New neurons follow the flow of cerebrospinal fluid in the adult brain   总被引：1，自引：0，他引：1  

Sawamoto K  Wichterle H  Gonzalez-Perez O  Cholfin JA  Yamada M  Spassky N  Murcia NS  Garcia-Verdugo JM  Marin O  Rubenstein JL  Tessier-Lavigne M  Okano H  Alvarez-Buylla A 《Science (New York, N.Y.)》2006,311(5761):629-632

In the adult brain, neuroblasts born in the subventricular zone migrate from the walls of the lateral ventricles to the olfactory bulb. How do these cells orient over such a long distance and through complex territories? Here we show that neuroblast migration parallels cerebrospinal fluid (CSF) flow. Beating of ependymal cilia is required for normal CSF flow, concentration gradient formation of CSF guidance molecules, and directional migration of neuroblasts. Results suggest that polarized epithelial cells contribute important vectorial information for guidance of young, migrating neurons.  相似文献   

Evaluation of modified vaccinia virus Ankara expressing VP2 protein of infectious bursal disease virus as an immunogen in chickens     

Flavia Adriana Zanetti  Mar��a Paula Del M��dico Zajac  Oscar Alberto Taboga  Gabriela Calamante 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(2):199-201

A recombinant modified vaccinia Ankara (MVA) virus expressing mature viral protein 2 (VP2) of the infectious bursal disease virus (IBDV) was constructed to develop MVA-based vaccines for poultry. We demonstrated that this recombinant virus was able to induce a specific immune response by observing the production of anti-IBDV-seroneutralizing antibodies in specific pathogen-free chickens. Besides, as the epitopes of VP2 responsible to induce IBDV-neutralizing antibodies are discontinuous, our results suggest that VP2 protein expressed from MVA-VP2 maintained the correct conformational structure. To our knowledge, this is the first report on the usefulness of MVA-based vectors for developing recombinant vaccines for poultry.  相似文献   

Seroprevalence of Toxoplasma gondii and Neospora caninum in feral cats (Felis silvestris catus) in Majorca, Balearic Islands, Spain     

Javier Milln  Oscar Cabezn  Marcela Pabn  J.P. Dubey  Sonia Almería 《Veterinary parasitology》2009,165(3-4):323-326

Cryptosporidium spp. are common intestinal protozoan parasites that infect a wide range of hosts, including humans and livestock, worldwide. The objective of this study was to determine the prevalence of Cryptosporidium spp. in dairy calves in Prince Edward Island, Canada, and the potential for transmission of this parasite between dairy calves and humans. Fecal samples were collected from 183 dairy calves from 11 farms in Prince Edward Island. The prevalence of Cryptosporidium spp. infections in these animals was determined by examining for the presence of oocysts in the fecal samples, using immunofluorescence microscopy. Molecular characterization was done using a nested-PCR protocol to amplify fragments of the Cryptosporidium heat-shock protein 70 gene, followed by DNA sequencing. Ten calves (6.2%), representing 4 out of 11 farms tested, were positive for Cryptosporidium spp. DNA sequence analysis on five PCR positive samples demonstrated that Cryptosporidium parvum was the only species present in the calves tested, suggesting that there is a potential risk of zoonotic transmission between dairy calves and humans in this region.  相似文献   

The prevalence of Toxocara cati in domestic cats in Mexico City   总被引：2，自引：0，他引：2  

Martínez-Barbabosa I  Vázquez Tsuji O  Cabello RR  Cárdenas EM  Chasin OA 《Veterinary parasitology》2003,114(1):43-49

The faeces of 520 domestic cats, resident in the 16 municipal authorities of Mexico City, were analysed using the method of centrifuging with zinc sulphate (at 33%). Three hundred and ninety-nine animals lived in houses, 121 in apartments. For the purpose of this study they were divided into seven age groups, of 6 months each. Toxocara cati eggs were found in the faeces of 42.5% of the animals. 20.7% of apartment cats and 49.1% of house cats were infected. This difference in the rate of infection is statistically significant (P<0.002.) for the house cats. T. cati was found in all the age groups although most infection was found in animals less than 1 year old.The fact that infection occurred in the whole age range of the cats and in all the municipal authorities means that the risk of being contaminated with this helminth in Mexico City is high. As a consequence, the possibility of developing illness as a result of becoming a host to larva migrans is also high.  相似文献   

RADIOGRAPHIC DIAGNOSIS-TRACHEAL EOSINOPHILIC GRANULOMA/POLYP     

Oscar  Ramirez  III  DVM  Eleanor C.  Hawkins  DVM 《Veterinary radiology & ultrasound》1998,39(5):422-424

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Detection of Leishmania infantum in captive wolves from Southwestern Europe     

Sastre N  Francino O  Ramírez O  Enseñat C  Sánchez A  Altet L 《Veterinary parasitology》2008,158(1-2):117-120

The aim of the present study was to determine the prevalence of Leishmania infantum infection in a wild reservoir host (Canis lupus) throughout an endemic area for the disease (Southern Europe). For that reason, the serum and peripheral blood samples of 33 captive wolves from the European Breeding of Endangered Species Programme (EEP) were analyzed using the enzyme-linked immunosorbent assay (ELISA) and real-time quantitative PCR (qPCR). L. infantum was detected in three samples from Central Portugal and Central and Northern Spain. Even though L. infantum infection in positive samples was low, surveillance of zoonotic leishmaniosis in this population is recommended as the parasite load could be higher in other tissues due to parasite tropism and most of the EEP institutions studied are located in endemic areas for canine leishmaniosis in Europe.  相似文献   

RADIOGRAPHIC DIAGNOSIS: EFFECT OF RIGHT VS. LEFT LATERAL RECUMBENCY ON MYELOGRAPHIC APPEARANCE OF A LATERALIZED EXTRADURAL MASS     

Mark L.  Matteucci  DVM  Oscar  Ramirez III  DVM  Donald E.  Thrall  DVM  PhD 《Veterinary radiology & ultrasound》1999,40(4):351-352

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Salmon cells SHK‐1 internalize infectious pancreatic necrosis virus by macropinocytosis     

Jorge Levicn‐Asenjo  Ricardo Soto‐Rifo  Francisco Aguayo  Aldo Gaggero  Oscar Leon 《Journal of fish diseases》2019,42(7):1035-1046

We have previously shown that infectious pancreatic necrosis virus (IPNV) enters the embryo cell line CHSE‐214 by macropinocytosis. In this study, we have extended our investigation into SHK‐1 cells, a macrophage‐like cell line derived from the head kidney of Atlantic salmon, the most economically important host of IPNV. We show that IPNV infection stimulated fluid uptake in SHK‐1 cells above the constitutive macropinocytosis level. In addition, upon infection of SHK‐1 cells, IPNV produced several changes in actin dynamics, such as protrusions and ruffles, which are important features of macropinocytosis. We also observed that the Na+/H+ pump inhibitor EIPA blocked IPNV infection. On the other hand, IPNV entry was independent of clathrin, a possibility that could not be ruled out in CHSE 214 cells. In order to determine the possible role of accessory factors on the macropinocytic process, we tested several inhibitors that affect components of transduction pathways. While pharmacological intervention of PKI3, PAK‐1 and Rac1 did not affect IPNV infection, inhibition of Ras and Rho GTPases as well as Cdc42 resulted in a partial decrease in IPNV infection. Further studies will be required to determine the signalling pathway involved in the macropinocytosis‐mediated entry of IPNV into its target cells.  相似文献   

Fine‐scale population structure and connectivity of bottlenose dolphins,Tursiops truncatus,in European waters and implications for conservation     

Milaja Nyknen  Marie Louis  Eileen Dillane  Eric Alfonsi  Simon Berrow  Joanne O'Brien  Andrew Brownlow  Pablo Covelo  Willy Dabin  Rob Deaville  Renaud de Stephanis  Franois Gally  Pauline Gauffier  Simon N. Ingram  Tamara Lucas  Luca Mirimin  Rod Penrose  Emer Rogan  Mnica A. Silva  Benoit Simon‐Bouhet  Oscar E. Gaggiotti 《水产资源保护:海洋与淡水生态系统》2019,29(Z1):197-211

ULTRASONOGRAPHIC DIAGNOSIS CYSTIC PROSTATIC HYPERPLASIA     

Oscar  Ramirez III  DVM  Linda D.  Homco  DVM 《Veterinary radiology & ultrasound》1995,36(2):146-147

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