Combined distemper-adenoviral pneumonia in a dog     

Rodriguez-Tovar LE  Ramírez-Romero R  Valdez-Nava Y  Nevárez-Garza AM  Zárate-Ramos JJ  López A 《The Canadian veterinary journal. La revue veterinaire canadienne》2007,48(6):632-634

A 3 1/2-month-old pug with oculonasal discharge and seizures was submitted for postmortem examination. Grossly, the lungs had cranioventral consolidation, and microscopically, 2 distinct types of inclusion bodies compatible with Canine distemper virus and Canine adenovirus type 2. Presence of both viruses was confirmed via immunohistochemical staining.  相似文献   

Clinico-pathological findings and coagulation disorders in 45 cases of canine babesiosis in Spain     

Ruiz de Gopegui R  Peñalba B  Goicoa A  Espada Y  Fidalgo LE  Espino L 《Veterinary journal (London, England : 1997)》2007,174(1):129-132

A retrospective study of clinical cases of babesiosis in dogs examined at the Veterinary Teaching Hospital, Rof Codina, from January 2003 to October 2004 is presented. The diagnosis was confirmed by direct observation of large piroplasms in stained blood smears. Dogs with concurrent diseases were excluded from the study. Clinical signs, complete blood count, serum biochemistry and hemostasis profiles were obtained. The observed clinical signs were due to hemolytic anemia and inflammatory responses but the most relevant clinico-pathological findings were related to alterations in hemostasis. All dogs presented with thrombocytopenia and 20% had disseminated intravascular coagulation syndrome. Anemia of variable severity was observed in most of the dogs.  相似文献   

Computed tomography (CT) of the lungs of the dog using a helical CT scanner, intravenous iodine contrast medium and different CT windows     

Cardoso L  Gil F  Ramírez G  Teixeira MA  Agut A  Rivero MA  Arencibia A  Vázquez JM 《Anatomia, histologia, embryologia》2007,36(5):328-331

The aim of this study was to determine the accuracy of helical computed tomography (CT) for visualizing pulmonary parenchyma and associated formations in normal dogs. CT scan was performed by using intravenous contrast medium and by applying different types of CT windows: soft tissue and lung windows, and high-resolution computed tomography of the lung. This technique allowed, especially with lung window types, a good view of the parenchyma, bronchial tree, vascular structures and pleural cavity. The selected images, with high anatomical quality and tissue contrast, may be a reference for future clinical studies of this organ. Thus, helical CT is a promising non-invasive method of diagnosing a wide variety of pulmonary diseases in dogs.  相似文献   

Cryptosporidium and Giardia in wild otters (Lutra lutra)     

Méndez-Hermida F  Gómez-Couso H  Romero-Suances R  Ares-Mazás E 《Veterinary parasitology》2007,144(1-2):153-156

A total of 437 faecal samples from wild otter (Lutra lutra) were collected from 161 sites in Galicia (northwest Spain) and were analysed by a direct immunofluorescence antibody test (IFAT). Cryptosporidium sp. oocysts and Giardia sp. cysts were detected in 17 (3.9%) and 30 (6.8%) samples, respectively. The results demonstrate that otters may contribute to the contamination of watercourses, although further studies are required to establish which species or genotypes of Cryptosporidium and Giardia infect these animals and also the significance in terms of public health.  相似文献   

Peripheral blood mononuclear cell supernatants from asymptomatic dogs immunized and experimentally challenged with Leishmania chagasi can stimulate canine macrophages to reduce infection in vitro     

Rodrigues CA  Batista LF  Teixeira MC  Pereira AM  Santos PO  de Sá Oliveira GG  de Freitas LA  Veras PS 《Veterinary parasitology》2007,143(3-4):197-205

Leishmania chagasi is the causative agent of visceral leishmaniasis in both humans and dogs in the New World. The dog is the main domestic reservoir and its infection displays different clinical presentations, from asymptomatic to severe disease. Macrophages play an important role in the control of Leishmania infection. Although it is not an area of intense study, some data suggest a role for canine macrophages in parasite killing by a NO-dependent mechanism. It has been proposed that control of human disease could be possible with the development of an effective vaccine against canine visceral leishmaniasis. Development of a rapid in vitro test to predict animal responses to Leishmania infection or vaccination should be helpful. In this study, an in vitro model was established to test whether peripheral blood mononuclear cell (PBMC) supernatants from dogs immunized with promastigote lysates and infected with L. chagasi promastigotes could stimulate macrophages from healthy dogs in order to control parasite infection. PBMC from a majority of the immunized and experimentally infected dogs expressed IFN-gamma mRNA and secreted IFN-gamma when stimulated with soluble L. chagasi antigen (SLA) in vitro. Additionally, the supernatants from stimulated PBMC were able to reduce the percentage of infected donor macrophages. The results also indicate that parasite killing in this system is dependent on NO, since aminoguanidine (AMG) reversed this effect. This in vitro test appears to be useful for screening animal responses to parasite inoculation as well as studying the lymphocyte effector mechanisms involved in pathogen killing by canine macrophages.  相似文献   

Partitioning of net radiation and evapotranspiration over a superintensive drip-irrigated olive orchard     

Rafael López-Olivari  Samuel Ortega-Farías  Carlos Poblete-Echeverría 《Irrigation Science》2016,34(1):17-31

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Genetic map construction and QTL analysis of nitrogen use efficiency in spinach (Spinacia oleracea L.)     

Rafael Chan-Navarrete  Oene Dolstra  Martijn van Kaauwen  Edith T. Lammerts van Bueren  C. Gerard van der Linden 《Euphytica》2016,208(3):621-636

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Acute Toxicity of Metaldehyde in the Invasive Rice Snail Pomacea canaliculata and Sublethal Effects on Tadpoles of a Non-target Species (Rhinella arenarum)     

Andrés Maximiliano Attademo  Rafael Carlos Lajmanovich  Paola Mariela Peltzer  Celina María Junges 《Water, air, and soil pollution》2016,227(11):400

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Sources,Rates and Time of Nitrogen Application on Maize Crops under No-Tillage System     

J. A. Souza  S. Buzetti  M. C. M. Teixeira Filho 《Communications in Soil Science and Plant Analysis》2016,47(19):2200-2207

Quantitatively, nitrogen (N) is the foremost nutrient for maize crops (Zea mays L.), but the N source to increase the grain productivity still needs more investigation. Thus, the aim of this experiment was to study sources, rates and time of N application on the crop yield and agronomic characteristics of the maize under no-tillage system. The experiment was carried out during two growing seasons on an Oxisol under the factorial 5 × 3 × 3 scheme with five N rates (0, 50, 100, 150, and 200 kg ha^?1) and three sources (ammonium-sulfate-nitrate as inhibitor of the nitrification (ASN+I), ammonium sulfate (AS) and urea); we applied them two times with four replicates: first time at the sowing or later under side dressing when the plants had the six leaves stage. In the first year, the sources of N had no influence on the number of grain line /ear (NGLE), grain number/line (GNL), total number of grain/ear (TNFE), biomass of 100 grain, plant height (PH), height of the first ear insertion (AFEI) and stalk diameter, in contrast with the foliar N content and the crop yield. Early fertilization with N at the sowing time can afford applications as well as the total side dressing. The increase of the rates had positive influence on the N foliar content, plant height and 100 grains biomass. The highest productivities were found with rates above the threshold of 150 kg ha^?1, no matter the sources and the fertilization time.  相似文献   

Biosurfactants Produced by Marine Microorganisms with Therapeutic Applications     

Eduardo J. Gudi?a  José A. Teixeira  Lígia R. Rodrigues 《Marine drugs》2016,14(2)

Marine microorganisms possess unique metabolic and physiological features and are an important source of new biomolecules, such as biosurfactants. Some of these surface-active compounds synthesized by marine microorganisms exhibit antimicrobial, anti-adhesive and anti-biofilm activity against a broad spectrum of human pathogens (including multi-drug resistant pathogens), and could be used instead of existing drugs to treat infections caused by them. In other cases, these biosurfactants show anti-cancer activity, which could be envisaged as an alternative to conventional therapies. However, marine biosurfactants have not been widely explored, mainly due to the difficulties associated with the isolation and growth of their producing microorganisms. Culture-independent techniques (metagenomics) constitute a promising approach to study the genetic resources of otherwise inaccessible marine microorganisms without the requirement of culturing them, and can contribute to the discovery of novel biosurfactants with significant biological activities. This paper reviews the most relevant biosurfactants produced by marine microorganisms with potential therapeutic applications and discusses future perspectives and opportunities to discover novel molecules from marine environments.  相似文献