Effects of formic acid and potassium diformate on the fermentation quality,chemical composition and aerobic stability of alfalfa silage          下载免费PDF全文

X. Yuan  A. Wen  Z. Dong  S. T. Desta  T. Shao 《Grass and Forage Science》2017,72(4):833-839

The objective of this study was to evaluate the efficacy of potassium diformate (KDF) as a potential additive for alfalfa silage. Fresh alfalfa was untreated or treated with formic acid (4 g/kg fresh weight, FW) or three concentrations of KDF (4, 5.5 or 7 g/kg FW). After 60 days of ensiling, the addition of formic acid and greater levels of KDF (5.5 and 7 g/kg) effectively reduced silage pH and inhibited the undesirable bacteria, indicated by lower butyric acid, ethanol, ammonia N concentrations and microbial populations (including enterobacteria, yeasts, moulds and clostridia). Additives decreased the dry‐matter loss, and more water‐soluble carbohydrates were preserved in the silages with formic acid or potassium diformate than in the control. Alfalfa silages treated with formic acid at 4 g/kg FW or potassium diformate at 5.5 or 7 g/kg FW were classified as the highest quality silage based on the higher Flieg's point (above 70) and remained stable for more than 9 days during aerobic exposure. Potassium diformate is recommended as an effective additive for alfalfa silages at a level of 5.5 or 7 g/kg FW under the humid and hot conditions of southern China.  相似文献   

Temporal and spatial progress of the diseases caused by the crinivirus tomato chlorosis virus and the begomovirus tomato severe rugose virus in tomatoes in Brazil     

M. A. Macedo  A. K. Inoue-Nagata  T. N. Z. Silva  D. M. S. Freitas  J. A. M. Rezende  J. C. Barbosa  M. Michereff-Filho  A. R. Nascimento  A. L. Lourenção  A. Bergamin Filho 《Plant pathology》2019,68(1):72-84

Efficient management of whitefly-borne diseases remains a challenge due to the lack of a comprehensive understanding of their epidemiology, particularly of the diseases tomato golden mosaic and tomato yellowing. Here, by monitoring 16 plots in four commercial fields, the temporal and spatial distribution of these two diseases were studied in tomato fields in Brazil. In the experimental plots these diseases were caused by tomato severe rugose virus (ToSRV) and tomato chlorosis virus (ToCV), respectively. The incidence of each virus was similar in the plots within a field but varied greatly among fields. Plants with symptoms for both diseases were randomly distributed in three of four spatial analyses. The curves representing the progress of both diseases were similar and contained small fluctuations, indicating that the spread of both viruses was similar under field conditions. In transmission experiments of ToSRV and ToCV by Bemisia tabaci MEAM1 (former biotype B), these viruses had a similar transmission rate in single or mixed infections. It was then shown that primary and secondary spread of ToCV were not efficiently controlled by insecticide applications. Finally, in a typical monomolecular model of disease progress, simulation of the primary dissemination of ToSRV and ToCV showed that infected plants were predominantly randomly distributed. It is concluded that, although the manner of vector transmission differs between ToSRV (persistent) and ToCV (semipersistent), the main dispersal mechanisms are most probably similar for these two diseases: primary spread is the predominant mechanism, and epidemics of these diseases have been caused by several influxes of viruliferous whiteflies.  相似文献   

Genetic diversity of the pine pathogen Lecanosticta acicola in Slovenia and Croatia     

D. Sadiković  B. Piškur  I. Barnes  T. Hauptman  D. Diminić  M. J. Wingfield  D. Jurc 《Plant pathology》2019,68(6):1120-1131

Brown spot needle blight (BSNB), a disease of pine trees caused by the fungus Lecanosticta acicola, has been known in Slovenia since 2008 and in Croatia since 1975. Recent outbreaks in Slovenia prompted this study to compare L. acicola populations in these two neighbouring European countries. Sixty-nine isolates collected from three pine species (Pinus mugo, P. halepensis and P. nigra) were used to determine the phylogenetic relationships, genetic structure, and reproductive strategy of the pathogen. EF1-α sequences showed that Slovenian and Croatian isolates share a common ancestry with individuals from central and northern Europe. Population structure analysis revealed four distinct population clusters of L. acicola in these two countries, generally corresponding to their respective geographic location and host. An unequal ratio of mating types and a low overall genetic diversity in the population indicated a strong influence of asexual reproduction. Although some of the oldest recorded European occurrences of BSNB are from Croatia, this study provided no evidence that the population studied in Croatia was the source of the sampled outbreaks in Slovenia. Recent outbreaks of L. acicola in Slovenia are most likely due to introductions from other, yet to be identified, sources.  相似文献   

Exogenous application of melatonin improves plant resistance to virus infection     

L. Zhao  L. Chen  P. Gu  X. Zhan  Y. Zhang  C. Hou  Z. Wu  Y.-F. Wu  Q.-C. Wang 《Plant pathology》2019,68(7):1287-1295

In this study, melatonin (MEL)-mediated plant resistance to tobacco mosaic virus (TMV) was examined to study local infection in Nicotiana glutinosa and systemic infection in Solanum lycopersicum. Exogenous application of 100 µm MEL increased anti-virus infection activity to 37.4% in virus-infected N. glutinosa plants. The same treatment significantly reduced relative levels of virus RNA analysed by qRT-PCR and virus titres measured by dot-ELISA, and increased the relative expression levels of the PR1 and PR5 genes analysed by qRT-PCR, in virus-infected S. lycopersicum. MEL treatment induced considerable accumulations of salicylic acid (SA) and nitric oxide (NO) but did not significantly affect production of hydrogen peroxide (H₂O₂) in the virus-infected S. lycopersicum plants. Transgenic nahG N. tabacum was used to determine whether MEL-induced TMV resistance was dependent on the SA pathway. The results showed that the relative RNA level of the TMV analysed by qRT-PCR and virus titres analysed by dot-ELISA were not reduced by the MEL treatment in the nahG transgenic N. tabacum seedlings treated twice with 100 µm MEL. The increased relative expression levels of PR1 and PR5 were greatly reduced when cPTIO, an NO scavenger, was included in the MEL treatment. A working model of MEL-mediated plant resistance to TMV is proposed. MEL-mediated plant resistance to viruses provides a new avenue to control plant viral diseases.  相似文献   

Proteomic analysis of the mango anthracnose pathogen Colletotrichum gloeosporioides treated with borate highlights distinct mitochondrial response mechanisms     

S. T. He  T. T. Chen  X. B. Xu  Z. K. Zhang  H. C. Song  H. M. Song  L. H. Meng  P. Zhou  X. Q. Shi 《Plant pathology》2019,68(7):1369-1380

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Distribution and genetic diversity among Aspergillus flavus isolates across three agro-ecologies essential for maize cultivation in Ghana     

M. A. Dadzie  A. Oppong  K. Ofori  J. S. Y. Eleblu  B. E. Ifie  E. T. Blay  E. Obeng-Bio  Z. Appiah-Kubi  M. L. Warburton 《Plant pathology》2019,68(8):1565-1576

Fungal mycotoxins are important contaminants of agricultural commodities that pose serious concerns to producers, consumers and exporters. Aflatoxin is a carcinogenic metabolite produced by the fungi Aspergillus flavus and A. parasiticus. These fungi and other mould species living in the soil contaminate several crops including maize. This study was undertaken with the following objectives: (i) to assess the presence of A. flavus and other moulds in Ghanaian soil; (ii) to determine the distribution and density of A. flavus under three agro-ecologies noted for major maize production; and (iii) to assess the effect of percentage of carbon (C) and nitrogen (N) and soil pH on A. flavus population densities and diversity in the isolates found across the agro-ecologies. The data showed seven moulds that were common across the agro-ecologies. Significant differences (P < 0.05) were observed in the A. flavus density and distribution within and across these agro-ecologies. Fumesua soils recorded the highest levels of A. flavus (1.185 × 10³ cfu g⁻¹) while Akomadan recorded the least (9.76 × 10² cfu g⁻¹). Percentage available C, N and soil pH did not significantly influence A. flavus density. The A. flavus isolates identified in this study varied in genetic sequence within the aflatoxin gene cluster, but these differences were not distinguishable by origin.  相似文献   

Comparative performance of the highly invasive Mimosa invisa and its less invasive subspecies under different nutrient and light conditions     

Y Wang  Z W Fan  Y D Shen  X X Li  Y Liu  Q Q Huang 《Weed Research》2019,59(6):419-426

Invasive plants may be more plastic than non‐invasive plants and maintain high fitness under various environmental conditions. Previous studies mainly focused on the comparisons between invasive and native plants, and comparisons between highly invasive and less invasive exotic species are still relatively rare, especially for comparisons at the subspecies level. This study examined the effects of nutrient addition and shading on the performance of the highly invasive Mimosa invisa and its less invasive subspecies M. invisa var. inermis under either isolated or competitive conditions. Nutrient addition increased biomass and plant height and decreased root‐to‐shoot ratio (R/S). Shading decreased biomass and R/S and increased plant height. Under isolated conditions, the two invaders did not differ in R/S, plant height and plasticity of these traits in response to nutrient addition or shading, and the two invaders also did not differ in biomass production under each of the nutrient and light treatments. When the two invaders competed with each other, M. invisa outcompeted M. invisa var. inermis under high soil nutrient conditions, and the two invaders did not differ in performance under other growth conditions. Thus, only considering competition may we find out the difference between highly invasive species and their closely related, less invasive subspecies. Management of M. invisa should focus on habitats with high soil nutrient availability, in which M. invisa is more likely to dominate.  相似文献   

Testing the ability of unmanned aerial systems and machine learning to map weeds at subfield scales: a test with the weed Alopecurus myosuroides (Huds)     

James PT Lambert  Dylan Z Childs  Rob P Freckleton 《Pest management science》2019,75(8):2283-2294

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加工番茄果秧分离装置运动过程分析   总被引：4，自引：0，他引：4  

李成松  坎杂  谭洪洋  孙日宾  梁荣庆  冯玉磊 《农业机械学报》2012,43(4):66-69

为了深入研究加工番茄果秧分离特性,分析了加工番茄果秧分离装置激振器的运动过程,利用达朗贝尔原理建立其理论模型。利用SolidWorks对激振器的运动过程进行了仿真,并在FS-35型加工番茄果秧分离试验台上,利用高速摄像机拍摄了分离滚筒的运动过程,利用Blaster’s Mas软件对录像进行了处理和分析。分析结果表明,理论模型与实际运动吻合较好,验证了理论模型的正确性。  相似文献   

基于关节力传感器的并联六自由度机构标定方法     

皮阳军  王宣银  胡玉梅 《农业机械学报》2012,43(10):215-218

提出了一种基于关节力传感器的并联六自由度机构结构参数标定方法.从力学角度出发,通过测量并联六自由度机构各关节驱动力,利用并联六自由度机构自身的运动学和动力学模型构造相应的辨识模型,实现其结构参数的标定.通过标定仿真验证了该方法的实用性和有效性.  相似文献