Effects of the commercial extract of aronia on oxidative stress in blood platelets isolated from breast cancer patients after the surgery and various phases of the chemotherapy     

Kedzierska M  Olas B  Wachowicz B  Glowacki R  Bald E  Czernek U  Szydłowska-Pazera K  Potemski P  Piekarski J  Jeziorski A 《Fitoterapia》2012,83(2):310-317

Since the extract from berries of Aronia melanocarpa presents antioxidative properties in plasma and in blood platelets, not only from healthy group, but also from patients with benign breast diseases and in patients with invasive breast cancer before surgery, the aim of our present study was to evaluate the oxidative stress by measuring the level of various biomarkers of this process such as the generation of superoxide anion radicals (O(2)(-·)), the amount of carbonyl groups and 3-nitrotyrosine in proteins or the amount of glutathione in blood platelets isolated from breast cancer patients after the surgery and after various phases of the chemotherapy in the presence of A. melanocarpa extract (Aronox) in vitro. We demonstrated in platelet proteins from patients with invasive breast cancer (after the surgery and after various phases of the chemotherapy) higher level of carbonyl groups than in control healthy group. The level of 3-nitrotyrosine in platelet proteins from patients with invasive breast cancer was also significantly higher than in healthy subject group. We observed an increase of other biomarkers of oxidative stress such as O(2)(-·) and a decrease of GSH in platelets from patients with breast cancer (after the surgery and after various phases of the chemotherapy) compared to the healthy group. In model system in vitro our results showed that the commercial extract from berries of A. melanocarpa due to antioxidant action, significantly reduced the oxidative/nitrative stress in platelets from patients with invasive breast cancer caused by the surgery and various phases of the chemotherapy.  相似文献   

Phagocytosis of neutrophils in rabbits infected with antigenic variants of RHD (rabbit haemorrhagic disease) virus     

Niedźwiedzka-Rystwej P  Deptuła W 《Polish journal of veterinary sciences》2012,15(1):143-150

The present study was aimed at determining changes in chosen elements of phagocytosis in rabbits infected with 3 antigenic variants of RHD - Hartmannsdorf, Pv97 and 9905, which differed in haemagglutination ability. The animals were tested for phagocytosis parameters, and the results revealed that the examined strains showed the differences. These variations regarded mainly Pv97 strain, as the intensity of the changes were 5 times stronger in comparison to strain Hartmannsdorf and 9905. As all of the strains examined are signified as antigenic variants, we have stated that this feature does not determine their immunological picture. The results suggest the existence of immunological dissimilarities among strains of the RHD virus, which was revealed for the first time in antigenic variants.  相似文献   

Assessment of treatment of Osteochondrosis dissecans (OCD) of shoulder joint in dogs--the results of two years of experience     

Biezyński J  Skrzypczak P  Piatek A  Kościółek N  Drozdzyńska M 《Polish journal of veterinary sciences》2012,15(2):285-290

Osteochondrosis is a common and clinically important joint disorder that occurs in human and many species of animals such as pigs, horses and dogs. The aim of this article is to present the results of conservative and surgical treatment of Osteochondrosis dissecans (OCD) and to compare the recovery time in relation to race, age and extent of cartilage damage. The study was performed on 36 dogs of both sexes, aged from 4 to 24 months, weighing 12-70 kg, of different breeds (predisposed and non-specific for this disease). All patients underwent clinical, orthopedic and radiological examination through which the location of OCD changes in the shoulder joint and the degree of this problem were established. Based on the examinations the patients were qualified for particular treatment procedures. Control tests were performed after 14, 30 and 60 days, which allowed for a precise evaluation of the progress of therapy. Achieved results indicate that dogs age and the lesions extent in the shoulder joint have an impact on the outcome of treatment. Studies showed that the best therapeutic effects were obtained in dogs aged 6-10 months, which underwent surgery. The study proved that the early diagnosis of OCD strongly influence the recovery rate and may inhibit the development of degenerative changes in the joint (DJD).  相似文献   

Thermally initiated solvent-free radical modification of beech (Fagus sylvatica) wood     

Mariusz Ł. Mamiński  Magdalena Król  Armando G. McDonald  David N. McIlroy  Ishwar B. Niraula  Joanna Czechowska  Paweł Parzuchowski 《Wood Science and Technology》2013,47(5):1019-1031

A procedure for rapidly modifying beech wood using a thermally initiated solvent-free grafting system was examined. In the modification, butyl acrylate and butyl methacrylate were used as vinyl monomers. Free radicals were generated from 2,2′-azobis(2-methylpropionitrile) or benzoyl peroxide at 103 and 180 °C by contact heating of the modified material. Chemical changes in the material were investigated by FTIR and X-ray photoelectron spectroscopies. The modification resulted in decreased surface wetting of the material manifested by increased water contact angles. The hardness of the resultant material decreased, while its color changed by the effect of temperature. It was shown that the approach allowed for efficient thermal-initiated modification of wood with rapid contact heating.  相似文献   

Genetic aspects of age dynamics of a natural Picea abies (L.) Karst. population in the Białowieża Primeval Forest, Poland     

A. Wojnicka-Półtorak  W. Prus-Głowacki  K. Celiński  A. Korczyk 《New Forests》2013,44(6):811-825

We studied temporal changes in the genetic structure and diversity of a naturally regenerated Picea abies (L.) Karst. (Norway spruce) population in the Bia?owie?a Primeval Forest, Poland. The analysis included five age classes of trees: newly germinating seedlings (in laboratory conditions), seedlings up to 3 years old, young trees 4–10 years old, middle-aged trees 11–100 years old, and trees older than 100 years. We conducted genetic analyses of 26 allozyme loci using dormant buds from 405 individuals and 100 embryos. The results showed that the naturally regenerating Norway spruce population is genetically heterogeneous across the studied age classes. As determined by Chi squared tests, there were statistically significant differences in frequencies of alleles and genotypes as well as Wright’s index values (F) among the five age classes. The level of genetic differentiation (pairwise F_ST = 0.5–2.3 %) among the age class groups was equal to levels previously determined for various populations of this species. The Ewens-Watterson test for neutrality showed that one or two loci across different age classes of Norway spruce were affected by the selection process. The distinctiveness of embryo and seedling classes was confirmed by the number of detected alleles, the number of private alleles, the level of observed heterozygosity, and Wright’s index value. The results obtained in the present study indicate there are interesting dynamics of adaptation processes occurring in the natural age-diverse population of Norway spruce in the Bia?owie?a Primeval Forest.  相似文献   

The Seasonal Variation in Bioactive Compounds Content in Juice from Organic and Non-organic Tomatoes   总被引：1，自引：1，他引：0  

Ewelina Hallmann  Janusz Lipowski  Krystian Marszałek  Ewa Rembiałkowska 《Plant foods for human nutrition (Dordrecht, Netherlands)》2013,68(2):171-176

A specific objective of this paper was to evaluate seasonal changes in bioactive compounds level (carotenoids and polyphenols) in juice prepared from organic and non-organic tomatoes in Poland. In the examined tomato juice, the content of dry matter, vitamin C, carotenoids as well as polyphenols (by HPLC method) has been measured. The presented results indicate the impact of the growing system and the year of production on the composition of tomato juice. The organic tomato juice contained significantly more beta-carotene, chlorogenic acid, rutin as well as more total phenolic acids, gallic acid, p-coumaric acid, total flavonoids, quercetin-3-O-glucoside and quercetin in comparison with the non-organic. The tomato juice from 2008 contained significantly more carotenoids and some flavonoids compared to the one produced in 2009, which contained significantly more dry matter, vitamin C, as well as quercetin and it derivatives.  相似文献   

Expression of inflammation-mediated cluster of genes as a new marker of canine mammary malignancy     

K. M. Pawłowski  A. Homa  M. Bulkowska  K. Majchrzak  T. Motyl  M. Król 《Veterinary research communications》2013,37(2):123-131

Because canine mammary tumours constitute a serious clinical problem and there are no good prognostic markers (only histopathological variables are used), the aim of the presented study was to find new malignancy markers as well as to identify intracellular pathways and biological processes characteristic for canine mammary malignancy. We compared gene expression of the most malignant mammary tumours (poorly differentiated cancers of the 3rd grade of malignancy) with less malignant tumours (well differentiated cancers of the 1st grade of malignancy). The results of our study indicated that in dogs the number of tumour-infiltrating myeloid cells or expression of myeloid-specific antigens by cancer cells is related to the cancer progression and may constitute a new marker of malignancy, however further studies in this field are required.  相似文献   

Comparative pharmacokinetics of acetylsalicylic acid and sodium salicylate in chickens and turkeys     

B. Poźniak  M. Świtała  K. Jaworski  P. Okoniewski  P. Niewiński 《British poultry science》2013,54(4):538-544

1. Pharmacokinetics of acetylsalicylic acid (ASA) and sodium salicylate (SS) were assessed following single intravenous (i.v.) and oral administration at doses of 50 mg/kg body weight to chickens and turkeys. Plasma drug concentrations were determined using high-performance liquid chromatography with ultraviolet detection and pharmacokinetic variables were calculated using a non-compartmental model.

2. The mean residence time (MRT) of salicylate (SA) after i.v. administration of SS was 6.08 ± 0.59 and 3.32 ± 0.27 h and after oral administration was 6.95 ± 0.72 and 4.55 ± 0.71 h in chickens and turkeys, respectively. The elimination half-life (T _{1/2 e}) was shorter in turkeys compared with chickens. The value of body clearance (Cl_B) was higher in turkeys than in chickens, but the apparent volume of distribution (V _ss) was similarly low in both species. The bioavailability of SS was complete and the maximal plasma concentration of SA (C _max) after oral administration was 96.93 ± 8.06 and 91.76 ± 9.64 µg/ml, respectively, in chickens and turkeys.

3. The MRT of ASA after iv administration was 0.24 ± 0.08 and 0.24 ± 0.02 h and after oral administration was 0.78 ± 0.25 and 0.59 ± 0.13 h, respectively, in chickens and turkeys. In both species, T _{1/2 e} was very short, Cl_B and V _ss were similar and markedly higher than those of salicylate. The bioavailability of unchanged ASA was low and C _max after oral administration was 6.9 ± 3.6 µg/ml in chickens and 8.6 ± 1.3 µg/ml in turkeys.  相似文献   

Spatio-temporal heterogeneity of soil microbial properties in a conventionally managed arable field     

Piotrowska-Długosz  Anna  Breza-Boruta  Barbara  Długosz  Jacek 《Journal of Soils and Sediments》2019,19(1):345-355

Journal of Soils and Sediments - Knowledge about the spatio-temporal variability of soil microbial properties is crucial in evaluating their structure-function relationship and their impact on...  相似文献   

Fruit Physical Features,Phenolic Compounds Profile and Inhibition Activities of Cranberry Cultivars (Vaccinium macrocarpon) Compared to Wild-Grown Cranberry (Vaccinium oxycoccus)     

Narwojsz  Agnieszka  Tańska  Małgorzata  Mazur  Barbara  Borowska  Eulalia Julitta 《Plant foods for human nutrition (Dordrecht, Netherlands)》2019,74(3):300-306

Plant Foods for Human Nutrition - This study analysed fruits of cranberry cultivars: Ben Lear, Bergman, Early Richard, Pilgrim and Stevens and compared them with wild-grown cranberry fruits. The...  相似文献