Highly pathogenic avian influenza virus infection in chickens but not ducks is associated with elevated host immune and pro-inflammatory responses     

Suresh V Kuchipudi  Meenu Tellabati  Sujith Sebastian  Brandon Z Londt  Christine Jansen  Lonneke Vervelde  Sharon M Brookes  Ian H Brown  Stephen P Dunham  Kin-Chow Chang 《Veterinary research》2014,45(1)

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Number,causes and destinations of horses leaving the Australian Thoroughbred and Standardbred racing industries     

PC Thomson  AR Hayek  B Jones  DL Evans  PD McGreevy 《Australian veterinary journal》2014,92(8):303-311

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An evaluation of a microbial inoculum in promoting organic C decomposition in a paddy soil following straw incorporation     

Peng Su  Philip C. Brookes  Yan He  Jianjun Wu  Jianming Xu 《Journal of Soils and Sediments》2016,16(6):1776-1786

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In situ Evidence for the Association of Total Coliforms and Escherichia coli with Suspended Inorganic Particles in an Australian Reservoir     

Matthew R. Hipsey  Justin D. Brookes  Rudi H. Regel  Jason P. Antenucci  Michael D. Burch 《Water, air, and soil pollution》2006,170(1-4):191-209

The results of a field experiment that investigated the in situ association of total coliforms (TC) and E. coli (EC) with suspended inorganic particles in a drinking water reservoir are presented. The experimental program measured TC and EC at various locations and at multiple depths in a medium sized Australian reservoir subject to continuous inflow forcing. Particle concentrations and size distributions were measured using a LISST (Laser In Situ Scattering Transmissometer) profiler. Correlations between the particle measurements and the bacteria were calculated to provide in situ evidence for the association of TC and EC with suspended inorganic particles. Both TC and EC correlated most strongly with fine particles between 3.2 and 4.5 μm. Development of a simple Lagrangian model of the inflow for particles and microbes additionally provided insight into the relative roles of dilution, sedimentation, and inactivation for the bacteria. The model results and the correlations support the theory that high association with the small particles was due to their high number concentration and the effective surface area available for attachment. It was also evident that the majority of bacteria (> 80%) were physically associated with the suspended particles and allowed a quantitative estimate of the net sedimentation rate. Bacterial attachment and the concomitant increase in settling should be considered when modeling bacterial dynamics, during design of monitoring programs and when implementing pathogen risk management strategies.  相似文献   

Chloroform fumigation and the release of soil nitrogen: A rapid direct extraction method to measure microbial biomass nitrogen in soil     

P.C. Brookes  Andrea Landman  G. Pruden  D.S. Jenkinson 《Soil biology & biochemistry》1985,17(6):837-842

A new “direct extraction” method for measuring soil microbial biomass nitrogen (biomass N) is described. The new method (fumigation-extraction) is based on CHC1₃ fumigation, followed by immediate extraction with 0.5 M K₂SO₄ and measurement of total N released by CHC1₃ in the soil extracts. The amounts of NH₄-N and total N extracted by K₂SO₄ immediately after fumigation increased with fumigation time up to 5 days. Total N released by CHC1₃ after 1 day fumigation (1 day CHC1₃-N) and after 5 days fumigation (5 day CHC1₃-N) were positively correlated with the flush of mineral N (F_N) in 37 soils that had been fumigated, the fumigant removed and the soils incubated for 10 days (fumigation-incubation). The regression equations were 1 day CHC1₃-N = (0.79 ± 0.022) F_N and 5 day CHC1₃-N = (1.01 ± 0.027) F_N, both regressions accounting for 92% of the variance in the data.In field soils previously treated with ¹⁵N-labelled fertilizer, the amounts of labelled N, measured after fumigation-extraction, were very similar to the amounts of labelled N mineralized during fumigation-incubation; both were about 4 times as heavily labelled as the soil N as a whole. These results suggest that fumigation-extraction and fumigation-incubation both measure the same fraction of the soil organic N (probably the cytoplasmic component of the soil microbial biomass) and that measurement of the total N released by CHC1₃ fumigation for 24 h provides a rapid method for measuring biomass N.  相似文献   

Reply to Young et al. (2006) on the paper of Zhao et al. (2005) “Does ergosterol concentration provide a reliable estimate of soil fungal biomass?”     

X.R. Zhao  P.C. Brookes 《Soil biology & biochemistry》2006,38(6):1502-1503

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Diameter-Selective Raman Scattering from Vibrational Modes in Carbon Nanotubes   总被引：1，自引：0，他引：1  

AM Rao  E Richter  S Bandow  B Chase  PC Eklund  KA Williams  S Fang  KR Subbaswamy  M Menon  A Thess  RE Smalley  G Dresselhaus  MS Dresselhaus 《Science (New York, N.Y.)》1997,275(5297):187-191

Single wall carbon nanotubes (SWNTs) that are found as close-packed arrays in crystalline ropes have been studied by using Raman scattering techniques with laser excitation wavelengths in the range from 514.5 to 1320 nanometers. Numerous Raman peaks were observed and identified with vibrational modes of armchair symmetry (n, n) SWNTs. The Raman spectra are in good agreement with lattice dynamics calculations based on C-C force constants used to fit the two-dimensional, experimental phonon dispersion of a single graphene sheet. Calculated intensities from a nonresonant, bond polarizability model optimized for sp2 carbon are also in qualitative agreement with the Raman data, although a resonant Raman scattering process is also taking place. This resonance results from the one-dimensional quantum confinement of the electrons in the nanotube.  相似文献   

Ecology. Resilience to blooms     

Brookes JD  Carey CC 《Science (New York, N.Y.)》2011,334(6052):46-47

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Relationships between soil pH and microbial properties in a UK arable soil   总被引：1，自引：0，他引：1  

J.C. Aciego Pietri  P.C. Brookes 《Soil biology & biochemistry》2008,40(7):1856-1861

Effects of changing pH along a natural continuous gradient of a UK silty-loam soil were investigated. The site was a 200 m soil transect of the Hoosfield acid strip (Rothamsted Research, UK) which has grown continuous barley for more than 100 years. This experiment provides a remarkably uniform soil pH gradient, ranging from about pH 8.3 to 3.7. Soil total and organic C and the ratio: (soil organic C)/(soil total N) decreased due to decreasing plant C inputs as the soil pH declined. As expected, the CaCO₃ concentration was greatest at very high pH values (pH > 7.5). In contrast, extractable Al concentrations increased linearly (R² = 0.94, p < 0.001) from below about pH 5.4, while extractable Mn concentrations were largest at pH 4.4 and decreased at lower pHs. Biomass C and biomass ninhydrin-N were greatest above pH 7. There were statistically significant relationships between soil pH and biomass C (R² = 0.80, p < 0.001), biomass ninhydrin-N (R² = 0.90, p < 0.001), organic C (R² = 0.83, p < 0.001) and total N (R² = 0.83, p < 0.001), confirming the importance of soil organic matter and pH in stimulating microbial biomass growth. Soil CO₂ evolution increased as pH increased (R² = 0.97, p < 0.001). In contrast, the respiratory quotient (qCO₂) had the greatest values at either end of the pH range. This is almost certainly a response to stress caused by the low p. At the highest pH, both abiotic (from CaCO₃) and biotic Co₂ will be involved so the effects of high pH on biomass activity are confounded. Microbial biomass and microbial activity tended to stabilise at pH values between about 5 and 7 because the differences in organic C, total N and Al concentrations within this pH range were small. This work has established clear relationships between microbial biomass and microbial activity over an extremely wide soil pH range and within a single soil type. In contrast, most other studies have used soils of both different pH and soil type to make similar comparisons. In the latter case, the effects of soil pH on microbial properties are confounded with effects of different soil types, vegetation cover and local climatic conditions.  相似文献   

Microbial response to the addition of glucose in low-fertility soils   总被引：1，自引：0，他引：1  

F. C. Hoyle  D. V. Murphy  P. C. Brookes 《Biology and Fertility of Soils》2008,44(4):571-579

Addition of soluble organic substrates to soil has been shown to either increase or restrict the rate of microbial CO₂–C evolution. This has been attributed to a priming effect resulting from accelerated or decreased turnover of the soil organic matter including the soil microflora. We investigated microbial responses to small glucose-C additions (10–50 μg C g¹ soil) in arable soils either amended or not with cellulose. An immediate CO₂–C release between 0 and 69 h (equivalent to 59% of glucose-C applied) was measured. However, only half of the CO₂–C respired could be attributed to the utilisation of glucose-C substrate, based on the percentage of ¹⁴C–CO₂ evolved after the addition of a ¹⁴C-labelled glucose tracer. Thus, although no evidence of an immediate release of ‘extra’ C above the rate applied as glucose-C was observed, the pattern of decomposition for ¹⁴C-glucose suggested utilisation of an alternate C source. Based on this, a positive priming effect (1.5 to 4.3 times the amount CO₂–C evolved that was attributed to glucose-C decomposition) was observed for at least 170 h in non-cellulose-amended soil and 612 h in cellulose-amended soil. Two further phases of microbial activity in cellulose-amended soils were attributed to either activation of different microbial populations or end-product inhibition of cellulase activity after glucose addition. During these subsequent phases, a negative priming effect of between 0.1 and 1.5 times was observed. Findings indicate that the response of the microbial community to small additions of soluble organic C substrate is not consistent and support the premise that microbial response varies in a yet to be predicted manner between soil type and ecosystems. We hypothesise that this is due to differences in the microbial community structure activated by the addition of organic C and the timing of soluble organic substrate addition with respect to the current dissolved organic C status of the soil.  相似文献