东北地域农村朝鲜族民居实态调查研究     

李信昊  刘荣厚  张文基 《农业工程学报》2005,21(13):232-234

以位于延边朝鲜族自治州的朝鲜族聚居村龙山村为研究对象进行调查，阐明了朝鲜族的民居特性。农村民居由木结构土草房变为砖瓦房，平面结构中通间与双通屋较多，屋内间数逐年增加，继承了北朝鲜东北部的民居特色，并维持朝鲜民族传统居住习惯（火炕取暖和坐式生活），但在适应延边地理和气候特性后，逐渐转变成汉族、满族等多民族混居的文化形态。  相似文献   

畜牧工程技术在民和企业中的运用     

孙希民  董泰丽  施正香  王朝元 《农业工程学报》2006,(Z2)

该文结合民和企业实例,论述了“工程防疫”长效防疫体系的建立,对企业发展和产品品质提升所产生的影响。认为将畜牧工程技术导入畜牧生产的全过程,有利于保障最大限度地发挥畜禽生产潜力,推进动物养殖业的技、工、贸一体化的产业化进程,有利于中国养殖业的健康可持续发展。  相似文献   

不同采收期对红星苹果虎皮病采后生理变化及品质的影响     

王坤范  周山涛  刘一和  刘增林 《中国农业大学学报》1983,(1):61-70

红星苹果虎皮病的发病率高低与采收期有着极其密切的关系。从1980—1981年试验的盛花后117天、127天、138天采收的红星苹果和1981—1982年试验的盛花后130天、140天及150天采收的红星苹果来看,都一致证明采收时期愈早,虎皮病的发病率愈高。塑料薄膜封闭包装有增加红星苹果虎皮病发病率的趋势。综合红星苹果在贮藏过程中生理和品质变化以及虎皮病的发病情况,认为北京地区的红星苹果以盛花后140天左右采收为宜,并初步认为果实采收时的乙烯浓度约为1ppm合适。  相似文献   

A rapid method for estimating microsomal oxidase activity in the housefly with (14C)parathion as substrate     

K S Rhee  F W Plapp 《Journal of agricultural and food chemistry》1974,22(2):261-264

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REVEGETATION OF DECOMPOSED GRANITE ROADCUTS IN KOREA: DEVELOPING DIGGER,EVALUATING COST EFFECTIVENESS,AND DETERMINING DIMENSIONS OF DRILLING HOLES,REVEGETATION SPECIES,AND MULCHING TREATMENT     

J.‐W. Lee  C.‐M. Park  H. Rhee 《Land Degradation \u0026amp; Development》2013,24(6):591-604

Decomposed granite roadcuts are difficult to revegetate after losing the topsoil and vegetation cover. We developed a new drilling machine, Digger, to efficiently drill six holes simultaneously on decomposed granite roadcuts to facilitate revegetation. The Digger consists of a base machine (0.7 m³‐level excavator) and a mounting body with six hydraulic motors instead of a bucket. We tested its performance on two roadcuts in southwest Korea using time‐motion studies, which showed that the Digger can drill 240 m² of decomposed granite roadcuts daily. The unit cost of the Digger was less than a half of other roadcut stabilization and revegetation techniques in Korea, making the Digger a cost‐effective revegetation technology. Field germination and growth tests were also conducted to identify appropriate diameter and depth of drilling holes, suitable revegetation species, and mulching treatment. We drilled holes with three different diameters and depths, filled the holes with a mixture of plant seeds and cultivated soil, applied mulching treatments (coir geotextile, shade net, and no mulching), and measured the germination and growth results at two field plots after 1 month and 1 year. The results showed that drilling diameter 10 cm and depth 10 cm were large enough to result in better plant germination and growth. Erosion control species, Poa pratensis L. and Eragrostis curvula (Schrad.) Nees, survived and grew better than native woody species. Coir geotextile improved the plant germination and growth. The time‐motion and revegetation results show that the Digger can be a promising technology to restore decomposed granite roadcuts. Copyright © 2013 John Wiley & Sons, Ltd.  相似文献   

Development of a high resolution melting (HRM) marker linked to genic male sterility in Capsicum annuum L.     

Heung‐Ryul Lee  Hye Jung An  Dong Cheol Yang  Soon Ho Choi  Hyoun‐Joung Kim  Han Gheol Rhee  Chee Hark Harn 《Plant Breeding》2012,131(3):444-448

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Evaluation of recombinant 28 kDa outer membrane protein of Brucella abortus for the clinical diagnosis of bovine brucellosis in Korea     

Lim JJ  Kim DH  Lee JJ  Kim DG  Min W  Lee HJ  Rhee MH  Chang HH  Kim S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(6):687-691

Brucella spp. are Gram-negative, facultative, intracellular coccobacilli that are pathogenic to a variety of mammals, including ruminants and humans. The conventional serological test for diagnosing brucellosis in cattle in Korea is the standard tube agglutination test. However, agglutination tests sometimes give false-positive results due to cross-reactions with other pathogens. The outer membrane proteins of Brucella species have been extensively studied for their immunogenicity and serodiagnostic applications. However, an application of B. abortus OMPs for serodiagnosis has not been successfully established. In this study, cloning and expression of B. abortus Omp28, a group 3 antigen, were accomplished by PCR amplification cloning into a pMAL expression system, and purification of a recombinant Omp28 (rOmp28). The immunogenicity of rOmp28 was confirmed by Western blot analysis with Brucella-positive bovine serum. To determine whether rOmp2 has a potential benefit for use in the serodiagnosis of bovine brucellosis, rOmp28-based ELISA and latex bead agglutination test were performed. B. abortus positive (n=122) or negative (n=88) from TAT were positive (118/122, 96.7%) or negative (84/88, 95.4%) in ELISA and were positive (94/122, 77%) or negative (71/88, 81.7%) in that the latex bead agglutination test, respectively. The sensitivity, specificity and accuracy were 96.7, 95.4, 96.2% in ELISA and 77, 80.6, 78.5% in latex bead agglutination test, respectively. These findings suggest that the rOmp28 of B. abortus might be a good candidate for developing serological diagnostic tools for bovine brucellosis.  相似文献   

Prevalence of Lawsonia intracellularis, Salmonella spp. and Eimeria spp. in healthy and diarrheic pet rabbits     

Lim JJ  Kim DH  Lee JJ  Kim DG  Kim SH  Min W  Chang HH  Rhee MH  Kim S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(2):263-265

A total of 170 fresh fecal samples (healthy; n=137, diarrheic; n=33) were collected from pet rabbits. By using PCR and formol-ether concentration method, a total 13/137 healthy rabbit feces were positive for L. intracellularis, 6/137 for Salmonella, and 13/137 for Eimeria. On the other hand, a total 17/33 diarrheic rabbit fecal samples were positive for L. intracellularis, 10/33 for Salmonella, and 21/33 for Eimeria. From these results, more than 20% of clinically normal and 97% of diarrheic rabbits were positive for single or concurrent infection of three pathogens. To the best of our knowledge, this is the first report to describe the prevalence of the microorganisms L. intracellularis, Salmonella and Eimeria in pet rabbits.  相似文献   

Protective effects of recombinant Brucella abortus Omp28 against infection with a virulent strain of Brucella abortus 544 in mice   总被引：1，自引：0，他引：1  

Jeong Ju Lim  Dong Hyeok Kim  Jin Ju Lee  Dae Geun Kim  Wongi Min  Hu Jang Lee  Man Hee Rhee  Suk Kim 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(3):287-292

The outer membrane proteins (OMPs) of Brucella (B.) abortus have been extensively studied, but their immunogenicity and protective ability against B. abortus infection are still unclear. In the present study, B. abortus Omp28, a group 3 antigen, was amplified by PCR and cloned into a maltose fusion protein expression system. Recombinant Omp28 (rOmp28) was expressed in Escherichia coli and was then purified. Immunogenicity of rOmp28 was confirmed by Western blot analysis with Brucella-positive mouse serum. Furthermore, humoral- or cell-mediated immune responses measured by the production of IgG1 or IgG2a in rOmp28-immunized mice and the ability of rOmp28 immunization to protect against B. abortus infection were evaluated in a mouse model. In the immunogenicity analysis, the mean titers of IgG1 and IgG2a produced by rOmp28-immunized mice were 20-fold higher than those of PBS-treated mice throughout the entire experimental period. Furthermore, spleen proliferation and bacterial burden in the spleen of rOmp28-immunized mice were approximately 1.5-fold lower than those of PBS-treated mice when challenged with virulent B. abortus. These findings suggest that rOmp28 from B. abortus is a good candidate for manufacturing an effective subunit vaccine against B. abortus infection in animals.  相似文献   

The yellow catfish, Pelteobagrus fulvidraco (Siluriformes) metallothionein cDNA: molecular cloning and transcript expression level in response to exposure to the heavy metals Cd, Cu, and Zn     

Kim JH  Rhee JS  Dahms HU  Lee YM  Han KN  Lee JS 《Fish physiology and biochemistry》2012,38(5):1331-1342

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