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961.
In marine ecology, population abundance time series often reveal patterns, such as decadal pseudo-cycles, on scales that make them difficult to mathematically characterize by means of conventional models based on functional responses. We propose here a simple non-deterministic model based on three strong ecological constraints, without any use of functional responses or external factors: (i) mass balance constraints between flows and biomass, (ii) a satiation constraint relating inflows and biomass and (iii) an inertia principle restricting the variation of populations of a given species according to its lifespan. This model reproduces in a robust manner observed patterns of variability and can be used to question the relevance of other modelling approaches of ecosystem dynamics with regard to determinism, constraints and stochasticity. Referring to a non-deterministic model without any functional relationships and environmental or anthropogenic forcing can help in avoiding misleading advice based on the belief that we can explain the causes of observed patterns, which may simply result from basic structural constraints within which the ecosystem functions.  相似文献   
962.
In 1935, Bienenstock and coworkers claimed the presence of gluten-like material in the germ of the carob seed. The viscoelastic properties of the water-insoluble proteins isolated from carob germ, which we propose to call caroubin, have been confirmed by dynamic (G′ and G″) and static rheological measurements (texture profile analysis, viscoelastogram). Biochemical analyses showed important similarities (high glutamic acid content, size-exclusion HPLC profile, PAGE patterns of reduced and unreduced proteins) as well as large differences (high arginine and low cysteine and proline content of caroubin, carbohydrate composition) between caroubin and wheat gluten. Besides potential new industrial uses of carob seed, caroubin could be a valuable material to help us understand the physicochemical basis of the viscoelastic properties of plant protein complexes like wheat gluten.  相似文献   
963.
When mycelium of Botrytis cinerea was treated with low concentrations of the anilinopyrimidine fungicide pyrimethanil the total amount of free amino acids increased. Qualitative variations were also induced: alanine, glutamine, lysine, glycine, histidine, asparagine, arginine, threonine and moreover, α-aminobutyrate and β-alanine were accumulated; cyst(e)ine, valine, leucine and citrulline were reduced. When mycelium of B. cinerea was incubated with Na2[35S]O4, pyrimethanil at 1·5 μM induced a decrease of [35S]methionine and simultaneously an increase of [35S]cystathionine. These data indicate that the anilinopyrimidine fungicide pyrimethanil inhibits the biosynthesis of methionine and suggest that the primary target could be the cystathionine β-lyase. © 1997 SCI.  相似文献   
964.
Since the beginning of the 21st century, electronic monitoring (EM) has emerged as a cost‐efficient supplement to existing catch monitoring programmes in fisheries. An EM system consists of various activity sensors and cameras positioned on vessels to remotely record fishing activity and catches. The first objective of this review was to describe the state of play of EM in fisheries worldwide and to present the insights gained on this technology based on 100 EM trials and 12 fully implemented programmes. Despite its advantages, and its global use for monitoring, progresses in implementation in some important fishing regions are slow. Within this context, the second objective was to discuss more specifically the European experiences gained through 16 trials. Findings show that the three major benefits of EM were as follows: (a) cost‐efficiency, (b) the potential to provide more representative coverage of the fleet than any observer programme and (c) the enhanced registration of fishing activity and location. Electronic monitoring can incentivize better compliance and discard reduction, but the fishing managers and industry are often reluctant to its uptake. Improved understanding of the fisher's concerns, for example intrusion of privacy, liability and costs, and better exploration of EM benefits, for example increased traceability, sustainability claims and market access, may enhance implementation on a larger scale. In conclusion, EM as a monitoring tool embodies various solid strengths that are not diminished by its weaknesses. Electronic monitoring has the opportunity to be a powerful tool in the future monitoring of fisheries, particularly when integrated within existing monitoring programmes.  相似文献   
965.
The production of piscine viruses, in particular of koi herpesvirus (KHV, CyHV‐3) and infectious salmon anaemia virus (ISAV), is still challenging due to the limited susceptibility of available cell lines to these viruses. A number of cell lines from different fish species were compared to standard diagnostic cell lines for KHV and ISAV regarding their capability to exhibit a cytopathic effect (CPE) and to accumulate virus. Two cell lines, so far undescribed, appeared to be useful for diagnostic purposes. Fr994, a cell line derived from ovaries of rainbow trout (Oncorhynchus mykiss), produced constantly high ISA virus (ISAV) titres and developed a pronounced CPE even at high cell passage numbers, while standard cell lines are reported to gradually loose these properties upon propagation. Another cell line isolated from the head kidney of common carp (Cyprinus carpio), KoK, showed a KHV induced CPE earlier than the standard cell line used for diagnostics. A third cell line, named Fin‐4, established from the fin epithelium of rainbow trout did not promote efficient replication of tested viruses, but showed antigen sampling properties and might be useful as an in vitro model for virus uptake or phagocytosis.  相似文献   
966.
967.
The Eastern Canadian northern shrimp population, representing one of the most important fisheries in the region, decreased dramatically since the mid‐2000s to a historical low in 2017, but changes were not spatially uniform. Applying a biophysical model within Newfoundland and Labrador (NL) management areas, we investigated connectivity processes during the long pelagic larval phase (2–3 months) of Pandalus borealis and key drivers of larval dispersal in different environmental conditions. We selected 3 years representative of contrasting North Atlantic Oscillation (NAO) phases to assess potential larval dispersal patterns of the northern shrimp population in NL, and hierarchically assessed the impact of the timing of release (yearly and daily), release location, and vertical migration behaviour on shrimp larval dispersal. Overall, we found that populations located on the northern Newfoundland and Labrador shelf supplied potential settlers to southern populations because of the dominant Labrador Current. Ocean circulation and current velocities during the NAO positive year differed from other years, generating contrasting settlement spatial patterns. Larval release location and vertical migration behaviour were the two most important influences on the strength of larval supply and settlement patterns. Inclusion of diel and ontogenic swimming behaviour increased settlement success of larvae released from inshore areas, regardless of study years. Our study improves understanding of northern shrimp stock‐recruitment relationships, their sensitivity to changing environmental conditions, and spatially non‐homogeneous population decline for bentho‐pelagic species with a long larval phase, which could potentially help improve management strategies.  相似文献   
968.
Due to the presence of an asymmetrically substituted C atom, dimethenamid [2-chloro-N-(2,4-dimethyl-3-thienyl)-N-(2-methoxy-1-methylethyl)acetamide], a recently introduced N-thienyl chloroacetamide herbicide, exists as two stereoisomers (S and R) having differing herbicidal activities as demonstrated with a selection of weeds and Lemna minor. The activity of the two isomers was investigated in greater detail with the green alga Scenedesmus acutus and compared to that of alachlor [2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide]. As with alachlor, the S isomer (5 μM ) strongly inhibited algal growth and fatty acid desaturation while the R isomer had no effect. In short-term experiments (up to 5·5 h), the S isomer and alachlor (100 μM ) inhibited [14C]acetate uptake and its incorporation into fatty acids in the same manner, while the R isomer did not. Incorporation of [14C]acetate into a non-lipid fraction of the algae was strongly inhibited by alachlor and the S isomer (100 μM ) and only slightly by the R isomer. A 50% inhibition of incorporation of [14C]oleic acid into the same non-lipid fraction was attained with less than 10-7 M of the S isomer while 10-5 M of the R form of dimethenamid achieved only a 40% inhibition. The same stereospecificity of the compound on growth, fatty acid desaturation, acetate uptake and oleic acid incorporation provides strong evidence that dimethenamid may act upon a primary, specific target in lipid metabolism. Furthermore, the comparable biological activities of dimethenamid and alachlor indicate that this target is common to both N-phenyl and N-thienyl chloroacetamide herbicides. © 1997 SCI.  相似文献   
969.
Published radiotherapy results for spinal nephroblastomas in dogs are limited. In this retrospective longitudinal study (1/2007–1/2022), five dogs with a median age of 2.8 years received post-operative 3D conformal, conventional fractionated radiotherapy (CFRT) with 2–4 fields (parallel-opposed with or without two hinge-angle fields), for an incompletely resected nephroblastoma. Clinical findings prior to surgery included one or more of the following: pelvic limb paresis (5), faecal incontinence (2), flaccid tail (1), non-ambulatory (2) and deep pain loss (1). All masses were located between T11 and L3 and surgically removed via hemilaminectomy. Dogs received 45–50 Gray (Gy) in 18–20 fractions, and no dogs received chemotherapy post-radiation. At analysis, all dogs were deceased, with none lost to follow-up. The median overall survival (OS) from first treatment to death of any cause was 3.4 years (1234 days; 95% CI 68 days-upper limit not reached; range: 68–3607 days). The median planning target volume was 51.3 cc, with a median PTV dose of 51.4 Gy and median D98 = 48.3 Gy. Late complications or recurrence was difficult to fully determine in this small dataset; however, some degree of ataxia persisted throughout life in all dogs. This study provides preliminary evidence that post-operative radiotherapy may result in prolonged survival times dogs with spinal nephroblastomas.  相似文献   
970.
Common wheat adulteration of durum wheat pasta was quantified using real‐time duplex polymerase chain reaction (PCR). The total DNA content of pasta was determined by amplifying part of a wheat gene encoding a lipid transfer protein, and common wheat DNA was quantified by amplifying part of the puroindoline‐b gene. Under the conditions defined by this study, for pasta with a theoretical adulteration of 3%, the experimentally determined mean value was 2.6–3.4%, depending on drying temperature. Pure durum wheat pastas were distinguished from adulterated pastas without ambiguity. This study demonstrates the feasibility of using real‐time duplex PCR to quantify common wheat adulteration of pasta dried at high temperature, quantification that was impossible with the French official peroxidase‐marker method.  相似文献   
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