First report of a geminivirus causing leaf curl disease on Trigonella corniculata evidenced by PCR using degenerate primers     

S. K. Raj  S. K. Pandey  G. Chandra  B. P. Singh  R. K. Gupta 《EPPO Bulletin》2001,31(1):115-117

A leaf curl and mottle disease was observed naturally affecting plants of Trigonella corniculata. A geminivirus was detected in infected plants by PCR using degenerate geminivirus primers and by Southern hybridization with a probe specific to tomato leaf curl begomovirus. This is the first report of a member of the Geminiviridae naturally occurring in T. corniculata.  相似文献   

Isolation of <Emphasis Type="Italic">Penicillium hirsutum</Emphasis> from spoiled,packaged asparagus spears in Japan     

Michihiko?SaitoEmail author  Deepak Raj?Rai  Ryoichi?Masuda 《Journal of General Plant Pathology》2003,69(5):304-306

During storage studies on asparagus spears packed in plastic film and held at 15°C, a Penicillium fungus was associated with subsequent spoilage. On the basis of colony characteristics, the isolate was identified as Penicillium hirsutum Diercks. This report is the first of Penicillium hirsutum causing spoilage of asparagus spears in Japan. Inoculation tests showed that among all the Penicillium species tested in this study only the identified fungus was able to infect asparagus spears.  相似文献   

Effects of field pea (Pisum sativum L.) hay feeding on dry matter intake and milk production of Murrah buffaloes (Bubalus bubalis) fed rice straw ad libitum     

Yoshiaki HAYASHI  Naba Raj DEVKOTA  Hajime KUMAGAI † 《Animal Science Journal》2007,78(2):151-158

To identify the effects of field pea hay (FPH) as a supplement of rice straw (RS) on feed intake and milk production of mid‐late lactation buffaloes in Tarai, Nepal, nine multiparous Murrah were fed a concentrate at 0.6% of their bodyweight (BW) on a concentrate dry matter (DM) basis daily while having ad libitum access to RS. The buffaloes were divided into three groups, and the experiment with three levels of FPH feeding was conducted at the following rate of BW: 0% (T1), 0.5% (T2) and 1.0% (T3) on an FPH DM basis. The DM intake (DMI) was higher in T2 and T3 than in T1. As the amount of FPH was raised, the BW change, crude protein intake (CPI) and total digestible nutrient intake (TDNI) was increased. Although the yield of milk and milk composition did not differ among the treatments, the 7% fat corrected milk yield (FCMY) tended to increase as FPH feeding amount was raised. Although there were no significant differences in FCMY/DMI and FCMY/TDNI among the treatments, FCMY/CPI decreased with FPH feeding. Supplementary FPH increased DMI, CPI and TDNI that might have raised BW, and tended to improve FCMY in mid‐late lactating buffaloes as a result of an increase in TDNI.  相似文献   

Feline Cystinuria Caused by a Missense Mutation in the SLC3A1 Gene          下载免费PDF全文

K. Mizukami  K. Raj  U. Giger 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(1):120-125

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Eicosapentaenoic acid enrichment from sardine oil by argentation chromatography     

Chakraborty K  Raj RP 《Journal of agricultural and food chemistry》2007,55(18):7586-7595

Eicosapentaenoic acid (EPA) derived from chemically hydrolyzed sardine oil was concentrated by urea fractionation using methanol at different temperatures (2, 4, and 6 degrees C) and urea/fatty acid ratios (2:1, 3:1, and 4:1 w/w) and purified by argentation neutral alumina column chromatography. The individual fatty acids were determined as fatty acid methyl esters (FAME) by gas-liquid chromatography and gas chromatography-mass spectroscopy as FAME and N-acyl pyrrolidides. In the mass fragmentation pattern of FAME, the base peak was assigned to be the 1-methoxyethenol moiety (m/z = 74) obtained by McLafferty rearrangement. Formation of the cyclic tropylium ion (m/z = 91) in fatty acids with four or more double bonds was apparent in FAME-PUFAs. The base peak of N-acyl pyrrolidides was the McLafferty rearrangement ion, 1-(pyrrolidin-1-yl)ethenol (m/z = 113). The highest concentration of EPA (47.78%) was obtained at the crystallization temperature of 4 degrees C with a urea/fatty acid ratio of 4:1 (w/w) with 93.74% yield. After complexation of saturated and less unsaturated fatty acids by urea complexation, argentation chromatography resulted in an EPA of high purity (99.6%) with an overall recovery of 54.09% using 50% diethyl ether/n-hexane as eluting solvent. The peroxide (POV) and thiobarbituric acid (TBS) values were found to be highest (4.0 mequiv of O2/kg and 5.2 mg of malondialdehyde/kg, respectively) during urea fractionation at the higher crystallization temperature (6 degrees C) and higher urea/fatty acid ratio (4:1). Keywords: Sardine oil; eicosapentaenoic acid (EPA); fatty acid methyl esters (FAME); urea fractionation; argentation column chromatography.  相似文献   

Biosurfactant-assisted phytoremediation of potentially toxic elements in soil: Green technology for meeting the United Nations Sustainable Development Goals     

Songita SONOWAL  Amy R. NAVA  Sanket J. JOSHI  Siddhartha Narayan BORAH  Nazim F. ISLAM  Soumya PANDIT  Ram PRASAD  Hemen SARMA 《土壤圈》2022,32(1):198-210

Biosurfactants are biomolecules produced by microorganisms, low in toxicity, biodegradable, and relatively easy to synthesize using renewable waste substrates. Biosurfactants are of great importance with a wide and versatile range of applications, including the bioremediation of contaminated sites. Plants may accumulate soil potentially toxic elements(PTEs), and the accumulation efficacy may be further enhanced by the biosurfactants produced by rhizospheric microorganisms. Occasionally, the growth of bacteria slows down in adverse conditions, such as highly contaminated soils with PTEs. In this context,the plant's phytoextraction capacity could be improved by the addition of metal-tolerant bacteria that produce biosurfactants. Several sources, categories,and bioavailability of PTEs in soil are reported in this article, with the focus on the cost-effective and sustainable soil remediation technologies, where biosurfactants are used as a remediation method. How rhizobacterial biosurfactants can improve PTE recovery capabilities of plants is discussed, and the molecular mechanisms in bacterial genomes that support the production of important biosurfactants are listed. The status and cost of commercial biosurfactant production in the international market are also presented.  相似文献   

Investigation into the batch stunning/killing of chickens using carbon dioxide or argon-induced hypoxia   总被引：2，自引：0，他引：2  

A B Mohan Raj  N G Gregory 《Research in veterinary science》1990,49(3):364-366

Three hundred and twenty broilers were stunned for 2 minutes in batches of 10 per crate in four treatments: 45 per cent carbon dioxide; 55 per cent carbon dioxide; 2 per cent oxygen, or 5 per cent oxygen presented as air diluted by argon. The number of survivors and the time to resumption of consciousness were recorded using the time to eye opening and response to comb pinching as indicators of consciousness. In 45 per cent and 55 per cent carbon dioxide and 2 per cent oxygen, 28, none and eight birds, respectively, survived out of 100 in each group. In 5 per cent oxygen the birds were still fully conscious after a 2 minute exposure period, and the test was discontinued. The time to recovery could be rapid after stunning in both the gases. It is suggested that a concentration of 55 per cent carbon dioxide or less than 1 per cent oxygen are required to kill broilers within a 2 minute exposure period, and that care should be taken to ensure that there is sufficient turbulence within the stunning chamber to avoid air pockets being trapped between the birds.  相似文献   

Restriction analysis of the mitochondrial DNA of male-sterile and maintainer lines of pearl millet, Pennisetum americanum L.     

S. V. Munjal  R. K. J. Narayan 《Plant Breeding》1995,114(3):256-258

Mitochondrial DNA from two pairs of cytoplasmic male-sterile (cms) and maintainer lines of pearl millet was investigated by restriction-enzyme analysis and Southern-blot hybridization using three mitochondrial gene probes. Each pair of male-sterile and maintainer lines was of a different nuclear origin. The objective was to distinguish differences in the DNA base-sequence organization of the mitochondrial genomes of cms and maintainer lines from the two sources. Restriction-enzyme analysis revealed differences between the different cms and maintainer lines. Southern-blot hybridization experiments using cloned mitochondrial gene probes further distinguished differences between different lines. It is expected that the restriction-fragment-length polymorphisms revealed in the Southern-blot-hybridization experiments will be useful in distinguishing and classifying cms and maintainer lines obtained from different nuclear backgrounds.  相似文献   

Molecular identification and virulence analysis of AVR genes in rice blast pathogen,Magnaporthe oryzae from Eastern India     

Jahangir Imam  Shamshad Alam  Nimai Prasad Mandal  Pratyoosh Shukla  Tilak Raj Sharma  Mukund Variar 《Euphytica》2015,206(1):21-31

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Long-term electricity production from soil electrogenic bacteria and high-content screening of biofilm formation on the electrodes     

Samuel Raj Babu Arulmani  Vimalan Jayaraj  Solomon RobinsonDavid Jebakumar 《Journal of Soils and Sediments》2016,16(3):831-841

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