograms of all day-old suckling and colostrum deprived piglets showed five definite bands, the first, third, fourth and fifth corresponding in position to the albumin, alpha-, beta- and gamma-globulin regions for adult pig serum. The second, well-defined band was located between the albumin and main alpha-globulin bands. This component, considered to be alpha₁-globulin or fetuin, was no longer visible as a distinct band in the electrophoretic pattern for sera of three-week old suckling piglets or of five-week old artificially-raised piglets. No consistent relationship was observed between the rate of increase in haemolytic complement titre and the proportional changes in the various categories of serum globulins.  相似文献   

Quantum mechanics. To condense or not to condense     

Rice TM 《Science (New York, N.Y.)》2002,298(5594):760-761

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Genetic characterization of 2 novel feline caliciviruses isolated from cats with idiopathic lower urinary tract disease     

Rice CC  Kruger JM  Venta PJ  Vilnis A  Maas KA  Dulin JA  Maes RK 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(3):293-302

Feline caliciviruses (FCVs) are potential etiologic agents in feline idiopathic lower urinary tract disease (I-LUTD). By means of a modified virus isolation method, we examined urine obtained from 28 male and female cats with nonobstructive I-LUTD, 12 male cats with obstructive I-LUTD, and 18 clinically healthy male and female cats. All cats had been routinely vaccinated for FCV. Two FCVs were isolated; I (FCV-U1) from a female cat with nonobstructive I-LUTD, and another (FCV-U2) from a male cat with obstructive I-LUTD. To determine the genetic relationship of FCV-U1 and FCV-U2 to other FCVs. capsid protein gene RNA was reverse transcribed into cDNA, amplified, and sequenced. Multiple amino acid sequence alignments and phylogenetic trees were constructed for the entire capsid protein, hypervariable region E, and the more conserved (nonhypervariable) regions A, B, D, and F. When compared to 23 other FCV isolates with known biotypes, the overall amino acid sequence identity of the capsid protein of FCV-U1 and FCV-U2 ranged from 83 to 96%; identity of hypervariable regions C and E ranged from 58 to 85%. Phylogenetically, FCV-U1 clearly separated from other FCV strains in phenograms based on nonhypervariable regions. In contrast, FCV-U2 consistently segregated with the Urbana strain in all phenograms. Clustering of isolates by geographic origin was most apparent in phenograms based on nonhypervariable regions. No clustering of isolates by biotype was apparent in any phenograms. Our results indicate that FCV-UI and FCV-U2 are genetically distinct from other known vaccine and field strains of FCV.  相似文献