Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis     

Thaís Mendes Sanches Cavalero  Lorenzo Garrido Teixeira M. Segabinazzi  Verônica Flores da Cunha Scheeren  Camila P. Freitas-Dell'Aqua  Frederico Ozanam Papa 《Reproduction in domestic animals》2020,55(12):1808-1811

The present study compared the quality of sperm collected by artificial vagina or pharmacologically induced ejaculation from a 10-year-old thoroughbred stallion with seminal vesiculitis. The pharmacological protocol involved intravenous administration of detomidine (0.01 mg/kg) and oxytocin (20 IU) and successfully induced ejaculation in all attempts of semen collection. Sperm motility, plasma membrane and acrosome integrity (PMAI), reactive oxygen species (ROS) levels, polymorphonuclear neutrophil (PMN) percentage, and bacterial profiles of fresh and cooled semen (5°C for 24 hr) were evaluated. Semen obtained by the pharmacological method presented reduced seminal volume, decreased PMN percentage and superior sperm motility in cooled samples. Moreover, higher PMAI and lower ROS levels were observed in semen collected by the pharmacological method. Therefore, pharmacologically induced ejaculation is an alternative to obtain semen with minimal contamination and with sperm of superior quality and longevity from stallions with seminal vesiculitis.  相似文献   

Issues and challenges in landscape models for agriculture: from the representation of agroecosystems to the design of management strategies     

Sylvain Poggi  Julien Papaïx  Claire Lavigne  Frédérique Angevin  Florence Le Ber  Nicolas Parisey  Benoît Ricci  Fabrice Vinatier  Julie Wohlfahrt 《Landscape Ecology》2018,33(10):1679-1690

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Study of testicular thermoregulation efficiency in stallion with different ages     

C. Ramires-NetoG.A. Monteiro  D.J.Z. DelfiolE.G. Fioratti  J.A. Dell`aqua Jr.F.O. Papa  M.A. Alvarenga 《Journal of Equine Veterinary Science》2012

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Effect of addition of soybean lecithin to Botu-Crio on sperm parameters and fertility rates of frozen equine semen     

A.S. RochaI. Martin  G.A. MonteiroP.N. Guasti  Y.F.R. Sancler-SilvaF.O. Papa 《Journal of Equine Veterinary Science》2012

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Methods of Concentrating Stallion Semen     

Marco Antonio Alvarenga  PhD  Frederico O. Papa  Marcio T. Carmo  Tathiane Kievitsbosch  Maria Manoela B. Castro Chaves  Carlos Ramires Neto 《Journal of Equine Veterinary Science》2012

Removal of excess seminal plasma is sometimes necessary to increase the quality and the longevity of cooled equine semen; moreover, this procedure is an indispensable step aiming to concentrate the sperm cells before freezing equine semen. Typically, the removal of seminal plasma is achieved by centrifugation; however, studies have shown that the force and duration of centrifugation can damage sperm cells and reduce the sperm recovery rate. Recently, new methodologies, such as cushion and filtration, have been described that aim to decrease the mechanical damage of centrifugation to sperm cells. This study aims to compare different methods for concentrating stallion semen.  相似文献   

Type 1 diabetes mellitus and hyperadrenocorticism in a ferret     

Boari  A.  Papa  V.  Di Silverio  F.  Aste  G.  Olivero  D.  Rocconi  F. 《Veterinary research communications》2010,34(1):107-110

Diabetes mellitus (DM) was diagnosed in a 6-year-old neutered male ferret with polyuria/polydipsia, symmetrical alopecia, and weight loss. Laboratory tests revealed severe hyperglycemia, glucosuria, and increased steroid hormone profile. Abdominal ultrasound revealed a bilateral enlargement of the adrenal glands. Significant clinical improvement was achieved with insulin- and leuprolide acetate-based therapy. After 2 months of therapy, the ferret showed a severe ketoacidosis, and the owner decided to euthanize the animal. Histological findings revealed carcinoma of the left adrenal cortex and cortical hyperplasia of the right adrenal gland. Moderate, chronic, and active pancreatitis with a marked decrease in the number of β-cells was also present. This is the first reported case of type 1 DM associated with hyperadrenocorticism and chronic pancreatitis in a ferret.

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A long-term study of weed flora shifts in different tillage systems   总被引：1，自引：0，他引：1  

D Tuesca  E Puricelli  & J C Papa 《Weed Research》2001,41(4):369-382

Knowledge of the long-term effects of tillage on weed flora will provide useful information to improve weed management in agroecosystems. Field studies were conducted from 1991 to 1997 to evaluate the effects of tillage systems on weed density and species composition before control methods in rotations including wheat ( Triticum aestivum L.), soyabean [ Glycine max (L.) Merr.] and maize ( Zea mays L.). In wheat, annual broad-leaved species showed higher populations in conventional tillage in 4 out of 6 years, and grassy annuals and perennial species showed an erratic response with tillage systems. In summer crops, broad-leaved populations were higher under conventional tillage than non-tillage for the last 5 years in the wheat/soyabean rotation and for the last 4 years in the maize/soyabean rotation. The weed spectrum changed rapidly in non-tillage plots. With time, in the absence of tillage, grassy annual populations increased in the maize/soyabean rotation, and wind-dispersed weed populations increased in the wheat/soyabean rotation. Perennial weeds showed an inconsistent behaviour in relation to tillage systems in the maize/soyabean rotation.  相似文献   

Equine Perineal and Vulvar Conformation Correction Using a Modification of Pouret's Technique     

Frederico O. Papa  Cely M. Melo  Gabriel A. Monteiro  Patricia M. Papa  Priscilla N. Guasti  Rosiara Rosária D. Maziero  Ana Augusta P. Derussi  Luis Carlos O. Magalhães  José Carlos Martin  Ian Martin 《Journal of Equine Veterinary Science》2014

An incompetent vulvar seal leads to reproductive failure, and a surgical intervention might be required. The present paper describes modifications to Pouret's surgery. We suggest the use of a simple interrupted vertical mattress suture, which avoids seroma. Eighteen Brazilian Jumping Horse mares, older than 20 years and barren for 3-5 consecutive years, underwent modified Pouret's surgery. A horizontal skin incision of 3-4 cm was made half way between the anus and upper commissure of the vulva. The submucosal and connective tissue were dissected, and the rectovaginal shelf was split horizontally by sectioning the muscular and ligamentous connections between the anus, vulva, caudal portion of the rectum, and vagina until the vulva was oriented vertically. The wound was changed from a horizontal plane to a vertical plane by placing the suture vertically using approximately eight interrupted U sutures distributed in two layers with polyamide thread. The modified Pouret's surgical technique provided a perfect coaptation of the vulvar lips and a correct perineal position. Those mares that presented with horizontally tipped vulvar lips due to advanced age and stretching of the pelvic tissues by multiple foaling had their vulvas replaced. Also, the surgical procedure was easy to perform. As to fertility, of the 18 initial mares, 14 were inseminated, and all became pregnant. Thus, it was possible to conclude that the anatomical changes performed throughout the surgical procedure predisposed to a better vulvar coaptation, correcting the pneumovagina.  相似文献   

Sperm parameters and fertility of stallion semen cooled with different extenders     

P.M. PapaC. Ramires-Neto  G.A. MonteiroY.F.R. Sancler-Silva  H.L. ResendeC.P. Freitas-Dell`aqua  J.A. Dell`aqua Jr.M.A. Alvarenga  F.O. Papa 《Journal of Equine Veterinary Science》2014

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An approach to rescue the fertility of stallions with a high level of hemospermia     

Luiz Roberto P. Andrade Junior  Lorenzo G. T. M. Segabinazzi  Sidnei N. Oliveira  José Antonio Dell'Aqua Jr  Frederico O. Papa 《Reproduction in domestic animals》2020,55(9):1258-1262

A high amount of blood and not the mere presence of blood in equine semen impacts fertility. The aim of this study was to develop an approach to rescue the fertility of stallions with high hemospermia levels. Semen from 15 stallions was divided into four experimental groups: (a) Control—pure raw semen, (b) WB50—50% (v/v) whole blood added into semen, (c) E1—WB50 extended in a 1:1 (v/v) ratio with milk-based extender and (d) E2—WB50 extended in a 2:1 ratio with milk-based extender. Sperm kinetics, plasma membrane integrity (PMI), lipid peroxidation (PER) and intracellular superoxide (O₂) production were immediately evaluated. Four cycles of 20 mares were randomly assigned to the experimental groups. Mares were bred with an insemination dose of 1 billion total sperm and pregnancy was diagnosed 14 days after ovulation. Sperm kinetics could not be evaluated in the WB50 samples. Total motility was lower (p < .05) in E1 than in CT and E2 samples. Progressive motility decreased (p < .05) with an increase in the percentage of blood in the samples. The PMI and PER did not differ between groups (p > .05); however, O₂ production was higher (p < .05) in WB50 than in E2 samples, while the values were intermediate (p > .05) for CT and E1 samples. The control (90%) and E2 (90%) groups had superior (p < .05) fertility than the others (WB50—0% and E1—25%). It was concluded that sperm motility and fertility of semen with a large amount of blood can be rescued by dilution with a 2:1 extender:semen ratio using a milk-based extender.  相似文献