Growth, photosynthetic pigments and photosynthetic activity during seedling stage of cowpea (Vigna unguiculata) in response to UV-B and dimethoate     

Vagish Mishra  Garima Srivastava  Sheo Mohan Prasad  Gerard Abraham 《Pesticide biochemistry and physiology》2008,92(1):30-37

UV-B (0.4 W m⁻²) irradiation and dimethoate (100 and 200 ppm) treatments, singly and in combination, declined the growth, photosynthetic pigment contents and photosynthesis (O₂ evolution and CO₂-fixation) of cowpea (Vigna unguiculata). Contrary to this, low concentration of dimethoate (50 ppm) caused stimulation on these parameters, while together with UV-B it showed inhibitory effects. Carotenoids (Car) showed varied responses. It was found that carbon-fixation (¹⁴CO₂) was more sensitive to both the stresses than photosynthetic oxygen evolution. Photosynthetic electron transport activity was reduced by both the stresses, however, 50 ppm dimethoate besides inhibiting photosystem II (PSII) and whole chain activity, showed slight stimulation in photosystem I (PSI) activity. The individual effect of two stresses on PSII activity was probably due to interruption of electron flow at oxidation side of PSII which extended to its reaction center following simultaneous exposure. A similar trend was also noticed in case of CO₂ liberation (measured as ¹⁴CO₂ release) in light and dark. Results suggest that dimethoate (100 and 200 ppm) and UV-B alone caused heavy damage on pigments and photosynthetic activity of cowpea, leading to the significant inhibition in growth. Further, the interactive effects of both the stresses got intensified. However, low concentration (50 ppm) of dimethoate showed stimulation, but in combination, it slightly recovered from the damaging effect, caused by UV-B.  相似文献   

Arbuscular Mycorrhizal （AM） Diversity in Prosopis cineraria （L.） Druce Under Arid Agroecosystems     

Neelam Verma Jagadish Chandra Tarafdar Krishna Kant Srivastava Jitendra Panwar 《中国农业科学(英文版)》2008,7(6)

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Comparative studies on chlorpyrifos and methyl parathion induced oxidative stress in different parts of rat brain: Attenuation by antioxidant vitamins     

Radhey S. Verma  Anugya Mehta  Nalini Srivastava   《Pesticide biochemistry and physiology》2009,95(3):152-158

Organophosphate (OP) pesticides are among the most widely used synthetic chemicals for controlling a wide variety of pests. Chlorpyrifos (o,o′-diethyl-o-3,5,6-trichloro-2-pyridyl phosphorothionate, CPF) is among the leading OP pesticides used extensively throughout the world including India while methyl parathion (o,o-di methyl-o-p-nitrophenyl phosphorothioate, MPT) another OP compound, widely used as insecticide and acaricide to control many biting or sucking pests of agricultural crops. Present study was carried out to compare the chronic toxicity of CPF and MPT, their potential to generate oxidative stress and ameliorating effects of antioxidant vitamins in brain of rats. Results of the present study clearly demonstrated that the oral exposure of CPF or MPT, generated oxidative stress in different parts of rat brain consequently accumulating malondialdehyde (MDA) and 4-hydroxynonanal (4HNE), the two major end products of lipid peroxidation, in all the three brain regions i.e. fore-, mid- and hind-brain. The levels of hydrogen peroxide (H₂O₂) were also increased in all the three brain regions when compared with control. CPF and MPT exposure caused decrease in the levels of reduced glutathione (GSH) and increase in the levels of oxidized glutathione (GSSG) in all the three brain regions. The increase in the levels of MDA, 4HNE, H₂O₂ and GSSG was less pronounced when CPF or MPT was given to the rats fed with a mixture of vitamin A, E and C. The present findings clearly show that oral intake of a mixture of vitamin A, E and C protects the rats from MPT or CPF induced oxidative stress and suggest that this treatment alleviates the toxicity of these pesticides to a greater extent.  相似文献   

Acidic Potassium Permanganate Chemiluminescence for the Determination of Antioxidant Potential in Three Cultivars of Ocimum basilicum     

Shivani Srivastava  Alok Adholeya  Xavier A. Conlan  David M. Cahill 《Plant foods for human nutrition (Dordrecht, Netherlands)》2016,71(1):72-80

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Evaluation of a recombinant LigB protein of Leptospira interrogans serovar Canicola in an enzyme-linked immunosorbent assay for the serodiagnosis of bovine leptospirosis     

Surya Sankar  Hiron M. Harshan  S.R. Somarajan  S.K. Srivastava 《Research in veterinary science》2010,88(3):375-378

A recombinant leptospiral lipoprotein, LigB, was evaluated for use in the diagnosis of bovine leptospirosis by enzyme-linked immunosorbent assay (rLigB IgG ELISA). The standard reference test (Microscopic agglutination test, MAT) of 200 serum samples from cattle suspected of leptospirosis showed that 95 (47.5%) samples had positive agglutination titres, which ranged from 100 to 1600. In rLigB IgG ELISA, 49% of the samples were positive. Sensitivity of IgG ELISA for 95 bovine sera, which had MAT titres of greater than or equal to 100, were 100%. ELISA showed a specificity of 97.1% with 105 bovine sera, which were negative at a 1:50 dilution in MAT for Leptospira interrogans serovars. The results of ELISA and MAT correspond very good. When analytical specificity of IgG ELISA was evaluated using bovine serum samples from animals showing the serum antibodies to other pathogens, no cross-reaction was observed. Thus the recombinant LigB IgG ELISA can be used instead of the MAT as an aid to the diagnosis of bovine leptospirosis.  相似文献   

Nutrient Indexing in “‘Kinnow” Mandarin (Citrus deliciosia Lour.× Citrus nobilis Tanaka) Grown in Indogangetic Plains     

A. K. Srivastava 《Communications in Soil Science and Plant Analysis》2016,47(18):2115-2125

Diagnosis and remediation of nutrient constraints in perennial fruit crop like citrus are the two important pillars of an effective nutrient management program. Efforts were made to develop nutrient indexing (NI) criteria based on generated leaf and soil analysis dataset for “Kinnow” mandarin (Citrus deliciosia Lour. × Citrus nobilis Tanaka) grown on illitic soils of Indogangetic plains (Entisol, Inceptisol, and Aridisol). NI through diagnosis and recommendation integrated system (DRIS) using leaf analysis data showed optimum value of leaf nutrient concentration as 2.22–2.32% nitrogen (N), 0.11–0.15% phosphorus (P), 1.10–1.41% potassium (K), 2.32–2.79% calcium (Ca), 0.38–0.61% magnesium (Mg), 22.4–58.3 ppm iron (Fe), 26.3–56.2 ppm manganese (Mn), 4.2–7.2 ppm copper (Cu), and 21.3–26.9 ppm zinc (Zn) vis-à-vis a fruit yield of 32.4–56.1 kg tree^?1. Using these NI criteria, Zn was observed as most deficient (64.7%) followed by Fe (61.5%), Mn (57.6%), N (96.1%), and P (38.5%) using percentage of orchards as basis. While, optimum NI (mg kg^?1) using soil analysis data was determined as 114.3–121.2 potassium permanganate (KMnO₄-N), 7.8–12.3 Olsen-P, 96.4–131.3 ammonium acetate (NH₄OAc)-K, 189.4–248.6 NH₄OAc-Ca, 72.3–89.9 NH₄OAc-Mg, 5.8–11.1, DTPA-Fe, 4.3–6.9 diethylenetriaminepentaacetic acid (DTPA)-Mn, 0.45–0.69 DTPA-Cu, and 21.3–26.9 DTPA-Zn for the optimum yield of 32.4–56.1 kg tree^?1. Soil analysis-based NIs displayed a good complementary with leaf analysis-based NIs evident from the diagnoses indicating Mn (52.2%) as most dominant constraint Zn (61.2%) followed by Mn (48.3%), N (41.2%), and P (35.6%). The recommended DRIS-based NIs would lay a scientific basis in formulating citrus fertilization program.  相似文献   

Disposition kinetics and dosage regimen of 2-formyl 1-methyl pyridinium oxime (2-PAM) in heifers     

A K Srivastava  H N Khanikor  J K Malik 《Acta veterinaria Hungarica》1988,36(3-4):251-256

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Properties of fructose-1,6-biphosphatase of fetal and adult goat liver     

R S Dhanotiya  R Bhardwaj  H S Srivastava 《Archiv fuer experimentelle veterinaermedizin》1987,41(4):479-485

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Pharmacokinetics following a single intravenous administration and a dosage regimen for sulfadoxine in buffalo calves (Bubalus bubalis)     

A. K. Srivastava  R. K. Chaudhary  M. S. Bal 《Veterinary research communications》1992,16(3):215-219

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Cloning and Characterization of <Emphasis Type="Italic">tbpA</Emphasis> Gene Encoding Transferrin-Binding Protein (TbpA) from <Emphasis Type="Italic">Pasteurella multocida</Emphasis> Serogroup B:2 (strain P<Subscript>52</Subscript>)     

Shivachandra SB  Kumar AA  Amaranath J  Joseph S  Srivastava SK  Chaudhuri P 《Veterinary research communications》2005,29(6):537-542

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