三株芽孢杆菌抑菌活性分析及对肉鸡舍空气微生物的影响   总被引：1，自引：0，他引：1  

仝永娟  萨仁娜  张宏福  孙春阳  邢焕  彭易柱  魏忠华  王学静 《中国农业科学》2013,46(20):4344-4353

【目的】分析2株解淀粉芽孢杆菌（Bacillus amyloliquefaciens）和1株苏云金芽孢杆菌（Bacillus thuringiensis）的基本特性以及对大肠埃希氏菌（Escherichia.coli,CVCC1570）的抑制活性;分析3株芽孢杆菌复合制剂对肉鸡舍空气大肠杆菌和金黄色葡萄球菌的抑制作用,为改善畜舍环境提供基础数据。【方法】对3株芽孢杆菌进行形态学观察、镜检和生长曲线测定,以大肠埃希氏菌（CVCC1570）为指示菌株,采用琼脂扩散法对单一菌株的抑菌特性进行测定,筛选出适宜的抑菌浓度;利用三因子三水平的正交试验设计,筛选出最佳抑菌效果的复合芽孢杆菌组合;鸡舍喷洒试验采用单因子设计,分为3个处理组,每个处理设3个重复,分别为空白对照组,化学消毒剂阳性对照组（苯扎氯铵）,喷洒复合芽孢杆菌菌剂处理组。在肉鸡不同日龄检测鸡舍内大肠杆菌和金黄色葡萄球菌的变化规律。【结果】3株芽孢杆菌为革兰氏阳性需氧菌。芽孢杆菌SKN01和芽孢杆菌SKN02生长曲线相近,在培养2h开始进入对数生长期。芽孢杆菌SKN03生长较缓慢,进入对数生长期的时间相对较长。单一菌株对大肠埃希氏菌有效抑菌浓度均为108CFU•mL-1。经正交试验测定,3株芽孢杆菌复合菌液最佳抑菌组合为4×108 CFU•mL-1、4×108 CFU•mL-1、6×108 CFU•mL-1。最佳组合复合芽孢杆菌喷洒鸡舍,能够有效抑制大肠杆菌和金黄色葡萄球菌的生长,在肉鸡生长后期复合制剂的抑菌效果明显。【结论】3株芽孢杆菌能够有效抑制大肠埃希氏菌,同时复合菌液能够有效减少鸡舍有害微生物的数量,起到改善鸡舍环境的作用。  相似文献   

薏米、枸杞保健饮品的研制     

潘俊丽  撒楠  董娜  胡文忠  金黎明 《天津农业科学》2013,(1):36-39

以薏米和枸杞为主要原料制成一种保健饮品。通过正交试验,确定最佳生产配比为:蔗糖5%,柠檬酸0.2%,薏米、枸杞的比例为3∶1。对饮料的可溶性固形物、酸度、还原糖、金属元素、氨基酸含量等指标进行测定。结果为:可溶性固形物5.76%;pH值5.42;还原糖含量17.9mg·L-1。Zn、Mg、Na、Fe、Mn、Ca、K等7种金属元素的含量分别为0.0045,4.7855,35.1020,0.0169,0.0060,4.7429,22.3690g·L-1。Cd、Pb、Cr、Cu、Co等均未测出,且含有16种氨基酸,总量为22.79μg·L-1。  相似文献   

Clinical characterization of Staphylococcus schleiferi infections and identification of risk factors for acquisition of oxacillin-resistant strains in dogs: 225 cases (2003-2009)     

Cain CL  Morris DO  Rankin SC 《Journal of the American Veterinary Medical Association》2011,239(12):1566-1573

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Spatiotemporal variation in snow cover and its effects on grassland phenology on the Mongolian Plateau     

SA Chula  MENG Fanhao  LUO Min  LI Chenhao  WANG Mulan  ADIYA Saruulzaya  BAO Yuhai 《干旱区科学》2021,13(4):332-349

Snow cover is an important water source for vegetation growth in arid and semi-arid areas,and grassland phenology provides valuable information on the response of terrestrial ecosystems to climate change.The Mongolian Plateau features both abundant snow cover resources and typical grassland ecosystems.In recent years,with the intensification of global climate change,the snow cover on the Mongolian Plateau has changed correspondingly,with resulting effects on vegetation growth.In this study,using MOD10A1 snow cover data and MOD13A1 Normalized Difference Vegetation Index(NDVI)data combined with remote sensing(RS)and geographic information system(GIS)techniques,we analyzed the spatiotemporal changes in snow cover and grassland phenology on the Mongolian Plateau from 2001 to 2018.The correlation analysis and grey relation analysis were used to determine the influence of snow cover parameters(snow cover fraction(SCF),snow cover duration(SCD),snow cover onset date(SCOD),and snow cover end date(SCED))on different types of grassland vegetation.The results showed wide snow cover areas,an early start time,a late end time,and a long duration of snow cover over the northern Mongolian Plateau.Additionally,a late start,an early end,and a short duration were observed for grassland phenology,but the southern area showed the opposite trend.The SCF decreased at an annual rate of 0.33%.The SCD was shortened at an annual rate of 0.57 d.The SCOD and SCED in more than half of the study area advanced at annual rates of 5.33 and 5.74 DOY(day of year),respectively.For grassland phenology,the start of the growing season(SOS)advanced at an annual rate of 0.03 DOY,the end of the growing season(EOS)was delayed at an annual rate of 0.14 DOY,and the length of the growing season(LOS)was prolonged at an annual rate of 0.17 d.The SCF,SCD,and SCED in the snow season were significantly positively correlated with the SOS and negatively correlated with the EOS and LOS.The SCOD was significantly negatively correlated with the SOS and positively correlated with the EOS and LOS.The SCD and SCF can directly affect the SOS of grassland vegetation,while the EOS and LOS were obviously influenced by the SCOD and SCED.This study provides a scientific basis for exploring the response trends of alpine vegetation to global climate change.  相似文献   

中间偃麦草、长穗偃麦草及其杂种F1代同工酶研究   总被引：1，自引：0，他引：1  

撒多文  米福贵  刘玉良 《草地学报》2009,17(3):349-353

通过对过氧化物酶(POD)、酯酶(EST)、超氧化物歧化酶(SOD)和多酚氧化酶(PPO)同工酶谱的分析,探讨中间偃麦草(Elytrigia elongata(Host)Nevski)、长穗偃麦草(E.intermedia(Host)Nevski)及其杂种F₁代的酶谱特征和亲缘关系。结果表明:亲本同工酶酶带数目、迁移率、着色程度等差异不大,二者亲缘关系较近。与亲本相比,杂种F₁代存在POD、SOD、PPO酶带丢失现象,但正、反交植株中均产生了一些亲本没有的新生带。杂种F₁代EST酶谱与亲本基本相同,仅着色程度略有差异。杂种F₁代植株的POD、PPO酶谱特征有偏向中间偃麦草的倾向,而SOD酶谱特征倾向于长穗偃麦草。杂种F₁代同工酶具有多态性,可作为遗传标记用于杂种鉴定和后代株系目标性状的选择。  相似文献   

中国苜蓿审定登记品种叶形态特征及变异分析   总被引：2，自引：0，他引：2  

徐春波  于林清  王勇  萨仁 《草地学报》2007,15(3):243-247,268

利用CI-202型叶面积仪研究29个我国审定登记苜蓿品种(Medicago sativa L.)的叶长、叶宽、叶面积、叶周长、叶长/叶宽和叶形指数。结果表明:地方苜蓿品种和育成品种在叶形态方面存在着较大的差异,前者的叶片较后者小;二者叶片形状相似;在6个指标中,叶长宽比变异最大,叶周长变异最小;地方品种的叶面积、叶长、叶周长和叶长宽比的变异系数均高于育成品种,其他2项指标则低于后者;在育成品种中,中兰1号的变异最大,甘农3号苜蓿最小;在地方品种中,蔚县苜蓿的变异最大,河西苜蓿最小;该结果将为今后培育苜蓿新品种提供理论基础。  相似文献   

Pseudomonas fluorescens contamination of a feline packed red blood cell unit and studies of canine units     

Rebecca J. Kessler  Shelley Rankin  Sheri Young  Kathleen O'Shea  Maria Calabrese  Amy Guldin  Nicole Lipson  Donna A. Oakley  Urs Giger 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2010,39(1):29-38

Background: While screening programs have reduced the risk of infectious disease transmission by donors in human and veterinary blood banking, bacterial contamination of blood products has emerged as a major complication in human medicine. Objectives: To describe a Pseudomonas fluorescens (Pf)‐contaminated feline packed RBC (pRBC) unit and experimentally investigate Pf‐contaminated canine pRBCs. Methods: Canine pRBCs were inoculated with Pf‐rich pRBCs from the sentinel feline unit and stored at 4°C or 20°C for 72 hours. Aliquots from the pRBCs were serially evaluated by microscopy, culture, and a eubacterial 16S rRNA real‐time PCR assay. Results: One Pf‐contaminated feline unit turned black after 22 days of storage and was removed from the blood bank; a source was not found, and no other contaminated units were identified. Canine pRBCs spiked with 5 or 25 μL of the sentinel unit became culture‐ and/or 16S PCR‐positive at ≥8 hours at 20°C and 48 hours at 4°C and developed a color change at ≥24 hours. Sensitivity studies indicated that without incubation, inoculation of ≥100 μL Pf‐rich pRBCs was necessary for a positive 16S PCR test result. Conclusions: P. fluorescens grows in stored pRBCs slowly at 4°C and rapidly at 20°C. Screening of blood products for color change, estimating bacterial concentration with microscopy, and 16S PCR testing are simple and fast ways to detect bacteria in stored blood. Aseptic collection, temperature‐controlled storage, and regular visual monitoring of stored units is recommended. Discolored units should not be transfused, but examined for bacterial contamination or other blood product quality problems.  相似文献   

羊免疫布鲁菌病疫苗(S2株)免疫抗体长期监测结果的试验     

苏胜杰  马立峰  戴晓光  申捷  萨茹拉  郭少平  赵心力 《中国兽医杂志》2019,(3):29-32

为探究羊经布鲁菌病疫苗(S2株)免疫后的血清抗体长期动态消长规律,本试验于2012年9月-2015年9月选取阿拉善盟阿拉善左旗96只山羊、100只绵羊作为试验动物,经布鲁菌疫苗(S2株)100亿CFU、200亿CFU不同免疫剂量灌服,分别于免疫前和免疫后不同时间段采血分离血清,应用虎红平板凝集试验和试管凝集试验进行抗体监测。结果表明,绵羊组在一免后20d时,血清抗体阳性率达到最高峰,之后呈下降趋势,150d抗体阳性率降至0%,免疫后180d^360d,绵羊组抗体阳性率一直处于较低水平(0%~4%),二免和三免后不同时间段免疫抗体消长规律与首次免疫基本一致;山羊组抗体阳性率在免疫后20 d时,血清抗体阳性率达到最高峰,之后呈下降趋势,免疫后90 d^360 d,免疫抗体时高时低,起伏较大,无明显规律,二免和三免后不同时间段免疫抗体消长规律与首次免疫基本一致,结果同时表明,100亿CFU和200亿CFU两个免疫剂量组的抗体阳性率无明显差异。  相似文献   

Suspected capture myopathy in an alpaca (Vicugna pacos) following a dog attack     

SA Biasutti 《New Zealand veterinary journal》2019,67(1):52-54

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陕北毛乌素沙地仁用杏丰产栽培技术体系   总被引：6，自引：2，他引：4  

撒文清  魏安智  杨途熙  张睿  杨恒 《经济林研究》2003,21(1):47-49

针对陕北毛乌素沙地地势较为平埋，光热资源丰富，水资源相对充足，但干旱少雨，降水集中，蒸发量大，风沙危害频繁，土质疏松、漏肥、漏水现象严重等特殊的自然条件，提出了适宜该地区采用的仁用杏丰产栽培技术体系。  相似文献