First Report of Human Trypanosoma cruzi Infection Attributed to TcBat Genotype     

J. D. Ramírez  C. Hernández  M. Montilla  P. Zambrano  A. C. Flórez  E. Parra  Z. M. Cucunubá 《Zoonoses and public health》2014,61(7):477-479

Chagas disease is an endemic disease of the American continent caused by Trypanosoma cruzi and divided into six discrete typing units (TcI – TcVI). Nearly 10 million people harbour the infection representing a serious issue in public health. Epidemiological surveillance allowed us to detect a bat‐related T. cruzi genotype (henceforth named TcBat) in a 5‐year‐old female living in a forest area in northwestern Colombia. Molecular tools determined a mixed infection of T. cruzi I and TcBat genotypes. This represents the first report of TcBat infection in humans; the epidemiological consequences of this finding are discussed herein.  相似文献   

Changes in the Solubility of Corn Proteins through Interaction with the Arabinoxylans in Extruded Nixtamalized Corn Flour Treated with Xylanase     

Silvia Carolina Moreno-Rivas  Concepción Lorenia Medina-Rodríguez  Patricia Isabel Torres-Chávez  Benjamín Ramírez-Wong  Luis Carlos Platt-Lucero 《Plant foods for human nutrition (Dordrecht, Netherlands)》2014,69(2):148-154

The extrusion process allows the production of nixtamalized corn flour rich in arabinoxylans, which help to prevent cardiovascular and intestinal diseases. During extrusion, physiochemical properties of nixtamalized corn flour are negatively modified. The use of enzymes such as xylanase in order to obtain nixtamalized corn flour using extrusion has been studied as an alternative to reduce these changes in corn flour tortilla. The aim of this research was to evaluate changes in protein solubility of extruded nixtamalized corn flour with and without different concentrations of xylanase enzyme (0.05, 0.075, and 0.1 %, w/w). Soluble proteins of each corn flour were extracted and analyzed by SE-HPLC, while insoluble proteins were determined by the combustion method. In addition, each corn flour was analyzed by scanning electron microscopy (SEM). Results showed that the extruded nixtamalized corn flour, with and without xylanase, increased the protein solubility, and this effect was lower in extruded nixtamalized corn flour with xylanase. Insoluble protein diminished in corn flours either with or without xylanase enzyme. The addition of xylanase reduces the effect that the extrusion process has on the solubility proteins of extruded nixtamalized corn flour.  相似文献   

Biosurfactant-assisted phytoremediation of potentially toxic elements in soil: Green technology for meeting the United Nations Sustainable Development Goals     

Songita SONOWAL  Amy R. NAVA  Sanket J. JOSHI  Siddhartha Narayan BORAH  Nazim F. ISLAM  Soumya PANDIT  Ram PRASAD  Hemen SARMA 《土壤圈》2022,32(1):198-210

Biosurfactants are biomolecules produced by microorganisms, low in toxicity, biodegradable, and relatively easy to synthesize using renewable waste substrates. Biosurfactants are of great importance with a wide and versatile range of applications, including the bioremediation of contaminated sites. Plants may accumulate soil potentially toxic elements(PTEs), and the accumulation efficacy may be further enhanced by the biosurfactants produced by rhizospheric microorganisms. Occasionally, the growth of bacteria slows down in adverse conditions, such as highly contaminated soils with PTEs. In this context,the plant's phytoextraction capacity could be improved by the addition of metal-tolerant bacteria that produce biosurfactants. Several sources, categories,and bioavailability of PTEs in soil are reported in this article, with the focus on the cost-effective and sustainable soil remediation technologies, where biosurfactants are used as a remediation method. How rhizobacterial biosurfactants can improve PTE recovery capabilities of plants is discussed, and the molecular mechanisms in bacterial genomes that support the production of important biosurfactants are listed. The status and cost of commercial biosurfactant production in the international market are also presented.  相似文献   

Semen alterations in porcine rubulavirus-infected boars are related to viral excretion and have implications for artificial insemination   总被引：1，自引：1，他引：0  

Solís M  Ramírez-Mendoza H  Mercado C  Espinosa S  Vallejo V  Reyes-Leyva J  Hernández J 《Research in veterinary science》2007,83(3):403-409

Porcine rubulavirus (PoRV), also known as blue eye disease (BED) of swine, causes respiratory and reproductive problems in pigs at several developmental stages. To study the effect of PoRV infection on semen production, five boars were infected with 1 x 10(6) TCID(50)/ml of PoRV strain PAC-3 and evaluated for 59 days post inoculation (DPI). Infected boars developed reproductive tract pathology that included swelling of the testes and epididymides. Analysis of the semen showed that the infection had little effect on semen production in four animals, but semen from one boar showed severe alterations in sperm concentration, motility, and morphology. When motility was analyzed in BTS-diluted semen after 24, 48, or 72 h, alterations were detected in all boars. Furthermore, viral antigen was detected in semen, the seminal plasma fraction, or sperm fraction from all boars. These results showed that PoRV is excreted via semen and, therefore, artificial insemination is a potential route of dissemination.  相似文献   

Use of culture-derived Fusarium oxysporum f. sp. cubense, race 1 filtrates for rapid and non-destructive in vitro differentiation between resistant and susceptible clones of field-grown banana     

Barbarita Companioni  Mayda Arzola  Yania Rodríguez  Marais Mosqueda  María Cristina Pérez  Orlando Borrás  José Carlos Lorenzo  Ramón Santos 《Euphytica》2003,130(3):341-347

Banana and plantain are among the most important food crops in developing countries but production is threatened by increasing virulent forms of Fusarium oxysporum f. sp. cubense. Chemical control is not economically effective and,therefore, breeding programs are necessary. Traditional field studies of new genotype resistance to this disease are time-consuming and destructive. Therefore,we developed a rapid and non-destructive procedure to differentiate field-grown banana resistant from susceptible clones. This procedure implicates application of culture filtrates of Fusarium oxysporum f. sp. cubense race 1 onto banana leaves. The relationship between duration of the fungal in vitro incubation, and the fungal culture fresh mass, the culture filtrate absorbency, and the Gross Michel (susceptible cultivar)leaf lesion area (after application of the culture filtrate) were similar and at 24day-incubation the highest values of the recorded indicators were observed. A comparison between Gross Michel and FHIA-01(resistant) was also performed. The most relevant differences between cultivars were observed at 48 hours after application of the culture filtrate, and in the middle-aged leaves. The position of the culture filtrate application in the leaf limb (distal, middle, proximal) was not determinant. A wider comparison among banana cultivars confirmed previous results informed by other researchers using different systems to study this plant-fungus interaction. Such a confirmation validates the effectiveness of the procedure described here to select rapid and non-destructively banana resistance to this disease at field level. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Introgression of low temperature tolerance and red rot resistance from Erianthus in sugarcane     

Bakshi Ram  T.V. Sreenivasan  B.K. Sahi  N. Singh 《Euphytica》2001,122(1):145-153

Eighteen clones involving Erianthus, as one of the parents, were evaluated in a randomised block design under sub-tropical conditions of Karnal (Haryana, India). Fifteen characters, which contribute towards stalk yield and juice-quality, were studied. The growth of these clones was monitored during peak winter months of December and January. Significant variability existed among the clones for all the traits studied, except for pol % in juice at 10-month crop age, indicating potentiality of the clones to select desirable types. Nine clones showed significant increase in stalk length during winter months over the better standard Co 1148. Whereas 6 clones developed more number of leaves than Co 1148. Both these characters showed high genetic variability, high heritability and hence, high expected genetic gain. Path analysis revealed that these traits contributed about 16%, 5% and 4% to the total genetic variability in juice quality traits (pol % and CCS %), stalk yield and CCS yield, respectively. Growth in winter months has resulted in an increase in stalk yield and CCS yield. This may help in reducing the yield gap between tropical and sub-tropical regions. Majority of clones showed resistance to red rot disease (major disease of sugarcane in sub-tropical region). Hence, hybridization of Erianthus with sugarcane has resulted in introgression of genes for cold tolerance and red rot resistance. As Erianthus is known for its hardiness, resistance, of these clones, to other abiotic and biotic stresses is to be ascertained. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

The Location Behavior of the Multinational Enterprise: Some Analytical Issues     

Philip McCann  Ram Mudambi 《Growth and change》2004,35(4):491-524

ABSTRACT In the international business literature location behavior has traditionally been analyzed using Dunning's (1977 ) OLI framework, which focuses on the nature, role, and behavior of multinational enterprise (MNE). In this paper it is argued that this approach is now no longer appropriate for discussing the spatial behavior of MNEs, because of the fundamental changes which have taken place either in MNE organization or in the global and institutional environment for foreign direct investment (FDI). At the same time, the paper argues that current location theory from regional economics and economic geography is also largely unsuitable for discussing these issues, such that the spatial behavior of the MNE provides a set of difficult challenges to location analysts. There appears to have been some response to these issues from the international business and management literature, most notably the Porter literature on clusters. However, it is also argued here that this literature provides few, if any, real answers to the problems set by the geographical behavior of the MNE. It is concluded that a fusion of traditional economic geography approaches with a focus on the information and organizational aspects of the firm and the region under consideration may be a way forward for both theory and empirical analysis.  相似文献   

Screening wild plants of Capsicum annuum for resistance to pepper huasteco virus (PHV): Presence of viral DNA and differentiation among populations     

Sergio Hernández-Verdugo  Ramón G. Guevara-González  Rafael F. Rivera-Bustamante  Ken Oyama 《Euphytica》2001,122(1):31-36

Plants collected in thirteen wild populations of Capsicum annuum from Northwest Mexico were tested for resistance to the pepper huasteco begomovirus (formerly subgroup III) (PHV) that is transmitted by the white fly Bemisia tabaci Genadius. Plants were inoculated using both grafting and biolistic methods. Presence of viral DNA was detected by dot blot hybridization and densitometry. Populations varied in their resistance to PHV. Plants of only two of the populations either did not develop disease symptoms or showed very light symptoms after inoculation. In some cases, symptoms appeared several days after inoculation. In plants of these populations viral DNA was detected by dot-blot hybridization assays but they appear to be a good source of resistance (symptomless) for use in breeding programmes. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Wheat grain yield and stability assessed through regional trials in the Eastern Gangetic Plains of South Asia     

Ram C. Sharma  G. Ortiz-Ferrara  J. Crossa  M. R. Bhatta  M. A. Sufian  J. Shoran  A. K. Joshi  R. Chand  Gyanendra Singh  R. Ortiz 《Euphytica》2007,157(3):457-464

Improving the level and stability of grain yield is the primary objective of wheat breeding programs in the Eastern Gangetic Plains (EGP) of South Asia. A regional wheat trial, the Eastern Gangetic Plains Yield Trial (EGPYT), was initiated by CIMMYT in collaboration with national wheat research programs in Bangladesh, Nepal, and India in 1999–2000 to identify wheat genotypes with high and stable grain yield, disease resistance, and superior agronomic traits for the EGP region. A set of 21 wheat experimental genotypes selected from a regional wheat screening nursery in South Asia, three improved widely grown cultivars (Kanchan, PBW343 and Bhrikuti), and one long-term cultivar (Sonalika) were tested at 9–11 sites in six wheat growing seasons (2000–2005) in the EGP. The 21 experimental genotypes were different in each year, whereas the four check cultivars were common. In each year, one or more of the experimental genotypes showed high and stable grain yield and acceptable maturity, plant height, and disease resistance compared to the check cultivars. Three improved cultivars have already been commercially released in the region through EGPYT and many germplasm lines have been used in the breeding programs as parents. Identification of wheat genotypes with high-grain yield in individual sites and high and stable yield across the EGP region underlines their value for regional wheat breeding programs attempting to improve grain yield and agronomic performance.  相似文献   

Marker assisted selection of Ty-2 and Ty-3 carrying tomato lines and their implications in breeding tomato leaf curl disease resistant hybrids     

H. C. Prasanna  Sarvesh P. Kashyap  Ram Krishna  D. P. Sinha  Suresh Reddy  V. G. Malathi 《Euphytica》2015,204(2):407-418

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