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11.
Immunohistochemical Expression of Growth Factors in the Follicular Wall of Normal and Cystic Ovaries of Sows
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FJF Sant'Ana JL Reis Junior GR Blume EJ Gimeno F Rey HH Ortega 《Reproduction in domestic animals》2015,50(2):327-332
The expression of growth factors was evaluated immunohistochemically in normal and cystic ovaries of sows. The immunohistochemically stained area (IHCSA) was quantified by image analysis to analyse the expression of these proteins in the follicular wall of secondary, tertiary and cystic follicles. IGF‐I immunoreactivity was strong in the granulosa cell layer (GC), moderate in the theca interna (TI) and mild in the theca externa (TE) of the normal follicles. There was severe reduction of the labelling to IGF‐I in the GC of the follicular and luteinized cysts. In the normal follicles, the reactivity for IGF‐II was very similar to pattern noted in IGF‐I. There was reduction of the IHCSAs in the GC of the follicular and luteinized cysts, but the decrease was not significant. The staining of the IGF‐II in the TI and TE of the cysts was increased, in comparison with normal follicles. The IHCSAs for VEGF were higher in the GC and TE of the normal follicles in contrast to TI, but this difference was noted only in the tertiary follicle. The VEGF reactivity increased in the GC of the cysts, in relation to normal follicles. The results of the current study show that the formation of ovarian cysts in sows is associated with alterations in the immunohistochemical expression of some growth factors. 相似文献
12.
Ana Bartual Néstor Arandia-Gorostidi Andrés Cózar Soledad Morillo-García María Jesús Ortega Montserrat Vidal Ana María Cabello Juan Ignacio González-Gordillo Fidel Echevarría 《Marine drugs》2014,12(2):682-699
Polyunsaturated aldehydes (PUAs) are organic compounds mainly produced by diatoms, after cell wounding. These compounds are increasingly reported as teratogenic for species of grazers and deleterious for phytoplanktonic species, but there is still scarce information regarding concentration ranges and the composition of PUAs in the open ocean. In this study, we analyzed the spatial distribution and the type of aldehydes produced by the large-sized (>10 μm) phytoplankton in the Atlantic Ocean surface. Analyses were conducted on PUAs released after mechanical disruption of the phytoplankton cells, referred to here as potential PUAs (pPUAs). Results show the ubiquitous presence of pPUA in the open ocean, including upwelling areas, as well as oligotrophic gyres. Total pPUA concentrations ranged from zero to 4.18 pmol from cells in 1 L. Identified PUAs were heptadienal, octadienal and decadienal, with heptadienal being the most common (79% of total stations). PUA amount and composition across the Atlantic Ocean was mainly related to the nitrogen:phosphorus ratio, suggesting nutrient-driven mechanisms of PUA production. Extending the range of trophic conditions considered by adding data reported for productive coastal waters, we found a pattern of PUA variation in relation to trophic status. 相似文献
13.
Ortega Jaime Díaz Sedov Sergey Romero Francisco Jardines Luis Gerardo Martínez Rebolledo Elizabeth Solleiro 《Journal of Soils and Sediments》2022,22(3):942-956
Journal of Soils and Sediments - The objective of this study was to examine the pedogenetic evolution occurring in technic hard materials from an iron mine through the characterization of a... 相似文献
14.
Raquel Sánchez-Pérez Encarnación Ortega Henri Duval Pedro Martínez-Gómez Federico Dicenta 《Euphytica》2007,155(3):381-391
The inheritance of 16 important agronomic traits and its relationship were studied for four years in a population of 167 almond
[P. dulcis (Mill.) D.A. Webb] seedlings obtained from a cross between the French selection ‘R1000’ (‘Tardy Nonpareil’ × ‘Tuono’) and
the Spanish cultivar ‘Desmayo Largueta’. For some traits (blooming and leafing date) descendants segregated between the value
of both progenitors, meanwhile for others the mean of the offspring was lower (bloom density, productivity and ripening date)
or higher (in-shell/kernel ratio and double kernels). As expected, kernel bitterness and self-incompatibility behaved as monogenic
traits. Some important correlations between traits were detected. The implications of the transmission and the correlation
of these traits in the breeding programmes are discussed. 相似文献
15.
Silva RD Bueno AL Gallon CW Gomes LF Kaiser S Pavei C Ortega GG Kucharski LC Jahn MP 《Fitoterapia》2011,82(6):818-826
This study analyzed the plasma lipid profile, glucose levels and fat deposits in male rats treated with aqueous extract of gross yerba mate, commercial yerba mate or water. Yerba mate treatment did not change body weight gain and lipid profile. The consumption of gross yerba mate significantly increased blood glucose (6.6 mmol/L) as compared to the water (4.8 mmol/L) and commercial group (5.2 mmol/L) and decreased epididymal and intra-abdominal deposits (10.1 mg/g and 23.7 mg/g of weight) as compared to the water (15.4 mg/g and 36.9 mg/g of weight) and commercial group (12.5 mg/g and 28 mg/g of weight). The results suggest that gross yerba mate reduces fat more efficiently but produces a greater increase in blood glucose when compared to commercial yerba mate and water groups. 相似文献
16.
17.
O. R. Ortega E. Duran C. Arbizu R. Ortega W. Roca D. Potter C. F. Quiros 《Genetic Resources and Crop Evolution》2007,54(4):807-821
This paper analyzes the genetic diversity of mashua cultivars grown in six communities in the Cusco region of Perú, of non-cultivated
forms collected in the same region, and a sample of cultivars from the germplasm collection at the International Potato Center
(CIP). From the DNA fingerprinting generated by SRAP markers, it is clear that mashua is a genetically variable crop with
a range of similarity ranging from 65 to 99%. The widest range of variation was found for the most isolated community, Sayllafaya.
Another important finding was that most of the non-cultivated accessions are likely feral races resulting from escape to cultivation
rather than wild relatives. In general the range of variation of the cultivars from the communities and their feral relatives
are not represented in the cultivars sampled of the collection maintained at the International Potato Center, Perú. 相似文献
18.
Serra-Majem L Ribas L Ngo J Ortega RM García A Pérez-Rodrigo C Aranceta J 《Public health nutrition》2004,7(7):931-935
OBJECTIVE: To evaluate dietary habits in Spanish children and adolescents based on a Mediterranean Diet Quality Index tool, which considers certain principles sustaining and challenging traditional healthy Mediterranean dietary patterns. DESIGN: Observational population-based cross-sectional study. A 16-item Mediterranean Diet Quality Index was included in data gathered for the EnKid study (in which two 24-hour recalls, a quantitative 169-item food-frequency questionnaire and a general questionnaire about socio-economic, demographic and lifestyle items were administered). SETTING: Spain. SUBJECTS: In total, 3850 children and youths aged 2-24 years residing in Spain. RESULTS: Of the sample, 4.2% showed very low KIDMED index results, 49.4% had intermediate values and 46.4% had high index results. Important geographical differences were seen, with subjects from the Northeast showing the most favourable outcomes (52% with elevated scores vs. 37.5% of those from the North). Lower percentages of high diet quality were observed in low socio-economic groups, compared with middle and upper income cohorts (42.8%, 47.6% and 54.9%, respectively). Large cities had more positive results and only slight variations were seen for gender and age. CONCLUSIONS: The KIDMED index, the first to evaluate the adequacy of Mediterranean dietary patterns in children and youth, confirms that this collective is undergoing important changes, which makes them a priority target for nutrition interventions. Results challenge certain commonly perceived notions tied to income level, population size and diet quality. 相似文献
19.
Phosphorus forms and content were studied in soils of the Lomas de Arequipa (Atacama desert, Peru) using a fractionation method. These Lomas are small hills periodically submitted to the El Niño-Southern Oscillation (ENSO) which causes heavy rainfall. Sample soils were randomly selected in five landscape types characterized by vegetation: cactaceae (Cac), cactaceae and herbaceous (CacHerb), shrubs (Shr), trees with cover < 60% (Tree) and shrubs or trees with cover > 60%) (ShrTree). All the soils were strongly acidic and classified as loamy sand, sandy loam or silt loam. Organic carbon content was under 1% in Cac or CacHerb, then increased strongly in ShrTree (6.50%). Considering phosphorus, all the forms (labile as well resistant forms) increased markedly from Cac soils to ShrTree soils. In all the soils, the labile forms (Resin-P: range 45–105 μg g− 1; NaHCO3-Pi: 23–123 μg g− 1; or NaHCO3-Po: 10–122 μg g− 1) were very high. These high phosphorus contents were attributed to the specific climatic conditions of the Lomas that feature a long period of vegetation dormancy (very dry period) and a short period of growth, following ENSO-associated precipitation. We suggested that during the dry period, plant decay and microbial cells death lead to release and accumulation of labile P in the soil, the rainfall wetting the soil, permitting vegetation growth. In this respect, the Lomas climatic conditions contribute to soil fertility, especially as labile forms of phosphorus are chiefly concerned. 相似文献
20.
Albillos SM Busto MD Perez-Mateos M Ortega N 《Journal of agricultural and food chemistry》2005,53(15):6094-6099
The effect of the ripening time on the proteolytic process in cheeses manufactured from mixtures of cow's and ewe's milk during a 167-day ripening period was monitored by capillary electrophoresis of the pH 4.6-insoluble fraction. Totals of 21 and 16 peaks were recognized and matched in the electropherograms obtained with a fused-silica capillary and a neutral capillary (hydrophilically coated), respectively. These peaks corresponded to intact bovine and ovine caseins and their hydrolysis products (e.g., alpha(s1)-casein, gamma-caseins). In 167-day-old cheeses, bovine alpha(s0)-casein (alpha(s1)-casein 9P) had been completely degraded and 6% of the residual bovine alpha(s1)-casein remained intact. Breakdown of the beta-casein fraction was lower than that of the alpha(s)-casein fraction. Finally, partial least-squares regression and principal component regression were used to predict the ripening time in cheeses. The root-mean-square errors in prediction by cross-validation were <7.8 days in all cases. 相似文献