Retention of sperm motility in turbot, Scophthalmus maximus L.: the effects of time from activation, thermal shock and adenosine triphosphate levels     

A. J. GEFFEN  O. FRAYER 《Aquaculture Research》1993,24(2):203-209

Abstract Four parameters were examined in order to define sperm quality in turbot Scophthalmus maximus L., sperm: (1) sperm motility, measured by direct counts of the number of active spermatozoa, expressed as % of total spermatozoa; (2) retention of motility after activation, measured by direct counts, 0–60min after activation, expressed as a % of the initial level of activity; (3) resistance to thermal stress, measured as change in retention of motility, and (4) adenosine phosphate (ATP) concentration, determined for samples of non-activated sperm. The proportion of motile spermatozoa at activation ranged from 34·8% to 97·6% (mean 76·3%) for the individual males tested. Turbot sperm retained on average 52% (range 27–90%) of its initial activity one hour after activation. Sperm samples which were stressed by cooling to –27°C retained only 8·6% (range 0–25%) of initial activity, compared to control samples which retained 49% (range 38–63%) of initial activity. The retention of motility after activation was not significantly related to the initial motility or the levels of ATP. Concentrations of ATP in turbot sperm (mean 0·46mg ATP/10⁶ spermatozoa, equivalent to 9·2nmol ATP/10⁸ spermatozoa) were comparable to those measured in mammals.  相似文献   

Mass mortality of short-tailed shearwaters in the south-eastern Bering Sea during summer 1997   总被引：5，自引：0，他引：5  

C. L. Baduini  †  K. D. Hyrenbach  K. O. Coyle  A. Pinchuk  V. Mendenhall ‡  & G. L. HuntJR 《Fisheries Oceanography》2001,10(1):117-130

During summer 1997, hundreds of thousands of emaciated short-tailed shearwaters ( Puffinus tenuirostris ) died in the south-eastern Bering Sea. Using strip transect methodology, we documented the distribution and abundance of short-tailed shearwaters during cruises conducted prior to, during, and after the die-off, as well as the distributions and abundances of floating carcasses. The distributions and abundances of short-tailed shearwaters in 1997 were similar to those found during the 1970s and early 1980s. In August–September 1997, we observed 163 floating shearwater carcasses, most of which were between St Paul Island and Nunivak Island. We estimated ≈ 190 000 carcasses were afloat in the study area, about 11% of the surveyed population. Between spring (June) and autumn (August/September), mean net body mass of shearwaters decreased by 19%, mean pectoral muscle mass decreased by 14%, and mean percentage body lipid content decreased by 46%, from 15.6% in spring to 8.4% in autumn. Compared with spring, short-tailed shearwater diets broadened in autumn 1997, to include, in addition to adult euphausiids Thysanoessa raschii, juveniles of T. inermis, T. raschii and T. spinifera, crab megalops, fish and squid. We discuss how the ecosystem anomalies in the south-eastern Bering Sea during spring and summer 1997 relate to the mortality event and suggest possible implications of long-term climate change for populations of apex predators in the south-eastern Bering Sea.  相似文献   

Epidermal hyperplasia of walleye, Stizostedion vitreum vitreum (Mitchill), associated with retrovirus-like type-C particles: prevalence, histologic and electron microscopic observations     

T. YAMAMOTO  R. K. KELLY  O. NIELSEN 《Journal of fish diseases》1985,8(5):425-436

Epidermal hyperplasia consisting of discrete translucent raised outgrowths of cells were observed on the skin of walleye, Stizostedion vitreum vitreum (Mitchill), during their spawning period in the spring. The cells constituting the hyperplastic growths were limited to the epidermal layer, and were associated with surface budded, 120-nm-diameter, retrovirus-like particles located in the expanded intercellular spaces. These tumour-like growths were distinct from the other virus-associated skin lesions of walleye including dermal sarcoma, lymphocystis disease and herpesvirus-associated hyperplasia. Lesions could be differentiated by careful observation in the field and comparison of portions of each growth by histologic and electron microscopic observations.  相似文献   

The conduction of wild-animal meat inspection     

O Pr?ndl 《Tier?rztliche Praxis》1977,5(3):395-400

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Transcriptional regulation of fish growth hormone gene     

O. Farchi-Pisanty  H. Sternberg  B. Moav 《Fish physiology and biochemistry》1997,17(1-6):237-246

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Effect of varying density and water level on the spawning response of African catfish Clarias gariepinus: Implications for seed production     

Gamal O. El Naggar  George John  Mahmoud A. Rezk  Waheed Elwan  Mohammed Yehia 《Aquaculture (Amsterdam, Netherlands)》2006,261(3):904-907

The development of a reliable methodology for spawning of African catfish, Clarias gariepinus without the use of hormone injections would greatly improve the prospects of aquaculture in Africa. Earlier work has shown that it is possible to produce C. gariepinus fingerlings by subjecting the broodfish to a physical stress of reduced water depth and/or increased temperature. The hypothesis that C. gariepinus could be induced to spawn through a combined physical stress of lowered water level and increased stocking density was tested in concrete tanks. Three water levels (25, 50 and 75 cm) and three stocking densities (2, 4 and 6 pairs of broodfish at a 1:1 sex ratio in each hapa) were tested. Water depth in the tanks and brood fish density in the hapas affected spawning success. The percentage of spawning females was significantly higher when broodfish were stocked at 2 and 4 pairs in each hapa at water levels of 25 cm or 50 cm. There was no significant difference in spawning response between the 25 and 50 cm depths while a significant difference was seen between the 75 cm and both 25 and 50 cm depths. The results indicate optimum levels and densities for enhancing spawning success in C. gariepinus.  相似文献   

Evaluation of cholesterol additions to a soyabean meal-based diet for juvenile Pacific white shrimp, Penaeus vannamei (Boone), in an outdoor growth trial     

E.O. DUERR  W.A. WALSH 《Aquaculture Nutrition》1996,2(2):111-116

Cholesterol is considered a required nutrient for penaeid shrimps, but an optimal level has not been defined. A 68-day grow-out trial was conducted in 1300-L outdoor tanks in Hawaii, USA, to determine the cholesterol level required in a soyabean meal-based diet for juvenile Pacific white shrimp, Penaeus vannamei (Boone). Test feeds were prepared by adding six cholesterol levels (1.0-10.2 g kg^?1, as-fed basis) to a standard diet high in plant-protein meals, starch and flour. These feeds were provided to the shrimp three times daily. Harvest weights and weekly growth increments varied significantly among diets. The 2.3 g kg^?1 and 4.2 g kg^?1 cholesterol diets yielded better growth than the 1.0 g kg^?1 and 10.2 g kg^?1 diets. Survival and feed conversion ratios did not vary significantly among diets. Significant dietary cholesterol effects on growth of P. vannamei in conditions resembling commercial grow-out indicate that its inclusion in practical diets at more accurately defined levels is necessary. Optimal cholesterol levels for juvenile shrimp may be lower than those now utilized, and may vary relative to the contribution of free sterols from natural foods in shrimp ponds.  相似文献   

Analysis of nutrient flows in integrated intensive aquaculture systems   总被引：12，自引：0，他引：12  

O. Schneider  V. Sereti  E.H. Eding  J.A.J. Verreth 《Aquacultural Engineering》2005,32(3-4):379-401

This paper analyses nutrient conversions, which are taking place in integrated intensive aquaculture systems. In these systems fish is cultured next to other organisms, which are converting otherwise discharged nutrients into valuable products. These conversions are analyzed based on nitrogen and phosphorous balances using a mass balance approach. The analytical concept of this review comprises a hypothetical system design with five modules: (1) the conversion of feed nutrients into fish biomass, the “Fish-Biomass-Converter”; (2) the separation of solid and dissolved fish waste/nutrients; the “Fish-Waste-Processor”; (3) the conversion of dissolved fish waste/nutrients, the “Phototrophic-herbivore-Converter”; (4 and 5) the conversion of solid fish waste, the “Bacterial-Waste-Converter”, or the “Detrivorous-Converter”. In the reviewed examples, fish culture alone retains 20–50% feed nitrogen (N) and 15–65% feed phosphorous (P). The combination of fish culture with phototrophic conversion increases nutrient retention of feed N by 15–50% and feed P by up to 53%. If in addition herbivore consumption is included, nutrient retention decreases by 60–85% feed N and 50–90% feed P. This is according to the general observation of nutrient losses from one trophic level to the next. The conversion of nutrients into bacteria and detrivorous worm biomass contributes only in smaller margins (e.g. 7% feed N and 6% feed P and 0.06% feed N 0.03 × 10⁻³% feed P, respectively). All integrated modules have their specific limitations, which are related to uptake kinetics, nutrient preference, unwanted conversion processes and abiotic factors.  相似文献   

Complete developmental cycle of Myxobolus pseudodispar (Gorbunova) (Myxosporea: Myxobolidae)     

Cs Székely  K Molnár  & O Rácz 《Journal of fish diseases》2001,24(8):461-468

Myxobolus pseudodispar (Gorbunova) is a common parasite of the muscle of roach, Rutilus rutilus L., whereas its actinosporean development occurs in two oligochaete alternate hosts. This paper reports the complete developmental cycle of this parasite in the oligochaete alternate host Tubifex tubifex and the roach. In laboratory experiments, parasite-free T. tubifex specimens were infected by myxospores of M. pseudodispar collected from roach in Lake Balaton. Parasite-free roach fingerlings were infected with floating triactinospores (TAMs) released from oligochaetes on day 69 after challenge. Young plasmodia and spores in roach were first recorded on day 80 post-exposure (p.e.). Myxospores collected from experimentally infected roach initiated a new development in T. tubifex and the resulting TAMs infected roach. No infection of roach resulted from feeding oligochaetes containing mature triactinospores.  相似文献   

Addition of bacteria bioencapsulated in Artemia metanauplii to a rearing system for halibut larvae     

P. Makridis  Ø. Bergh  J. Skjermo  O. Vadstein 《Aquaculture International》2001,9(3):225-235

Theaim of this study was to determine whether it is possible to influence thespecies composition of the gut microflora in 70-days old halibut larvae(Hippoglossus hippoglossus L.) by addition of bacteriabioencapsulated in Artemia franciscana in two shortpulses.Two Vibrio strains, PB 1-11 and PB 6-1 were used. Twotreatments received each strain alone, a third treatment received the twobacterial strains in mixture, whereas in a control treatment no bacteria wereadded. Five fish tanks were included in each treatment. The bacteria werebioencapsulated in Artemia franciscana metanauplii duringashort-term incubation in bacterial suspensions of the specific strains, andwereadded in two pulses, on day 1 and day 10 of the experiment. Addition ofbacteriadid not increase the total number of colony-forming units (CFU) in the larvalgut. The total CFU in the water was lower in tanks added bioencapsulatedbacteria than in the control treatment (p < 0.05). Animmunocolony blot assay was used to measure the numbers of the specificbacteriaadded in samples of water, A. franciscana, and fishlarvae.At the end of the experiment on day 13, the strain PB 1-11 reached on average10% of the total CFU when added alone and 25% when added in mixture with PB6-1.Strain PB 6-1 reached at the end of the experiment 26.8% of the total CFU whenadded alone and 24.2% when added in mixture with PB 1-11, whereas thepercentages of PB 1-11 and PB 6-1 in the control fish were 12.7% and 10.8% ofthe total CFU, respectively. However, the differences between the experimentaltreatments compared with the control group were not significant(p > 0.05). The addition of bioencapsulated bacteria intwo pulses within a ten days period was therefore not sufficient to influencethe species composition of the microflora of the halibut larvae.  相似文献