Influence of cultivar and germination on bioactive amines in soybeans (Glycine max L. Merril)     

Glória MB  Tavares-Neto J  Labanca RA  Carvalho MS 《Journal of agricultural and food chemistry》2005,53(19):7480-7485

The levels of amines in soybeans as affected by cultivar in two consecutive years and by germination were investigated. Spermidine, spermine, putrescine, agmatine, and cadaverine were detected, whereas tyramine, histamine, tryptamine, serotonine, and phenylethylamine were not. Spermidine was the predominant amine followed by spermine. High concentrations of these amines confirmed soybean as a rich source. Cadaverine was confirmed to be inherent to soybean. The percent contribution of spermidine and spermine to total levels was not affected by cultivar in either years. However, amine levels were affected by cultivars in different ways in the consecutive years. Cadaverine was affected more by the cultivar, whereas spermidine, spermine, and agmatine were affected by harvest year. During germination the levels of amines from soybean increased significantly, except for agmatine. Spermidine and spermine accumulated in the cotyledon, whereas cadaverine and putrescine accumulated in the radicle and hypocotyl.  相似文献   

Embryo development and fruit-set in pear induced by untreated and irradiated pollen     

Renata Sniezko  T. Visser 《Euphytica》1987,36(1):287-294

Summary Flowers of the pear cultivars Conference, Doyenné du Comice and Gieser Wildeman were pollinated with untreated and 50-krad-irradiated pollen. Fruitlets were sectioned at regular intervals up to 6 weeks after pollination. Tubes of the irradiated pollen grew slower, but within two weeks nearly as many embryos were initiated as in the case of untreated pollen. Thereafter, the proportion of degenerated embryos increased, although the endosperm sometimes persisted. Six weeks after pollination only about 21% of the seeds induced by the irradiated pollen still contained normal embryos. At or before that time in-vitro culture of the immature seeds will be necessary because few embryos survive until harvest. The irradiated pollen stimulated a rather large parthenocarpic fruit-set, presumably associated with endosperm rather than embryo development during the first 4–6 weeks after pollination. The possibilities of haploid production are discussed.  相似文献   

Effect of delipidant agents during in vitro culture on the development,lipid content,gene expression and cryotolerance of bovine embryos     

Luzia Renata Oliveira Dias  Ligiane Oliveira Leme  José Felipe Warmling Sprícigo  Ivo Pivato  Margot Alves Nunes Dode 《Reproduction in domestic animals》2020,55(1):11-20

In vitro produced embryos are still sensitive to the freezing process which can be explained, in part, by the high-lipid accumulation that characterizes these embryos. Therefore, we aimed to evaluate the effect of delipidating agents, L-carnitine and the trans-10 cis-12 conjugated linoleic acid (CLA) isomer, on blastocyst development, lipid content, gene expression and cryotolerance when added to embryo culture media. Embryos were cultured in four different media: T1: control (n = 616), synthetic oviduct fluid (SOF) media with 5% foetal bovine serum (FBS); T2: L-carnitine (n = 648), SOF medium with 5% FBS and 0.6 mg/ml of L-carnitine; T3: CLA (n = 627), SOF medium with 5% FBS and 100 μM trans-10 cis-12 CLA; and T4: L-carnitine + CLA: (n = 597), SOF medium with 5% FBS plus 0.6 mg/ml L-carnitine and 100 μM trans-10 cis-12 CLA. Supplementation of culture medium with either or both delipidating agents reduced (p < .05) blastocyst rate on D7 (T1 = 49 ± 3.5; T2 = 39 ± 3.0; T3 = 42 ± 3.9 and T4 = 39 ± 3.9), but did not affected gene expression (p > .05). Although embryos cultured in the presence of L-carnitine contained fewer (p < .05) lipid droplets than the control embryos, they showed a lower re-expansion rate 24 hr post-thaw than those (p < .05). In conclusion, although L-carnitine reduced the amount of lipids in cultured embryos, the use of L-carnitine and CLA during in vitro culture was not able to improve the embryo production and the response to cryopreservation.  相似文献   

Management of corneal epithelial defects in a population of mature chuck‐will’s‐widows (Antrostomus carolinensis) in South Florida     

Taryn L. Overton  Robert L. Swinger  Megan E. Climans  Antonia L. Gardner  Amanda L. Grant  Renata M. Schneider  Richard R. Dubielzig  Leandro B. C. Teixeira 《Veterinary ophthalmology》2020,23(3):567-574

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Past,present and future suitable areas for the relict tree Pterocarya fraxinifolia (Juglandaceae): Integrating fossil records,niche modeling,and phylogeography for conservation     

Song  Yi-Gang  Walas  Łukasz  Pietras  Marcin  Sâm  Hoàng Văn  Yousefzadeh  Hamed  Ok  Tolga  Farzaliyev  Vahid  Worobiec  Grzegorz  Worobiec  Elżbieta  Stachowicz-Rybka  Renata  Boratyński  Adam  Boratyńska  Krystyna  Kozlowski  Gregor  Jasińska  Anna K. 《European Journal of Forest Research》2021,140(6):1323-1339

European Journal of Forest Research - Pterocarya fraxinifolia, native to the southern Caucasus and adjacent areas, has been widely introduced in Europe. In this study, we investigate the following:...  相似文献   

Generational growth rate estimates of <Emphasis Type="Italic">Diabrotica virgifera virgifera</Emphasis> populations (Coleoptera: Chrysomelidae)     

Márk Szalai  Judit Papp Komáromi  Renata Bažok  Jasminka Igrc Barčić  József Kiss  Stefan Toepfer 《Journal of pest science》2011,84(1):133-142

Modelling population dynamics of the maize pest Diabrotica virgifera virgifera LeConte (western corn rootworm; Coleoptera: Chrysomelidae) requires knowledge on the growth rate (=net reproductive rate) of the species. We investigated the generational (=annual) growth rate of D. v. virgifera in isolated maize fields in southern Hungary and eastern Croatia over several years. The population densities of D. v. virgifera were assessed by absolute counts of emerging adults in 90 gauze cages per study field. Emergence ranged from 1.3 to 30.7 adults per m² in continuous maize field sections, and from 0.3 to 5.1 adults per m² in adjacent first-year maize sections. The annual growth rates of D. v. virgifera ranged from 0.5 to 13, and averaged in close to 4. These experimentally assessed growth rates could complement growth estimates in population dynamic models, particularly those for forecasting the population growth to economic thresholds or for estimating population build-ups after new introductions of this alien species in Europe. As an example, the determined growth rate was used to estimate that the first documented successful introduction of this species into Europe occurred between 1979 and 1984, which is 8–13 years before the detection of this species and its larval damage in maize fields near Belgrade, Serbia, in 1992.  相似文献   

Hydroelectric reservoir inundation (Rio Madeira Basin, Amazon) and changes in traditional lifestyle: impact on growth and neurodevelopment of pre-school children     

Marques RC  Dórea JG  McManus C  Leão RS  Brandão KG  Marques RC  Vieira IH  Guimarães JR  Malm O 《Public health nutrition》2011,14(4):661-669

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Parasites in pet reptiles     

Aleksandra Vergles Rataj  Renata Lindtner-Knific  Ksenija Vlahovi?  Ur?ka Mavri  Alenka Dov? 《Acta veterinaria Scandinavica》2011,53(1):33

Exotic reptiles originating from the wild can be carriers of many different pathogens and some of them can infect humans. Reptiles imported into Slovenia from 2000 to 2005, specimens of native species taken from the wild and captive bred species were investigated. A total of 949 reptiles (55 snakes, 331 lizards and 563 turtles), belonging to 68 different species, were examined for the presence of endoparasites and ectoparasites. Twelve different groups (Nematoda (5), Trematoda (1), Acanthocephala (1), Pentastomida (1) and Protozoa (4)) of endoparasites were determined in 26 (47.3%) of 55 examined snakes. In snakes two different species of ectoparasites were also found. Among the tested lizards eighteen different groups (Nematoda (8), Cestoda (1), Trematoda (1), Acanthocephala (1), Pentastomida (1) and Protozoa (6)) of endoparasites in 252 (76.1%) of 331 examined animals were found. One Trombiculid ectoparasite was determined. In 563 of examined turtles eight different groups (Nematoda (4), Cestoda (1), Trematoda (1) and Protozoa (2)) of endoparasites were determined in 498 (88.5%) animals. In examined turtles three different species of ectoparasites were seen. The established prevalence of various parasites in reptiles used as pet animals indicates the need for examination on specific pathogens prior to introduction to owners.  相似文献   

Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status     

Menezes-Souza D  Corrêa-Oliveira R  Guerra-Sá R  Giunchetti RC  Teixeira-Carvalho A  Martins-Filho OA  Oliveira GC  Reis AB 《Veterinary parasitology》2011,177(1-2):39-49

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Phylogenetic analysis of Foot-and-Mouth Disease Virus type O circulating in the Andean region of South America during 2002-2008     

Malirat V  Bergmann IE  Campos Rde M  Salgado G  Sánchez C  Conde F  Quiroga JL  Ortiz S 《Veterinary microbiology》2011,152(1-2):74-87

At present, Foot-and-Mouth Disease (FMD) has been successfully controlled in most territories of South America, where only Ecuador and Venezuela remain as endemic countries. In this context, the precise characterization of circulating viruses is of utmost importance. This work describes the first molecular epidemiology study performed with the complete VP(1)-coding region of 114 field isolates of FMD virus (FMDV) type O, collected in the Andean countries mainly during 2002-2008. Sequences were aligned and compared to isolates responsible for emergencies in the Southern Cone of the continent between the years 2000 and 2006, and to other representative type O viruses worldwide. The results showed that FMD type O viruses isolated in South America and analyzed up to date are placed in 11 different lineages within the Euro SA topotype. Five of these lineages included viruses circulating in Ecuador and Venezuela during 2002-2008. The last emergencies reported in already-free areas in the Andean region, showed close relationships with viruses circulating in these endemic countries. Andean lineages showed a clear separation from the unique lineage containing viruses responsible for the emergencies in the Southern Cone, reflecting the different livestock circuits and providing evidence that support the ecosystem dynamics in the region. A wide geographical dissemination of the same strain in short time intervals has been observed, pointing to animal movements as the most significant risk parameter. This fact, together with an important generation of viral variants in areas under weak control strategies, reinforce the need of stronger official controls, as well as for establishing multinational cooperative measures in the border areas.  相似文献