Periodicity and chaos in coupled nonlinear oscillators     

Gollub JP  Brunner TO  Danly BG 《Science (New York, N.Y.)》1978,200(4337):48-50

A system of coupled tunnel diode relaxation oscillators shows a variety of complex periodic states as the external voltage is varied. The existence of chaotic or nonperiodic states is more dependent on the nature of the coupling than on the number of degrees of freedom. A simple but accurate numerical model shows many of the phenomena observed experimentally.  相似文献   

Seroprevalence of Toxoplasma gondii and Neospora caninum infection of cats in Hungary     

Hornok S  Edelhofer R  Joachim A  Farkas R  Berta K  Répási A  Lakatos B 《Acta veterinaria Hungarica》2008,56(1):81-88

Blood samples were collected from 330 cats in Hungary in order to evaluate their seroconversion to Toxoplasma gondii and Neospora caninum using the indirect fluorescent antibody test (IFAT). The overall prevalence of toxoplasmosis was 47.6%, the prevalence being 22.4% among urban, 50% among suburban and 61.3% among rural animals. Significantly more cats had high IFAT titres (1:640 to 1:5120) in the countryside. Female cats were more frequently infected with T. gondii than males (53.3% vs. 39.3%), and seropositivity increased with the age of animals. The prevalence (0.6%) and titre (1:40) of antibodies to N. caninum was low. Sixty-two cats were also screened for seroconversion to feline infectious peritonitis (FIP) virus. Higher titres to T. gondii were more frequently detected among FIP-positive cats, but this difference was non-significant due to the small number of cats with concurrent infection.  相似文献   

Induction of blastogenesis in bovine peripheral blood lymphocytes by oxidation with sodium periodate     

C J Brunner  D W Johnson  C C Muscoplat 《American journal of veterinary research》1979,40(10):1386-1390

Suitable treatment and culture conditions are defined for the induction of blast transformation in bovine peripheral blood lymphocytes by oxidation with sodium metaperiodate (NaIO4). Stimulation with NaIO4 required slight modification of techniques used routinely for activation of lymphocytes in vitro with lectins and antigens. Gradient-separated mononuclear leukocytes responded with maximal [3H]TdR incorporation after oxidation with 0.50 to 1.0 mM NaIO4 for 30 minutes at 25 C. Oxidized cells cultured at 1 to 2 X 10(6)/ml responded better than cells cultured at any other concentration, when compared with untreated cells. Blastogenesis in response to oxidation reached its maximum rate within 48 hours of treatment, after which it declined rapidly. Partial removal of glass wool-adherent cells reduced periodate-triggered blastogenesis by 95%, but did not significantly affect activation with phytohemagglutinin, concanavalin A, pokeweed mitogen, or purified protein derivative. Reintroduction of macrophages restored responses to their precolumn level. Oxidation with NaIO4 provided a simple, rapid means of inducing blastogenesis in bovine lymphocytes. Manipulation of the well-defined triggering conditions may help to explain the mechanisms involved in lymphocyte activation.  相似文献   

Streptococcus zooepidemicus infection in a guinea pig colony     

A Weber  H P Brunner 《Berliner und Münchener tier?rztliche Wochenschrift》1979,92(17):336-339

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First Record of A and B Type Beet necrotic yellow vein virus in Sugar Beets in Sweden     

Britt-Louise Lennefors  Klas Lindsten  Renate Koenig 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(2):199-201

Natural infections of sugar beet with Beet necrotic yellow vein virus (BNYVV), the causal agent of rhizomania, have been detected for the first time in Sweden in two small areas, one on the island of Öland and one in the Southeastern part of Scania. Single strand conformation polymorphism analyses of PCR products revealed that the infections on Öland were produced by A type BNYVV, whereas those in Scania were caused by the B type. This suggests that BNYVV has been introduced into Sweden at least twice. Alternatively, the virus may have invaded sugar beet from unknown native hosts. BNYVV RNA 5 was not detected in the samples investigated.  相似文献   

Polyphenols in the woody roots of Norway spruce and European beech reduce TTC     

Richter AK  Frossard E  Brunner I 《Tree physiology》2007,27(1):155-160

A common method to determine the vitality of fine root tissue is the measurement of respiratory activity with triphenyltetrazolium chloride (TTC). The colorless TTC is reduced to the red-colored triphenyl formazan (TF) as a result of the dehydrogenase activity of the mitochondrial respiratory chain. However, measurements with woody fine roots of adult Norway spruce and European beech trees showed that dead control roots had a high potential to react with TTC. High reactivity was found in boiled fine roots and the bark of coarse roots, but not in the boiled wood of coarse roots. By sequential extraction of dried and ground adult Norway spruce fine roots, reactivity with TTC was reduced by about 75% (water extraction), 93% (water/methanol extraction) and 94% (water/acetone extraction). The water extract reacted with TTC in the same way as polyphenols such as lignin, catechin and epicatechin. Boiling did not affect the extent to which fine roots of adult trees reduced TTC, whereas it greatly reduced TTC reduction by seedling roots. Application of the TTC test to roots of spruce seedlings subjected to increasing drought showed a progressive decrease in TTC reduction. The decrease in TTC reduction was paralleled by a reduction in O(2) consumption, thus supporting the conclusion that for roots with a low polyphenol content the TTC test provides a valid assessment of tissue vitality. Our results suggest, however, that the TTC test should not be applied to the fine roots of adult trees because of their high content of polyphenolic compounds whose reaction with TTC masks changes in TTC reduction due to changes in the respiratory capacity of the tissue.  相似文献   

AFLP assessment of the genetic diversity of Calotropis procera (Apocynaceae) in the West Africa region (Benin)     

D. Angelo R. Agossou Yao  Yves Sprycha  Stefan Porembski  Renate Horn 《Genetic Resources and Crop Evolution》2015,62(6):863-878

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Histopathological findings,phenotyping of inflammatory cells,and expression of markers of nitritative injury in joint tissue samples from calves after vaccination and intraarticular challenge with Mycoplasma bovis strain 1067     

Vemuri Rama Devi  Fran?ois Poumarat  Dominique Le Grand  Renate Rosengarten  Kathrin Hermeyer  Marion Hewicker-Trautwein 《Acta veterinaria Scandinavica》2014,56(1):45

Background

The pathogenesis of caseonecrotic lesions developing in lungs and joints of calves infected with Mycoplasma bovis is not clear and attempts to prevent M. bovis-induced disease by vaccines have been largely unsuccessful. In this investigation, joint samples from 4 calves, i.e. 2 vaccinated and 2 non-vaccinated, of a vaccination experiment with intraarticular challenge were examined. The aim was to characterize the histopathological findings, the phenotypes of inflammatory cells, the expression of class II major histocompatibility complex (MHC class II) molecules, and the expression of markers for nitritative stress, i.e. inducible nitric oxide synthase (iNOS) and nitrotyrosine (NT), in synovial membrane samples from these calves. Furthermore, the samples were examined for M. bovis antigens including variable surface protein (Vsp) antigens and M. bovis organisms by cultivation techniques.

Results

The inoculated joints of all 4 calves had caseonecrotic and inflammatory lesions. Necrotic foci were demarcated by phagocytic cells, i.e. macrophages and neutrophilic granulocytes, and by T and B lymphocytes. The presence of M. bovis antigens in necrotic tissue lesions was associated with expression of iNOS and NT by macrophages. Only single macrophages demarcating the necrotic foci were positive for MHC class II. Microbiological results revealed that M. bovis had spread to approximately 27% of the non-inoculated joints. Differences in extent or severity between the lesions in samples from vaccinated and non-vaccinated animals were not seen.

Conclusions

The results suggest that nitritative injury, as in pneumonic lung tissue of M. bovis-infected calves, is involved in the development of caseonecrotic joint lesions. Only single macrophages were positive for MHC class II indicating down-regulation of antigen-presenting mechanisms possibly caused by local production of iNOS and NO by infiltrating macrophages.  相似文献   

Mutations in the pericentrin (PCNT) gene cause primordial dwarfism     

Rauch A  Thiel CT  Schindler D  Wick U  Crow YJ  Ekici AB  van Essen AJ  Goecke TO  Al-Gazali L  Chrzanowska KH  Zweier C  Brunner HG  Becker K  Curry CJ  Dallapiccola B  Devriendt K  Dörfler A  Kinning E  Megarbane A  Meinecke P  Semple RK  Spranger S  Toutain A  Trembath RC  Voss E  Wilson L  Hennekam R  de Zegher F  Dörr HG  Reis A 《Science (New York, N.Y.)》2008,319(5864):816-819

Fundamental processes influencing human growth can be revealed by studying extreme short stature. Using genetic linkage analysis, we find that biallelic loss-of-function mutations in the centrosomal pericentrin (PCNT) gene on chromosome 21q22.3 cause microcephalic osteodysplastic primordial dwarfism type II (MOPD II) in 25 patients. Adults with this rare inherited condition have an average height of 100 centimeters and a brain size comparable to that of a 3-month-old baby, but are of near-normal intelligence. Absence of PCNT results in disorganized mitotic spindles and missegregation of chromosomes. Mutations in related genes are known to cause primary microcephaly (MCPH1, CDK5RAP2, ASPM, and CENPJ).  相似文献   

Vacuum-induced transparency     

Tanji-Suzuki H  Chen W  Landig R  Simon J  Vuletić V 《Science (New York, N.Y.)》2011,333(6047):1266-1269

Photons are excellent information carriers but normally pass through each other without consequence. Engineered interactions between photons would enable applications as varied as quantum information processing and simulation of condensed matter systems. Using an ensemble of cold atoms strongly coupled to an optical cavity, we found that the transmission of light through a medium may be controlled with few photons and even by the electromagnetic vacuum field. The vacuum induces a group delay of 25 nanoseconds on the input optical pulse, corresponding to a light velocity of 1600 meters per second, and a transparency of 40% that increases to 80% when the cavity is filled with 10 photons. This strongly nonlinear effect provides prospects for advanced quantum devices such as photon number-state filters.  相似文献