Response of a Juvenile Cyprinid,Lake Minnow <Emphasis Type="Italic">Eupallasella perenurus</Emphasis> (Pallas), to Different Diets     

Rafa??KamińskiEmail author  Micha??Korwin-Kossakowski  Jan?Kusznierz  Leszek?Myszkowski  L. Andrzej?Stanny  Jacek?Wolnicki 《Aquaculture International》2005,13(5):479-486

Three commercial starters (Carp Starter, Uni Starter and Perla Plus) and one non-commercial, with frozen Chironomidae larvae as a reference diet, were evaluated for the intensive rearing of juvenile lake minnow Eupallasella perenurus, a cyprinid fish that is critically endangered in Poland. The growth, condition, survival, body deformities, and chemical body composition were studied. The 90-day laboratory experiment was performed at 22 °C with fish that were initially 24.6 mm (mean total lenth (TL)) and 0.11 g (mean body weight (BW)). Satisfactory fish growth was attained with all of the diets; however, the largest (p ≤ 0.05) final size (48.5 mm TL, 1.55 g BW) and the lowest condition coefficient (K = 1.34) were noted in fish fed the non-commercial starter. The final survival rates were very high (97.5–100%). Skeletal deformities (in 74 to 92% fish) were recorded exclusively in fish fed commercial starters. All commercial starters resulted in considerably higher lipid content and lower ash content than did the non-commercial starter and the reference diet. This suggests that both these factors might be responsible for body deformities. The present results proved that only the non-commercial starter is suitable for juvenile E. perenurus rearing under controlled condition, and that none of the commercial starters can be recommended.  相似文献   

Efficacy of Mineral Oil-Insecticide Mixtures for Protection of Potato Tubers Against PVY and PVM     

Sławomir Wróbel 《American Journal of Potato Research》2014,91(6):706-713

The impact of a dozen mixtures of the most commonly applied aphicides: Mospilan 20 SP (acetamiprid), Pirimor 500 (pirimicarb) and Karate Zeon 050 CS (lambda-cyhalothrin), combined with the mineral oil Sunspray 850 EC, was researched in field conditions to assess their effectiveness in limiting potato tuber PVY, PVM and PLRV infection. In spite of the greatest reduction in the number of aphids occurring following application of Mospilan 20 SP, this treatment was not as effective in limiting PVY infection as, for example, applying Sunspray 850 EC mineral oil. Mineral oil, when used on its own or in a mixture with Pirimor 500 WG, was found to be the most effective measure for limiting PVY infection (the incidence of tubers infested with PVY was reduced by 64 % relative to control, i.e. no protection). A slightly weaker effect was observed in the case of a combination of the mineral oil with full doses of Karate Zeon 050 CS with a half of a dose of Mospilan 20 SP insecticide, however only for protection against PVY. A similar trend was observed for PVM even though a significant difference was only observed for Sunspray 850EC?+?Pirimor 500WG. In conclusion, the application of insecticide mixtures with mineral oil in protecting against PVY infection is not always as effective as the application of the oil itself only. Addition of the insecticide may sometimes improve the efficacy of protection, however, due to the extra costs involved, not always does it have to be economical.  相似文献   

Digital radiography (DR) and imaging analysis for evaluating the penetration and distribution of organic substances used in wood conservation     

Joanna Sobczyk  Piotr Frączek  Michał Obarzanowski  Julio M. del Hoyo-Meléndez  Łukasz Bratasz 《Wood Science and Technology》2014,48(5):981-994

Consolidation treatments are commonly employed in museum conservation studios for treating biologically deteriorated wooden cultural heritage objects. Impregnation using either a solvent/resin or a polymerizing system is an example of such an intervention, which is often difficult to describe in terms of its behavior within the object’s structure. A new simple method has been devised to evaluate the effectiveness of these impregnation treatments in terms of spatial distribution of consolidant solution. A combination of digital radiography and imaging analysis has permitted to evaluate the degree of penetration of a consolidant and to determine its location within the artifact by studying the X-ray images taken before, during, and after treatment. The adequacy of polymer solutions or their effectiveness as wood consolidants is properties which are often difficult to investigate due to limited visual access to the interior of the object. The proposed method provides an alternative way of studying these parameters through analysis of X-ray attenuation recorded on two images: taken before and after the conservation treatment. The resulting image is then converted into a two-dimensional map of consolidation agent within the object using a straightforward calibration procedure.  相似文献   

PPR‐B‐31: a new maintainer allele in the male‐fertility restorer gene of radish (Raphanus sativus) Ogura cytoplasm          下载免费PDF全文

Anna Hawliczek‐Strulak  Grzegorz Bartoszewski  Renata Słomnicka  Aleksandra Korzeniewska  Régine Delourme  Iwona Bartkowiak‐Broda  Katarzyna Niemirowicz‐Szczytt 《Plant Breeding》2015,134(5):557-563

The PPR‐B gene is responsible for male‐fertility restoration of the Ogura‐type male‐sterile radish plants, and it is located in the complex Rfo locus in the vicinity of similar PPR‐A gene and PPR‐C pseudogene. The aim of this study was to identify PPR‐B alleles and understand the structure of the Rfo locus in radish breeding lines. Five lines of radish with normal male‐fertile cytoplasm were tested. The entire PPR‐B gene was amplified, sequenced and allelic PPR‐B sequences were identified. The results indicated that the maintainer lines 7, 15 and 21 contained a non‐restoring form of PPR‐B protein. A unique PPR‐B was found in lines 24/15 and 31 that are restorer and maintainer lines, respectively. The substitutions might be responsible for the loss of a restoring function of the PPR‐B‐31 allele. Amplification of the PPR‐A/PPR‐B and PPR‐B/PPR‐C intergenic regions allowed to identify rearrangements within Rfo locus. Obtained results confirm the wide allelic variation within the Rfo locus, as well as high genetic complexity of the fertility restoration mechanism in radish.  相似文献   

Diagnosis of sugar beet (Beta vulgaris L.) nutrient imbalance by DRIS and CND-clr methods at two stages during early growth     

Przemysław Barłóg 《Journal of plant nutrition》2016,39(1):1-16

High yield of sugar beet require adequate mineral nutrition. To be diagnosed across interacting nutrients using appropriate interpretation models, the plant must be sampled at a critical physiological stage. This study aimed to develop and validate norms at the 7-leaf and well-developed rosette stages, for diagnostic purposes using the Diagnosis and Recommended Integrated System (DRIS) and Compositional Nutrient Diagnosis based on centered log ratios (CND-clr). Data on nutrient concentrations and plant performance were obtained from 409 plots in West-Central Poland. With respect to the growth stages, for physiological and practical reasons, the 7-leaf stage is preferable for diagnostic purposes. At this growth stage, the high-yield subpopulation characterized by higher concentration of potassium and sodium compared to other nutrients. CND-clr indices were closely related to DRIS indices (R² > 0.93). The CND-clr indices, however, better explained the differences in the white sugar yield within the validated dataset than the DRIS indices.  相似文献   

Contamination of Forest Soils in the Vicinity of the Sedimentation Pond after Zinc and Lead Ore Flotation (in the Region of Olkusz,Southern Poland)     

Krzaklewski  W.  Barszcz  J.  Małek  S.  kozioł  K.  Pietrzykowski  M. 《Water, air, and soil pollution》2004,159(1):151-164

In the technological processes of ore flotation in the non-ferrous metal industry flotation, tailings constitute up to 98% of the total of the processed material. They are stored in sedimentation ponds as fine-grained drift containing an excess of heavy metals, mainly zinc, cadmium, lead and copper. After the dehydration and drying up of the surface of sedimentation ponds, these metals are carried with dust by the wind and they are accumulated in the vicinity of the ponds in humus horizons of soils causing considerable degradation and even devastation. In this publication, we have presented the results of research on the negative influence of such a structure on the soils of an adjacent fragment of forest and its dependence on the distance from the sedimentation pond. The level of degradation of the soils under research has been assessed on the basis of changes and transformations of the morphological soil profiles, the level of alkalization and accumulation of heavy metals (Zn, Pb, Cd) in the topsoil layers and their translocation in the soil horizons. The following soils have been distinguished in the investigated area: Haplic Podzols distorted and degraded as well as Urbic Anthrosols. The acquired results have served to depict the spatial accumulation of these heavy metals in the topsoil layers. The highest accumulation of heavy metals and the degradation of the forest sites connected with it has occurred in the vicinity of a sedimentation pond drain channel. Soil remediation has been recommended in this area.  相似文献   

Impact of crop production system on the content of macronutrients in potato tubers     

Bogumił Rychcik  Jadwiga Wierzbowska  Milena Kaźmierczak-Pietkiewicz  Arkadiusz Światły 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2020,70(4):349-359

ABSTRACT

The content of macronutrients in potato tubers arouses interest because of their substantial consumption in the world and significant role in elements' budget in human diet. The research objective has been to evaluate the content of macronutrients in tubers of potatoes grown in different systems of crop production in Poland. In 2012–2014, an experiment was conducted to test potato cultivation in three-crop production systems: conventional, integrated and organic. In each of the six-field crop rotation systems, there were five potato cultivars classified into different earliness groups. The following macronutrients in dry mass of potato tubers were determined: N, P, K, Ca, Mg, Na, S and Cl, and the various ratios between them were calculated. The research carried out proved that potatoes production systems affect the chemical composition of tubers, which is additionally modified by whether condition and genetic features of cultivars. Potato tubers from organic farming contained by about 20% less N than tubers from conventional or integrated systems. Potato tubers from organic production system displayed lower content of Ca and Na in comparison with the conventional and integrated systems. The least of P and S were detected in tubers of the potatoes grown conventionally.  相似文献   

Use of Enrichment,and Contamination Factors Together with Geoaccumulation Indexes to Evaluate the Content of Cd,Cu, and Ni in the Rybnik Water Reservoir in Poland   总被引：1，自引：0，他引：1  

Loska  Krzysztof  Cebula  Jan  Pelczar  Jacek  Wiechuła  Danuta  Kwapuliński  Jerzy 《Water, air, and soil pollution》1997,93(1-4):347-365

In this paper, the contamination degree of the Rybnik Reservoir with cadmium, copper and nickel was analyzed. Quality of the water from the reservoir was determined by drawing comparisons between the metal content in the water and both the officially permitted levels (contamination factor) and levels of metals occurring in the water of non-contaminated areas (enrichment factor). Contamination of bottom sediment with chosen metals was analyzed with reference to the metal content in mudstone (geoaccumulation index, enrichment factor, contamination factor). Trends towards changing the metal content in the bottom sediment was analyzed by determining the enrichment factor of the surface layer of the bottom sediments in relation to a deeper layer. Enrichment of the bottom sediments with metals coming from the water was also determined.  相似文献   

The examination of biophysical parameters of skin (transepidermal water loss, skin hydration and pH value) in different body regions of normal cats of both sexes     

Szczepanik MP  Wilkołek PM  Adamek LR  Pomorski ZJ 《Journal of Feline Medicine and Surgery》2011,13(4):224-230

The purpose of this study was to evaluate transepidermal water loss (TEWL), skin hydration and skin pH in normal cats. Twenty shorthaired European cats of both sexes were examined in the study. Measurements were taken from five different sites: the lumbar region, the axillary fossa, the inguinal region, the ventral abdominal region and the left thoracic region. In each of the regions, TEWL, skin hydration and skin pH were measured. The highest TEWL value was observed in the axillary fossa (18.22g/h/m(2)) and the lowest in the lumbar region (10.53g/h/m(2)). The highest skin hydration was found in the inguinal region (18.29CU) and the lowest in the lumbar region (4.62CU). The highest skin pH was observed in the inguinal region (6.64) and the lowest in the lumbar region (6.39). Statistically significant differences in TEWL were observed between the lumbar region and the left side of the thorax region (P=0.016), the axillary fossa (P=0.0004), the ventral region (P=0.005), and the inguinal region (P=0.009). There were significant differences in skin hydration between the lumbar region and the left thorax (P=0.000003), the axillary fossa (P=0.002), the ventral abdomen (P=0.03), and the inguinal region (P=0.0003) as well as between the thorax and the ventral abdomen (P=0.005). TEWL was higher in females (15g/h/m(2)) than in males (4.57g/h/m(2)). Skin hydration was higher in females (13.89CU) than in males (12.28CU). Significant differences were not found between males and females for TEWL and skin hydration. Skin pH was higher in males (6.94) than in females (6.54), which was significant (P=0.004).  相似文献   

Local immunity of the respiratory mucosal system in chickens and turkeys   总被引：1，自引：0，他引：1  

Smiałek M  Tykałowski B  Stenzel T  Koncicki A 《Polish journal of veterinary sciences》2011,14(2):291-297

This review article presents fundamental mechanisms of the local mucosal immunity in selected regions of the respiratory tract in healthy birds and in some pathological conditions. The respiratory system, whose mucosa come into direct contact with microorganisms contaminating inhaled air, has some associated structures, such as Harderian gland (HG), conjunctive-associated lymphoid tissue (CALT) and paranasal glands (PG), whose participation in local mechanisms of the mucosal immunity has been corroborated by numerous scientific studies. The nasal mucosa, with structured clusters of lymphoid tissue (NALT - nasal-associated lymphoid tissue) is the first to come into contact with microorganisms which contaminate inhaled air. Lymphoid nodules, made up of B cells with frequently developed germinal centres (GC), surrounded by a coat of CD4+ cells, are the major NALT structures in chickens, whereas CD8+ cells are situated in the epithelium and in the lamina propria of the nasal cavity mucosa. Studies into respiratory system infections (e.g. Mycoplasma gallisepticum) have shown the reactivity of the tracheal mucosa to infection, despite a lack of essential lymphoid tissue. Bronchus-associated lymphoid tissue (BALT) takes part in bronchial immune processes and its structure, topography and ability to perform defensive function in birds is largely age-dependent. Mature BALT is covered by a delicate layer of epithelial cells, called follicle-associated epithelium (FAE). Germinal centres (GC), surrounded by CD4+ cells are developed in most mature BALT nodules, while CD8+ lymphocytes are dispersed among lymphoid nodules and in the epithelium, and they are rarely present in GC. Macrophages make up the first line of defence mechanisms through which the host rapidly responds to microorganisms and their products in the respiratory mucosal system. Another very important element are polymorphonuclear cells, with heterophils being the most important of them. Phagocytic cells obtained from lung lavages in birds are referred to as FARM (free avian respiratory macrophages). Their number in chickens and turkeys is estimated to be 20 times lower than that in mice and rats, which indicates a deficit in the first-line of defence in the birds' respiratory system. There are numerous B cells and antibody secreting cells (ASC) present throughout the respiratory system in birds. Their role comes down to perform antigen-specific protection by producing antibodies (IgM, IgY or IgA class) as a result of contact with pathogenic factors.  相似文献