The application of tannic acid to the elimination of egg stickiness at varied moments of the egg swelling process in pikeperch,Sander lucioperca (L.)          下载免费PDF全文

Daniel Żarski  Sławomir Krejszeff  Dariusz Kucharczyk  Katarzyna Palińska‐Żarska  Katarzyna Targońska  Krzysztof Kupren  Pascal Fontaine  Patrick Kestemont 《Aquaculture Research》2015,46(2):324-334

The aim of this article was to analyse the process of pikeperch, Sander lucioperca, egg swelling and to apply tannic acid to eliminate egg stickiness at different moments of the swelling process on artificially obtained eggs. The first experiment involved observation of egg swelling process and the second determined the effect of temperature (12, 14 and 16°C) on the egg swelling rate. The third experiment involved elimination of egg stickiness in a tannin solution (0.75 g L^?1) where eggs were submerged in a solution for 0.5, 1, 2 and 5 min – 5, 10, 15, 20, 25 and 30 min following gamete activation. The results indicate that the pikeperch egg swelling process lasts 30 min. It was found that the temperature did not affect the process duration. The results of the third experiment showed that the effectiveness of tannic acid application in egg stickiness elimination increases with time. The best result was obtained in groups of eggs submerged for 1 and 2 min (86.5% and 80.5% of larvae were obtained respectively) 30 min following the gamete activation. The results presented in this study for the first time indicate the possibility of highly effective procedure of egg stickiness elimination with tannic acid in pikeperch aquaculture.  相似文献   

Off-Season Spawning of Eurasian Perch Perca Fluviatilis     

Herve Migaud  Jean-Noel Gardeur  Patrick Kestemont  Pascal Fontaine 《Aquaculture International》2004,12(1):87-102

This study was designed to determine the effects of the selection for body weight and of two final warming durations during an out-of-season reproductive cycle on gonad recrudescence, plasma steroid levels and spawning in Eurasian perch Perca fluviatilis males and females. Two different bodyweight classes (200–250 and 350–400 g) and two different warming periods (1 or 2 months) were studied.At the end of the chilling period, a significant effect of the selection for body weight was observed on gonad development whereas fish were the same age. According to higher levels in testosterone (12 ng mL^–1), a better development was recorded for smaller breeders (P < 0.05). A short water warming period up to 14°C (1 month v.s. 2 months) allowed higher rates of females with completed gonadogenesis (33–38% v.s. 7–8%) and spawning (31–33% v.s. 0%) (P < 0.05). Spontaneous out-of-season spawning (17 ribbons) and larvae were obtained. Relative fecundity was about 100 eggs g^–1 of female body weight. Five ribbons were partly fertilized with a rate ranging from 5 to 90%. These studies have shown that delayed spawning in Eurasian perch can be obtained by a temperature manipulation, but results are not sufficient because of low stimulation rate of females, low spawning rates and high heteroge-neity of fertilization rates. The effects of other environmental factors like photoperiod should be tested in further experiment to improve the protocol inducing out-of-season spawning production.  相似文献   

Baker's Yeast as a Potential Substitute for Live Algae in Aquaculture Diets: Artemia as a Case Study   总被引：4，自引：0，他引：4  

Peter  Coutteau  Patrick  Lavens Patrick  Sorgeloos 《Journal of the World Aquaculture Society》1990,21(1):1-9

In this study baker's yeast was evaluated as a substitute for live Dunuliella tertiolecta algae in the culture of the brine shrimp Artemia . Consumption of fresh baker's yeast resulted in poor growth and survival of brine shrimp. However, the nutritional value of the yeast sigaificantly improved after complete removal of the yeast cell wall by enzymatic treatment. Baker's yeast was also made digestible for Artemia by simple chemical treatment which did not reduce rigidity of the yeast cell. The external mannoprotein layer of the yeast cell wall is probably the major barrier to digestion by Artemia . Chemically treated baker's yeast offers promising possibilities as a substitute for algal feeds in aquaculture.  相似文献   

Effect of carbon/nitrogen ratio manipulation in feed supplements on Artemia production and water quality in solar salt ponds in the Mekong Delta,Vietnam     

Lulijwa Ronald  Gilbert Van Stappen  Nguyen Van Hoa  Patrick Sorgeloos 《Aquaculture Research》2014,45(12):1906-1912

The effect of carbon/nitrogen (C/N) ratio manipulation in feed supplements on Artemia production and water quality was investigated in solar salt ponds in the Mekong Delta, Vietnam. It was assumed that development of bacterial bioflocs through C/N manipulation would improve Artemia production and water quality as demonstrated in freshwater and marine aquaculture. Twelve ponds were used for three treatments and the control, in triplicates. Green water (GW) was supplied to all ponds, with the standard Vietnamese procedure of supplying GW and chicken manure (CM) as the control (C/N 1.8). Treatment ponds were supplemented with tapioca (TAP) as carbon source, combined with either CM, pig manure (PM) or rice bran (RB), with C/N ratios of 7.4, 10.5 or 20.1 respectively. After 6 weeks of culture, no single treatment supported both improved water quality and enhanced Artemia production. Overall, improved water quality was observed at C/N 20.1 and higher Artemia production at C/N 7.4. Although external field factors could have interfered with the set‐up, this is the first study on the effect of C/N manipulation in feed supplements in Artemia pond production systems and provides the basis for development of bacterial bioflocs as a technology to improve water quality and Artemia production.  相似文献   

Evaluating foliar nitrogen compounds as indicators of nitrogen status in Prunus persica trees     

Oswaldo A. Rubio-Covarrubias  Patrick H. Brown  Steven A. Weinbaum  R. Scott Johnson  Raul I. Cabrera 《Scientia Horticulturae》2009

Mobile nitrogen (N) forms may be better N indicators of the N status of trees than total nitrogen (TN) due to their higher sensitivity to increasing N supply. A field experiment was carried out over a 3-year period to compare foliar concentrations of total N (TN), soluble N (SN), chlorophyll (Minolta SPAD readings), NH₄–N and NO₃–N as indicators of soil N availability in nectarine, Prunus persica L. Batsch, cv. ‘Fantasia’ (grafted on ‘Nemaguard’ peach, P. persica × P. davidiana) trees. Young trees were exposed to a range of fertilizer-N application rates. Based on correlation analysis, the best association between leaf N compounds with soil N supply and trunk diameter and/or fruit yield was obtained with TN and chlorophyll SPAD readings. Leaf concentrations of mobile N compounds (NH₄–N and NO₃–N) increased more than any other N compound under high N supply; however, their inconsistency among years and low leaf concentration difficult their use as N indicators. The optimum foliar TN for growth decreased with tree age, 4.4%, 3.6% and 3.3% in non-bearing 1-year-old trees, non-bearing 2-year-old trees and 3.3 fruit-bearing 3-year-old trees. The optimum SPAD readings were 40 in 2-year-old trees and 42 in 3-year-old trees. Stable N compounds (TN and chlorophyll SPAD) could be used to N diagnosis in the zone of N deficiency, and soluble N compounds (NH₄–N and NO₃–N) to diagnoses N excess.  相似文献   

The Pelagos Sanctuary for Mediterranean marine mammals     

Giuseppe Notarbartolo‐di‐Sciara  Tundi Agardy  David Hyrenbach  Tullio Scovazzi  Patrick Van Klaveren 《水产资源保护:海洋与淡水生态系统》2008,18(4):367-391

• 1. In February 2002, France, Italy and Monaco agreed to establish an international sanctuary for Mediterranean marine mammals. The resulting Pelagos Sanctuary encompasses over 87500 km² of the north‐western Mediterranean Sea, extending between south‐eastern France, Monaco, north‐western Italy and northern Sardinia, and surrounding Corsica and the Tuscan Archipelago.
• 2. The Pelagos Sanctuary illustrates how the tenets of Marine Protected Area (MPA) design can be reconciled with the dynamic nature of oceanic systems, because its spatial scale was defined by oceanographic and ecological considerations, specifically the location of the Ligurian permanent frontal system.
• 3. By expanding protective measures beyond national waters, the Pelagos Sanctuary also sets a precedent for the implementation of pelagic protected areas in the high seas. The Pelagos Sanctuary will contribute to the conservation of the Mediterranean Sea at two scales: (i) locally, by protecting important cetacean foraging and breeding grounds in the Ligurian Sea, and by providing ‘umbrella’ protection to other marine predators in this area; and (ii) regionally, by empowering other conservation measures, such as the Specially Protected Areas Protocol of the Barcelona Convention and the wider goals of the Agreement on the Conservation of Cetaceans of the Black and Mediterranean Seas (ACCOBAMS).
• 4. However, because few cetacean species are resident within the Sanctuary, their effective long‐term conservation will require large‐scale management and coordinated monitoring throughout the Mediterranean basin.

Copyright © 2007 John Wiley & Sons, Ltd.  相似文献   

Effects of stocking density on the survival, growth, size variation and condition index of juvenile rabbitfish Siganus rivulatus     

I. Patrick Saoud  Joly Ghanawi  Nada Lebbos 《Aquaculture International》2008,16(2):109-116

Spinefoot rabbitfish, Siganus rivulatus, is an economically important species of herbivorous fish that is relatively easy to rear and thus considered to be suitable for aquaculture. Juveniles are generally reared in nursery systems before being stocked into growout cages or ponds. We report here our evaluation of the effects of stocking density on the survival, growth, feed efficiency and condition index of S. rivulatus juveniles in nursery tanks. The experiment was conducted in a recirculating system of twelve 52-l aquaria connected to a biological filter and a sand filter. Juvenile fish (average weight 6.5 g) were stocked into aquaria at four stocking densities (10, 20, 30, and 40 fish/aquarium) with three replicate aquaria per treatment. Diet was provided at 3% body weight daily divided into two feedings. Fish were weighed weekly for 8 weeks and the diet increased accordingly. Survival was greater than 95% in all treatments, with no significant differences observed among treatments. There were also no differences in specific growth rate (SGR 2.12–2.27) of the fish among treatments. Growth rate was linear during the 8 weeks in all treatments, and harvested biomass increased proportionally to stocking density (198, 401, 600 and 785 g per increasing stocking density, respectively). Feed efficiency (FE 0.67–0.71) of the fish did not vary significantly among treatments. The coefficient of variation was high (35–41%) among the harvested fish, but it also did not differ significantly among treatments. The final condition indices of the fish in all treatments were similar to each other but significantly greater than the initial values (P < 0.05). The results suggest that there is no apparent effect of stocking density at the levels tested on the survival and growth of juvenile rabbitfish.  相似文献   

Regulation of mycorrhiza development in durum wheat by P fertilization: Effect on plant nitrogen metabolism          下载免费PDF全文

Catello Di Martino  Giuseppe Palumbo  Domenico Vitullo  Patrick Di Santo  Amodio Fuggi 《植物养料与土壤学杂志》2018,181(3):429-440

The aim of this work was to study the effect of arbuscular mycorrhizal fungus Glomus mosseae on growth and nitrogen (N) metabolism of durum wheat (Tritcum durum) under various P soil contents. The analyses were extended to macro and micronutrient tissue concentrations, nitrate reductase and glutamine synthetase activities, as well as protein, aminoacids, pyridine dinucleotides and adenine nucleotides. Arbuscular mycorrhiza increased wheat growth in soil in which P availability was low and nitrate was the dominant N form. The root colonization occurred at the highest level in plants grown in limiting soil P and was inversely related to soil P content. The micorrhizal wheat plants contained also the highest concentrations of macro (P, K, Ca, N) and micronutrients (Fe, Zn, Mn) as well as free amino acids, protein, NAD, NADP, AMP, ADP, ATP in roots and leaves. In particular, the micronutrient tissue concentrations (Zn, Mn) supported that mycorrhiza actively modulated their uptake limiting interferences and optimizing growth better than the plant roots, like a very efficient “rootstock”. Control plants grown at the highest soil P did not reach the same concentration as the mycorrhizal plants. Nitrate reductase activities in the roots of mycorrhizal plants were higher than in the control ones, while glutamine synthetase activities were highest in the leaves. Protein and amino acids concentrations, as well as AMP, ADP, ATP, NAD(P), and NAD(P)H were also higher than in the control. Among the free amino acids in the roots, the high levels of glutamine, asparagine, arginine, support the view that ammonium was transferred through the arbuscules to the root cells where it was re‐assimilated in the cortical cells, forming high N : C ratio‐amino acids. They were transferred to the leaves where all the other N compounds could be largely synthesized using the carbon skeletons supplied by photosynthesis.  相似文献   

Nutrient limitations to bacterial and fungal growth during cellulose decomposition in tropical forest soils     

Andrew?T.?NottinghamEmail authorView author&#;s OrcID profile  Lettice?C.?Hicks  Adan?J.?Q.?Ccahuana  Norma?Salinas  Erland?B??th  Patrick?Meir 《Biology and Fertility of Soils》2018,54(2):219-228

Nutrients constrain the soil carbon cycle in tropical forests, but we lack knowledge on how these constraints vary within the soil microbial community. Here, we used in situ fertilization in a montane tropical forest and in two lowland tropical forests on contrasting soil types to test the principal hypothesis that there are different nutrient constraints to different groups of microorganisms during the decomposition of cellulose. We also tested the hypotheses that decomposers shift from nitrogen to phosphorus constraints from montane to lowland forests, respectively, and are further constrained by potassium and sodium deficiency in the western Amazon. Cellulose and nutrients (nitrogen, phosphorus, potassium, sodium, and combined) were added to soils in situ, and microbial growth on cellulose (phospholipid fatty acids and ergosterol) and respiration were measured. Microbial growth on cellulose after single nutrient additions was highest following nitrogen addition for fungi, suggesting nitrogen as the primary limiting nutrient for cellulose decomposition. This was observed at all sites, with no clear shift in nutrient constraints to decomposition between lowland and montane sites. We also observed positive respiration and fungal growth responses to sodium and potassium addition at one of the lowland sites. However, when phosphorus was added, and especially when added in combination with other nutrients, bacterial growth was highest, suggesting that bacteria out-compete fungi for nitrogen where phosphorus is abundant. In summary, nitrogen constrains fungal growth and cellulose decomposition in both lowland and montane tropical forest soils, but additional nutrients may also be of critical importance in determining the balance between fungal and bacterial decomposition of cellulose.  相似文献   

Comparison of effects of high-pressure processing and heat treatment on immunoactivity of bovine milk immunoglobulin G in enriched soymilk under equivalent microbial inactivation levels     

Li SQ  Zhang HQ  Balasubramaniam VM  Lee YZ  Bomser JA  Schwartz SJ  Dunne CP 《Journal of agricultural and food chemistry》2006,54(3):739-746

Immunoglobulin-rich foods may provide health benefits to consumers. To extend the refrigerated shelf life of functional foods enriched with bovine immunoglobulin G (IgG), nonthermal alternatives such as high-pressure processing (HPP) may offer advantages to thermal processing for microbial reduction. To evaluate the effects of HPP on the immunoactivity of bovine IgG, a soymilk product enriched with milk protein concentrates, derived from dairy cows that were hyperimmunized with 26 human pathogens, was subjected to HPP or heat treatment. To achieve a 5 log reduction in inoculated Escherichia coli 8739, the HPP or heat treatment requirements were 345 MPa for 4 min at 30 degrees C or for 20 s at 70 degrees C, respectively. To achieve a 5 log reduction in natural flora in the enriched soymilk, the HPP or heat treatments needed were 552 MPa for 4 min at 30 degrees C or for 120 s at 78.2 degrees C, respectively. At equivalent levels for a 5 log reduction in E. coli, HPP and heat treatment caused 25% and no detectable loss in bovine IgG activity, respectively. However, at equivalent levels for a 5 log reduction in natural flora, HPP and heat resulted in 65 and 85% loss of bovine IgG activity, respectively. Results of combined pressure-thermal kinetic studies of bovine milk IgG activity were provided to determine the optimal process conditions to preserve product function.  相似文献