Fish and seafood traceability based on AFLP markers: Elaboration of a species database     

Milena Maldini  Gloria González Fortes  Riccardo Papa 《Aquaculture (Amsterdam, Netherlands)》2006,261(2):487-494

Several sociological, health and conservation arguments request a correct labelling of seafood products. Nowadays, molecular genetics is a useful tool for food chain traceability, particularly in regards to species identification. Among the variety of PCR-based molecular markers, AFLPs (Amplified Fragment Length Polymorphisms) have recently been used to investigate genomes of different complexities. This paper assesses the potential use of the AFLP technology to determine fish and seafood species in processed commercial products and domestic stocks. In particular a species database of fish, molluscs and crustaceans has been created with the aim to identify species of origin of seafood products by previously defined AFLP patterns. Different EcoRI and TaqI primer combinations were selected from 20 screened combinations in relation to the total number of detected fragments and polymorphic ones. Most informative combinations were E32/T32, E32/T33, E33/T33, E33/T37, E33/T38, E40/T33, E40/T37, E42/T32, E42/T37. The comparison of informative markers between unknown frozen or fresh products and reference samples has enabled the accurate identification of 32 different species. The taxonomic characterization has been performed either at the species or at the population level depending on the number of available individuals. AFLP variation at the population level is particularly helpful for the stock traceability of domestic strains. Size homoplasy was also investigated in one species to assess the rate of non-homologous comigrating fragments and to detect additional polymorphic markers to be used in stock identification. Results of Band Sharing Index (BSI) and percentage of polymorphic fragments are presented and are discussed in relation to the wide applicability of AFLPs both for fish and seafood safety and authenticity testing in such fields as food traceability and restocking management. The database, available upon request at nonnis@biol.unipr.it, will be continuously updated.  相似文献   

Effect of Eucalyptus camaldulensis amendment on soil chemical properties,enzymatic activity,Acacia species growth and roots symbioses     

Abdoulaye Soumare  Anicet Manga  Saliou Fall  Mohamed Hafidi  Ibrahima Ndoye  Robin Duponnois 《Agroforestry Systems》2015,89(1):97-106

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Ecological diversity of sediment rhizobacteria associated with Phragmites australis along a drainage canal in the Yellow River watershed     

Borruso  Luigimaria  Esposito  Alfonso  Bani  Alessia  Ciccazzo  Sonia  Papa  Marianna  Zerbe  Stefan  Brusetti  Lorenzo 《Journal of Soils and Sediments》2017,17(1):253-265

Journal of Soils and Sediments - Spatial distribution on a regional scale of the rhizobacterial communities of Phragmites australis stands was investigated along the Yellow River watershed, China....  相似文献   

Effects of dietary cholesterol on hepatopancreas associated with morphotypic differentiation in male Amazon River Prawns,Macrobrachium amazonicum (Heller, 1862)     

Thalles Fernando Rocha Ruiz  Karina Ribeiro  Carlos Alberto Vicentini  Irene Bastos Franceschini Vicentini  Luciene Patrici Papa 《Aquaculture Research》2019,50(3):862-870

Cholesterol is a necessary element in the diet of prawns and it is involved in the metabolic processes of sexual maturation and synthesis of the molting hormone. Freshwater prawns are not able to synthesise cholesterol from specialised metabolic pathways. The aim of this study was to describe changes in structural and morphometric patterns of hepatopancreas associated with morphotypic differentiation of males Macrobrachium amazonicum when fed with different levels of cholesterol. Considering the four morphotypes of this species, Translucent Claw morphotype specimens were split into experimental units and divided into four groups, fed with different levels of cholesterol (0%, 0.5%, 1%, 2%) for 50 days. They were collected as they reached Green Claw (GC) morphotype, weighed to determine the hepatosomatic index, and the hepatopancreas was processed in histological routine for morphologic and morphometric analysis. In this study, it was possible to observe that the 2% cholesterol treatment exhibited more animals that reached the GC morphotype, and there was no significant weight gain in all treatments. Histologically, B‐cell abundance and hypervacuolized as well as F‐ and R‐cells shortages were observed as the percentage of cholesterol increased. This alteration in cellular profile correlates with the morphometric analyses that exhibited an increase in epithelial area and alterations in the lumen shape. This imbalance observed in the lumen‐epithelium relationship may be associated with a low digestive performance and possibly congested the functions of hepatopancreas. Thus, high doses of cholesterol promoted morphotypic differentiation without weight gain, due to alterations on hepatopancreas.  相似文献