Disease caused by Yersinia ruckeri serotype O2b found in Chilean‐farmed coho salmon,Oncorhynchus kisutch (Walbaum, 1792)          下载免费PDF全文

R Avendaño‐Herrera  D Tapia‐Cammas  A Aedo  P Saldivia  C Ortega  R Irgang 《Journal of fish diseases》2017,40(2):279-285

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Effect of dietary protein content on growth rate,survival and body composition of juvenile cauque river prawn,Macrobrachium americanum (Bate 1868)          下载免费PDF全文

Yuniel Méndez‐Martínez  Stig Yamasaki‐Granados  Marcelo U García‐Guerrero  Luis R Martínez‐Córdova  Marta E Rivas‐Vega  Fabiola G Arcos‐Ortega  Edilmar Cortés‐Jacinto 《Aquaculture Research》2017,48(3):741-751

We determined the effect of four concentrations of dietary crude protein, 30.7, 37.2, 41.8 and 46.8% on growth rate, survival and body composition of the juvenile cauque river prawn (Macrobrachium americanum). The prawns were hatched in the laboratory from the spawn of one wild ovigerous female. Prawns consuming 37.2% crude protein reached a final weight of 0.58 g (feed conversion ratio of 2.15), which was significantly better than the other treatments. Survival was 100% in all treatments. Protein content in the diets had no significant effect on whole body proximate composition and amino acid profile. Juveniles consuming the 37.2% crude protein diet grew faster than those fed the other diets. Specific growth rate was adjusted to the two‐slope broken‐line regression analysis model to estimate the optimal protein requirement. In conclusion, these results demonstrated that the 37.2% protein level diet is optimal for juvenile cauque river prawn M. americanum in the experimental conditions of this study.  相似文献   

Energy analysis of irrigation delivery systems: monitoring and evaluation of proposed measures for improving energy efficiency   总被引：2，自引：1，他引：1  

M. A. Moreno  J. F. Ortega  J. I. Córcoles  A. Martínez  J. M. Tarjuelo 《Irrigation Science》2010,28(5):445-460

In Spain, the Ministry of Industry is implementing actions for analyzing the energy efficiency of Water User Associations (WUAs) by using energy indicators and proposing measures to improve the use of energy. The main objective of this work was to develop tools to improve energy efficiency in WUAs. These tools were validated by utilizing them in the energy analysis of 15 WUAs located in Castilla-La Mancha Region (Spain) during the 2007 irrigation season. These tools were also utilized for the proposal of measures to improve the use of energy. The proposed measures were monitored and evaluated in 7 of the 15 WUAs during the 2008 irrigation season. The developed tools were integrated into a Decision Support System for performing energy analysis and for proposing measures of energy efficiency improvement. In most of the study cases, an improvement of the energy efficiency after the implementation of the proposed measures was detected, with an average energy saving of the 10.2%.  相似文献   

Spatial segregation of striped marlin (Kajikia audax) by size in the eastern Pacific Ocean     

Tatiana A. Acosta‐Pachn  Mariana L. Muzquiz‐Villalobos  Sofia Ortega‐García  Raúl O. Martínez‐Rincn 《Fisheries Oceanography》2019,28(2):203-211

Striped marlin (Kajikia audax) is an epipelagic fish distributed in oceanic and coastal waters of the Pacific Ocean. This species is usually found in warm and coastal waters with high primary productivity. The main goal of this study was to describe the spatial segregation of striped marlin by average Eye‐Fork length (EFL) in the eastern Pacific Ocean (EPO) and its relationship with environmental variables using EFL data obtained from tuna purse‐seining and Generalized Additive Models (GAMs). The model suggested that larger individuals of striped marlin were more likely to be found in waters with high Chlorophyll‐a concentration (>2 mg/m³) and with temperatures lower than 25°C, within a region known as the “cold tongue” and the Humboldt current system, while smaller individuals were more likely to be found in warmer and low productive areas within a region known as the “warm pool of the EPO.” We observed that set type caused a large variation on average EFL of striped marlin; larger fish were captured in sets associated with floating objects (natural and manmade), while smaller fish were captured in sets associated with dolphins. Despite this, our findings suggest that striped marlin has a latitudinal gradient in average EFL; larger individuals occurred predominantly south of 10°N, while smaller ones occurred predominantly in coastal waters between 10°N and 20°N, thus demonstrating a spatial segregation of the species affected by its maturity stage.  相似文献   

Induced resistance: a tool for fungicide resistance management     

Felipe Ortega  Ulrike Steiner  Heinz W. Dehne 《Pest management science》1998,53(2):193-196

Induced resistance to the apple scab fungus Venturia inaequalis was demonstrated in greenhouse tests with 12-day-old seedlings of the apple cultivar Golden Delicious treated with methyl 2,6-dichloro-isonicotinate or 3,5-dichlorosalicylic acid prior to inoculation with the causal fungus. Studies of the dose-response of flusilazole on induced resistant plants revealed synergistic effects between both crop protection principles. Therefore, the use of such resistance-inducing compounds in the field might allow a reduction in the number of fungicide applications, and possibly a reduction in dose, thus resulting in improved efficacy of fungicides. There was also evidence that induced resistance could prove to be a valid strategy for the treatment of pathogen populations with reduced sensitivity to a given fungicide. © 1998 SCI  相似文献   

Saline Year-Gradients in the Lagoon “La Salina,” Tonameca,Oaxaca, Mexico: Effects on Growth of Mangrove Biotope     

Ygor Ortega Mikolaev  Edgar Sánchez Bernal  José Bojorges Baños  Manuel Ortega Escobar  José García Hernández 《Water, air, and soil pollution》2016,227(11):426

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Effects of starvation on δ15N and δ13C in Atlantic bonito,Sarda sarda (Bloch, 1793)          下载免费PDF全文

José L Varela  Aurelio Ortega  Fernando de la Gándara  Antonio Medina 《Aquaculture Research》2015,46(8):2043-2047

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Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian Cysts     

NR Salvetti  C Baravalle  GA Mira  EJ Gimeno  BE Dallard  F Rey  HH Ortega 《Reproduction in domestic animals》2009,44(5):805-814

The purpose of this study was to estimate the expression and relative amounts of estrogen (ER) and progesterone receptors (PR) and their isoforms as well as heat shock protein 70 (HSP70) in ovaries of rats with induced cystic ovarian disease (COD). Primary, secondary, tertiary, atretic and cystic follicles were evaluated by immunohistochemistry and total ovarian proteins were analyzed by Western blot. In the granulosa layer, growing and cystic follicles in the treated group have a higher expression of ERα than growing follicles of control individuals. In the theca interna layer, tertiary follicles presented a significantly higher expression of ERα in the treated group. An increase in total ERα protein was detected in the treated group. Granulosa cells of all growing, atretic and cystic follicles show a lower expression of ERβ in animals with COD, and the total protein expression of ERβ was lower in this group. The expression of PR was lower in the granulosa cell layer of tertiary and cystic follicles in treated animals, and theca interna layer had less intense immunostaining in this group. Although there were no differences in the expression of PR-B by Western blotting, the expression of PR-A was higher and the expression of PR-C was smaller in the treated group. An intense HSP70 immunostaining was observed in the cells of cystic follicles. By Western blotting, higher protein expression of HSP70 was detected in the ovarian samples of the control group than those of the treated ones. Ovaries of animals with COD exhibited an altered steroid receptor expression and subtype balance as compared with control animals, and an increase in HSP70 immunoexpression.  相似文献   

Neospora caninum antibodies in commercial fetal bovine serum     

Torres MP  Ortega YR 《Veterinary parasitology》2006,140(3-4):352-355

The protozoan parasite Neospora caninum is a major cause of abortion in cattle throughout the world. In the process of propagating Neospora in vitro and producing specific antibodies for development of diagnostic assays in the food supply, our laboratory identified the presence of bovine antibodies to N. caninum in fetal bovine sera. The sera were produced commercially and preferentially recommended for tissue culture use and monoclonal antibody production. Seventeen different fetal bovine serum samples of different grades and from four different companies were examined for the presence of total IgG, IgG₁, IgG₂, and IgM specific for N. caninum. All of the tested serum samples recognized N. caninum specific bands on Western blot. Low IgG serum also recognized these antigens but with lower intensity. Antibody response was also evaluated using a commercially available ELISA kit for N. caninum.  相似文献   

A spatiotemporal study of bacterial community profiles associated with Atlantic bluefin tuna larvae,Thunnus thynnus L., in three Mediterranean hatcheries     

François‐Joël Gatesoupe  Denis Covès  Aurelio Ortega  Nikos Papandroulakis  Olav Vadstein  Fernando de la Gándara 《Aquaculture Research》2013,44(10):1511-1523

During the first 2 years of larval rearing trials with Atlantic bluefin tuna, survival was a challenging issue. As bacterial colonization of the gut has been shown to play a key role for other species, we studied the profiles of the microbiota associated with individual larvae at different stages in three distant hatcheries. The Bacterial Community Profile (BCP) was quantified based on PCR‐DGGE analyses of partial amplicons from 16S rDNA. Considerable individual variability in BCP was observed before onset of feeding, and the BCP did not show regular fluctuation during ontogenesis. Microalgae were added to the rearing tanks in two of the three hatcheries, but it was not possible to distinguish the effect of location from the effect of algal addition on BCP. In one hatchery, the larvae were reared either with algal addition or in mesocosm, but due to high individual variability, no significant difference in BCP was detected between the two groups. It was hypothesized that this variability was caused by differences in health, physiological status and developmental stage of the larvae. A practical conclusion from the study is the need to analyse a considerable number of individuals to reflect statistically significant differences between the microbial communities associated with rearing groups.  相似文献