SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis     

Zaragoza C  Barrera R  Centeno F  Tapia JA  Durán E  González M  Mañé MC 《Veterinary research》2003,34(2):137-151

Canine leishmaniasis is an endemic disease in the Mediterranean area caused by the protozoan Leishmania infantum, which usually produces renal failure. Sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blot using antibodies to IgG and IgA from dogs were carried out in the urine of 22 dogs with leishmaniasis diagnosed by ELISA and confirmed by PCR, and 20 healthy dogs. The results were compared to renal function laboratory tests and to those from a histopathological study of the kidneys from sick animals that died naturally or were euthanized. Five different bands with molecular weights ranging from 10 to 110 kDa were obtained from the electrophoresis of the urine of healthy dogs. 33.5% of total proteins corresponded to low molecular weight proteins and the other proteins had middle and high molecular weights. However, in the group with leishmaniasis, a maximum of 11 different bands with molecular weights ranging from 10 kDa to 150 kDa were displayed in the electrophoresis of the urine. The urine electrophoretic pattern in the sick dogs was classified as mixed (proteins with high and low molecular weights) because low molecular weight proteins made up 57.9% and the rest of the proteins had middle and high molecular weights. In Western blot, none of the healthy dogs showed excretion of IgG and/or IgA, whereas IgG and IgA were detected in the Western blot of urine of 68% and 55% respectively of dogs with leishmaniasis. The results obtained in the leishmaniasis group agreed with glomerular and tubular damage, which were confirmed by the histopathological findings.  相似文献   

Herbicide resistance in Aster squamatus conferred by a less sensitive form of acetolactate synthase     

Osuna MD  Fischer AJ  De Prado R 《Pest management science》2003,59(11):1210-1216

A biotype of Aster squamatus (Sprengel) Hieronymus with suspected resistance to the ALS-inhibiting herbicide imazapyr was detected in a chicken farm in the province of Seville, Spain, which had been treated once a year with imazapyr for 10 years. Resistance to imazapyr in this biotype was studied using dose-response experiments, absorption and translocation assays, metabolism studies and ALS activity assays. The rate of imazapyr required to inhibit A squamatus growth by 50% (ED50) was 15 times higher for the R (resistant) than for the S (susceptible) biotype. Cross-resistance existed for the ALS-inhibitors imazamox, imazethapyr, amidosulfuron, nicosulfuron, rimsulfuron, triasulfuron and tribenuron, but not for bensulfuron. Control of A squamatus using alternative herbicides was poor with clopyralid, intermediate with quinclorac, amitrole and MCPA, and excellent with 2,4-D, glufosinate and glyphosate. Absorption of [14C]imazapyr increased over time for both the R and S biotypes, and translocation from the treated leaf to shoots and roots was similar in both biotypes, with most of the radioactivity remaining in the treated leaf. No metabolites of imazapyr were detected in either biotype. Sensitivity of the ALS enzyme (target site) to imazapyr was lower for the R biotype (I50(R) = 4.28 x I50(S)). The mechanism of imazapyr resistance in this R biotype appears to be an altered ALS conferring decreased sensitivity to imazapyr at the whole-plant level.  相似文献   

Osteomyelitis associated with an orthopedic implant          下载免费PDF全文

Rahal SC  Mamprim MJ  Caporali EH  Teixeira CR 《The Canadian veterinary journal. La revue veterinaire canadienne》2003,44(7):597-599

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Evaluation of metabolic detoxifying enzyme activities and insecticide resistance in Frankliniella occidentalis     

Maymó AC  Cervera A  Sarabia R  Martínez-Pardo R  Garcerá MD 《Pest management science》2002,58(9):928-934

The western flower thrips Frankliniella occidentalis (Pergande) is a very significant pest of a number of different agricultural crops in the south-east of Spain. The importance of thrips as a pest is not due mainly to the direct damage inflicted on the plant, but to the loss in commercial value which occurs as a consequence of the development of dark spots caused by the tomato spotted wilt virus (TSWV) which they transmit. The economic threshold is therefore almost zero, which enhances the problems of resistance management. The present work is part of a global project that attempts to evaluate the status of insecticide resistance in field populations of thrips obtained from several agricultural crops. We have studied, in either individual or pooled insects, some enzyme systems classically related to detoxification of insecticides: esterase and glutathione-S-transferase (GST). The activity of these enzymes from laboratory populations selected with various classes of insecticides has also been measured using several appropriate substrates. An increase in GST mean activity was found in two field-collected strains. Differences in frequency distributions of esterase and GST activities were found for both field-collected strains and for a laboratory strain selected with acrinathrin. These activities were compared with those of a wild-type reference strain.  相似文献   

Serum α-globulin fraction in horses is related to changes in the acute phase proteins     

Maria Victoria Carapeto DVM  Rafael Barrera DVM  PhD  Maria Cinta Mae DVM  PhD  Concepcin Zaragoza DVM  PhD 《Journal of Equine Veterinary Science》2006,26(3):120-127

Serum electrophoresis is a useful technique to diagnose some diseases characterized by quantitative and qualitative changes in the serum proteins, such as the acute phase proteins. An increased synthesis of acute phase proteins has been associated with acute infection and inflammation. We wanted to study whether gastrointestinal diseases and disorders of the locomotor system in horses produce enough inflammatory reaction so the acute phase proteins can be detected by electrophoresis in serum. Serum total proteins were calculated, and the serum electrophoresis from 63 horses was performed. The horses were classified in 3 groups: Group I (19 healthy horses), Group II (20 horses suffering from inflammatory disorders of the gastrointestinal tract), and Group III (24 horses with inflammatory disorders of the locomotor system). Results indicated an increase of the acute phase proteins only in patent inflammatory disorders that showed a rapid progression. A global increase in those proteins was observed only for α₁-globulins from Group II.  相似文献   

Evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls     

Leila Feiz  Muhammad Irshad  Rafael F Pont-Lezica  Hervé Canut  Elisabeth Jamet 《Plant methods》2006,2(1):10-13

Background  

The ultimate goal of proteomic analysis of a cell compartment should be the exhaustive identification of resident proteins; excluding proteins from other cell compartments. Reaching such a goal closely depends on the reliability of the isolation procedure for the cell compartment of interest. Plant cell walls possess specific difficulties: (i) the lack of a surrounding membrane may result in the loss of cell wall proteins (CWP) during the isolation procedure, (ii) polysaccharide networks of cellulose, hemicelluloses and pectins form potential traps for contaminants such as intracellular proteins. Several reported procedures to isolate cell walls for proteomic analyses led to the isolation of a high proportion (more than 50%) of predicted intracellular proteins. Since isolated cell walls should hold secreted proteins, one can imagine alternative procedures to prepare cell walls containing a lower proportion of contaminant proteins.  相似文献   

Molecular systematics in Chrysanthemum × grandiflorum (Ramat.) Kitamura     

J. Chatterjee  A.K.A. Mandal  S.A. Ranade  Jaime A. Teixeira da Silva  S.K. Datta 《Scientia Horticulturae》2006

An attempt was made to understand the molecular systematics and genetic differences between 10 original chrysanthemum cultivars and 11 mutants. The similarity among the cultivars and mutants varied from 0.17 to 0.90 using RAPD analyses, a simple but efficient method to distinguish cultivars and to assess parentage. Two distinct groups were found. Two cultivars were present as a separate group showing differences from all other cultivars. Mutants with different flower colour could be identified at the molecular level using RAPD technique holding promise to identify unique genes as SCAR markers. A high genetic distance among the different chrysanthemums showed that there exists a possibility of introgressing new and novel genes from the chrysanthemum gene pool.  相似文献   

乳代用品对仔猪生长的影响     

Rafael Cabrera  方正锋  方如君 《国外畜牧学(猪与禽)》2007,27(4):4-6

如果管理得当,给哺乳期的仔猪补充乳可获得多方面的好处。为获得高断奶窝重而对母猪进行高繁殖性能和高营养需求的选择已使饲喂现代的母猪品种成为商业化养猪场中技术最先进的工作。如果青年母猪要  相似文献   

Intestinal obstruction by stones in a turtle.          下载免费PDF全文

S C Rahal  C R Teixeira  G B Castro    L C Vulcano 《The Canadian veterinary journal. La revue veterinaire canadienne》1998,39(6):375-376

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Peripheral blood and abdominal fluid from a dog with abdominal distention     

Ruiz De Gopegui R  Espada Y 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1998,27(2):64-67

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