Influence of category-heifers,primiparous and multiparous lactating cows-in a large-scale resynchronization fixed-time artificial insemination program     

Márcio de Oliveira Marques  Fábio Morotti  Camila Bizarro da Silva  Mario Ribeiro Júnior  Rubens César Pinto da Silva  Pietro Sampaio Baruselli  Marcelo Marcondes Seneda 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(3):367-371

This study was conducted to evaluate the influence of category (heifers, primiparous or multiparous cows) on pregnancy rates in a large scale resynchronization ovulation program. Nelore heifers (n = 903), primiparous lactating cows (n = 338) and multiparous lactating cows (n = 1,223) were synchronized using a conventional protocol of estradiol/P4-based fixed-time artificial insemination (FTAI). Thirty days after ultrasonography, females who failed the first FTAI were resynchronized with the same hormonal protocol prior to a second FTAI. The pregnancy status of each cohort was evaluated by ultrasonography 30 days after each FTAI. The average conception rate after the first FTAI and resynchronization was 80.5%. Heifers had a higher conception rate (85%) than primiparous (76%) or multiparous cows (78%; p = 0.0001). The conception rate after the first FTAI was similar among heifers (57%), primiparous cows (51%) and multiparous cows (56%; p = 0.193). After the second FTAI, heifers exhibited a higher conception rate (66%) than primiparous or multiparous cows (51%; p = 0.0001). These results demonstrate the feasibility of resynchronization in large beef herds for providing consistent pregnancy rates in a short period of time. We also demonstrated that ovulation resynchronization 30 days after FTAI is particularly effective for heifers, providing a conception rate of up to 66%.  相似文献   

The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specialization     

Cuomo CA  Güldener U  Xu JR  Trail F  Turgeon BG  Di Pietro A  Walton JD  Ma LJ  Baker SE  Rep M  Adam G  Antoniw J  Baldwin T  Calvo S  Chang YL  Decaprio D  Gale LR  Gnerre S  Goswami RS  Hammond-Kosack K  Harris LJ  Hilburn K  Kennell JC  Kroken S  Magnuson JK  Mannhaupt G  Mauceli E  Mewes HW  Mitterbauer R  Muehlbauer G  Münsterkötter M  Nelson D  O'donnell K  Ouellet T  Qi W  Quesneville H  Roncero MI  Seong KY  Tetko IV  Urban M  Waalwijk C  Ward TJ  Yao J  Birren BW  Kistler HC 《Science (New York, N.Y.)》2007,317(5843):1400-1402

We sequenced and annotated the genome of the filamentous fungus Fusarium graminearum, a major pathogen of cultivated cereals. Very few repetitive sequences were detected, and the process of repeat-induced point mutation, in which duplicated sequences are subject to extensive mutation, may partially account for the reduced repeat content and apparent low number of paralogous (ancestrally duplicated) genes. A second strain of F. graminearum contained more than 10,000 single-nucleotide polymorphisms, which were frequently located near telomeres and within other discrete chromosomal segments. Many highly polymorphic regions contained sets of genes implicated in plant-fungus interactions and were unusually divergent, with higher rates of recombination. These regions of genome innovation may result from selection due to interactions of F. graminearum with its plant hosts.  相似文献   

Soil communities (Acari Oribatida; Hexapoda Collembola) in a clay pigeon shooting range     

Massimo Migliorini  Gaia Pigino  Pietro Paolo Fanciulli  Fabio Bernini 《Pedobiologia》2005,49(1):1-13

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A checklist of the outh Italian cultivated plants     

Karl Hammer  Helmut Knüpffer  Pietro Perrino 《Genetic Resources and Crop Evolution》1990,38(3):191-310

Summary Ten collecting missions in South Italy together with an intensive study of literature sources allowed the compilation of a checklist of the cultivated plant species of south Italy. This checklist contains 540 taxa belonging to 520 different species. The alphabetically ordered articles for the taxa contain the botanical name, the plant family, Italian folk names, information about the distribution in the area referring to the regions, data on collections during our missions indicating the regions, too, details of plant uses and references to relevant, mostly Italian literature sources, including reports from our own explorations and papers based on the material collected. Three appendices provide indexes of synonyms and folk names, respectively, with reference to the accepted botanical names, and an index of families and genera. The information given in this checklist was extracted from a database of the cultivated plants of South Italy, a microcomputer-based system. The checklist is a useful tool for further plant genetic resources exploration in Italy, but may be also of interest for ethnobotanists and other researchers engaged in cultivated plants.

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Liste der Kulturpflanzen Süditaliens

Zusammenfassung Zehn Sammelreisen in Süditalien und ein intensives Literaturstudium ermöglichten die Zusammenstellung einer Liste der Kulturpflanzen Süditaliens. Diese Liste enthält 540 Taxa, die 520 verschiedenen Arten angehören. Die alphabetisch geordneten Artikel über die einzelnen Taxa enthalten den botanischen Namen, die Pflanzenfamilie, italienische Volksnamen, Verbreitung im Gebiet nach Regionen aufgeführt, Aufsammlungen während unserer Sammelreisen ebenfalls auf die Regionen bezogen, Angaben über die Nutzung der Pflanzen und Verweise auf wichtige, meist italienische Literaturquellen, einschließlich der Berichte über unsere eigenen Sammelreisen und von Arbeiten, die sich auf unserem Sammelmaterial begründen. Drei Register gestatten das Aufsuchen von Informationen in der Liste nach Synonymen bzw. nach Volksnamen, mit Verweis auf den akzeptierten Namen, und nach Familien und Gattungen. Die in der Liste enthaltene Information wurde aus einer Datenbank der Kulturpflanzen Süditaliens, einem mikrocomputer-gestützten System, ausgewählt. Die Liste kann für die weitere Erfassung pflanzlicher genetischer Ressourcen in Italien nützlich, aber auch für Ethnobotaniker und andere Wissenschaftler, die sich mit Kulturpflanzen befassen, interessant sein.

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A versatile targeted metabolomics method for the rapid quantification of multiple classes of phenolics in fruits and beverages     

Vrhovsek U  Masuero D  Gasperotti M  Franceschi P  Caputi L  Viola R  Mattivi F 《Journal of agricultural and food chemistry》2012,60(36):8831-8840

Compelling evidence of the health benefits of phenolic compounds and their impact on food quality have stimulated the development of analytical methods for the identification and quantification of these compounds in different matrices in recent years. A targeted metabolomics method has been developed for the quantification of 135 phenolics, such as benzoates, phenylpropanoids, coumarins, stilbenes, dihydrochalcones, and flavonoids, in fruit and tea extracts and wine using UPLC/QqQ-MS/MS. Chromatography was optimized to achieve separation of the compounds over a period of 15 min, and MRM transitions were selected for accurate quantification. The method was validated by studying the detection and quantification limits, the linearity ranges, and the intraday and interday repeatability of the analysis. The validated method was applied to the analysis of apples, berries, green tea, and red wine, providing a valuable tool for food quality evaluation and breeding studies.  相似文献   

Controlled reproduction in Anguilla anguilla (L.): comparison between spontaneous spawning and stripping‐insemination approaches          下载免费PDF全文

Andrea Di Biase  Antonio Casalini  Pietro Emmanuele  Michaela Mandelli  Pieter Mark Lokman  Oliviero Mordenti 《Aquaculture Research》2016,47(10):3052-3060

This study aimed to compare the fertility of eggs between artificially matured female silver eels that spawned spontaneously and those that were spawned by manual stripping. The effects of the two methods of spawning on ovulation and fertilization rate were also investigated. For this purpose, 18 wild female European eels captured in Bonello lagoon (North Adriatic Sea) were carp pituitary extract‐injected to undergo sexual maturation and ovulation; a final injection of 17,20β‐dihydroxy‐4‐pregnen‐3‐one (DHP) was administered when at least 30% of the oocytes were fully transparent. After the DHP‐injection, nine eels were transferred to a new closed recirculating aquaculture system, where they were housed with spermiating males (sex ratio 4/1) to allow spontaneous spawning (SPT‐group); the remaining nine eels were transferred to a 250 L tank and ovulation was checked at four‐hourly intervals by manual stripping (STR‐group). The number of eggs per female in the SPT‐group was significantly greater than that in the STR‐group. Furthermore, fertilization rates in the SPT‐group were notably higher than those observed in the STR‐group. Significantly, the best performances were obtained among eels in which at least 50% of oocytes were fully transparent at the time DHP was administered. We conclude that the fertility of eggs from spontaneously spawning eels is superior to that of eggs acquired by strip‐spawning and artificial fertilization.  相似文献   

Messages from Powdery Mildew DNA： How the Interplay with a Host Moulds Pathogen Genomes          下载免费PDF全文

Pietro  D  Spanu 《农业科学学报》2014,13(2):233-236

The genomes of the barley, Arabidopsis and pea powdery mildew are significantly larger than those of related fungi. This is due to an extraordinary expansion of retro-trasposons that are evident as repetitive elements in the sequence. The protein coding genes are fewer than expected due to an overall reduction in the size of gene families, a reduction in the number of paralogs and because of the loss of certain metabolic pathways. Many of these changes have also been observed in the genomes of other taxonomically unrelated obligate biotrophic pathogens. The only group of genes that bucks the trend of gene loss, are those encoding small secreted proteins that bear the hall marks of effectors.  相似文献   

The overexpression of asparagine synthetase A from E. coli affects the nitrogen status in leaves of lettuce (Lactuca sativa L.) and enhances vegetative growth     

Donato Giannino  Chiara Nicolodi  Giulio Testone  Giovanna Frugis  Emanuela Pace  Pietro Santamaria  Mauro Guardasole  Domenico Mariotti 《Euphytica》2008,162(1):11-22

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Occurrence and characterisation of <Emphasis Type="Italic">Rhizoctonia</Emphasis> species causing diseases of ornamental plants in Italy     

Dalia Aiello  Vladimiro Guarnaccia  Pietro T. Formica  Mitsuro Hyakumachi  Giancarlo Polizzi 《European journal of plant pathology / European Foundation for Plant Pathology》2017,148(4):967-982

During surveys conducted in 2010–2012 Rhizoctonia symptoms were observed on 30 ornamental species in different nurseries located in eastern Sicily (Southern Italy). Eighty-eight isolates of Rhizoctonia spp. were obtained from symptomatic leaves, roots and stems. Fifty-six of the isolates were binucleate and 32 were multinucleate Rhizoctonia. Characterisation of anastomosis groups (AGs) was performed using morphological characteristics and sequence analysis of the internal transcribed spacer of ribosomal DNA ( rDNA-ITS) region. Most isolates collected were Rhizoctonia solani AG-4 HG-I (35.2% of all isolates) and one isolate was AG-2-2 IIIB. The binucleate isolates belonged to AG-R (27.3%), AG-A (21.6%), AG-G (12.5%), AG-V (1.1%) and AG-Fb (1.1%). The pathogenicity of 38 representative isolates collected from each host was tested on seedlings or cuttings grown in a growth chamber. All R. solani AG-4 HG-I isolates, most of the binucleate AG-R, AG-A and AG-G and AG-V were pathogenic and reproduced symptoms identical to that observed in nurseries, while binucleate AG-Fb and R. solani AG-2-2 IIIB isolates were nonpathogenic. This is the first report of the occurrence of Rhizoctonia species on some ornamental plants and the first report of binucleate Rhizoctonia AG-R and AG-V in Europe.  相似文献   

Evaluating a filtering and recirculating system to reduce dust drift in simulated sowing of dressed seed and abraded dust particle characteristics          下载免费PDF全文

Marcello Biocca  Daniele Pochi  Roberto Fanigliulo  Pietro Gallo  Patrizio Pulcini  Francesca Marcovecchio  Cinzia Perrino 《Pest management science》2017,73(6):1134-1142

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