Antagonistic interaction between Echinostoma revolutum and Echinoparyphium recurvatum (Trematoda) in the definitive host     

V Barus  F Moravec  B Rysavy 《Folia parasitologica》1974,21(2):155-159

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Observations on the development of Rhabdochona phoxini Moravec, 1968 (Nematoda: Rhabdochonidae).     

F Moravec 《Folia parasitologica》1976,23(4):309-320

The life-cycle of the nematode Rhabdochona phoxini, an intestinal parasite of the fish Phoxinus phoxinus, has been studied experimentally. The mayfly nymphs, Habrophlebia lauta Eaton and H. fusca (Curtis) were found to serve as experimental intermediate hosts. After the eggs of R. phoxini have been swallowed by the mayfly nymph, the toothed first-stage larvae of this parasite are released and penetrate through the intestinal wall into t-e body cavity of the mayfly. Before reaching the infective third stage, the larvae moult twice in the body of the invertebrate host (2 to 16 and20 to 36 days after infection at water temperatures of 13--15 degrees C). However, the development of the encysted infective larvae does not cease in the intermediate host and the larvae may undergo one more moult. One of two moults then occur in the intestine of the definitive host (Phoxinus phoxinus) (depending on the fact whether it was infected with third- or fourth-stage larvae) before the larvae attain the maturity. The development of R. phoxini in the definitive host lasts about two months.  相似文献   

The modern state of the European saiga population (Saiga tatarica tatarica): mtDNA,DRB3 MHC gene,and microsatellite diversity     

Nadezhda Vladimirovna KASHININA  Anna Anatolievna LUSHCHEKINA  Pavel Alexandrovich SOROKIN  Karina Karenovna TARASYAN  Marina Vladimirovna KHOLODOVA 《Integrative zoology》2023,18(4):661-676

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Supercritical fluid extraction of bioavailable amino acids from soils and their liquid chromatographic determination with fluorometric detection     

Lojková L  Klejdus B  Formánek P  Kubán V 《Journal of agricultural and food chemistry》2006,54(17):6130-6138

A new procedure with supercritical CO2 modified with 0.5 mL of water and 0.75 mL of 0.1 M HCl in situ and 0.75 mL of water on-line at 15 MPa and 50 degrees C for 45 min was applied for the extraction of bioavailable amino acids from soil samples. Total extraction time was 60 min, but more favorable conditions are even possible for selected groups of amino acids. All analytes were trapped into 20 mL of methanol with satisfactory recovery (94-104%) and determined using high-performance liquid chromatography with fluorometric detection on a Zorbax Eclipse column (4.6 x 75 mm, 3.5 microm) with Na2HPO4 and acetonitrile/methanol/water as a mobile phase. Linear calibration curves were obtained (r > 0.999 except 0.99823 for Ile) with lower limits of detection (S/N = 3) in the range from 1.54 pg (Gly) to 13.5 pg (Cy2) or from 18.6 fmol (Ser) to 64.8 fmol (Lys). Validation and repeatability data are also given. Comparable results were obtained with a robust, commonly used extraction method (0.5 M ammonium acetate, 60 min in shaker, followed by filtration and lyophilization). Limiting values of artificial release of amino acids were also determined for each soil sample to eliminate any false results to ensure that all extracted amino acids originate from soil solution and exchangeable bound positions of soil samples.  相似文献   

Systems science for policy evaluation     

Kabat P 《Science (New York, N.Y.)》2012,336(6087):1398

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Effect of medetomidine-butorphanol and dexmedetomidine-butorphanol combinations on intraocular pressure in healthy dogs     

Rauser P  Pfeifr J  Proks P  Stehlík L 《Veterinary anaesthesia and analgesia》2012,39(3):301-305

ObjectiveTo assess the effects of intravenous (IV) medetomidine-butorphanol and IV dexmedetomidine-butorphanol on intraocular pressure (IOP).Study designProspective, randomized, blinded clinical study.AnimalsForty healthy dogs. Mean ± SD body mass 37.6 ± 6.6 kg and age 1.9 ± 1.3 years.MethodsDogs were allocated randomly to receive an IV combination of dexmedetomidine, 0.3 mg m^?2, combined with butorphanol, 6 mg m^?2, (group DEX) or medetomidine 0.3 mg m^?2, combined with butorphanol 6 mg m^?2, (group MED). IOP and pulse (PR) and respiratory (f_R) rates were measured prior to (baseline) and at 10 (T10), 20 (T20), 30 (T30) and 40 (T40) minutes after drug administration. Oxygen saturation of hemoglobin (SpO₂) was monitored following sedation. Data were analyzed by anova followed by Dunnett's tests for multiple comparisons. Changes were considered significant when p < 0.05.ResultsFollowing drug administration, PR and f_R were decreased significantly at all time points but did not differ significantly between groups. Baseline IOP in mmHg was 14 ± 2 for DEX and 13 ± 2 for MED. With both treatments, at T10, IOP increased significantly (p < 0.001), reaching 20 ± 3 and 17 ± 2 for DEX and MED respectively. This value for DEX was significantly higher than for MED. There were no significant differences in IOP values between groups at any other time points. At T30 and T40, IOP in both groups was below baseline (DEX, 12 ± 2 and 11 ± 2: MED 12 ± 2 and 11 ± 2) and this was statistically significant, for DEX.Conclusions and clinical relevanceAt the documented doses, both sedative combinations induced a transient increase and subsequent decrease of IOP relative to baseline, which must be taken into consideration when planning sedation of animals in which marked changes in IOP would be undesirable.  相似文献   

家禽精液冷冻保存技术研究进展     

邓溯顺  燕海峰  易康乐  Pavel Trefil  陈铁桥 《山东家禽》2012,(5):45-48

家鸡精液冷冻保护是物种资源保存最廉价的方法。本文从家鸡精液的稀释液、冷冻保护剂、冻精形式、平衡时间、冷冻速率、解冻速率、精液质量检测方法等几个方面,综述了国内外研究进展,并对目前家鸡精液冷冻技术应用存在的问题进行了分析,提出了初步研究思路。  相似文献   

Incidence of seizures associated with iopamidol or iomeprol myelography in dogs with intervertebral disk disease: 161 cases (2000–2002)     

Leona Lexmaulova MVDr  Josef Zatloukal MVDr  PhD  Pavel Proks MVDr  Milan Dvorak MVDr  PhD  Robert Srnec MVDr  Petr Rauser MVDr  PhD  Helga Kecova MVDr  PhD  Alois Necas MVDr  PhD  MBA 《Journal of Veterinary Emergency and Critical Care》2009,19(6):611-616

Objective – To compare the incidence of seizures in dogs with intervertebral disk disease after iopamidol or iomeprol myelography, and to assess whether the incidence of seizures differed between the 2 agents when severity of neurological deficits, location of cord compression, duration of anesthesia, site of myelogram, volume of contrast, and concentration of contrast were evaluated. Design – Retrospective study. Setting – Veterinary teaching hospital. Animals – One hundred and sixty‐one client‐owned dogs with intervertebral disk disease. Interventions – Subarachnoid injection of contrast medium. Measurements and Main Results – One hundred and sixty‐one dogs with intervertebral disk disease were subjected to myelography using iopamidol (n=74) or iomeprol (n=87). Cranial myelography was performed in 31 dogs, caudal myelography in 125 and both cranial and caudal myelography in 5. Seizures occurred in 23 of 161 (14%) dogs. There was no significant difference overall between iopamidol and iomeprol myelography. However, in dogs with thoracolumbar disk extrusion and paraplegia, seizures occurred more frequently after caudal myelography using iopamidol compared with iomeprol. Conclusions – Both iomeprol and iopamidol are suitable for myelography in dogs. Iomeprol is recommended for caudal myelography in paraplegic dogs with thoracolumbar disk extrusion due to the higher incidence of seizures in this group when iopamidol was used.  相似文献   

Production of UV-light-detectable faeces from house mice (Mus musculus domesticus) after consumption of encapsulated fluorescent pigment in monitoring bait     

Frynta D  Eliášová B  Fraňková M  Aulický R  Rödl P  Stejskal V 《Pest management science》2012,68(3):355-361

BACKGROUND: The authors investigated whether fluorescent pigment in thermoset melamine microcapsules incorporated into monitoring baits would be excreted in the faeces of wild house mice in a quantity and intensity that would be detectable by a human observer. RESULTS: Experimental mice produced 24–116 UV‐visible faecal pellets per 24 h; the mean dry weight was 582 mg. The number and weight of the faeces was independent of mouse sex and weight. The defecation of UV‐visible faeces began at 2–3 h, peaked at 5–8 h and was complete at 17 h after bait ingestion. The detectability of the highly fluorescent faecal pellets using a small UV flashlight approached 100%, and no false positives were recorded. CONCLUSION: The tested formulation is of significant value for rodent pest monitoring because faeces that are highly visible by UV light are produced for 15 h by mice after ingestion, and their detection is easy and unambiguous. Copyright © 2011 Society of Chemical Industry  相似文献   

Nematodes parasitizing domestic ruminants in Afghanistan.     

V Barus  A Amin  K Blazek  F Moravec 《Folia parasitologica》1976,23(3):207-216

A total of 12 nematode species were determined in the collection recovered from domestic ruminants in Afghanistan. Ten of them were found for the first time in this region: Trichocephalus globulosus, Trichostrongylus probolurus, Cooperia bisonis, Nematodirus spathiger, N. abnormalis, Thelazia rhodesi, Bunostomum phlebotomum, Haemonchus contortus, Dictyocaulus filaria and Oesophagostomum radiatum. Morphological and metrical characteristics of T. globulosus, T. skrjabini and C. bisonis, based on our own material, are added.  相似文献