Assessment of genetic relationships in Mentha species     

S.P.S. Khanuja  A.K. Shasany  Alka Srivastava  Sushil Kumar 《Euphytica》2000,111(2):121-125

A set of 60 random primers was used to analyse 11accessions from six taxa of Mentha developed byCIMAP. These accessions were maintained in the nationalgene bank for medicinal and aromatic plants at CIMAP.A total of 630 bands could be detected as amplifiedproducts upon PCR amplification, out of which 589 werepolymorphic (93.5%). Further analysis of these RAPDprofiles for band similarity indices clearlydifferentiated five of the Mentha arvensis L.accessions from the rest. Among two accessions of Mentha spicata L. CIMAP/C33 could bedistinguished from CIMAP/C32. Mentha × gracilis Sole cv. cardiaca showed a muchhigher similarity with Mentha spicata L. as wellas Mentha arvensis L. which amongst themselvesshowed rather a greater distance indicating that Mentha × gracilis Sole cv. cardiaca might have evolved as a natural hybridbetween M arvensis L. and M. spicataL. In terms of uniqueness of amplified bands fordeveloping RAPD markers, it was observed that at taxalevel 298 bands were unique to one of the six taxa,singly amounting to 47.3% of total amplifiedfragments. Primers MAP 10 and 17 produced polymorphismonly in case of M. spicata L. and Menthaspicata L. cv. viridis while MAP 08 producedpolymorphic bands in all 4 other species than thesetwo. Similarly unique patterns were observeddifferentiating all six species and could be used asRAPD markers for differentiating Mentha species.  相似文献   

Synthesis,biological activities and SAR studies of novel 1‐Ethyl‐7‐methyl‐4‐oxo‐1,4‐dihydro‐[1,8]naphthyridine‐3‐carboxylic acid based diacyl and sulfonyl acyl hydrazines     

Nisha Aggarwal  Rajesh Kumar  Chitra Srivastava  Prem Dureja  J. M. Khurana 《Pest management science》2014,70(7):1071-1082

  相似文献   

Arbuscular Mycorrhizal (AM) Diversity in Prosopis cineraria (L.) Druce Under Arid Agroecosystems     

Neelam Verma  Jagadish Chandra Tarafdar  Krishna Kant Srivastava  Jitendra Panwar 《中国农业科学(英文版)》2008,7(6)

Arbuscular mycorrhizal (AM) fungi associated with Prosopis cineraria (Khejri) were assessed for their qualitative and quantitative distribution from eight districts of Rajasthan. A total of three species of Acaulospora, one species of Entrophospora, two species of Gigaspora, twenty-one species of Glomus, seven species of Sclerocystis and three species of Scutellospora were recorded. A high diversity of AM fungi was observed and it varied at different study sites. Among these six genera, Glomus occurred most frequently. Glomus fasciculatum, Glomus aggregatum, and Glomus mosseae were found to be the most predominant AM fungi in infecting Prosopis cineraria. Acaulospora, G. fasciculatum, Sclerocystis was found in all the fields studied, while Scutellospora species were found only in few sites. A maximum of thirty-six AM fungal species were isolated and identified from Jodhpur, whereas only thirteen species were found from Jaisalmer. Spores of Glomus fasciculatum were found to be most abundant under Prosopis cineraria.  相似文献   

Alterations in the epidermis of the carp,Labeo rohita (Cyprinidae: Cypriniformes), infected by the bacteria,Aeromonas hydrophila: A scanning electron microscopic,histopathological and immunohistochemical investigation     

Ayan Srivastava  Arup Mistri  Swati Mittal  Ajay Kumar Mittal 《Journal of fish diseases》2020,43(8):941-953

This study was carried out to comprehend the pathogenicity of the bacteria in the epidermis of Labeo rohita inoculated with Aeromonas hydrophila. Alterations in the histopathology of the epidermis were examined using scanning electron microscopy, light microscopy and the localization of iNOS and caspase 3 + ve cells by means of immunohistochemical methods. Skin samples obtained from infected fish at different intervals 2, 4, 6, 8 and 10 days showed significant changes in the cellular components of the epidermis. Epithelial cells often appeared hypertrophied with fragmented and loosely arranged microridges, and in the process of exfoliation. Mucous goblet cells increased significantly in density. Club cells showed degenerative changes, often with simultaneous confluence of adjacent cells and release of their contents. Increase in density of iNOS and caspase 3 + ve cells indicates inflammatory response and apoptosis. This study could provide valuable information on the pathogenesis of the disease, and disease outbreaks in farmed fish. Further, it could provide useful guidelines for fish farmers to take preventive measures for the control of the disease.  相似文献   

Culture of Catla catla in a lentic impoundment in Jammu     

J.B. Srivastava  S.K. Chowdhary 《Aquaculture (Amsterdam, Netherlands)》1979,18(3):283-285

In May 1977, fry of Catla catla was introduced for the first time by the Regional Research Laboratory, Jammu, for culturing in the inland waters of the region. One hundred tagged specimens and 250 untagged ones were stocked along with Indian major and Chinese carps in a rain- and partially sewage-fed impoundment having an area of 2.5 ha and depth ranging from 1.50 m to 3.25 m. Tagged specimens were caught by a cast net in July 1978. The average mean weight recorded was 4,750 g and the average mean length was 58.6 cm after 14 months. The largest specimen was 7,500 g in weight and 63,00 cm in length and the smallest 4,000 g in weight and 55.00 cm in length. The pond bloomed naturally with both phyto- and zooplankton throughout the year, and no supplementary feed or fertilizer was provided.  相似文献   

Gravity-fed drip irrigation system for hilly terraces of the northwest Himalayas   总被引：3，自引：1，他引：3  

P. Bhatnagar  R. Srivastava 《Irrigation Science》2003,21(4):151-157

Development of small tanks lined with low-density polyethylene film has provided irrigation facilities on the upland areas of hills on a limited scale. To utilise the scarcely available water, a gravity drip irrigation system was designed for the hilly terraces. The system had the capability to provide uniform emitter discharge throughout the command area spread on several terraces with varying elevations and irregular shapes. The design includes the estimation of the command area of the tank (runoff or low discharge spring-fed) using data on rainfall, evaporation and crop water requirement, and the hydraulics of the drip irrigation system. A useful and more pertinent star configuration of microtube layout with one lateral line feeding four rows was devised which had some advantages over the traditional layouts in terms of achieving the desired discharge rate, better handling of the system, appropriate water delivery, adjustment in spatial head variation due to friction loss in pipes as well as field slopes, and economic factors. Pilot testing of the system showed that the system worked efficiently, with field emission uniformity above 90%. Although the design criteria were developed for the topographical and climatic conditions of the mid-hills of the north-west Himalayas, they can easily be adapted for other locations.  相似文献   

Effect of boron and farmyard manure application on growth,yields, and boron nutrition of sunflower     

K. R. Sharma  P. C. Srivastava  D. Ghosh  M. S. Gangwar 《Journal of plant nutrition》2013,36(4-5):633-640

To examine the role of organic manure on boron (B) availability, a greenhouse experiment was conducted to study the effect of B and farmyard manure (FYM) levels on growth, yields, and B accumulation of sunflower (Helianthus annuus L.). Application of B or FYM individually increased the plant height, capitulum diameter, dry matter yields of seed and stalk and their B concentration and accumulation. The interaction of B and FYM levels had a significant influence only on B concentration of stalk and total B accumulation of sunflower. In the absence of B application, use of highest level of FYM (10 g kg^‐1 soil) significantly increased B concentration of stalk and total B accumulation by the crop while with B application, use of FYM at lower rates was effective. Application of B or FYM, especially at higher rates of the latter (5 and 10 g FYM kg^‐1 soil) increased the content of hot‐water soluble soil B in the soil after crop harvest. The interaction of B and FYM levels had no significant effect on the content of hot‐water soluble soil B. Application of FYM increased apparent availability of native and added B from soil to sunflower crops.  相似文献   

Retrieval of soil moisture by artificial neural network using X-band ground based data     

Abhishek Pandey  R. Prasad  J. K. Srivastava  S. N. Pandey 《Russian Agricultural Sciences》2012,38(3):230-233

Monitoring of soil moisture is very important to environmental studies, including hydrology, meteorology and their interactive fields. Today back propagation artificial neural networking is a well known and widely applied mathematical model for the remote sensing applications. For the soil moisture estimation an artificial neutral network (ANN) based algorithm is implemented and tested. The ANN model is calibrated (trained) and tested with the experimentally obtained data. The experimentally data is obtained by using X-band (9.5 GHz) scatterometer for different soil moistures viz. 10, 12, 18 and 22%. The measurement of the scattering coefficient was carried out over a range of incidence angle from 20° to 70° at the step of 5° for both the HH and VV polarization. Surface roughness (i.e. root mean square height) is taken constant as 0.5 cm for the whole experimentation. The performance of the ANN model is evaluated by the direct measured soil moisture and by the soil moisture estimated by the ANN model. Our work suggests that ANN modeling for such experimentation is a promising alternative for soil moisture estimation. The advantage of the ANN approach for soil moisture estimation is that it has potential for worldwide coverage.  相似文献   

Studies on mineralization and immobilization of nitrogen in soil     

M.C. Mundra  G.S. Bhandari  O.P. Srivastava 《Geoderma》1973,9(1):27-33

Mineralization and immobilization processes were studied in a soil treated with ammonium sulphate, calcium ammonium nitrate, urea, urea ammonium phosphate or suphala along with unhumified dung. The conversion of fertilizer nitrogen into non-KCI extractable fractions occurred within two weeks and was relatively rapid in the samples treated with urea and urea ammonium phosphate. The mineral nitrogen content during incubation studies was highest in calcium ammonium nitrate treated soils. Amino acid nitrogen appeared to be the main fraction involved in the immobilization and mineralization of nitrogen in soil. Dung behaved as an efficient nitrification inhibitor and slowed the release of nitrogen from fertilizers.  相似文献   

Chemical constituents of Kigelia pinnata twigs and their GLUT4 translocation modulatory effect in skeletal muscle cells     

Khan MF  Dixit P  Jaiswal N  Tamrakar AK  Srivastava AK  Maurya R 《Fitoterapia》2012,83(1):125-129

Phytochemical investigation of the ethanolic extract of twigs of Kigelia pinnata DC. afforded one new iridoid 7-hydroxy eucommiol (1), and nine known compounds (2-10). The structure of compounds was elucidated by extensive spectroscopic methods, including 1D, 2D NMR experiments and MS analysis. All these compounds were evaluated for GLUT4 translocation modulatory effect in skeletal muscle cells. Four of the tested compounds 1, 5, 6 and 7 showed significant stimulation of GLUT4 translocation to cell surface in skeletal muscle cells without any adverse effect on cell viability. Effect of these four compounds was concentration-dependent and comparable to standard drug rosiglitazone. These findings indicate that constituents of K. pinnata may provide leads for the therapeutics for insulin resistance and diabetes.  相似文献