The importance of lactic acid bacteria for phytate degradation during cereal dough fermentation     

Reale A  Konietzny U  Coppola R  Sorrentino E  Greiner R 《Journal of agricultural and food chemistry》2007,55(8):2993-2997

Lactic acid fermentation of cereal flours resulted in a 100 (rye), 95-100 (wheat), and 39-47% (oat) reduction in phytate content within 24 h. The extent of phytate degradation was shown to be independent from the lactic acid bacteria strain used for fermentation. However, phytate degradation during cereal dough fermentation was positively correlated with endogenous plant phytase activity (rye, 6750 mU g(-1); wheat, 2930 mU g(-1); and oat, 23 mU g(-1)), and heat inactivation of the endogenous cereal phytases prior to lactic acid fermentation resulted in a complete loss of phytate degradation. Phytate degradation was restored after addition of a purified phytase to the liquid dough. Incubation of the cereal flours in buffered solutions resulted in a pH-dependent phytate degradation. The optimum of phytate degradation was shown to be around pH 5.5. Studies on phytase production of 50 lactic acid bacteria strains, previously isolated from sourdoughs, did not result in a significant production of intra- as well as extracellular phytase activity. Therefore, lactic acid bacteria do not participate directly in phytate degradation but provide favorable conditions for the endogenous cereal phytase activity by lowering the pH value.  相似文献   

Lactobacillus rhamnosus as additive for maize and sorghum ensiling   总被引：2，自引：0，他引：2  

Salimei E  Capilongo V  Simoni A  Peiretti PG  Maglieri C  Romano CA  Mannina L  Coppola R  Sorrentino E 《Journal of agricultural and food chemistry》2007,55(23):9600-9607

The effects of Lactobacillus rhamnosus AT195, a potential probiotic microorganism cultured in buffalo "scotta" whey, on chemical and microbiological composition in maize and sorghum ensiling were evaluated. Both crops were harvested, chopped, and treated or not with the selected strain prior to ensiling in fiberglass vertical silos; 90 days after ensiling, silages were sensorially evaluated and sampled. Different chemical components were evaluated both on fresh crops and silages: in particular, the water-soluble carbohydrates content was investigated by high-field NMR spectroscopy and the carbohydrate fermentation profile was performed by GC. Besides phenotypic identification and typing, microbiological studies included Lb. rhamnosus genotype typing by RAPD-PCR. All silages, inoculated or not, were well preserved, as their chemical and microbiological data along with the fermentation profiles showed. The selected strain used as inoculum influenced the lactic acid population of silages and evidenced a good survival performance during the ensiling process of both maize and sorghum. Moreover, the use of Lb. rhamnosus strain efficiently improved the quality of the multifactorial ensiling process by significantly reducing the ammonia nitrogen content of both maize and sorghum silages.  相似文献   

Pathophysiological Responses to Conotoxin Modulation of Voltage-Gated Ion Currents     

Elisabetta Tosti  Raffaele Boni  Alessandra Gallo 《Marine drugs》2022,20(5)

Voltage-gated ion channels are plasma membrane proteins that generate electrical signals following a change in the membrane voltage. Since they are involved in several physiological processes, their dysfunction may be responsible for a series of diseases and pain states particularly related to neuronal and muscular systems. It is well established for decades that bioactive peptides isolated from venoms of marine mollusks belonging to the Conus genus, collectively known as conotoxins, can target different types and isoforms of these channels exerting therapeutic effects and pain relief. For this reason, conotoxins are widely used for either therapeutic purposes or studies on ion channel mechanisms of action disclosure. In addition their positive property, however, conotoxins may generate pathological states through similar ion channel modulation. In this narrative review, we provide pieces of evidence on the pathophysiological impacts that different members of conotoxin families exert by targeting the three most important voltage-gated channels, such as sodium, calcium, and potassium, involved in cellular processes.  相似文献   

Pheromone detection of the introduced forest pest Megaplatypus mutatus (=Platypus mutatus) (Chapuis) (Platypodinae, Curculionidae) in Italy     

Paola Gonzalez-Audino  Raffaele Griffo  Pablo Gatti  Gianni Allegro  Eduardo Zerba 《Agroforestry Systems》2013,87(1):109-115

Megaplatypus mutatus (Chapuis) (=Platypus mutatus), an ambrosia beetle native to South America, attacks standing live trees of a wide range of forest and fruit tree species, and it is particularly damaging to commercial poplar plantations. In 2000, M. mutatus was observed for the first time in Italy, in the province of Caserta, near Naples. The development of a pheromone-based monitoring system, for detecting the spread and for managing M. mutatus infestations, is an important goal for both European and South American control and surveillance programs. Using a three component pheromone blend developed in Argentina into commercial funnel traps we were able to asses the level of dispersion of this pest in the Italian Campania region. Insects were captured in all the plantations suspected of being infested based on the presence of active parental and larval galleries. We also provide the first report of the attack followed by completion of the life cycle of M. mutatus in European hazelnut, Corylus avellana L. (Betulaceae), an important nut species native to Europe and Western Asia.  相似文献   

Quantum cascade surface-emitting photonic crystal laser     

Colombelli R  Srinivasan K  Troccoli M  Painter O  Gmachl CF  Tennant DM  Sergent AM  Sivco DL  Cho AY  Capasso F 《Science (New York, N.Y.)》2003,302(5649):1374-1377

We combine photonic and electronic band structure engineering to create a surface-emitting quantum cascade microcavity laser. A high-index contrast two-dimensional photonic crystal is used to form a micro-resonator that simultaneously provides feedback for laser action and diffracts light vertically from the surface of the semiconductor surface. A top metallic contact allows electrical current injection and provides vertical optical confinement through a bound surface plasmon wave. The miniaturization and tailorable emission properties of this design are potentially important for sensing applications, while electrical pumping can allow new studies of photonic crystal and surface plasmon structures in nonlinear and near-field optics.  相似文献   

The effect of phytates on the growth of rainbow trout (Salmo gairdneri) fed purified diets containing varying quantities of calcium and magnesium     

John Spinelli  Clifford R. Houle  John C. Wekell 《Aquaculture (Amsterdam, Netherlands)》1983,30(1-4):71-83

Rainbow trout were fed for 150 days on four purified diets containing 0 and 0.5% phytic acid. These diets contained increasing increments of Ca (0.92–1.30%) and Mg (0.054–0.085%). The fish fed the diets containing phytic acid had 10% reduced growth and feed conversion. Increasing Ca and Mg content of the diet in the presence of phytic acid did not affect growth and feed conversion. Fish fed diets containing over 1% Ca without phytic acid had a 5% reduction in growth and feed conversion. The zinc and iron levels in the blood of the fish fed the diets containing phytic acid were not significantly different from the controls. The copper levels in the blood decreased in the fish diets with increasing increments of Ca ang Mg, but phytic acid did not influence this change. Increasing increments of Ca and Mg reduced the mean copper levels in the liver from 60 to 37 ppm. Similar reductions were found when phytic acid was included in the diet. Mean liver zinc levels did not significantly vary regardless of the diet fed. In vitro tests confirmed that phytic acid/protein (casein) complexes are only partially hydrolyzed by pepsin. In vivo tests with rainbow trout in which a casein/phytate complex was substituted for casein showed a 6.6% reduction in diet digestibility. It was concluded that the reduced growth in fish fed diets containing phytic acid was related to a reduction in protein availability rather than to an alteration in the bioavailability of Zn, Fe, or Cu.  相似文献   

Nuclear magnetic resonance profiling of wine blends     

Imparato G  Di Paolo E  Braca A  Lamanna R 《Journal of agricultural and food chemistry》2011,59(9):4429-4434

Nuclear magnetic resonance (NMR) profiling is used for characterization of monocultivar binary wine mixtures. Classification and quantification of the relative amount of wine in the mixture are made in two steps. First, each sample is classified as a mixture of a determined type by solving the appropriate classification problem using NMR profiles. The relative amount of the two corresponding monovarietal wines is then evaluated by multilinear regression of a selected set of NMR variables. Linear discriminant analysis (LDA), used in the classification step, gives a very good separation among the different mixture classes. On the other hand, a single layer artificial neural network, used to solve the multilinear problem, gives the relative amount of wine type in the mixture with a precision of about 10%.  相似文献   

Effect of thermally oxidized oil and fasting status on the short-term digestibility of ketolinoleic acids and total oxidized fatty acids in rats     

Olivero-David R  Paduano A  Fogliano V  Vitaglione P  Bastida S  González-Muñoz MJ  Benedí J  Sacchi R  Sánchez-Muniz FJ 《Journal of agricultural and food chemistry》2011,59(9):4684-4691

Western diets contain substantial amounts of lipid oxidation products. The effects of fasting status and oil oxidation on short-term digestibility of oxidized fatty acids (ox-FA) and ketolinoleic acids (keto-LA) of sunflower oils were evaluated. Twelve rats were fasted overnight for 3 days, whereas another 12 rats had free access to diet. From day 4, and for 4 days, two groups of rats, nonfasted (NFT) and fasted (FT), received 1 g/100 g body weight of sunflower oil reused from 40 deep-frying processes, and two control groups of rats, nonfasted (NFC) and fasted (FC), received the same amount of fresh oil. Ox-FA and keto-LA were determined 5 h after the last administration in the various gastrointestinal compartments together with the intraintestinal MDA. Oil digestibility was highest in NFC and lowest in FT rats. NFT and FT rats had higher (at least P < 0.05) intraintestinal MDA, ox-FA, and keto-LA than NFC and FC; MDA and keto-LA concentrations correlated with each other (P < 0.05). Ox-FA and keto-LA levels found in the gastric lumen suggest that digestion contributes to the formation of these compounds. Total ox-FA and keto-LA were efficiently absorbed during the first 5 h after test oil administration, but poorly absorbed in the case of fresh oils. Oil alteration influenced the digestibility of these compounds more than fasting, although the digestibility of oxidized oil was significantly affected by fasting.  相似文献   

Electrochemotherapy for the Treatment of Multiple Melanomas in a Horse     

Enrico P. Spugnini  Gian Lorenzo D' Alterio  Ivan Dotsinsky  Tzvetan Mudrov  Emanuele Dragonetti  Raffaele Murace  Gennaro Citro  Alfonso Baldi 《Journal of Equine Veterinary Science》2011,31(8):430-433

A 22-year-old Camargue mare was referred for showing signs of discomfort during chewing, building up of food in the mouth, and foul odor from the mouth. On physical examination, the mare presented bilateral pigmented labial masses affecting the upper and lower lips, more in the form of plaques at the mucocutaneous junction as well as multiple perivulvar and perianal lumps. The skin lesions were biopsied for histopathologic examination and the clinical suspect of melanoma was confirmed. The owner elected the lesions to be treated with electrochemotherapy using the drug cisplatin. After two sessions, the nodules were shrunk by 50% and the horse’s ability to feed normally was restored: at that time, the owner had the treatment discontinued because of financial issues. The horse is still in partial remission after 1 year from the end of the treatment. Electrochemotherapy can be successfully used to palliate large melanomas in horses.  相似文献   

Harvesting alternatives for mallee agroforestry plantations in Western Australia     

Raffaele Spinelli  Mark Brown  Rick Giles  Dan Huxtable  Ruben Laina Relaño  Natascia Magagnotti 《Agroforestry Systems》2014,88(3):479-487

The authors tested two harvesting systems especially designed for mallee agroforestry plantations on farmland. Both systems were based on versatile forest technology commonly used for conventional logging operations. They differed especially for the felling technology: small-scale drive-to-tree or industrial swing-to-tree equipment. Both systems were tested side-to-side on 12 experimental plots each. The resulting harvesting cost was 22 and 27 AU$ t^?1, for the industrial and the small scale system, respectively. Chipping represented between 60 and 80 % of the overall harvesting cost, and offered much room for improvement. The industrial system always offered the lowest harvesting cost, regardless of annual usage, when equipment mobilisation costs were not considered. The productivity of conventional forestry equipment was strongly dependent on belt stocking and tree size. If the diameter at ankle height dropped below 10 cm, economic viability decreased very rapidly.  相似文献