Classical swine fever in Victorian domestic pigs: evidence of disease freedom     

JC Hunnam  KM Moore  P Daniel  MA Stevenson  SE Salmon 《Australian veterinary journal》2019,97(11):447-451

  相似文献   

A preliminary survey of Chlamydia psittaci genotypes from native and introduced birds in New Zealand     

KR Gedye  M Fremaux  JC Garcia-Ramirez 《New Zealand veterinary journal》2018,66(3):162-165

AIM: To describe the Chlamydia psittaci genotypes in samples from native and introduced birds from New Zealand by analysis of the sequence variation of the ompA gene.

METHODS: DNA was extracted from samples collected from a non-random sample of birds; either swabs from live asymptomatic birds or birds with clinical signs, or formalin-fixed, paraffin-embedded (FFPE) samples from historical post-mortem cases. The presence of C. psittaci in all samples had been confirmed using a quantitative PCR assay. The C. psittaci ompA gene was amplified and sequenced from samples from 26 native and introduced infected birds comprising 12 different species. These sequences were compared to published available C. psittaci genotypes.

RESULTS: Genotypes A and C of C. psittaci were identified in the samples. Genotype A was identified in samples from nine birds, including various native and introduced species. Genotype C was identified in samples from 16 different waterfowl species, and a mixed infection of both genotypes was found in a kaka (Nestor meridionalis). In native birds, C. psittaci infection was confirmed in seven new host species.

CONCLUSIONS AND CLINICAL RELEVANCE: Two genotypes (A and C) of C. psittaci were found in samples from a wider range of both native and introduced species of birds in New Zealand than previously reported. Both genotypes have been globally associated with significant disease in birds and humans. These initial results suggest the host range of C. psittaci in New Zealand birds is under-reported. However, the prevalence of C. psittaci infection in New Zealand, and the associated impact on avian and public health, remains to be determined. There are biosecurity implications associated with the importation of birds to New Zealand if there is a limited diversity of C. psittaci genotypes present.  相似文献   

Pyometra and unilateral uterine horn torsion in a sheep          下载免费PDF全文

JM Castillo  JC Dockweiler  SH Cheong  M Diel de Amorim 《Reproduction in domestic animals》2018,53(1):274-277

A 13‐year‐old Romanov sheep presented for evaluation of vaginal discharge, depression and anorexia. Blood work demonstrated mature neutrophilia and marked hyperlactatemia. Transrectal and transabdominal ultrasound revealed echogenic fluid (pus) within the uterus. Purulent uterine fluid culture yield Escherichia coli. A diagnosis of pyometra was made. After medical treatment without clinical improvement, an exploratory laparotomy was performed, and a 360‐degree uterine left horn torsion was identified with the pyometra and multiple corpora lutea on both ovaries. Ovariohysterectomy was performed and the ewe recovered uneventfully.  相似文献   

Antihyperlipidemic Effects of Sour Cherries Characterized by Different In Vitro Antioxidant Power and Polyphenolic Composition     

Nóra Papp  Anna Blázovics  Hedvig Fébel  Sofía Salido  Joaquín Altarejos  Erzsébet Fehér  Ibolya Kocsis  Klára Szentmihályi  László Abrankó  Attila Hegedűs  Éva Stefanovits-Bányai 《Plant foods for human nutrition (Dordrecht, Netherlands)》2015,70(4):408-413

  相似文献   

Changes in European eel ovary development and body and ovary chemistry during stimulated maturation under controlled conditions: preliminary data     

Joanna Nowosad  Dariusz Kucharczyk  Joanna Łuczyńska  Katarzyna Targońska  Tomasz Kajetan Czarkowski  Maria Biłas  Sławomir Krejszeff  László Horváth  Tamás Müller 《Aquaculture International》2015,23(1):13-27

  相似文献   

Internal standardization--atomic spectrometry and geographical pattern recognition techniques for the multielement analysis and classification of Catalonian red wines     

Iglesias M  Besalú E  Anticó E 《Journal of agricultural and food chemistry》2007,55(2):219-225

Major and minor (K, P, Ca, Mg, Na, Fe, Mn, Zn, and Sr) and trace (Ba, Ni, Pb, V, Co, Cd, and Sb) elements from wine samples from the Denomination of Origin (DO) Empordà-Costa Brava (Catalonia, Spain) were analyzed by inductively coupled plasma atomic emission spectrometry (ICP-AES) and mass spectrometry (ICP-MS) respectively. Previously, a comparison of different calibration methodologies and sample digestion treatments had been carried out using ANOVA statistical tool. The obtained results demonstrated that internal standardization provides reliable results with the advantage that no further manipulation of the sample is needed. A principal component analysis of the concentration data was performed to differentiate the samples of DO Empordà-Costa Brava from wine samples from other wine-producing regions in Spain (i.e., Penedès, Somontano, and Rioja). It was found that Sr and Ba contents discriminate the two DO groups. Moreover, a discriminant analysis function involving both variables distinguishes the two groups with a 100% classification rate. At the level of the leave-one-out cross-validation, all of the Empordà-Costa Brava samples were well classified, whereas the other DOs presented two borderline misclassifications.  相似文献   

Postmortem diagnosis of preclinical and clinical scrapie in sheep by the detection of disease-associated PrP in their rectal mucosa     

González L  Dagleish MP  Bellworthy SJ  Sisó S  Stack MJ  Chaplin MJ  Davis LA  Hawkins SA  Hughes J  Jeffrey M 《The Veterinary record》2006,158(10):325-331

Samples of tissue from the central nervous system (cns), the lymphoreticular system (lrs) and the rectal mucosa of a large number of scrapie-exposed sheep, with and without signs of clinical disease, were examined immunohistochemically for evidence of disease-associated prion protein (PrP(d)). The rectal mucosa has received almost no attention so far in scrapie diagnosis, despite its abundant rectoanal mucosa-associated lymphoid tissue, and its accessibility. The scrapie-confirmed cases included 244 with clinical disease, of which 237 (97.1 per cent) were positive in the rectal mucosa, and 121 apparently healthy sheep, of which 104 (86 per cent) were positive in the rectal mucosa. PrP(d) was detected in 86.4 to 91.5 per cent of the other lrs tissues of the healthy sheep examined and in 77.7 per cent of their cns tissues. The stage of infection, therefore, affected the probability of a positive result in the rectal mucosa, whereas the breed, PrP genotype, age and sex had little or no independent effect. Accumulations of PrP(d) were observed in the rectal mucosa and other lrs tissues of vrq/arr sheep with preclinical and clinical scrapie, albeit with a lower frequency and magnitude than in sheep of other PrP genotypes. Western immunoblotting analyses of samples of rectal mucosa gave the characteristic PrP glycoprofile, with a sensitivity similar to that of immunohistochemistry.  相似文献   

Evaluation of a spray of permethrin and pyriproxyfen for the protection of dogs against Phlebotomus perniciosus     

Molina R  Miró G  Gálvez R  Nieto J  Descalzo MA 《The Veterinary record》2006,159(7):206-209

Dogs are the main domestic reservoir of Leishmania infantum in the Old World (Leishmania chagasi in the New World) a parasite responsible for many cases of human visceral leishmaniasis in both endemic and non-endemic regions. One strategy for the control of leishmaniasis would be to prevent dogs from being bitten by sandflies, the vector of leishmaniasis. This study was designed to assess the efficacy of spraying a combination of permethrin and pyriproxyfen on to dogs artificially exposed to sandflies. Two groups of four male dogs, one of them treated and the other left untreated as controls, were exposed for one hour to 100 female sandflies seven days before the treatment, on the day of treatment and seven, 14, 21, and 28 days later. After each exposure, sandflies were collected, counted and scored. The prevention of sandfly bite was calculated by measuring the number of fed sandflies (dead and alive) after treatment. In this experimental assay, the repellent effect of the treatment against sandfly bites after 21 days was 71.4 per cent, but the insecticidal effect was only 7.2 per cent.  相似文献   

Bovine tuberculosis in wild boar (Sus scrofa), red deer (Cervus elaphus) and cattle (Bos taurus) in a Mediterranean ecosystem (1992-2004)     

Hermoso de Mendoza J  Parra A  Tato A  Alonso JM  Rey JM  Peña J  García-Sánchez A  Larrasa J  Teixidó J  Manzano G  Cerrato R  Pereira G  Fernández-Llario P  Hermoso de Mendoza M 《Preventive veterinary medicine》2006,74(2-3):239-247

  相似文献   

Detection of Mycoplasma bovis with an improved pcr assay     

Tenk M  Bálint A  Stipkovits L  Biró J  Dencso L 《Acta veterinaria Hungarica》2006,54(4):427-435

A Mycoplasma bovis species-specific PCR assay has been developed with improvement of a previously described method (Ghadersohi et al., 1997). This test and its semi-nested version (Hayman and Hirst, 2003) did not function at all in our hands. A new reverse primer (Mbr2) was designed using previously published sequence data. For testing specificity, DNA was extracted from the most frequently occurring mycoplasma species and bacteria of bovine origin. The new PCR detected only Mycoplasma bovis. Moreover, no cross-reaction was observed with the genetically closest relative species, M. agalactiae. The target organism could be detected in a dose as low as 150 CFU ml(-1) in broth cultures using ethidium-bromide-stained agarose gels.  相似文献