Cell blocks in veterinary medicine: A comparison of two methods (cell tube and agar) in 52 effusions from dogs and cats     

Maverick Melega  Marta Santos  Mario Caniatti  Paola Valenti  Barbara Miniscalco  Majlind Sulce  Ricardo Marcos  Fulvio Riondato 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2020,49(4):632-639

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Paraoxonase-1 activity evaluation as a diagnostic and prognostic marker in horses and foals     

Beatrice Ruggerone  Saverio Paltrinieri  Alessia Giordano  Donatella Scavone  Irene Nocera  Riccardo Rinnovati  Alessandro Spadari  Licia Scacco  Paola Pratelli  Micaela Sgorbini 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(2):949-954

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Punctodera achalensis n. sp. (Nematoda: Punctoderidae), a new cyst nematode from Argentina     

Lax  Paola  Rondan Dueñas  Juan C.  Andrade  Alberto J.  Doucet  Marcelo E. 《European journal of plant pathology / European Foundation for Plant Pathology》2021,160(2):397-409

European Journal of Plant Pathology - Trichoderma aggressivum, a mycopathogen causing green mould disease, is a major problem in Agaricus bisporus cultivation due to crop loss, and resistance to...  相似文献   

Drought as a modulator of plant–virus–vector interactions: Effects on symptom expression,plant immunity and vector behaviour     

Diego F. Vasquez  Anngie Hernandez  Diana Torres  Felipe Borrero-Echeverry  Paola Zuluaga  Diego F. Rincon 《Plant pathology》2022,71(6):1282-1292

Plant–virus interactions are affected by environmental conditions that determine plant vulnerability to pathogens and the population dynamics of insect vectors. We hypothesize that drought enhances horizontal transmission by dampening the basal immunity of plants, which triggers symptom expression and vector manipulation. The potato yellow vein virus (PYVV) causes potato yellow vein disease (PYVD), a re-emerging epidemic of potato crops in South America, and is transmitted horizontally by the greenhouse whitefly (GWF), Trialeurodes vaporariorum (Hemiptera: Aleyrodidae), or vertically through infected seed tubers. We investigated the role of drought and temperature as modulators of PYVD symptom expression, plant immune response, and vector survival, development and host preference. We found that drought induced symptom expression, suppressed the salicylic acid pathway and increased PYVV replication. GWF survival was reduced on PYVV-infected hosts and development was slowest when they fed on plants with PYVD symptoms, which also triggered adults’ attraction to PYVV-infected plants. However, adults previously fed on infected plants showed the opposite effect, being more attracted to PYVV-free plants. We propose a theoretical model that explains the role of drought in modulating potato–PYVV–GWF interactions and provides new insights into plant–virus–vector coevolution.  相似文献   

Chemical composition and antimicrobial activity of Momordica charantia seed essential oil   总被引：1，自引：0，他引：1  

Braca A  Siciliano T  D'Arrigo M  Germanò MP 《Fitoterapia》2008,79(2):123-125

The essential oil obtained from the seeds of Momordica charantia was analyzed by GC/MS. Twenty-five components, representing 90.9% of the oil, were identified. The main constituents were trans-nerolidol, apiole, cis-dihydrocarveol and germacrene D. Furthermore, the oil was tested for its antibacterial and antifungal activities. Staphylococcus aureus was found to be the most sensitive microorganism with MIC values <500 microg/ml.  相似文献   

SAGE transcript profiling of the juvenile cambial region of Eucalyptus grandis     

Gallo de Carvalho MC  Caldas DG  Carneiro RT  Moon DH  Salvatierra GR  Franceschini LM  de Andrade A  Celedon PA  Oda S  Labate CA 《Tree physiology》2008,28(6):905-919

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Sequence characterized amplified region (SCAR) analysis on DNA from the three medicinal Echinacea species     

Adinolfi B  Chicca A  Martinotti E  Breschi MC  Nieri P 《Fitoterapia》2007,78(1):43-45

In our previous study, RAPD (Random Amplified Polymorphic DNA) analysis revealed species-specific markers for three medicinal Echinacea species (Asteraceae): E. angustifolia DC., E. pallida (Nutt.) Nutt. and E. purpurea (L.) Moench. In the present work, we have converted a RAPD marker (750 bp) for E. purpurea into a SCAR (Sequence Characterized Amplified Region) marker. SCAR-PCR, in fact, revealed the expected amplicon (330 bp) only in E. purpurea and not in the other two species, giving further evidence for differences in medicinal Echinacea spp. genome and confirming a greater similarity between E. pallida and angustifolia.  相似文献   

Initial cooling time before freezing affects post‐thaw quality and reproductive performance of rabbit semen          下载免费PDF全文

Michele Di Iorio  Maria Antonietta Colonna  Marsia Miranda  Paola Principe  Michele Schiavitto  Silvia Cerolini  Angelo Manchisi  Nicolaia Iaffaldano 《Animal Science Journal》2018,89(9):1240-1244

The aim of this study was to investigate the effect of initial cooling time at 5°C during semen cryopreservation on post‐thaw quality and reproductive performance of rabbit semen. Pooled semen samples (n = 6) were divided into two subsamples and cooled at 5°C for 45 or 90 min. After cooling, the semen samples were diluted to a ratio of 1:1 (v:v) with a freezing extender composed of Tris‐citrate‐glucose (TCG) containing 16% of dimethylsulfoxide and 0.1 mol/L sucrose. The semen was subsequently loaded in 0.25 ml straws, equilibrated at 5°C and frozen in liquid nitrogen vapor. After thawing, sperm motility, viability, osmotic resistance, acrosome and DNA integrity were assessed. Our results indicate that the longer cooling time, that is, 90 min before cryopreservation significantly improves sperm post‐thaw viability, motility and fertility. In fact, reproductive performances obtained with semen frozen after a 90 min cooling time were similar to those produced by fresh semen insemination. Hence, the present research provides an effective freezing protocol for rabbit semen that will allow for the creation of a sperm cryobank for the conservation of Italian rabbit genetic resources, as well as the use of frozen semen doses in commercial farms.  相似文献   

Pulmonary carcinosarcoma in a cat.     

Gabriele Ghisleni  Valeria Grieco  Marcello Mazzotti  Mario Caniatti  Paola Roccabianca  Eugenio Scanziani 《Journal of veterinary diagnostic investigation》2003,15(2):170-173

A Domestic Shorthaired cat was presented with coughing and severe respiratory distress. Thoracic radiographs revealed a lobar mass and numerous additional cavitated intrapulmonary masses. The cat was euthanized and submitted for necropsy. Histological examination of the large mass revealed 2 distinct neoplastic components consisting of bronchial adenocarcinoma admixed with neoplastic areas composed of highly atypical undifferentiated spindle cells (sarcomatous component). Simultaneous expression of vimentin and cytokeratin by a subpopulation of neoplastic epithelial cells and by rare neoplastic spindle cells was identified. On the basis of histology and immunohistochemical results, a diagnosis of primary pulmonary carcinosarcoma with intrapulmonary epithelial metastases was made. Pulmonary carcinosarcoma is a well-known pathological entity in humans. It is a rare tumor in animals and has not been previously reported in cat.  相似文献   

Assessment of leaching potential of aldicarb and its metabolites using laboratory studies     

Luca Fava  Paola Bottoni  Angela Crobe  Anna Barra Caracciolo  Enzo Funari 《Pest management science》2001,57(12):1135-1141

The metabolites of pesticides can contaminate groundwater and pose a risk to human health when this water is used for drinking. This paper reports the results of a laboratory study on aldicarb and its main metabolites, aldicarb sulfone and aldicarb sulfoxide. Aldicarb and its metabolites showed K_oc values (6–31) which were lower than that of atrazine (55), indicating that they are very mobile in soil. They are less persistent than atrazine (DT₅₀ = 25 days), with DT₅₀ values from less than 1 day and up to 12 days. Aldicarb behaved as a non‐leacher, whereas its metabolites clearly showed the characteristics of leachers. Aged residue leaching experiments showed that aldicarb can occur at high concentrations in the leachate, together with its two metabolites. The leachate composition depends on the incubation time of the parent compound. Aldicarb and its metabolites can form various mixtures in groundwater on the basis of the time elapsing between the application of the insecticide and the first significant rainfall. This study confirms the characteristics of contaminants of aldicarb and especially its metabolites, as reported in the literature. © 2001 Society of Chemical Industry  相似文献