Ages of prehistoric earthquakes revealed by cosmogenic chlorine-36 in a bedrock fault scarp at hebgen lake   总被引：3，自引：0，他引：3  

M Zreda  JS Noller 《Science (New York, N.Y.)》1998,282(5391):1097-1099

Cosmogenic chlorine-36 reveals dates of the multiple prehistoric earthquakes that have produced a scarp on the Hebgen Lake fault. Apparent chlorine-36 ages are stratigraphically correct, follow a predicted theoretical pattern, and produce geologically reasonable model ages of 24, 20, 7.0, 2.6, 1.7, and 0.4 thousand years ago. This result demonstrates the feasibility of using cosmogenic chlorine-36 to extract paleoearthquake records from bedrock fault scarps.  相似文献   

Role of Embryonic and Maternal Genotype on Prenatal Survival and Foetal Growth in Rabbit          下载免费PDF全文

C Naturil‐Alfonso  F Marco‐Jiménez  E Jiménez‐Trigos  MD Saenz‐de‐Juano  MP Viudes‐de‐Castro  R Lavara  JS Vicente 《Reproduction in domestic animals》2015,50(2):312-320

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Effect of Different Media and Protein Source on Equine Gametes: Potential Impact During In Vitro Fertilization          下载免费PDF全文

L González‐Fernández  S Macedo  JS Lopes  A Rocha  B Macías‐García 《Reproduction in domestic animals》2015,50(6):1039-1046

Equine in vitro fertilization (IVF) is still inconsistent. In the present work, we studied how modified Whitten's (MW) medium and Tissue Culture Medium 199 (TCM) added with Foetal Bovine Serum (FBS; 10% v/v) or Bovine Serum Albumin (BSA; 7 mg/ml) affected equine gametes to subsequently run IVF trials. Compact (Cp) and expanded (Ex) cumuli equine oocytes were matured and placed in TCM or MW supplemented with BSA or FBS for 18–20 h (no sperm added). In Ex oocytes, TCM‐199 added with FBS or BSA resulted in higher metaphase II (MII) rates (75.7% and 62.7%, respectively) than MW added with BSA (54%) or FBS (52.2%; p < 0.05); this was not observed for Cp oocytes. Equine sperm were capacitated in the same media at 10 × 10⁶ sperm/ml for 4 h at 37°C; total motility and protein tyrosine phosphorylation (PY) were evaluated. While motility remained unchanged, TCM or MW added with FBS enhanced the number of sperm showing PY‐stained tails (25 ± 4.8% and 31 ± 6.6%; mean ± SEM, respectively) over BSA supplemented media (3 ± 1.2% and 11.7 ± 1.1%) for TCM and MW (p < 0.05). In view of the previous results, sperm were capacitated in TCM + FBS and MW + BSA (control); IVF trials were run in the same media supplemented with 200 ng/ml of progesterone, but no fertilization occurred. Our results show that TCM + FBS enhances Ex equine oocyte's meiotic competence over MW + BSA and TCM or MW added with FBS successfully induce equine PY over media supplemented with BSA.  相似文献   

Black-hair follicular dysplasia in a New Zealand Huntaway dog     

JS Munday  AF French  GRK McKerchar 《New Zealand veterinary journal》2013,61(3):170-172

CASE HISTORY: A 6-year-old intact male New Zealand Huntaway dog had slowly progressive alopecia that was first observed at 12 weeks of age.

CLINICAL FINDINGS: Patchy alopecia was confined to the black-haired areas of the body, and was most evident on the head and dorsum of the body; tan-haired areas of skin appeared normal. Histological examination of black-haired skin revealed distended melanocytes and large aggregates of melanin within, and surrounding, the hair follicles and the epidermis. Macrophages distended with melanin were also visible within the perifollicular and superficial dermis, and follicular lumina were often plugged by keratin that contained aggregates of melanin. The follicles were dysplastic and few hair shafts were visible emerging from follicular infundibula within the sections.

DIAGNOSIS: The clinical and histological findings were consistent with black-hair follicular dysplasia (BHFD).

CLINICAL RELEVANCE: This is the first report of BHFD in a dog in New Zealand, and is the first report in a Huntaway. The most significant effect of BHFD is a predisposition to follicular plugging and secondary bacterial skin infections. Due to the hereditary nature of the follicular dysplasias, breeding from affected dogs should be discouraged. Histological examination of the skin is required to differentiate between the different follicular dysplasias as well as differentiating between follicular dysplasia and follicular atrophy due to endocrinopathy.  相似文献   

Confirmation of the occurrence of the nematode parasite Lamanema chavezi Becklund, 1963 in South American camelids in New Zealand     

PB McKenna  C Morley  M Koning  JS Tahana  MJ Taylor 《New Zealand veterinary journal》2013,61(6):395-396

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Disseminated Mortierella wolfii infection in a neonatal calf     

JS Munday  AG Wolfe  KE Lawrence  SK Pandey 《New Zealand veterinary journal》2013,61(1):62-63

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Competitive exclusion of Yersinia enterocolitica biotype 4, serotype O:3 by Yersinia enterocolitica biotype 1A,serotype O:6,30 in tissue culture and in pigs     

HM Hussein  SG Fenwick  JS Lumsden 《New Zealand veterinary journal》2013,61(5):227-231

AIMS: To study the adhesion properties of a biotype 4, serotype O:3 (human pathogenic) strain of Yersinia enterocolitica and to determine if adhesion in vitro and colonisation in vivo can be prevented by competition with a biotype 1A, serotype O:6,30 (non-pathogenic) strain. To study interaction between Y. enterocolitica biotype 4, serotype O:3 and cultured epithelial cells using the synthetic tripeptide arginine-glycine-aspartic acid (RGD).

METHODS: The human intestinal epithelial (HEp-2) cell line was used for in vitro studies. Inocula of Y. enterocolitica biotype 4, serotype O:3 radiolabelled using tritium were incubated with HEp-2 cells and RGD tripeptide, or with Y. enterocolitica biotype 1A, serotype O:6,30 sequentially or concurrently, then washed and lysed, and radioactivity measured to determine the effect of RGD on adhesion, and competitive exclusion of pathogenic by non-pathogenic bacteria. For in vivo studies, two groups of 5-week-old piglets (n=5/group) were sequentially inoculated orally with 5x10⁹ colony forming units (cfu) of either a non-pathogenic biotype 1A, serotype O:6,30 strain of Y. enterocolitica followed by a pathogenic biotype 4, serotype O:3 strain, or vice versa. Pigs were monitored for carriage of strains using bacterial culture and a multiplex polymerase chain reaction (PCR).

RESULTS: The RGD tripeptide significantly inhibited adherence of the pathogenic Y. enterocolitica strain to cultured epithelial cells, suggesting that adhesion involved the RGD tripeptide sequence. The non-pathogenic biotype 1A, serotype O:6,30 strain of Y. enterocolitica prevented adhesion of the pathogenic strain to cells in vitro when allowed to adhere first. Pathogenic Y. enterocolitica was consistently isolated from rectal swabs from 80-100% of pigs on all sampling occasions but not from oral swabs after 14 days in pigs first inoculated with the non- pathogenic strain or at 26 days in pigs first inoculated with the pathogenic strain.

CONCLUSIONS: A non-pathogenic strain of Y. enterocolitica reduced adhesion of a human pathogenic strain in vitro but not in vivo.  相似文献   

A real-time polymerase chain reaction assay for the detection of Mycoplasma agalactiae     

J Fitzmaurice  M Sewell  CM King  S McDougall  WL McDonald  JS O'Keefe 《New Zealand veterinary journal》2013,61(5):233-236

AIM: To develop a real-time PCR for the detection of Mycoplasma agalactiae, using PCR primers targeting the ma-mp81 gene.

METHODS: A group of 15 M. agalactiae isolates, 21 other Mycoplasma spp. isolates and 21 other bacterial isolates was used in evaluation of the assay.

RESULTS: All M. agalactiae isolates were detected by the assay and none of the non-target isolates was amplified. The analytical detection limit of the assay was 10 fg of purified genomic DNA and 10⁴ cfu/ml milk inoculated with M. agalactiae. When applied to goat-milk samples collected from three herds free of M. agalactiae infection, the assay had a specificity of 100%.

CONCLUSIONS: The assay would be useful in a diagnostic laboratory, providing specific, sensitive and rapid detection of M. agalactiae.  相似文献   

Vaccination-site sarcomas in cats in New Zealand     

D Aberdein  JS Munday  CB Dyer  CG Knight  AF French  IR Gibson 《New Zealand veterinary journal》2013,61(1)

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Maternal Exposure to High Temperatures Disrupts OCT4 mRNA Expression of Rabbit Pre‐Implantation Embryos and Endometrial Tissue     

F Marco‐Jiménez  C Naturil‐Alfonso  DS Peñaranda  E Jiménez‐Trigos  FJ García‐Diego  JS Vicente 《Reproduction in domestic animals》2013,48(3):429-434

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