Quantitative risk assessment for reclaimed wastewater irrigation on paddy rice field in Korea     

H. P. Rhee  C. G. Yoon  Y. K. Son  J. H. Jang 《Paddy and Water Environment》2011,9(2):183-191

Water shortage has become an important issue for Korean agriculture. Korea suffers from a limited agricultural water supply, and wastewater reuse has been recommended as an alternative solution. This study examined the concentrations of toxic heavy metals and Escherichia coli in a paddy rice field irrigated with reclaimed wastewater to evaluate the risk to farmers. Most epidemiological studies have been based on upland fields, and therefore may not be directly applicable to paddy fields. In this study, a Beta-Poisson model was used to estimate the microbial risk of pathogen ingestion. The risk value increased significantly after irrigation and precipitation. The results of the microbial risk assessment showed that risk values of groundwater and reclaimed wastewater irrigation were lower than the values of effluent directly from wastewater treatment plants. The monitoring results of heavy metals for each irrigated paddy fields did not show specific tendency. A risk assessment for toxic heavy metals was performed according to various exposure pathways; however, the results of the carcinogenic and noncarcinogenic risk estimation showed that the risk from reclaimed wastewater-irrigated paddy fields was the lowest.  相似文献   

Identification of genes contributing to the intracellular replication of Brucella abortus within HeLa and RAW 264.7 cells     

Kim DH  Lim JJ  Lee JJ  Kim DG  Lee HJ  Min W  Kim KD  Chang HH  Rhee MH  Watarai M  Kim S 《Veterinary microbiology》2012,158(3-4):322-328

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Improved development of ICR mouse 2-cell embryos by the addition of amino acids to a serum-, phosphate-and glucose-free medium     

Cho J  Park S  Chung H  Shim H  Lee B  Rhee K  Kang S  Han J  Lee C  Lee E  Hwang W  Lim J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(9):797-801

This study was conducted to evaluate how exogenous amino acids could affect preimplantation development of ICR mouse embryos. Two-cell embryos collected from naturally mated mice were cultured in amino acid-, glucose- and phosphate-free preimplantation (P)-1 medium. In Experiments 1, 19 amino acids (aa; 1% and 0.5% of MEM essential and nonessential amino acid solutions, respectively) were added to P-1 medium supplemented with either fatty acid-free bovine serum albumin (BSA; 3 mg/mL) or human follicular fluid (hFF; 10%). Regardless of BSA or hFF addition, embryo development to the morula (84 to 86% vs. 97 to 100%) and the blastocyst (54% vs. 93 to 94%) stages was significantly (P<0.05) enhanced by the addition of aa compared with no addition. In Experiment 2, the cell number of blastomeres and inner cell mass (ICM) cells in blastocysts and the ratio of ICM cell to trophectodermal cell (TE) were evaluated after aa addition. In both BSA- and hFF-containing P-1 medium, a significant increase in total blastomere number were found after aa addition (47 to 52 vs. 62 to 63 cells) compared with no addition. However, the ICM/TE ratio was not significantly affected by aa supplementation in both media, while ICM cell number was greatly increased after aa addition in hFF-containing medium (12 vs. 17 cells). When blastocysts were further cultured up to 162 hr post-hCG injection, development to the hatched blastocyst stage was significantly promoted by aa addition (0% vs. 11 to 20%) in both BSA- and hFF-containing media. In conclusion, aa significantly promote the preimplantation development to the hatched blastocyst stage and such effect mainly exerted on supporting blastomere proliferation.  相似文献   

Pregnancy Rates to Fixed Embryo Transfer of Vitrified IVP Bos indicus,Bos taurus or Bos indicus × Bos taurus Embryos          下载免费PDF全文

LSR Marinho  BV Sanches  CO Rosa  JH Tannura  AG Rigo  AC Basso  JHF Pontes  MM Seneda 《Reproduction in domestic animals》2015,50(5):807-811

The pregnancy rates obtained after the transfer of cryopreserved in vitro‐produced (IVP) embryos are usually low and/or inconsistent. The objective of this study was to evaluate the pregnancy rates of Holstein, Gyr and Holstein × Gyr cattle after the transfer of vitrified IVP embryos produced with X‐sorted sperm. Seventy‐two Gyr and 703 Holstein females were subjected to ovum pickup (OPU) sessions, followed by in vitro embryo production using semen from sires of the same breeds. Embryos (1636 Holstein, 241 Gyr and 1515 Holstein × Gyr) were exposed to forskolin for 48 h prior to vitrification. The pregnancy rate achieved with Gyr dam and sire was 46.1%, which was similar (p = 0.11) to that of Holstein dam and Gyr sire (40.3%). Crossing Gyr dams with Holstein sires resulted in a pregnancy rate of 38.9% and did not differ (p = 0.58) from the pregnancy rate obtained with the cross between Holstein dams and Gyr sires. The rate obtained with Holstein dam and sire was 32.5%. The average pregnancy rate was 36.6%, and no difference was found in the proportion of female foetuses (88.8%, in average) among breeds (p > 0.05). In conclusion, transfer of cryopreserved X‐sorted embryos represents an interesting choice for dairy cattle. Despite the small differences between pregnancy rates, we highlight the efficiency of this strategy for all of the racial groups studied.  相似文献   

Evaluation of Different Inoculation Methods for Screening of Sclerotinia rot and Phytophthora blight in Perilla Germplasm     

Afroz  Tania  Lee  Ho-Sun  Jeon  Young-Ah  Sung  Jung-Sook  Rhee  Ju-Hee  Assefa  Awraris Derbie  Noh  Jaejong  Hwang  Aejin  Hur  On-Sook  Ro  Na-Young  Lee  Jae-Eun  Lee  Myung-Chul 《Journal of Crop Science and Biotechnology》2019,22(2):177-183

Journal of Crop Science and Biotechnology - Sclerotinia rot and Phytophthora blight are devastating diseases of perilla that can cause huge loss of perilla production. Screening disease resistant...  相似文献   

Do Health Promoting Compounds of Flaxseed Attenuate Weight Gain Via Modulation of Obesity Gene Expression?     

Evenocheck  A.  Rhee  Y.  Hall  C. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(3):441-445

Plant Foods for Human Nutrition - Diet-induced obesity (DIO) has been shown to increase DNA methyltransferases (DNMTs) expression and DNMTs binding at obesity-associated genes. Natural compounds...  相似文献   

东北地域农村朝鲜族民居实态调查研究     

李信昊  刘荣厚  张文基 《农业工程学报》2005,21(Z1):232-234

以位于延边朝鲜族自治州的朝鲜族聚居村龙山村为研究对象进行调查,阐明了朝鲜族的民居特性.农村民居由木结构土草房变为砖瓦房,平面结构中通间与双通屋较多,屋内间数逐年增加,继承了北朝鲜东北部的民居特色,并维持朝鲜民族传统居住习惯(火炕取暖和坐式生活),但在适应延边地理和气候特性后,逐渐转变成汉族、满族等多民族混居的文化形态.  相似文献   

Association between commercial and traditional sugar-sweetened beverages and measures of adiposity in Costa Rica     

Rhee JJ  Mattei J  Campos H 《Public health nutrition》2012,15(8):1347-1354

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Regulation of phospholipase C-gamma 1 by profilin and tyrosine phosphorylation.   总被引：58，自引：0，他引：58  

P J Goldschmidt-Clermont  J W Kim  L M Machesky  S G Rhee  T D Pollard 《Science (New York, N.Y.)》1991,251(4998):1231-1233

Epidermal growth factor and platelet-derived growth factor can stimulate the production of the second messenger inositol trisphosphate in responsive cells, but the biochemical pathway for these signaling events has been uncertain because the reactions have not been reconstituted with purified molecules in vitro. A reconstitution is described that requires not only the growth factor, its receptor with tyrosine kinase activity, and the soluble phospholipase C-gamma 1, but also the small soluble actin-binding protein profilin. Profilin binds to the substrate phosphatidylinositol 4,5-bisphosphate and inhibits its hydrolysis by unphosphorylated phospholipase C-gamma 1. Phosphorylation of phospholipase C-gamma 1 by the epidermal growth factor receptor tyrosine kinase overcomes the inhibitory effect of profilin and results in an effective activation of phospholipase C-gamma 1.  相似文献   

Stimulation of phospholipase C-gamma 1 membrane association by epidermal growth factor   总被引：18，自引：0，他引：18  

G Todderud  M I Wahl  S G Rhee  G Carpenter 《Science (New York, N.Y.)》1990,249(4966):296-298

Epidermal growth factor (EGF) treatment of A-431 epidermoid carcinoma cells elicited a redistribution of phospholipase C-gamma 1 (PLC-gamma 1) from a predominantly cytosolic localization to membrane fractions. The temporal coincidence of this redistribution with EGF stimulation of inositol phosphate formation and EGF increased phosphorylation of PLC-gamma 1 suggests that the membrane association of PLC-gamma 1 is a significant event in second messenger transduction.  相似文献