Effects of beneficial bacteria on biomass,photosynthetic parameters and cell composition of the microalga Muriellopsis sp. adapted to grow in seawater     

Paola Marticorena  Leonel Gonzalez  Carlos Riquelme  Fernando Silva Aciares 《Aquaculture Research》2020,51(9):3609-3622

The present study evaluated the effects of five bacterial isolates from the microalga Muriellopsis sp. on the microalgal biomass concentration (g/L), specific growth rate (K), photosynthetic quantum yield (QY), non‐photochemical quenching (NPQ), maximum relative electron transport rates (rETR max) and levels of nutritional components such as carbohydrates, lipids, proteins and carotenoids in Muriellopsis sp. cultivated in seawater under indoor and outdoor conditions. The bacterial strains were Microbacterium sp. Dom 1; Pseudomonas sp. Dom2; Microbacterium sp. Dom 3; Rhizobium sp. Dom 4; and Dietzia sp. Dom 5, as well as the Pseudoalteromonas sp. (SLP1), a bacterium known to promote the growth of microalgae. At the laboratory level, inoculation of the strains SLP1, Dom 1, Dom 3 and Dom 5 resulted in higher specific growth rates and biomass of the microalga. Subsequently, the best results were obtained with a combination of the selected bacterial strains (Dom1, Dom 3, Dom5 and SLP1) under outdoor culture conditions improved the biomass, proteins, carbohydrates and total carotenoids in 22.12%, 48.28%, 19,25% and 48.27%, respectively, compared to cultures without the incorporation of the selected bacteria (control), while no effects were observed on the photosynthetic parameters. Thus, it was demonstrated that positive associations of the selected bacterial strains played an important role in the production of Muriellopsis sp. acclimatized to grow in seawater. This is of crucial importance, especially in desert areas, where solar radiation is high and freshwater is a limited resource.  相似文献   

Characterization of a feline homologue of the alphaE integrin subunit (CD103) reveals high specificity for intra-epithelial lymphocytes     

Woo JC  Roccabianca P  van Stijn A  Moore PF 《Veterinary immunology and immunopathology》2002,85(1-2):9-22

The characteristics of a feline homologue of the alphaE integrin (CD103), defined by two murine monoclonal antibodies, Fe7.1B8 (IgG1) and Fe7.2D8 (IgG1), are described. These antibodies recognized 75% of intra-epithelial (range 59-88%) and 40% of lamina proprial (range 28-46%) T cells of the intestinal mucosal tissue of the small intestine in contrast with approximately 2% of peripheral blood lymphocytes. Both antibodies immunoprecipitated a 180 kDa protein from biotinylated feline intra-epithelial mucosal leukocytes consistent with the alphaE integrin subunit in conjunction with a 120 kDa protein consistent with the beta7 subunit. The nucleotide sequence of feline alphaE integrin, generated from molecular cloning of the feline alphaE encoding cDNA, is also reported. This feline molecule shares 72% sequence homology with human and 69% homology with murine and rat counterparts. Homology includes the presence of an X (extra) domain, that appears unique to alphaE molecules as described for human, rat and mouse, as well as areas of homology common to other alpha integrins. Of note is a typical I (inserted) domain, the presence of seven repeat regions, and highly conserved sequences in the cytoplasmic tail. Transfection studies demonstrated that both antibodies recognized an extracellular component which encompassed the X and I domains of the cloned alphaE integrin subunit. These studies demonstrate that the pattern of tissue distribution, biochemical characteristics, and cDNA sequence of the feline alphaE integrin subunit are largely similar to that described for other species.  相似文献   

A rapid and simple method for the separation of pure lymphocytes from horse blood     

Zizzadoro C  Belloli C  Badino P  Ormas P 《Veterinary immunology and immunopathology》2002,89(1-2):99-104

A method for the separation of pure and viable lymphocytes and granulocytes from the same blood sample in horses was reported. By centrifuging equine heparinized blood at 100 xg for 10 min at room temperature (r.t.), the resulting supernatant plasma was an almost pure (97.71 +/- 0.30%; n = 15) suspension of highly viable (98.72 +/- 0.28%) lymphocytes. When sodium citrate was used as an anticoagulant, lymphocyte suspensions collected in the same manner showed lower purity (87.89 +/- 1.59%; n = 9) and higher yields (56.56 +/- 3.89%, n = 9 versus 36.11 +/- 2.23%, n = 15). Where needed, a further centrifugation at 250 xg for 3 min (r.t.) of heparinized lymphocyte preparations removed an average of 87.39% (n = 15) contaminating platelets. A suspension of 85.96 +/- 2.20% pure granulocytes (93.23 +/- 1.74% neutrophils; n = 14) with minimal contamination by erythrocytes and high viability (93.11 +/- 1.26%) was obtained by performing a flash red blood cell lysis on the white-greyish layer resulting from the centrifugation of the heparinized blood samples. Among the several methods available, the procedure described herein is easy, rapid, cheap and reproducible.  相似文献   

Antioxidant systems and lymphocyte proliferation in the horse,sheep and dog     

Chiaradia E  Gaiti A  Scaringi L  Cornacchione P  Marconi P  Avellini L 《Veterinary research》2002,33(6):661-668

To better define the species-specific antioxidant systems and to ascertain the influence of the intracellular redox status on the immune system of different animal species, we determined lymphocyte glutathione peroxidase (GSHPx) activity, plasmatic glutathione levels (GSH) and the effect of H2O2 on the responsiveness of lymphocytes to proliferative stimuli. Among the three species considered, sheep presented the lowest plasmatic GSH and the highest lymphocyte GSHPx activity. On the contrary, dogs showed an inverted pattern (high GSH - low GSHPx). Horses displayed intermediate values for both parameters analysed. The effect of H2O2 on the proliferative capacity of lymphocytes was the same for all three species; the 200 microM dose in particular was strongly inhibiting. Each species, however, showed different rates of inhibition: sheep exhibited the highest sensitivity to the antiproliferative effect of H2O2. Our results confirmed that high H2O2 concentrations (200 microM) are noxious for the cellular functions of all animals; however this effect is mediated by a rigorously species-specific relationship between the intracellular reactive oxygen species (ROS) and the molecular systems involved in cell proliferation.  相似文献   

Effect of 1-24ACTH administration on sheep blood granulocyte functions     

Paltrinieri S  Panelli S  Comazzi S  Sartorelli P 《Veterinary research》2002,33(1):71-82

The objective of the present study was to determine the efficiency of blood neutrophils (PMN) taken from sheep during acute stress. Ten healthy Charolle sheep were sampled before treatment (T0) and 1 (T1), 2 (T2), 24 (T24) and 48 (T48) hours after 1-24ACTH administration. Ten sheep serving as the controls were sampled at the same time intervals, using saline solution instead of 1-24ACTH. At each time sampling, rectal temperature, heart rate, cortisol, glucose, non-esterified fatty acids (NEFA), total and differential leukocyte counts were evaluated. PMN were isolated after centrifugation of whole blood and hypotonic lysis of RBC. Chemotaxis was evaluated on a modified Boyden chamber using a nitrate cellulose filter and both Zymosan activated serum (ZAS) and interleukin-8 (IL-8) as chemoattractants. Phagocytosis was measured using both non-opsonized latex beads and fluoresceinated yeasts opsonized with homologous serum. Superoxide (O(-)2) production was evaluated by measuring superoxide dismutase-inhibitable reduction of ferricytochrome C, and adherence by a colorimetric assay of acid phosphatase activity of adherent cells. The administration of 1-24ACTH induced an acute stress reaction, indicated by the presence of clinical, biochemical and hematological changes. Adherence significantly increased from T0 to T2 in treated sheep. This might be responsible for the depression of non-specific immunity in stressed animals. Studies using stressors other than 1-24 ACTH are needed to verify the influence of other components of the stress reaction on PMN functions.  相似文献   

Feline leishmaniosis due to Leishmania infantum in Italy     

Poli A  Abramo F  Barsotti P  Leva S  Gramiccia M  Ludovisi A  Mancianti F 《Veterinary parasitology》2002,106(3):181-191

A case of leishmaniosis in domestic cats (Felis catus domesticus) is described. The subject showed a nodular lesion on the eyelid. The diagnosis was achieved by serological, parasitological, and light and electron microscopic investigations. By molecular techniques the aetiological agent was identified as belonging to Leishmania infantum, the species implicated in human and canine leishmaniosis in southern Europe. A preliminary study on the prevalence of asymptomatic feline leishmaniosis, performed in the areas where the infected cat was identified, revealed a low seroprevalence of infection: only 1 (0.9%) of the 110 cat sera examined by indirect fluorescent antibody test was positive for anti-Leishmania antibodies. Because clinical signs in feline leishmaniosis are unspecific and similar to those observed in other diseases commonly found in this species, leishmaniosis must be added to the differential diagnosis by feline veterinary practitioners and adequate serologic and histopathologic investigations must be performed in endemic areas.  相似文献   

Suppression of feed intake after parenteral administration of D-beta-hydroxybutyrate in pygmy goats     

Rossi R  Dörig S  Del Prete E  Scharrer E 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2000,47(1):9-16

The effect of intraperitoneal injection of D,L- or D-beta-hydroxybutyrate on feed intake and plasma metabolites was investigated in pygmy goats. The combined intraperitoneal injection of D,L-beta-hydroxybutyrate or D-beta-hydroxybutyrate (15 mmol/kg0.75) and 1,3-butanediol (6.6 mmol/kg0.75), a ketogenic substrate, decreased cumulative food intake while the same dose of 1,3-butanediol or DL-beta-hydroxybutyrate alone had no effect. The decrease in food intake after combined injection of D-beta-hydroxybutyrate and 1,3-butanediol was characterized by a significant decrease in meal frequency and a prolongation of the latency to eat. The hypophagic effect of the combined injection of D-beta-hydroxybutyrate and 1,3-butanediol was significant for 8 h, while the hypophagia after D,L-beta-hydroxybutyrate and 1,3-butanediol was significant for only 2 h after injection. Injection of D,L-beta-hydroxybutyrate increased plasma D-beta-hydroxybutyrate levels to 0.55 mmol/l and decreased plasma free fatty acids. Addition of 1,3-butanediol (6.6 mmol/kg0.75) to the injection increased plasma D-beta-hydroxybutyrate level up to 0.75 mmol/l. The results show that parenteral administration of D-hydroxybutyrate reduces feed intake in pygmy goats and that the hypophagia appears to be related to the amount of D-beta-hydroxybutyrate injected. The hypophagia seems to be related to elevated plasma D-beta-hydroxybutyrate concentration, and the threshold concentration appeared to be about 0.7 mmol/l under the experimental conditions of this study.  相似文献   

Forecasting Infections of the Leaf Curl Disease on Peaches Caused by Taphrina deformans     

S. Giosuè  G. Spada  V. Rossi  G. Carli  I. Ponti 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(6):563-571

An Israeli model forecasting leaf curl disease on peaches caused by Taphrina deformans was validated in the Emilia-Romagna region of northern Italy, during a three-year period (1996–1998), in 13 cases (year × location × cultivar). When the peach trees are susceptible to infection, the model uses mathematical functions to calculate the risk of infection on the basis of weather conditions (daily rainfall greater than 10mm, and maximum air temperature greater than 5°C), and it forecasts periods of possible symptom appearance based on the length of incubation. Peach trees became susceptible to infection between the end of January and mid March, when the first leaf buds attained phenological stage C, i.e. appearance of leaf apex. The trees remained susceptible for at least 9 weeks: the last infection occurred in mid-May.Since most of the leaf curl onsets observed in the orchards fell within the range of model forecasts, the model proved to be accurate in signalling both the first seasonal infection and repeated infections during the primary inoculum season. Few errors occurred, caused either by conditions of rainfall and temperature lower than the thresholds fixed in the model, or by discrepancies between forecast and actual length of incubation. Infection occurred also at 3.1–3.5°C, and with 9.6mm rainfall. Thus, thresholds should not be accepted too rigorously, and perhaps temperature should not be considered as a limiting factor for infection under the conditions of the present work. The length of incubation showed high variability: it was 23 days long on average, with a 95% confidence interval ranging from 20 to 27 days, and extreme values of 9 and 33 days.  相似文献   

A decision support system for Fusarium head blight on small grain cereals*     

V. Rossi  S. Giosu  V. Terzi  D. Scudellari 《EPPO Bulletin》2007,37(2):359-367

A DSS was elaborated to determine the level of risk for mycotoxin accumulation in small grain cereals during the growing season and to support different management actions along the cereal production chain. The DSS is based on a discriminant analysis carried out on a representative data set on the presence of deoxinivalenol in winter‐sown bread wheat, durum wheat and barley grown in different areas of Emilia‐Romagna (northern Italy) between 2002 and 2004. Three to five levels of proneness to Fusarium head blight were defined for six influencing factors (weather conditions, growing area, host species and variety, previous crop and type of soil tillage before sowing), each level having its own coefficient, and linearly combined in an equation of risk (R). A value of R can be then calculated for any plot using information at both regional (weather conditions and growing area) and plot‐specific (host and cropping practices) levels. R ranges between −3.86 and 4.43 with four risk levels: low (R  –2), intermediate (–2 < R  –0.44), high (–0.44 < R  1.2), and very high (R > 1.2). Different management actions are suggested based on the level of R of the plot. The DSS was satisfactorily validated using data from many plots other than those used in its elaboration.  相似文献   

COMBINATION OF RADIATION THERAPY AND FIROCOXIB FOR THE TREATMENT OF CANINE NASAL CARCINOMA          下载免费PDF全文

Simona Cancedda  Silvia Sabattini  Giuliano Bettini  Vito F. Leone  Paola Laganga  Federica Rossi  Rossella Terragni  Giacomo Gnudi  Massimo Vignoli 《Veterinary radiology & ultrasound》2015,56(3):335-343

Carcinomas represent two‐thirds of canine nasosinal neoplasms. Although radiation therapy (RT) is the standard of care, the incidence of local recurrence following treatment is high. Cyclooxygenase‐isoform‐2 (COX‐2) is expressed in 71–95% of canine nasal carcinomas and has been implicated in tumor growth and angiogenesis. Accordingly, COX‐2 inhibition seems rational to improve outcome. Dogs with histologically confirmed, previously untreated nasal carcinomas were randomized to receive the combination of a selective COX‐2 inhibitor (firocoxib) and palliative RT (Group 1) or RT and placebo (Group 2). Patients were regularly monitored with blood tests, urinalysis, and computed tomography. Pet owners were asked to complete monthly a quality‐of‐life questionnaire. Twenty‐four dogs were prospectively enrolled. According to Adams modified system, there were five stage 1, five stage 2, three stage 3, and 11 stage 4 tumors. Two dogs had metastases to regional lymph nodes. Median progression‐free interval and overall survival were 228 and 335 days in Group 1 (n = 12) and 234 and 244 days in Group 2 (n = 12). These differences were not statistically significant. The involvement of regional lymph nodes was significantly associated with progression‐free interval and overall survival (P = 0.004). Quality of life was significantly improved in Group 1 (P = 0.008). In particular, a significant difference was observed for activity and appetite. Although not providing a significant enhancement of progression‐free interval and overall survival, firocoxib in combination with RT is safe and improved life quality in dogs with nasal carcinomas.  相似文献